Thesis (M.S.) in Marine Bio-Resources--University of Maine, 2008. / Includes vita. Includes bibliographical references (leaves 95-104).

No description available.

Benthic foraminifera are exceptional organisms with distinctive features that allow for interpretation of both past and present environmental conditions. Some benthic foraminifera are widely distributed while some are restricted to specific environments due to their way of life. Foraminiferal assemblages south of Biscayne Bay and north of Cape Canaveral have previously been investigated; however, a gap exists in data covering a transitional zone along the Florida coast between the tropical waters of the western Atlantic and the cooler coastal waters along the North American coast. The purpose of this study was to collect baseline data on the benthic foraminifera of the small marine environment off of Pompano Beach that falls within this zone. This environment has a very particular relict reef system that includes a near-shore ridge complex, the unique foraminiferal assemblage of which has not been documented. Thirteen rubble samples were collected from this near shore ridge complex between October 2013 and April 2015 from depths of 2.5m – 9m. Abundances and diversity indices were calculated, and multivariate analysis and SHEBI analysis carried out to summarize baseline data for the area. Substrate types and seasonal collections were compared with foraminiferal abundances to determine if benthic foraminifera diversity varied between the four substrate types found on the near-shore ridge and between wet and dry seasons in Florida. Results revealed a variation in abundances for both substrates and seasons with the dominant genera being Quinqueloculina, Laevipeneroplis, and Archaias. Multivariate analysis displayed dissimilarities between substrates colonized by corals and those that were uncolonized. Comparison of studies from surrounding areas revealed fewer, however similar, species and different dominant genera. Overall, this area has proven to be a different environment compared to surrounding coastal areas and merits further investigation. / Includes bibliography. / Thesis (M.S.)--Florida Atlantic University, 2016. / FAU Electronic Theses and Dissertations Collection

Thesis(M.S.)--Auburn University, 2007. / Abstract. Vita. Includes bibliographic references.

Thesis--Auburn University, 2009. / Abstract. Includes bibliographical references (p. 40-43).

Aquaculture is currently the fastest growing sector of protein production, and is expected to overtake the harvest of wild fisheries. Limitations in nutrition, specifically fatty acid nutrition, are preventing even more dramatic growth of many species of commercial importance. Currently, much of the research involving fatty acids examines requirements as being correlated to thermal guilds (warm vs. cool water) or salinity tolerance (marine vs. freshwater). However, recent studies have revealed the potential for trophic level to be as much, if not more, influential in determining fatty acid requirements of a species. As such, two feeding trials were conducted to determine the requirements of two species of different trophic levels (Oreochromis niloticus and Trachinotus carolinus) based on C18 PUFA vs. LC-PUFA. Nile Tilapia, O. niloticus, exhibited similar growth regardless of the inclusion of C18 PUFA or LC-PUFA, however, tissue fatty acid profiles were influenced per the diet provided. As such, it was concluded that Nile Tilapia exhibit the capacity to effectively synthesize LC-PUFA from C18 PUFA as is seen in many species that occupy low trophic levels. Florida Pompano, T. carolinus, did not exhibit any significant differences in growth regardless of the diet provided, but numerical differences indicated benefits towards inclusion of dietary LC-PUFAs. Similar to O. niloticus, tissue fatty acid profiles were significantly affected by dietary treatment. Based on numerical differences in growth performance and significant differences in tissue fatty acids, it was concluded that Florida Pompano show a typical carnivorous requirement for LC-PUFA.

Dissertação(mestrado)-Universidade Federal do Rio Grande, Programa de Pós-Graduação em Aqüicultura, Instituto de Oceanografia, 2008. / Submitted by Cristiane Silva (cristiane_gomides@hotmail.com) on 2012-08-10T12:19:02Z No. of bitstreams: 1 Luiza.pdf: 183731 bytes, checksum: 0b5ee5a01d33ee313ae943f665008553 (MD5) / Approved for entry into archive by Bruna Vieira(bruninha_vieira@ibest.com.br) on 2012-08-21T17:28:37Z (GMT) No. of bitstreams: 1 Luiza.pdf: 183731 bytes, checksum: 0b5ee5a01d33ee313ae943f665008553 (MD5) / Made available in DSpace on 2012-08-21T17:28:37Z (GMT). No. of bitstreams: 1 Luiza.pdf: 183731 bytes, checksum: 0b5ee5a01d33ee313ae943f665008553 (MD5) Previous issue date: 2008 / O presente estudo foi conduzido com o objetivo de estimar a toxicidade aguda da amônia (N-NH3) e do nitrito (N-NO2-) para juvenis do pampo Trachinotus marginatus (0,86 ± 0,21 g) em diferentes salinidades: 5, 10 e 30‰. Os juvenis foram capturados na Praia do Cassino (Rio Grande, RS), levados para o laboratório e aclimatados nas salinidades desejadas durante 10 dias.Durante este período eles foram alimentados em excesso diariamente. A temperatura e o fotoperíodo foram fixados em 24ºC e 12C:12E, a concentração de oxigênio dissolvido e o pH foram medidos diariamente. Grupos de cinco peixes foram expostos a cinco concentrações de amônia (concentrações reais entre 0,28 a 3,53 mg/L N-NH3) e nitrito (concentrações reais entre 24,8-191,1 mg/L N-NO2-) durante 96 h, mais um grupo controle para cada salinidade (três repetições cada). Os testes foram conduzidos em um sistema semi-estático com renovação diária total dos meios experimentais. As concentrações letais medianas e seus respectivos intervalos de confiança (95%) foram estimados com base nos dados de mortalidade registrados nas diferentes concentrações testadas, utilizando o software Trimmed Spearman Karber Method. As CL50 - 96 h de amônia foram: 0,66 (0,53-0,81; 5‰), 1,87 (1,65-2,12; 10‰) e 1,06 (0,94-1,20; 30‰) mg/L N-NH3. As CL50 - 96h para nitrito foram 39,94 (36,39 -43,84; 5‰), 116,68 (112,52-121,00; 10‰) e 37,55 (20,91-67,44; 30‰) mg/L N-NO2-. A toxicidade aguda da amônia e nitrito para o pampo foi afetada pela salinidade. Os resultados do presente estudo mostraram que pampos criados em um ambiente isosmótico são menos sensíveis a N-NH3 e N-NO2-. A tolerância para a amônia é comprometida em salinidades reduzidas, enquanto que a toxicidade do nitrito é similar nas salinidades 5 e 30‰ / The present study was conducted to estimate the acute toxicity of ammonia nitrogen(NH3-N) and nitrite-nitrogen (NO2- -N) to juvenile pompano Trachinotus marginatus (0.86 ± 0.21 g) at different salinity levels: 5, 10 (equivalent to its isosmotic point), and 30‰. Fish were acclimated to the different salinities for 10 days and fed in excess daily. Groups of five fish (0.86 ± 0.21 g) were exposed to five concentrations of NH3-N and NO2- -N for 96 h plus control groups for each salinity in three replicates where no toxicant was added, measured concentrations ranged from 0.28 to 3.53 mg NH3-N/L and 24.8 to 191.1 mg NO2- -N/L. Tests were run using a standard semi-static system with 100% daily renewal of water and toxicants. The results were based on mortality data registered in different concentrations tested, using the software Trimmed Spearman Karber method. The median-lethal concentrations (LC50) after 96h of exposure to NH3-N were 0.66 (0.53-0.81), 1.87 (1.65-2.12) and 1.06 (0.94-1.20) mg NH3-N/L for 5‰,10‰, and 30‰. The LC50 -96 h to NO2- -N were 39.94 (36.39-43.84), 116.68 (112.52-121.00) and 37.55 (20.91-67.44) mg NO2- -N/L for ‰, 10‰, and 30 ‰. Acute toxicity of NH3-N and NO2- -N to pompano was affected by salinity. Results of the present study show that pompano reared at an isosmotic environment are less sensitive to NH3-N and NO2- -N. Tolerance to NH3-N is compromised at reduced salinities, while toxicity of NO2- -N is similar at 5 and 30 ‰

The main objective of this thesis was to gain new insights in three of Florida’s high value food and stock enhancement finfish nutrition (Common snook, Florida pompano and red drum) to improve larviculture protocols. The main bottleneck in snook production is the extremely low larval survival rate, which hinders subsequent research. This work first focused on the source of the larvae by looking at potential nutritional deficiencies in captive broodstock. The lipid composition of wild and captive common snook broodstock were compared to identify disparities and gain the information necessary for the formulation of a suitable diet for captive stocks. Results showed that captive snook lipid content was significantly higher than that of wild fish. However, cholesterol and arachidonic acid (ARA) levels were significantly lower compared to wild broodstock, with potential impact on steroid and prostaglandin production, reproductive behavior and gametogenesis. Eggs from captive broodstock incorporated high docosahexaenoic acid (DHA) and eicosapentaenoic acid (EPA) levels and low ARA levels. Consequently, ARA/EPA ratio in captive eggs was more than half of that in wild eggs (2.3 ± 0.6 and 0.9 ± 0.1 respectively), with a probable perturbation in eicosanoid production and adverse effects on embryo and larval development. The large differences observed between wild and captive broodstock most likely contributed to the reproductive dysfunctions observed in captive snook broodstock (e.g. incomplete oocyte maturation, low milt production and poor egg quality). In addition, the presence of hydrocarbons was detected in the liver of most of the wild snook sampled. This requires further investigation to identify the source of the contamination, monitor a potential impact on reproductive performances and protect the species habitat. Another major bottleneck in marine fish rearing occurs during the transition from endogenous feeding to exogenous feeding, with mass mortality events linked to inadequate first feeding diets. To gain insight on the early fatty acid requirements and mobilization of pompano and snook larvae, the pattern of conservation and loss of fatty acids from the yolk sac during the endogenous feeding period and subsequent starvation was studied. In both species, fatty acids were utilized as an energy source after hatching. Mono-unsaturated fatty acids were catabolized, while saturated and poly-unsaturated fatty acids were conserved. High levels of arachidonic acid (ARA) in pompano and snook eggs (respectively 2.44 ± 0.1 and 5.43 ± 0.3 % of total fatty acids), as well as selective retention in the unfed larvae, suggested a high dietary requirement for this fatty acid during the early stages of larval development. The effect of an ARA supplementation was therefore investigated in snook larvae at the rotifer feeding stage. Larvae receiving the supplementation did incorporate higher levels of ARA, and DHA/EPA and ARA/EPA ratios were successfully modified to match those observed in wild eggs. No significant improvements in growth or survival were observed, however the success in fatty acid profile modification suggest a possible impact of the supplementation on a longer period of time and a possible effect on stress resistance. Probiotics have been shown to enhance larval performances of several species and this strategy was therefore investigated to evaluate a potential impact on Florida pompano, red drum and common snook larvae. The effect of a commercial mix of Bacillus sp. was studied on larval survival, growth and digestive enzyme activities. Larvae were fed either live feed enriched with Algamac 3050 (Control), Algamac 3050 and probiotics (PB), or the previous diet combined with a daily addition of probiotics to the tank water (PB+). Microbiological analyses were performed at the end of the pompano trial. Numbers of presumptive Vibrio sp. were low and not statistically different between treatments, therefore no additional microbiological analyses were performed on the system. At the end of the pompano and snook trial, standard lengths of larvae from the PB and PB+ treatments were significantly greater than for the control larvae. For both pompano and snook, trypsin specific activity was higher in PB and PB+ larvae compared to the control larvae. Similarly, alkaline phosphatase activity was higher for the pompano larvae fed the PB and PB+ treatments and for the snook larvae fed the PB+ treatment compared to the control larvae. No enhancement of growth or digestive enzymes activities was observed in red drum larvae. Yet, no negative effects were noticed and a longer trial period and the study of additional parameters could reveal different effects. In all three species, survival was not affected by the supplementation; however, stress exposure should be further investigated as the supplementation may strengthen the larvae, especially pompano and snook larvae where the Bacillus sp. supplementation appears to promote growth through an early maturation of the digestive system. Another key challenge in marine fish larval rearing resides in weaning the larvae onto dry micro-diets. This step is commonly concurrent with larvae metamorphosis into juveniles, with extensive morphological and physiological changes that are likely to influence nutritional requirements. In the present project, three microdiets were tested on weaning of Florida pompano larvae: Otohime, Gemma and a reference diet LR803. The experimental system was stocked with 11-day-old larvae, which were co-fed micro-diets and live food from 11 dph to 17 dph then micro-diets only until 28 dph. Survival from 11 dph to 28 dph was similar for all treatments, with an average of 33 %. At the end of the trial, the Gemma larvae were significantly longer and heavier than larvae fed the other diets. Fatty acid composition of the diets and larvae varied significantly between treatments. The Gemma larvae incorporated the lowest amount of eicosapentaenoic acid (EPA), docosahexaenoic acid (DHA) and arachidonic acid (ARA). However, they presented the highest DHA/EPA and ARA/EPA ratios, supporting the concept that the proportions of polyunsaturated fatty acids are of greater importance than their absolute amount. Results from the enzyme analysis showed that fishmeal is a suitable main source of protein for Florida pompano larvae and demonstrated the full functionality of the pancreas at 16 days post hatch. These results provide the basis of a suitable weaning diet for pompano larvae and indicate the possibility of a weaning time prior to 16 days post hatch, which is of high interest in commercial production. Overall, this research provides new data on common snook, pompano and red drum nutritional requirements with results that can be directly applied to help overcome major bottlenecks in the hatchery phase and improve rearing protocols.