Thesis (Ph. D.)--University of Missouri-Columbia, 1998. / Typescript. Vita. Includes bibliographical references (leaves 154-161). Also available on the Internet.

Thesis (M.S.)--University of Missouri-Columbia, 2003. / Typescript. Vita. Includes bibliographical references (leaves 98-100). Also available on the Internet.

Thesis (M.S.)--University of Missouri-Columbia, 2003. / Typescript. Vita. Includes bibliographical references (leaves 98-100). Also available on the Internet.

Thesis (Ph. D.)--University of Missouri-Columbia, 1998. / Typescript. Vita. Includes bibliographical references (leaves 154-161). Also available on the Internet.

Microarray technology like that of recombinant DNA and the polymerase chain reaction is a foundational component of modern biotechnology. There are many aspects in the manufacture of a DNA chip and each serves as a contributing variable to the overall performance of the device. Commercial production of microarrays commenced before a basic understanding of the physical and chemical processes that govern the many variables was attained and the role of each on the performance of microarrays was understood. The consequence of this has been non-optimal performance of this technology and variable results from microarray analyses. Recent studies have shown that part of the variability in the use of commercially available microarrays relates to insufficient control of the surface chemistry within a microarray spot and between spots. Although the ideal characteristics of a microarray surface have been identified, existing surfaces and the ongoing proliferation of alternative ones do not address these sufficiently in order to reduce the sources of error arising from the surface of a microarray. Hence, the application of physico-chemical techniques in the development of microarrays surfaces can assist in improving the performance of these devices. / Thesis (PhDApSc(MineralsandMaterials))--University of South Australia, 2006.

Thesis (M.S.)--Rochester Institute of Technology, 1994. / Typescript. Includes bibliographical references (leaves 62-65).

Resumo: Esta dissertação de mestrado trata do estudo da caracterização estrutural, e de propriedades óticas e mecânicas do dietilenoglicoldimetiléter (DIGLIME) polimerizado via plasma. Este tipo de plasma produz filmes poliméricos com características físicas, químicas e biológicas semelhantes àquelas observadas em poli(óxido de etileno)- (PEO) e polietileno-glicol (PEG), que são polímeros produzidos através de processos químicos convencionais. Os polímeros a plasma foram obtidos usando descargas de rádio-freqüência operando em 13,56 MHz. Os parâmetros do processos de deposição utilizados foram potência de 10 a 40W e pressão de operação de 120 a 440 mTorr. A estrutura molecular dos polímeros foi estudada por espectroscopia infravermelha. De acordo com os resultados obtidos, baixas pressões e potências de RF produzem filmes poliméricos com características tipo PEO. A tensão mecânica residual nos filmes poliméricos foi investigada pelo método da deflexão de um feixe de laser. Os resultados indicaram tensões compressivas em todos os polímeros e boa estabilidade em função do tempo de envelhecimento. As propriedades óticas dos polímeros a plasma, como coeficiente de absorção, gap ótico e índice de refração foram investigadas através de espectroscopia ultravioleta-visível. O índice de refração calculado é próximo de 1,5 e o gap ótico decresce de 5,0 para 3,5 eV quando a potência de rádio-freqüência é aumentada de 10 para 40W . / Abstract:This Mastering Dissertation deals with the study of structural characterization mechanical and optical properties of the diethyleneglycoldimethylether (diglime) polymerized by plasma. This kind of plasma produces polymer films with interesting physical, chemical and biological characteristics likely those observed in polyethylene-oxide (PEO) and polyethylene-glycol (PEG) that are polymers produced by conventional chemical processes. The plasma polymer films were obtained using 13.56 MHz radio-frequency discharges. The process parameters were RF power from 10 to 40W and operation pressure from 120 to 440 mTorr. The molecular structure of the films was investigate by infrared spectroscopy. According to the obtained results, low pressures and RF power discharges, produce polymer films with PEO-like characteristics. The residual mechanical stress in the films was investigated by the laser beam deflection method. The results indicated compressive stresses in all films and good stability as a time function. The optical properties of the plasma polymers such as absorption coefficient, optical gap and refractive index were investigated using ultraviolet-visible spectroscopy. The calculated refractive index is near 1.5 and the optical gap decreases from 5.0 to 3.3 eV as the RF power is increased from 10 to 40W. / Orientador: Rogerio Pinto Mota / Coorientador: Nilson Cristino da Cruz / Banca: Elson de Campos / Banca: Munemasa Machida / Mestre

The diploma work deals with the preparation of biocompatible hydrophilic layers from a propane-butane mixture by plasma polymerization. Subsequent characterization of the layers was performed using both biological (antibacterial tests and biocompatibility test) and physico-chemical diagnostics (SEM, OES and surface energy determination). The theoretical part presents the basic principle of dielectric barrier discharge, its use and various types, including surface dielectric barrier discharge (SDBD), which was used during deposition. Next, cell cultures, cell morphology and clarification of the term biocompatibility were described. At the end of the theoretical part, all used during the experimental part of the diagnostics were also described. In the experimental part, the procedure of deposition of hydrophilic layers and the overall apparatus, which was used during deposition, were presented. Prior to the characterization of the samples, the discharge was diagnosed using optical emission spectroscopy. Furthermore, the level of hydrophilicity of the layers and their elemental composition on the surface was determined. Biological tests for biocompatibility and antibacterial properties provided information on the viability of the cells on the layers and their antibacterial effects.

Theoretical part of diploma thesis was focused on the search of the state of knowledge in the area of plasma, plasma polymerization and characterization of thin films. Plasma-enhanced chemical vapor deposition (PECVD) was described in the experimental part together with selected analytical techniques. The technology with high level of reproducibility was reached by precise control of deposition conditions, monitoring of plasma, and analysis of plasma products using mass spectrometry. The obtained results demonstrated that the elemental composition, chemical structure, optical and mechanical properties of films were influenced by effective power used.

No description available.