Since Pecan Creek receives sewage wastes from an obviously inadequate disposal system, it seemed possible that it could receive pathogenic bacteria. The fact that these might be present in numbers sufficient to cause epidemics, provided the proper natural vectors were present, stimulated an interest in and the pursuance of this study.

The purpose of this study was to compare the simultaneous effects of the water soluble fraction (WSF) of JP-4, a common military and civilian jet fuel, on survival, ventilatory rate, preference-avoidance behavior, and blood enzymes and ions of the bluegill sunfish (Lepomis rnacrochirus) to determine possible interrelationships and which procedures might be most descriptive of sublethal stress. The dynamic 96-h LCSO for WSF JP-4 was 26.2% (percent of the maximum soluble amount of JP-4). A concentration of 5.1% WSF JP-4 caused a detectable shift in ventilatory functions (rate and amplitude); this concentration was equivalent to the 96-h LCOl. Fish did not display a strong preference-avoidance reaction when exposed to WSF JP-4. Some avoidance occurred at 3.5% and 4.9% WSF JP-4. At concentrations near the 96-h LCSO fish appeared to lose their ability to detect WSF JP-4, indicating potential for fish not to avoid lethal levels of WSF JP-4. Few significant changes in whole and serum blood parameters were measured in fish exposed to 13% WSF JP-4; at near lethal concentrations changes were primarily attributed to osmoregulatory failure. Therefore such changes in blood parameters would not be useful to validate or confirm exposure to WSF JP-4. There were few significant changes in gill and liver histology of fish exposed to WSF JP-4 other than an increase in size or number of lipid vacuoles in the liver of exposed fish. / Ph. D.

Sampling data of many surface water bodies in Broward County tend to reveal abnormal levels of bacterial concentrations for total coliform, Escherichia coli and Enterococcus. Given the presence of septic systems in geohydrologically sensitive areas throughout the County, several studies (Morin 2005 and Bocca 2007) suggested a correlation in the high bacterial densities and the septic sites. / Includes bibliography. / Thesis (M.S.)--Florida Atlantic University, 2014. / FAU Electronic Theses and Dissertations Collection

Thesis (MPH)--University of Limpopo, 2005 / Water and sanitation inadequacy is still an environmental health challenge in several regions worldwide and a billion people lack access to safe water, while 2.4 billion people have inadequate sanitation [2]. Assessment of water quality by its chemistry includes measures of elements and molecules dissolved or suspended in water. Commonly measured chemical parameters include arsenic, cadmium, calcium, chloride, fluoride, total hardness, nitrate, and potassium [16]. Water quality can also be assessed by the presence of waterborne microorganisms from human and animals’ faecal wastes. These wastes contain a wide range of bacteria, viruses and protozoa that may be washed into drinking water supplies [21]. Three villages were selected for water quality analysis, based on their critical situation regarding access to water and sanitation: namely, Matlou, Sekuruwe and Taolome villages, situated in the Mogalakwena Local Municipality within the Waterberg district of the Limpopo Province, South Africa. A proposal was written to the Province of North Holland (PNH) and was approved for funding to start with the implementation of those projects, with 20% of each village’s budget allocated for water quality research [26]. This was a cross sectional, analytical study to investigate the chemical and microbial quality of water in Matlou, Sekuruwe and Taolome villages. The study was also conducted to explore methods used by household members to store and handle water in storage tanks. Water samples were collected and analysed according to the standard operating procedures (SOPs) of the Polokwane Municipality Wastewater Purification Plant in Ladanna, Polokwane City of South Africa. The questionnaire used was adopted from the one used for cholera outbreak in the Eastern Cape Province of South Africa. Results show that water from all sources in all the villages had increased total hardness concentration. Water from the borehole in Matlou village had increased number of total coliform bacteria. There were increased total and faecal coliform bacteria in storage tanks samples from Matlou village. Water samples from reservoirs in Sekuruwe and Taolome villages did not test positive for any microbial contamination. Water from xiv informally connected yard taps in Sekuruwe village had increased total coliform bacteria, while increased total and faecal coliforms were found in households’ storage tanks. Water samples from communal taps in Taolome village had minimal number of total coliform bacteria, while water from storage tanks had both increased total and faecal coliform bacteria. Matlou village was the only place with increased nitrate concentration at the households’ storage tanks. While all the villages had microbial contamination, Taolome village had the least number of coliform bacteria in water samples from households’ storage tanks as compared to Matlou and Sekuruwe villages. It is concluded that water from sources supplied by the municipalities are safe to be consumed by humans while water from informally connected taps and households’ storage tanks are not safe to be used without treatment. It is recommended that a health and hygiene education package be prepared for all the villages, so that handling of water from the main source into their storage tanks can be improved. Secondly, it is recommended that water in all sources be treated for total hardness and water in storage tanks in Matlou village be treated for nitrate. Thirdly, it is recommended that water be accessed everyday of the week, so that people do not use unsafe water supplies. / The Province of North Holland, Netherlands.

The concentrations of various forms of nitrogen and phosphorus in the main stream and selected tributaries of Pirron Yallock Creek, southwestern Victoria were examined over a two-year period. Exceedingly high levels of both nutrients were found within a particular tributary, while generally high nutrient levels were observed throughout the catchment. The speciation results indicated that dairy effluent was the primary source of nutrients to Pirron Yallock Creek. A palaeolimnological study was undertaken to determine the impact of European settlement upon waterway nutrient concentrations within the region. The palaeolimnological study focused primarily on the diatom flora preserved within the sediments of nearby Lakes Corangamite and Bullen Merri. Lack of preservation of diatoms within the sediments of Lake Corangamite resulted in no water quality inferences for this lake. The preserved diatoms within Lake Bullen Merri suggested an increase in trophic status of this lake during the last 500 years. While a change in the diatom flora of Lake Bullen Merri was evident, it was not possible to differentiate the impact of European settlement upon nutrient status of this waterway from long-term trophic status change. In light of the particularly high nutrient concentrations observed in Pirron Yallock Creek, improved nutrient management strategies are proposed for the catchment. These strategies, which consider current nutrient management activities, are predominantly focussed on the dairy industry, which occupies the majority of the catchment.

We explain a new method of detecting non point source fecal contamination using a PCR based method called Touchdown Polymerase Chain Reaction (TD-PCR). Using genetic markers particular to general, ruminant and human Bacteroides- Prevotella genes, we identified presence in both fresh and salt water environments. Water samples from four sites were collected at approximate 2-week intervals for a year. Samples were analyzed for total coliforms, fecal coliforms, E. coli, and the presence of general, ruminant and human Bacteroides-Prevotella markers. We compared the odds of recovering each PCR marker between sites. We investigated the relationship between rainfall and recovery of PCR markers. Finally, we compared the sensitivity of the PCR methods to standard public health methods. / Graduation date: 2003

With congressional passage of the BEACH Act in October of 2000, Coastal and Great Lakes states were mandated to assess coastal recreation waters for the application of ambient water quality standards. This research encompasses two components involved in applying the BEACH Act statues to Oregon. The first component was to select beach sites in Oregon. The second component involves applying bacterial recreational water standards to select Oregon beaches. Using the guidelines provided by the United States Environmental Protection Agency (EPA), this study develops a method to appraise Oregon marine recreational waters taking into account the following factors: use, available information, pollution threats, sanitary surveys, monitoring data, exposure considerations, economics, and development. In an effort to protect the public from swimming-associated illness attributable to microbial pollution, 24 beaches were identified in Oregon. Of these, 19 beaches were classified as tier 1, or high priority, and five sites were classified as medium priority, or tier 2. Future studies should be directed at ascertaining the beach lengths utilized by Oregon marine recreators since this is an important parameter in targeting bacterial monitoring. Ongoing monitoring of these 24 sites is warranted and new information could be used to update beach tier levels in Oregon. In the second phase of this study, bacterial monitoring data was used for comparison to recreational water quality standards. In October of 2002, the Oregon Department of Environmental Quality (ODEQ) sampled 26 beaches for enterococci and Escherichia coli (E. coli) densities. Of the water sampled from all 26 beach sites, nine exceeded s single sample maximum density of 104 enterococci colony forming units (cfu) per 100 milliLiters (mL). The Oregon beach with the highest exceedance occurred at Otter Rock's South Cove where the enterococci concentration was 4352 most probable number (MPN)/100 mL. A comparison of the 26 sampled beaches to ODEQ's estuarine E. coli standard of 406 organisms/100 mL resulted in two beaches with exceedances. Otter Rock at South Cove had the highest E. coli concentration at 1850 MPN/100 mL. Based on the limited data used in this study, should Oregon adopt the enterococci standard in lieu of the current ODEQ estuarine E. coli standard, more beaches will have exceedances of the recreational water standard. Additional bacterial monitoring is warranted to further characterize the nature and extent of the problem in Oregon. To protect the health of the marine recreating public, future Oregon marine water quality studies should delineate the "no swim" zone around creeks and model the impacts of rainfall on beach sites. / Graduation date: 2004

Few studies, and none in Oregon, have examined the presence and change of water quality parameters over time in popular natural swimming areas. This information is necessary to better understand water quality and risk of illness from either fecal contamination or cross-infection from other swimmers. The purpose of this study was to quantitatively measure bacterial and selected physical and chemical parameters, and collect background information for changes to the current state water quality criteria. Five natural swimming areas in Linn, Benton and Polk counties were chosen and sampled biweekly for physical, chemical, and bacterial parameters over a nine week period from June 28 to August 31, 1996. The results showed differences in bacterial levels over the sampling period which often varied by degrees of magnitude between sites. For example, the range in Escherichia coli levels was between 0 and 1000 colonies/100mL sample for two sites on the same sampling day. Similarly, the range in fecal coliform levels was between 5 and 500 colonies/100mL sample. The daily colony counts at each site exceeded the state standards at least 10% of the time for E. coli and 21% of the time for fecal coliform. At the most popular swimming site, Montieth Park. the fecal coliform regulatory levels were exceeded 79% of the time and E. coli levels were exceeded 42% of the time. This may be due to turbidity, high bather load, or a broken sewer line. The 30 day log mean of these values shows consistently elevated fecal coliform problems only at Montieth Park. For the other sites, the log means did not exceed the state and federal regulatory limits for fecal coliform or E. coll. This raises questions about which estimates should be used to assess public health risk. None of the other parameters in this study were correlated with bacterial counts, so it appears that none of these factors is solely responsible for elevated bacterial levels. Further testing should be done at Montieth Park to determine the cause of the elevated fecal coliform levels. Any follow-up studies should test several different indicator organisms in addition to E. coli for comparison and assessment of their relationship to public health risk. / Graduation date: 1997

Graduation date: 1995

Diss. (sammanfattning) Uppsala : Sveriges lantbruksuniv., 2009. / Härtill 4 uppsatser.