No description available.

Thesis (M. Ph.)--Chinese University of Hong Kong. / Bibliography: l. 73-78.

Dissertation submitted in partial compliance with the requirements for the Master’s Degree in Technology: Homoeopathy, Durban University of Technology, 2010. / Provings are an essential element in the development of the homoeopathic medicine knowledge base and scope of practice. There are many South African indigenous plants and animals that can play a role in homoeopathy but need to be proved first. Several indigenous snake venoms have already been proved as homoeopathic remedies, so the proving of Dendroaspis angusticeps venom expands this particular knowledge base. Aim The aim of this study was to determine the effect of a homoeopathic dose of the venom of the Dendroaspis angusticeps on healthy individuals in order to elicit and document the resulting mental and physical symptomotology, so that it may be prescribed to those that require it based on the Law of Similars. The other aims of this study were to compare the proving symptoms of Dendroaspis angusticeps 30CH to the toxicology of Eastern Green Mamba venom and to analyze the remedy picture in relation to the doctrine of signatures. The thirtieth centesimal potency of the remedy was hypothesised to produce clearly observable signs and symptoms in provers (healthy volunteers). The symptoms obtained from the proving were also compared to the doctrine of signature analysis of Dendroaspis angusticeps to help clarify the nature of this snake and to expand the remedy picture of the substance and facilitate our understanding of the remedy in light of the toxicology of the venom and the doctrine of signatures. vii Methodology The homoeopathic proving of Dendroaspis angusticeps 30CH was carried out as a randomised, double blind, placebo-controlled trial. The proving population consisted of 30 provers. Twenty four of the provers were in the experimental group and they received the potentised snake venom. Six provers were in the control group and they received the placebo. As this was a double blind, placebo-controlled trial, neither the individuals taking part in the study nor the researcher had any knowledge of who received placebo or the active remedy. Provers were also unaware of the substance being proven or the potency in which it was prepared. Results Provers recorded their symptoms in a journal over a period of 5 weeks and were monitored regularly by the researcher. At the completion of the proving period, the researcher collected all the recorded data and each journal was assessed by the researcher to determine the suitability of the recorded symptoms. The symptoms were then translated into the language of the materia medica and the repertory and a remedy picture was formulated. Thereafter a comparison was made to the nature of the snake by means of the doctrine of signatures and the toxicology of the venom as an attempt to broaden the repertoire of our knowledge of the action of the remedy. Provers experienced a wide range of symptoms particularly on the mental and emotional spheres including feelings of powerful assertion and confidence; cheerful and excited energy; spiritual and prayerful feelings; seductive, sensual and extravagant mannerisms; desire to dance and heightened energy; clairvoyant natures and desires to be in nature as well as pronounced irritability, anger and sadness. Other symptoms included desire to be alone and withdrawn feelings; quarrelsome natures; desire to kill; deceitful ways; intolerance to injustice; poor concentration; thoughts of the past; confusion and antagonism of the will and anxious thoughts. Delusions, fears and thoughts of death also manifested in provers. There was a marked polarity of symptoms as: Anger, rage, irritability vs calmness / spirituality; Sadness / depression vs cheerfulness / joy; Poor concentration / forgetful vs mental power / mental clarity; Alone / withdrawn vs desire to be touched, caressed; and Prostration, exhaustion vs energetic. On the physical sphere there were marked symptoms produced in the head area with a wide range of headaches. Eye symptoms were also vast in the inflammation, heaviness and ptosis of the eyes. Throat symptoms manifested as pharyngitis and sore throat. Toxicological symptoms included vertigo, ptosis, pain in different areas, inflammation, vomiting, blurred vision, slurred speech, difficulty breathing and difficult swallowing. Back pain and lower back pain were also key symptoms. Sleep difficulties and sleepiness were experienced by provers. Dream symptoms were the most prevalent in this proving. Dreams were repetitive in provers in the dreams of changing places and being in many places and unfamiliar places. There were marked dreams of sexuality, death, spirituality, friendships, colours, snakes, weddings, fighting and killings and past recollections. Conclusion Dendroaspis angusticeps 30CH thus did produce clearly observable signs and symptoms in healthy volunteers as hypothesised. The correlation of the proving symptoms to the toxicology of the venom and the doctrine of signatures also illustrated the themes of this remedy and aided in development of the remedy picture for this new addition to the knowledge base of indigenous South African substances and their homoeopathic application. / M

Thesis (M.Tech.: Homoeopathy)-Dept of Homoeopathy, Durban Institute of Technology, 2004 xiv, 154 leaves : ill. ; 30 cm / This study was conducted by administering Naja mossambica 30CH (a homoeopathic remedy derived from the venom of the Mozambican spitting cobra) to healthy individuals in order to elicit and document the resulting mental and physical symptomology. These symptoms were compared to the toxicology of Naja mossambica venom as well as a doctrine of signatures analysis of the snake in order to expand and clarify the remedy picture. Existing knowledge of the venom toxicology gives a clear indication of the organs and body systems that the substance has an affinity for. Many poisonous substances used homoeopathically rely heavily on inferences made from the toxicology of the substance, as much of the gross pathology in the symptom picture cannot safely be elucidated in a proving. The aim of this study was to determine the sphere of action of Naja mossambica by utilising symptoms obtained from the proving and from the toxicology of the venom. This was done in order to determine the remedy's usefulness in a homoeopathic clinical setting by expanding our understanding of the substance and thereby facilitating the treatment of disease based on the law of similars.

Submitted in partial compliance with the requirements of the Master’s Degree in Technology: Homoeopathy, Durban University of Technology. 2013. / This study was a homoeopathic drug proving of Bitis atropos 30CH (derived from Berg adder venom) with a subsequent comparison of the proving symptoms to known venom toxicology and existing remedies from the materia medica, that on repertorisation, yielded the greatest similarities in the Mental, General, Physical and unique symptomatology of Bitis atropos. Methodology : The proving was carried out in the form of a double-blinded, placebo controlled trial on healthy subjects who were administered the proving substance or placebo. The resultant influence of this substance on the health of provers (i.e. symptoms produced) was recorded in journal format and formed the materia medica and ultimately the clinical indications thereof according to the Law of Similars. Twenty eight healthy consenting provers who meet the inclusion criteria (Appendix B), were randomly split into two groups, one being the experimental group comprising 22 provers, and the other a placebo control group comprising 6 provers. This was further split between the researcher and co-researcher, each responsible for 11 provers receiving verum and 3 receiving placebo. The researchers and the individual provers were unaware of their respective group allocation and the provers were unaware of the identity of the proving substance. The fresh venom sourced from a wild, Berg adder, was processed according to the German Homoeopathic Pharmacopoeia (Appendix G) to produce the 30CH Homoeopathic potency thereof. Six lactose powders were dispensed to each prover (either placebo or verum) and taken sublingually three times a day or until the onset of symptoms. Symptoms were recorded by the provers in journals over 4 weeks and were closely supervised by the researcher. When the symptoms subsided, the combined journals were collected, collated, analysed, interpreted and validated. Accepted symptoms were converted to materia medica and Repertory format. Results : The proving yielded a total of 903 rubrics, of which 18 were newly created. The systems mostly affected were Dreams, Mind, Head and Eye. Comparison of proving symptoms to that of venom toxicology, as seen in case studies of envenomation by Bitis atropos, yielded similar results, as the sensations experienced in provers closely matched that of known venom toxicology. On repertorisation of the proving symptoms, the existing remedies that were closely related were Sepia officinalis, Lachesis mutus and Argentum nitricum. Further repertorisation of toxicological symptoms indicated a further relation to Belladonna, Natrum muriaticum and Hyoscyamus niger. Conclusion : Clearly observable signs and symptoms were produced by healthy provers in response to administration of Bitis atropos 30CH, in addition there was a significant degree of similarity between proving symptoms and that of known toxicology of the crude substance. The researcher identified Sepia officinalis, Lachesis mutis and Argentum nitricum as the three most similar existing homoeopathic remedies and a detailed comparison thereof was conducted. A further repertorisation of the toxicological symptoms of envenomation by the snake, yielded the remedies Belladonna, Natrum muriaticum and Hyoscyamus niger which were also compared to Bitis atropos.

Abstract not available

Thesis (M.Tech.: Homoeopathy)-Dept of Homoeopathy, Durban Institute of Technology, 2004 xiv, 154 leaves : ill. ; 30 cm / This study was conducted by administering Naja mossambica 30CH (a homoeopathic remedy derived from the venom of the Mozambican spitting cobra) to healthy individuals in order to elicit and document the resulting mental and physical symptomology. These symptoms were compared to the toxicology of Naja mossambica venom as well as a doctrine of signatures analysis of the snake in order to expand and clarify the remedy picture. Existing knowledge of the venom toxicology gives a clear indication of the organs and body systems that the substance has an affinity for. Many poisonous substances used homoeopathically rely heavily on inferences made from the toxicology of the substance, as much of the gross pathology in the symptom picture cannot safely be elucidated in a proving. The aim of this study was to determine the sphere of action of Naja mossambica by utilising symptoms obtained from the proving and from the toxicology of the venom. This was done in order to determine the remedy's usefulness in a homoeopathic clinical setting by expanding our understanding of the substance and thereby facilitating the treatment of disease based on the law of similars.

Made available in DSpace on 2014-06-11T19:24:15Z (GMT). No. of bitstreams: 0 Previous issue date: 2012-09-10Bitstream added on 2014-06-13T18:20:27Z : No. of bitstreams: 1 gasparotto_vpo_me_botfm.pdf: 823732 bytes, checksum: 72bbebb42d3239b78a28393a5aa3b0b7 (MD5) / Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) / Universidade Estadual Paulista (UNESP) / O estudo avaliou a viabilidade in vitro do biomaterial “Selante de Fibrina derivado de veneno de serpente” (SF), como arcabouço para células tronco mesenquimais (CTMs) de ratos. O SF é um material caracterizado como adesivo biológico, e foi produzido e fornecido pelo Centro de Estudos de Venenos e Animais Peçonhentos, CEVAP, Brasil. As CTMs foram coletadas a partir da medula óssea de fêmures e tíbias de ratos e foram caracterizadas por meio de citometria de fluxo com auxilio de marcadores positivos: CD 44 e CD 90 (CTMs) e marcador negativo: CD 34 (células tronco hematopoiéticas). Cultivos foram induzidos para diferenciarem-se em linhagens específicas (osteogênico, condrogênico e adipogênico). Para avaliação do crescimento in vitro e a viabilidade celular em conjunto ao biomaterial foram utilizadas microscopia óptica de luz invertida, microscopia de fluorescência e microscopia eletrônica de varredura e transmissão. O SF em contato com as CTMs não induziu a diferenciação espontânea para as linhagens osteogênica, condrogênica e adipogênica. As diferentes técnicas de avaliação microscópica utilizadas mostraram que o SF foi capaz de realizar a captura e manutenção das CTMs e houve interação das células com o interior e superfície do biomaterial. Portanto, a coleta, o cultivo e a caracterização das CTMs de ratos foram possíveis. O SF mostrou-se eficiente como arcabouço biológico e interagiu com as células tronco mesenquimais mantendo-as viáveis, oferecendo-se como uma ferramenta de uso clínico-cirúrgico alternativa para processos regenerativos visando terapias mais eficientes / This study evaluated the in vitro viability of biomaterial Fibrin Sealant (FS) derived from snake venom as a scaffold for rat mesenchymal stem cells (MSCs). The FS is characterized as a biological adhesive material, and is produced and was supplied by the Center for the Study of Venoms and Venomous Animals, CEVAP, Brazil. MSCs were collected from the bone marrow of femurs and tibias of rat and were characterized using flow cytometry with CD 44 and CD 90 positive markers (MSC) and CD34 negative marker (mononuclear stem cells). Cultivations were induced to differentiate into specific cell lineage (osteogenic, chondrogenic and adipogenic). To evaluate the in vitro growth and cell viability with the biomaterial were used inverted light optical microscopy, fluorescence microscopy and scanning electron microscopy and transmission. The SF did not cause the spontaneous differentiation in contact with MSCs to osteogenic, chondrogenic and adipogenic lineage. Different microscopy techniques showed that the SF was able to accomplish the capture and maintenance of MSCs and there was interaction with the cell interior and surface of the biomaterial. Finally, the collection, cultivation and characterization of rat MSCs were possible. The SF was effective as a biological scaffold and interacted with the MSCs keeping them viable offering itself as a tool for clinical and surgical alternative providing clinical and surgical therapies more efficient for regenerative processes

Orientador: Rui Seabra Ferreira Junior / Coorientador: João Ferreira de Lima Neto / Banca: Fernanda da Cruz Landim Alvarenga / Banca: Alexandre Leite Rodrigues de Oliveira / Resumo: O estudo avaliou a viabilidade in vitro do biomaterial "Selante de Fibrina derivado de veneno de serpente" (SF), como arcabouço para células tronco mesenquimais (CTMs) de ratos. O SF é um material caracterizado como adesivo biológico, e foi produzido e fornecido pelo Centro de Estudos de Venenos e Animais Peçonhentos, CEVAP, Brasil. As CTMs foram coletadas a partir da medula óssea de fêmures e tíbias de ratos e foram caracterizadas por meio de citometria de fluxo com auxilio de marcadores positivos: CD 44 e CD 90 (CTMs) e marcador negativo: CD 34 (células tronco hematopoiéticas). Cultivos foram induzidos para diferenciarem-se em linhagens específicas (osteogênico, condrogênico e adipogênico). Para avaliação do crescimento in vitro e a viabilidade celular em conjunto ao biomaterial foram utilizadas microscopia óptica de luz invertida, microscopia de fluorescência e microscopia eletrônica de varredura e transmissão. O SF em contato com as CTMs não induziu a diferenciação espontânea para as linhagens osteogênica, condrogênica e adipogênica. As diferentes técnicas de avaliação microscópica utilizadas mostraram que o SF foi capaz de realizar a captura e manutenção das CTMs e houve interação das células com o interior e superfície do biomaterial. Portanto, a coleta, o cultivo e a caracterização das CTMs de ratos foram possíveis. O SF mostrou-se eficiente como arcabouço biológico e interagiu com as células tronco mesenquimais mantendo-as viáveis, oferecendo-se como uma ferramenta de uso clínico-cirúrgico alternativa para processos regenerativos visando terapias mais eficientes / Abstract: This study evaluated the in vitro viability of biomaterial Fibrin Sealant (FS) derived from snake venom as a scaffold for rat mesenchymal stem cells (MSCs). The FS is characterized as a biological adhesive material, and is produced and was supplied by the Center for the Study of Venoms and Venomous Animals, CEVAP, Brazil. MSCs were collected from the bone marrow of femurs and tibias of rat and were characterized using flow cytometry with CD 44 and CD 90 positive markers (MSC) and CD34 negative marker (mononuclear stem cells). Cultivations were induced to differentiate into specific cell lineage (osteogenic, chondrogenic and adipogenic). To evaluate the in vitro growth and cell viability with the biomaterial were used inverted light optical microscopy, fluorescence microscopy and scanning electron microscopy and transmission. The SF did not cause the spontaneous differentiation in contact with MSCs to osteogenic, chondrogenic and adipogenic lineage. Different microscopy techniques showed that the SF was able to accomplish the capture and maintenance of MSCs and there was interaction with the cell interior and surface of the biomaterial. Finally, the collection, cultivation and characterization of rat MSCs were possible. The SF was effective as a biological scaffold and interacted with the MSCs keeping them viable offering itself as a tool for clinical and surgical alternative providing clinical and surgical therapies more efficient for regenerative processes / Mestre