This thesis deals with the quality of the internal environment of wooden buildings, especially the quality of the indoor environment interior loaded VOC emissions. The main goal of this work is to analyze the influence of relative humidity constructions on the quality of the indoor environment in the region Mountains. As assessed buildings were elected four wooden buildings, one of which is a frame structure, the second is half-timbered, and the other two are made with timber construction.Sampling methodology VOC emissions from the interior of the investigation based on the standard DIN EN ISO 16000 1 and 5. Sampling polluted indoor air was performed using a membrane pump Gilian LFS - 113DC, the collected air samples were collected in metal sorption tubes that are filled with TENAX TA sorbent. Indoor Environmental Quality assessed based constructions of wood was monitored during one calendar year, with the influence of the season on emissions of VOC(Fall, Winter, Spring ).Part of this work was to also assess the impact of age of the building and its material composition on the emission of volatile organic compounds assessed based constructions of wood.Detected concentrations of VOCs elected representatives were subsequently compared with the limit concentration by the Ministry of Health Decree No. 6/2003 Coll. , Laying down the health limits of chemical, physical and biological indicators of residential rooms inside certain buildings.

The thesis discusses the possibilities of determination of chlorophenols in beer. A review of literature describes the complex properties of chlorinated phenols. Summarizes their physical, chemical and toxicological properties and characterizes their possible inputs into the environment. In the experimental part is the main theme of the determination of chlorophenols in samples of small breweries and beers called as home brew. These are various types of beer, in which the amount of chlorophenols determined using solid phase extraction (SPE) using gas chromatography with flame ionisation detector (FID). Another part of the experiment is aimed at demonstrating the formation of chlorophenols in beer by simulating conditions in the production of beer in the pilot plant. Chlorphenols amount of boiled beer is again determined by gas chromatography. Samples of beers are compared with each other in the content of chlorophenols. It is evaluated sectional representation monitored chlorophenols and their quantity in the analyzed samples of beer.

This thesis is focused on determination of fatty acids in processed and/or processed cheese analogues using gas chromatography with FID detection. Characterization of fatty acids, various methods for lipid extraction and the possibilities of determination of fatty acids are described in the theoretical part. Simple and rapid method for the determination of fatty acids in natural and/or processed cheese was optimized and validated in the experimental part. The modified Folch method (by mixture of chloroform - methanol) was selected for lipid extraction from the samples. The standard method (ISO 5509), using a methanol solution of potassium hydroxide, was selected for lipid esterification. Type of the stationary phase and parameters of the column, flow of carrier gas, temperature of the injector, injection technique, temperature of the detector and temperature program were optimized. Calibration curves were constructed from five calibration points (in the concentration range of 0.01 - 60 g.ml-1), all correlation coefficients R2 were higher than 0,99. The limits of detection and quantification of individual fatty acids ranged from 0.002 - 6 g.ml-1. The repeatability of the method was good, all the relative standard deviations of the peaks were below 9 %. The optimized and validated method was applied to selected samples of processed cheese analogues. In total five types of analogues containing different kinds of fat (butter, apricot, linseed, black currant and grape seed oils) were analyzed. In samples 34 fatty acids were identified based on a comparison of retention times of standards.

Master thesis is concerned with gas turbines from Siemens company. The thesis consists of two main parts. In the first part describes the different types of turbines, their characteristics and use in practice. The following is a detailed description of components and systems the biggest industrial gas turbine SGT-800. The second part discusses about the necessary steps for commissioning turbine. These particulars are managing software, testing, commissioning and phasing of the turbine generator to the distribution network. The conclusion is devoted to the example of calculating return investment on the overall gas turbine project.

KROUPA Petr, Technical requirements for welded constructions in gas industry. Graduation theses., Mechanical engineering, Department 2307 Metal Forging Technology, Specialization 02 Forming, Welding, 2. , 2. graduate, school- year 2007/2008. FSI VUT Brno, ÚST Department of Metal Forging Technology, Mai 2008, page NO. 85, picture NO 16, chart NO 11, supllement NO 8 The graduation thesis, drew up with in the frame of engeneering studies department M2307, submits a koncept of a analysis requirements in light of specification, law and order governments at disposition, transaction and verification welding at production construction in gas manufacture. It is sight on welding construction from low karbon steels in the area gas manufacture in light of requirements Czech state specification and European specification, that have was given entrance CR to the European union. Specification with be related to demand on making and documentation, system notation steel, system notation steel, technology welding, production fundamentals for transaction gas construction, destructive and non-destructive testing needed in applying the provision gas construction.

This thesis deals with the possibility of the integration of gas microturbine into a laundry energy system. The thesis further describes the system of professional laundry care and used equipment in industrial practice. It presents several possible scenarios of operation and compares their energy costs.

The theme of the work is the integration of gas microturbines in industrial plants. The aim is to extend the application potential of this technology by the design of the integration into the process in which this technology is not exploited yet. This process is a professional laundry service. Professional laundry services have been selected as a representative of the generally known process, which is interesting in terms of energy intensity. Combining of progressive cogeneration technology and enhanced industrial process brings a promising application potential. The work focuses on small industrial laundries to 500 kg of processed linen per shift. As a cogeneration unit a gas microturbine Capstone C30 has been used. The microturbine has an adequate power due to the process. Complete laundry operation and a gas microturbine is a part of the equipment of the Laboratory of energetically demanding processes in the NETME Centre. The main contribution of this work is to design a system for utilization of flue gas heat for heating of the main input currents to the laundry room, a hot water for washing machines and warm air for drying in the dryers. The integration of the gas microturbine made to suit specific process can be a major cost-saving measures with positive impacts on the economy of operation.

This master thesis deals with problems of determining operational and safety risks at gas boiler room in office building with total output of 4×49 kW. The gas boiler room is equipped with four boilers Geminox THRi 10-50. The aim of this master thesis is to determine the potential operational and safety risks of gas boiler room. The master thesis is divided into several parts, when the first part is describing the current situation with some theoretical knowledge and risk analysis. This part also outlines possible solutions for identification and quantification of risks to the gas boiler room. The specific risk factors are determined at other part of master thesis and there is also their analysis, which is solved by cause and effect diagram and by Failure Mode and Effect Analysis. The specific precautions are suggested for chosen risks in final part of master thesis.

- 4 - Abstrakt The aim of this diploma thesis was to determine the possibility of identifying the protein and lipid additives, such as blood, gelatine, curd, lard and eggs, in the model samples of lime based mortars using Raman spectroscopy and gas chromatography- mass spectrometry. It was proved that the method of Raman spectroscopy can determine the presence of organic additives in the order of one weight percent. The gas chromatography enables to detect lipid additives from the order of hundredths weight percent. Unambiguous resolution of protein additives can be done in the samples with the concentrations up to the order of one weight percent of the additives.

Chemical substances are transferred between bumblebees of the species Bombus terrestris during their mating behavior. These substances are present mainly on the cuticle. The aim of this thesis was to identify and quantitatify differences in the amount of the compounds among defined groups of bumblebees - mated, non-mated and attempting to mate. Cuticular extracts were obtained by washing of the whole bumblebees bodies in hexane. These extracts were analyzed by using a two-dimensional gas chromatography with the time of fly mass spectrometr (GCxGC-TOF/MS). Cuticular hydrocarbons were identified and semi-quantified. Double bounds locations were determined in heptacosene and nonacosene. Five compounds were differed in the examined groups - β-springene, α-springene, dihydrofarnesyl-oleate, palmityl-palmitate and palmityl- palmitoleate. By comparing of the amount of these compounds differences between defined groups of bumblebees were determined. Analysis of the cutilar extracts showed that mated and non-mated bumblebees can be distinguished. Furthermore, there were statistically significant differences in the amounts of identified compounds in all defined groups of bumblebees. Key words: two-dimensional gas chromatography, Bombus terrestris, cutilar extract