The sugar beet (Beta vulgaris) plant has in recent years been added to the Biofuel Industrial Strategy (Department of Minerals and Energy, 2007) by the South African government as a crop grown for the production of bio-ethanol. Sugar beet is commonly grown in Europe for the production of sucrose and has recently been cultivated in Cradock and the surrounding areas (Engineering News, 2008). The biofuel industry usually ferments the sucrose with Saccharomyces cerevisiae to yield bio-ethanol. However, researchers are presented with a critical role to increase current yields as there are concerns over the process costs from industrial biotechnologists. The beet factories produce a pulp by-product removed of all sucrose. The hemicellulose-rich pulp can be degraded by microbial enzymes to simple sugars that can be subsequently fermented to bio-ethanol. Thus, the pulp represents a potential source for second generation biofuel. The process of utilising microbial hemicellulases requires an initial chemical pre-treatment step to delignify the sugar beet pulp (SBP). An alkaline pre-treatment with ‘slake lime’ (calcium hydroxide) was investigated using a 23 factorial design and the factors examined were: lime load; temperature and time. The analysed results showed the highest release of reducing sugars at the pre-treatment conditions of: 0.4 g lime / g SBP; 40°C and 36 hours. A partial characterisation of the Clostridium cellulovorans hemicellulases was carried out to verify the optimal activity conditions stated in literature. The highest release of reducing sugars was measured at pH 6.5 – 7.0 and at 45°C for arabinofuranosidase A (ArfA); at pH 5.5 and 40°C for mannanase A (ManA) and pH 5.0 – 6.0 and 45°C for xylanase A (XynA). Temperature studies showed that a complete loss of enzymatic activity occurred after 11 hours for ManA; and 84-96 hours for ArfA. XynA was still active after 120 hours. The optimised lime pre-treated SBP was subsequently degraded using various combinations and percentages of C. cellulovorans ArfA, ManA and XynA to determine the maximal release of reducing sugars. Synergistically, the highest synergy was observed at 75% ArfA and 25% ManA, with a specific activity of 2.9 μmol/min/g protein. However, the highest release of sugars was observed at 4.2 μmol/min/g protein at 100% ArfA. This study has initiated the research within South Africa on SBP and its degradation by C. cellulovorans. Preliminary studies show that SBP has the potential to be utilised as a second generation biofuel source.

Thesis (MScFor (Forest and Wood Science))--University of Stellenbosch, 2010. / ENGLISH ABSTRACT: The discussion on climate change is leading to a re-evaluation of tree plantations in South Africa; prompting the adoption of forest bioenergy system as one of the cost effective ‘carbon mitigation options’. In an analysis of this changing paradigm, emphasis was placed on the socio-economic aspects of integrated commercial tree plantations and forest bioenergy systems with special attention to harvest residues recovery for bioelectricity production and construction and operation of a bioelectricity plant. The study also explored the direct and indirect benefits that adjacent communities derive from tree plantations in South Africa in order to determine the potential impact of integrated timber and bioelectricity production on rural livelihood and conventional forestry operations. Structured questionnaires and in-depth interviews were used in randomly sampling twelve villages on Mondi tree plantations in the Piet Retief and Iswepe areas of South Africa. Six villages from each area were selected; and a systematic random sampling of ten households per village was carried out. The possibility of using harvest residues from final clear felling from these plantations for bioelectricity production was examined. The study developed and described a scenario for a five megawatt bioelectricity generation facility, requiring an annual volume of 19,569.85 dry tonnes of residues as feedstock for its operation. The study revealed that adjacent rural communities to Mondi plantations in Piet Retief and Iswepe areas enjoy direct benefits such as employment opportunities, utilization of harvest residues, utilization of non-timber resources, and free accommodation. Indirect benefits that these communities enjoy include: free farmland and graze-land and various social benefits. Issues of concern and dislike such as: lack of electricity; poor health and sanitation and transportation problems were also identified. Using NPV and IRR, the study estimated the economic impacts of integrated pulpwood and bioelectricity production, compared to conventional pulpwood production operation. The study concluded that integrated pulpwood and harvest residue recovery for bioelectricity production is a profitable means of producing renewable energy. The approach was found to increase the profitability of conventional forest operations. / AFRIKAANSE OPSOMMING: Besprekings rondom klimaatsverandering lei tot ‘n her-evaluasie van boom plantasies in Suid Afrika wat aanleiding gee tot die aanvaarding van bio-energie stelsels as een van die koste effektiewe “koolstof versagtende opsies”. In ‘n ontleding van hierdie paradigma verandering, is klem geplaas op die sosio-ekonomiese aspekte van die integrasie van boom plantasies en bos bio-energie stelsels. Spesiale aandag is gegee aan onginningsafval herwinning vir bio-energie produksie en die konstruksie en werking van ‘n bio-elektriese kragsentrale. Die studie ondersoek ook die direkte en indirekte voordele wat gemeenskappe, aangrensend aan boom plantasies in Suid Afrika verkry, om sodoende die potensiële effek van geintegreerde hout en bio-elektriese produksie op landelike lewensbestaan en konvensionele bosbou operasies te bepaal. Gestruktureerde vraelyste en indiepte onderhoude is gebruik om ‘n lukraakte steekproef van twaalf dorpies op Mondi boom plantasies in die Piet Retief en Iswepe areas van Suid Afrika uit te voer. Ses dorpies in elke area is gekies en ‘n sistematiese lukraakte steekproef van tien huishoudings per dorpie is uitgevoer. Die moontlikheid om ontginningsafval van finale kaalkap van hierdie plantasies vir bio-elektrisiteit te gebruik is ook ondersoek. Die studie het ‘n senario ontwikkel en beskryf van ‘n vyf megawatt bio-elektriese kragsentrale wat ‘n jaarlikse volume van 11,708 droë ton ontginningsafval benodig as voermateriaal vir kragopwekking. Die studie het getoon dat aangrensende landelike gemeenskappe langs Mondi plantasies in die Piet Retief en Iswepe areas direkte voordele soos werksgeleenthede, gebruik van ontginningsafval, gebruik van nie-hout hulpbronne en gratis akkommodasie geniet. Indirekte voordele wat gemeenskappe geniet sluit in gratis toegang to landbou grond en weiding, sowel as sosiale voordele. Probleemfaktore waarmee hulle saamleef is ‘n gebrek aan elektrisiteit, swak gesondheids en sanitasie dienste en vervoerprobleme. Deur die gebruik van NPV en IRR analitiese metodes is die ekonomiese impak van geintegreerde pulphout en bio-elektrisiteits produksie bepaal en vergelyk met konfensionele pulphout produksie. Die gevolgtrekking is dat geintegreerde pulphout en ontginningsafval herwinning vir bio-elektrisiteit produksie ‘n winsgewende manier van hernubare energie produksie is. Die benadering kan die winsgewendheid van konfensionele bosbou operasies verbeter.

The research was undertaken to investigate the impact of the Tshivhase Tea Estate on the space economy of Venda, the people and area ofMapate, and Duthuni, among whom the Tea Estate is established. The approach is based on principles. Principles of development theory are combined with appropriate spatial models. The development reality of Venda, together with many other development projects are analysed. Does the tea estate address the rural poverty problem? Findings are presented from a case study of Tshivhase and this proved the lack of growth and development impulses to alleviate the poverty of the rural people among whom it is located. Development is considered in terms of its possible simultaneous diffusion of economic activity and modernisation in all four dimensions of the spatial system : political, socio-cultural, economic and physical. / Department of Geography / M.A. (Geography)

The research was undertaken to investigate the impact of the Tshivhase Tea Estate on the space economy of Venda, the people and area ofMapate, and Duthuni, among whom the Tea Estate is established. The approach is based on principles. Principles of development theory are combined with appropriate spatial models. The development reality of Venda, together with many other development projects are analysed. Does the tea estate address the rural poverty problem? Findings are presented from a case study of Tshivhase and this proved the lack of growth and development impulses to alleviate the poverty of the rural people among whom it is located. Development is considered in terms of its possible simultaneous diffusion of economic activity and modernisation in all four dimensions of the spatial system : political, socio-cultural, economic and physical. / Department of Geography / M.A. (Geography)