In the continental United States, mean surface air temperature is expected to increase by up to 5°C within 100 years. With hotter temperatures, leaf budbreak is expected to occur earlier in forests, and leaf area is expected to increase in locations where temperature is limiting. The response of plant photosynthesis to hotter temperatures is less certain; plant productivity could increase or decrease. Past studies have found intraspecific variation in the responses of forest tree productivity, phenology, canopy leaf area, and leaf isoprene emission to warming, which all influence carbon uptake and yield for agricultural tree species; it is therefore important to understand not only how hot climates affect carbon uptake and biomass production between different tree species, but also in different genotypes of the same species. We conducted a common garden study at the Biosphere 2 research center near Oracle, AZ, USA. We created a hybrid poplar plantation of 168 trees, which were planted as cuttings in January 2013. The trees used in this study are comprised of 5 distinct genotypes of Populus deltoides × trichocarpa from a range of average annual air temperatures. We measured photosynthetic capacity, leaf phenological timing, canopy leaf area and aboveground woody biomass in 2014 growing season, and leaf isoprene emission in the 2015 growing season. We observed a strong effect of genotype on aboveground woody biomass increment, implying strong local adaptation to the home range and limited phenotypic plasticity in terms of physiological and biometric responses to high temperature environments. Our study suggests that genotypes from hotter home ranges are able to maintain photosynthetic capacity and canopy leaf area late into the growing season, despite high temperatures, and thus produce more aboveground woody biomass. This study may have implications for agricultural management—as temperatures warm where managers currently grow hybrid poplar for agricultural or other purposes, the genotypes from those home ranges would likely have reduced yield; managers could investigate the use of genotypes from home ranges with higher average temperatures to replace the vulnerable local varieties.

Remediation of trichloroethylene (TCE) is a major priority for many contaminated sites all over the industrialized world including Hill Air Force Base, UT (HAFB). Phytoremediation as part of a cleanup strategy is an appealing option, and trees at HAFB have been studied to this end. Trees have also been used to delineate groundwater plumes because the passive uptake of organic contaminants by trees generally results in a direct relationship between tree and groundwater TCE concentrations if the trees are using the contaminated groundwater. However, the concentrations of plant-produced TCE metabolites can vary greatly. It was hypothesized that the endophytic microbial community present may be affecting the fate of TCE within these trees. This study was designed to determine if the microbial community present within hybrid poplar trees would change in response to TCE exposure. Trees were grown in a greenhouse to reduce environmental variability. Concentrations of TCE, its degradation products, and its metabolites were then measured in these trees. DNA was extracted from the tree tissues and amplified to determine the quantity of microbial DNA. Diversity of this DNA was determined by fragment analysis. Data were analyzed to determine if there was an effect of TCE treatment on the microbial community composition in the trees. Results showed that all tissues of dosed trees contained TCE. Metabolism of TCE to trichloroacetic acid (TCAA) and trichloroethanol (TCEtOH) in tree tissues was observed by the accumulation of these metabolites. Microbial DNA results revealed that TCE treatment reduced both the quantity and diversity of endophytic bacteria and fungi in several cases. Multivariate statistical analyses also showed that the endophytic microbial community shifted in response to TCE treatment. The endophytic microbial communities present in the hybrid poplar trees of this study were high in concentration and diversity, both of which were affected by TCE treatment. Endophytic bacterial concentrations were observed at over 109 copies/g with diversities of 70+ genetically distinct organisms. Decreases in these values with the addition of TCE showed that the community dramatically changed in some cases, but was able to more quickly adapt to TCE addition in other cases. The effects of these endophytic microorganisms associated with plants should therefore be included when phytoremediation is considered.

Selected PCB congeners (PCB 52, 77, and 153) singly and in mixtures were spiked and aged in soil microcosms and subsequently planted with switchgrass (Panicum virgatum) or poplar (Populus deltoids x nigra DN34). The planted reactors showed significantly greater reductions in PCB parent compounds when compared to unplanted systems after 32 weeks, both in single congener exposures and when all three congeners were present in a mixture. There was evidence of reductive dechlorination in both planted and unplanted systems, but higher concentrations of transformation products were observed in the planted systems than the unplanted. Although planted systems resulted in greater biotransformation, this improvement in PCB-reduction was not the result of plant uptake but rather was due to transformations occurring in the root rhizosphere. Parent PCB congeners were transformed by reductive dechlorination resulting in successively less chlorinated PCB congeners. These dechlorination products accounted for approximately all of the molar mass of parent compound lost. Based on the transformation products, reductive dechlorination pathways are proposed for rhizospheric biotransformation of PCB 52, 77, and 153. Results suggest that PCB 52 transformation proceeds through PCBs 18 and 9 down to monochlorinated PCB 1. Biotransformation of PCB 77 occurs through the intermediaries PCB 35 and 37. The pathway for the rhizospheric transformation of PCB 153 is through PCB 101 and PCB 99. This study provides insight into rhizosphere biotransformation pathways for reductive dechlorination in marginally aerobic,intermittently flooded soil as evidenced by a mass balance on transformation products. Despite the marginally aerobic conditions it is likely that highly reduced microzones existed in the soil particles during flooding and provided the opportunity for reductive dechlorination. In these experiments, planted microcosms with fully developed roots and rhizospheres showed significant reductive dechlorination and greater biotransformation than unplanted reactors. In addition, planted systems that were intermittently flooded had greater transformation of the parent PCB compounds than systems that were not. A poplar planted system resulted in the complete removal of 26 of the 29 PCB congeners detected in a commercial garden soil, while the unplanted soil only had 2 congeners completely removed after 96 days. In addition, the most recalcitrant congener, PCB 52, only decreased by 0.1% in the unplanted reactors while declining by 22.3% in the planted system. There was also greater removal of a PCB 77 spike in the planted system when compared to the unplanted system, 17.2% in the planted system versus 2.8% in the unplanted system. The results suggest that phytoremediation may be an effective tool in cleaning commercially available garden soils that are lightly contaminated with PCBs.

Densification of biomass is often necessary to combat the negative storage and handling characteristics of these low bulk density materials. A consistent, high-quality densified product is strongly desired, but not always delivered. Within the context of pelleting and briquetting, binding agents are commonly added to comminuted biomass feedstocks to improve the quality of the resulting pellets or briquettes. Many feedstocks naturally possess such binding agents; however, they may not be abundant enough or available in a form or state to significantly contribute to product binding. Also, process parameters (pressure and temperature) and material variables (particle size and moisture content) can be adjusted to improve the quality of the final densified product.<p>Densification of ground biomass materials is still not a science, as much work is still required to fully understand how the chemical composition and physical properties, along with the process variables, impact product quality. Generating densification and compression data, along with physical and mechanical properties of a variety of biomass materials will allow for a deeper understanding of the densification process. This in turn will result in the design of more efficient densification equipment, thus improving the feasibility of using biomass for chemical and energy production.<p>Experiments were carried out wherein process (pressure and temperature) and material (particle size and moisture content) variables were studied for their effect on the densification process (compression and relaxation characteristics) and the physical quality of the resulting products (pellets). Two feedstocks were selected for the investigation; namely, poplar wood and wheat straw, two prominent Canadian biomass resources. Steam explosion pretreatment was also investigated as a potential method of improving the densification characteristics and binding capacity of the two biomass feedstocks.<p> Compression/densification and relaxation testing was conducted in a closed-end cylindrical die at loads of 1000, 2000, 3000, and 4000 N (31.6, 63.2, 94.7, and 126.3 MPa) and die temperatures of 70 and 100°C. The raw poplar and wheat straw were first ground through a hammer mill fitted with 0.8 and 3.2 mm screens, while the particle size of the pretreated poplar and wheat straw was not adjusted. The four feedstocks (2 raw and 2 pretreated) were also conditioned to moisture contents of 9 and 15% wb prior to densification. <p> Previously developed empirical compression models fitted to the data elucidated that along with particle rearrangement and deformation, additional compression mechanisms were present during compression. Also, the compressibility and asymptotic modulus of the biomass grinds were increased by increasing the die temperature and decreasing product moisture content. While particle size did not have a significant effect on the compressibility, reducing it increased the resultant asymptotic modulus value. Steam explosion pretreatment served to decrease the compressibility and asymptotic modulus of the grinds.<p>In terms of physical quality of the resulting product, increasing the applied load naturally increased the initial density of the pellets (immediately after removal from the die). Increasing the die temperature served to increase the initial pellet density, decrease the dimensional (diametral and longitudinal) expansion (after 14 days), and increase the tensile strength of the pellets. Decreasing the raw feedstock particle size allowed for the increase in initial pellet density, decrease in diametral expansion (no effect on longitudinal expansion), and increase in tensile strength of the pellets. Decreasing the moisture content of the feedstocks allowed for higher initial pellet densities, but also an increased dimensional expansion. The pretreated feedstocks generally had higher initial pellet densities than the raw grinds. Also, the pretreated feedstocks shrank in diameter and length, and had higher tensile strengths than the raw feedstocks. The high performance of the pretreated poplar and wheat straw (as compared to their raw counterparts) was attributed to the disruption of the lignocellulosic structure, and removal/hydrolysis of hemicellulose, during the steam pretreatment process which was verified by chemical and Fourier transform infrared analysis. As a result, a higher relative amount of lignin was present. Also, the removal/hydrolysis of hemicellulose would indicate that this lignin was more readily available for binding, thus producing superior pellets.

The increasing input costs for traditional agriculture has led land owners and producers in search of alternative opportunities to increase on-farm income. Replacing agricultural crops with short rotation woody species such as hybrid poplar trees is a form of agroforestry. The objectives of this project were to evaluate: 1) a suitable planting stock for hybrid poplar, 2) the effect of nitrogen (N) fertilizer application and pruning on hybrid poplar growth and, 3) the response of four hybrid poplar clones to fertilizer application and their suitability in the boreal transition ecoregion of Saskatchewan. <p>Two trials were established near Meadow Lake, Saskatchewan where three stock types (cuttings, root cuttings and rooted plugs) of Walker poplar were planted into former alfalfa and pasture fields. Trees were pruned each spring to remove multiple leaders and fertilized in year 2 with 100 kg N ha-1. The presence of roots on rooted cutting and plug stock types was beneficial in terms of hybrid poplar growth and survival. Trees grown from planting stock without roots had survival rates between 32-37% whereas, the survival of trees with roots at the time of planting ranged from 62-81% after two years of growth. Trees that were planted as a rooted stock were 3.5 to 4.2 times greater in height and 4.0 to 5.6 times greater in root collar diameter than trees planted as an un-rooted stock type. The application of fertilizer N decreased tree volumes by 31% at the Alfalfa site and had no effect on tree growth at the Pasture site. The total amount of fertilizer N recovered by the hybrid poplar trees ranged from 1-3% at the Alfalfa site and 3-5% at the Pasture site. <p>The second study involved planting four clones of hybrid poplar (Hill, Katepwa, Walker and WP-69) at the same two sites and applying fertilizer at rates of 0, 150 and 300 kg N ha-1 the first two years. Following the second growing season, Katepwa and WP-69 clones had the highest tree volumes of 750 and 1147 cm3 of the four clones evaluated. The Walker clone had the poorest survival rates (52-56%) compared to the other three clones (> 90% survival). Foliar N levels were not correlated with tree height at the Alfalfa (p=0.1326) or the Pasture (p=0.1063) sites. The relationship between foliar P concentration and tree height was more pronounced during July at the Alfalfa site with an r2 value of 0.7102. The N:P ratios for foliar tissue decreased with increasing fertilizer N application during August at the Alfalfa site. Foliar N:P ratios were the same among fertilizer and clone treatments at the Pasture site in August. <p>Results from this study suggest that rooted stock types increase the successful establishment of hybrid poplar plantations. However, application of N fertilizer may not increase growth of trees if soil N is adequate. Other soil nutrients need to be measured prior to fertilization to determine what nutrients may be limiting plant growth.

Site quality, or the ability of land to grow trees, is an important component for identifying the most appropriate locations for establishing plantations of fast growing tree species to meet societys demands for timber and other environmental benefits. The goal of this thesis project was to predict site quality for poplars using soil and site information in Saskatchewan, Alberta and Quebec and to examine the effect of site quality on competition control in hybrid poplar plantations in Saskatchewan.<p>The first study examined factors affecting trembling aspen productivity in the boreal shield ecozone of Quebec on till and fluvial parent materials using general map data and measured soil and site information. Relationships with productivity were stronger using measured soil and site variables for individual parent materials (R2>0.6) than using general map data only (R2<0.25). Including biological variables, such as overstory species composition, had a major impact on site quality with conifer dominance negatively impacting the growth of trembling aspen.<p>The second study examined the factors affecting trembling aspen productivity in the boreal transition ecoregion of Saskatchewan on three different soil parent materials: fluvial, lacustrine and till. Relationships with productivity were stronger using soil and site variables for individual parent materials (R2 0.48-0.58) than using agricultural capability classes or other soil properties for all plots combined (R2<0.2). For fluvial and lacustrine sites, increasing clay content and nutrient availability (e.g. pH and total N) were positively related to productivity while tree productivity was negatively related to poor drainage for till sites. <p>The third study examined the factors affecting site quality for a single hybrid poplar clone in industrial plantations in Alberta at both the local scale (between plantations) and the microsite scale (within plantations). At the local scale, foliar P and Cu concentrations, soil water availability and drainage, and Ca and Mn in the C horizon were related to hybrid poplar productivity. There were also curved relationships with productivity and soil texture in the B horizon and pH of the A horizon, indicating an optimal range for poplar growth. At the microsite scale, soil texture was the best predictor of productivity with different relationships at each site depending on where the sites were in relation to the optimal soil texture.<p>The final study examined the response of hybrid poplar plantations in Saskatchewan to interspecific competition control on a range of site productivities. Competition control greatly increased tree growth with the greatest benefit being on the best quality sites. Both water and nutrients were highly competed for between trees and weeds. In the weed-free plots, tree growth was positively related to the amount of silt and clay in the soil and foliar P concentrations. This series of studies has demonstrated that it should be possible to predict poplar productivity reasonably well using only soil and site information within limited areas across Canada. However, the important drivers of productivity varied between the regions studied and between site groupings, such as by parent material, within local areas. This information can now be used to help land managers make better decisions regarding the establishment and management of plantations of fast growing tree species, notably hybrid poplar plantations.

Densification of biomass is often necessary to combat the negative storage and handling characteristics of these low bulk density materials. A consistent, high-quality densified product is strongly desired, but not always delivered. Within the context of pelleting and briquetting, binding agents are commonly added to comminuted biomass feedstocks to improve the quality of the resulting pellets or briquettes. Many feedstocks naturally possess such binding agents; however, they may not be abundant enough or available in a form or state to significantly contribute to product binding. Also, process parameters (pressure and temperature) and material variables (particle size and moisture content) can be adjusted to improve the quality of the final densified product.<p>Densification of ground biomass materials is still not a science, as much work is still required to fully understand how the chemical composition and physical properties, along with the process variables, impact product quality. Generating densification and compression data, along with physical and mechanical properties of a variety of biomass materials will allow for a deeper understanding of the densification process. This in turn will result in the design of more efficient densification equipment, thus improving the feasibility of using biomass for chemical and energy production.<p>Experiments were carried out wherein process (pressure and temperature) and material (particle size and moisture content) variables were studied for their effect on the densification process (compression and relaxation characteristics) and the physical quality of the resulting products (pellets). Two feedstocks were selected for the investigation; namely, poplar wood and wheat straw, two prominent Canadian biomass resources. Steam explosion pretreatment was also investigated as a potential method of improving the densification characteristics and binding capacity of the two biomass feedstocks.<p> Compression/densification and relaxation testing was conducted in a closed-end cylindrical die at loads of 1000, 2000, 3000, and 4000 N (31.6, 63.2, 94.7, and 126.3 MPa) and die temperatures of 70 and 100°C. The raw poplar and wheat straw were first ground through a hammer mill fitted with 0.8 and 3.2 mm screens, while the particle size of the pretreated poplar and wheat straw was not adjusted. The four feedstocks (2 raw and 2 pretreated) were also conditioned to moisture contents of 9 and 15% wb prior to densification. <p> Previously developed empirical compression models fitted to the data elucidated that along with particle rearrangement and deformation, additional compression mechanisms were present during compression. Also, the compressibility and asymptotic modulus of the biomass grinds were increased by increasing the die temperature and decreasing product moisture content. While particle size did not have a significant effect on the compressibility, reducing it increased the resultant asymptotic modulus value. Steam explosion pretreatment served to decrease the compressibility and asymptotic modulus of the grinds.<p>In terms of physical quality of the resulting product, increasing the applied load naturally increased the initial density of the pellets (immediately after removal from the die). Increasing the die temperature served to increase the initial pellet density, decrease the dimensional (diametral and longitudinal) expansion (after 14 days), and increase the tensile strength of the pellets. Decreasing the raw feedstock particle size allowed for the increase in initial pellet density, decrease in diametral expansion (no effect on longitudinal expansion), and increase in tensile strength of the pellets. Decreasing the moisture content of the feedstocks allowed for higher initial pellet densities, but also an increased dimensional expansion. The pretreated feedstocks generally had higher initial pellet densities than the raw grinds. Also, the pretreated feedstocks shrank in diameter and length, and had higher tensile strengths than the raw feedstocks. The high performance of the pretreated poplar and wheat straw (as compared to their raw counterparts) was attributed to the disruption of the lignocellulosic structure, and removal/hydrolysis of hemicellulose, during the steam pretreatment process which was verified by chemical and Fourier transform infrared analysis. As a result, a higher relative amount of lignin was present. Also, the removal/hydrolysis of hemicellulose would indicate that this lignin was more readily available for binding, thus producing superior pellets.

The increasing input costs for traditional agriculture has led land owners and producers in search of alternative opportunities to increase on-farm income. Replacing agricultural crops with short rotation woody species such as hybrid poplar trees is a form of agroforestry. The objectives of this project were to evaluate: 1) a suitable planting stock for hybrid poplar, 2) the effect of nitrogen (N) fertilizer application and pruning on hybrid poplar growth and, 3) the response of four hybrid poplar clones to fertilizer application and their suitability in the boreal transition ecoregion of Saskatchewan. <p>Two trials were established near Meadow Lake, Saskatchewan where three stock types (cuttings, root cuttings and rooted plugs) of Walker poplar were planted into former alfalfa and pasture fields. Trees were pruned each spring to remove multiple leaders and fertilized in year 2 with 100 kg N ha-1. The presence of roots on rooted cutting and plug stock types was beneficial in terms of hybrid poplar growth and survival. Trees grown from planting stock without roots had survival rates between 32-37% whereas, the survival of trees with roots at the time of planting ranged from 62-81% after two years of growth. Trees that were planted as a rooted stock were 3.5 to 4.2 times greater in height and 4.0 to 5.6 times greater in root collar diameter than trees planted as an un-rooted stock type. The application of fertilizer N decreased tree volumes by 31% at the Alfalfa site and had no effect on tree growth at the Pasture site. The total amount of fertilizer N recovered by the hybrid poplar trees ranged from 1-3% at the Alfalfa site and 3-5% at the Pasture site. <p>The second study involved planting four clones of hybrid poplar (Hill, Katepwa, Walker and WP-69) at the same two sites and applying fertilizer at rates of 0, 150 and 300 kg N ha-1 the first two years. Following the second growing season, Katepwa and WP-69 clones had the highest tree volumes of 750 and 1147 cm3 of the four clones evaluated. The Walker clone had the poorest survival rates (52-56%) compared to the other three clones (> 90% survival). Foliar N levels were not correlated with tree height at the Alfalfa (p=0.1326) or the Pasture (p=0.1063) sites. The relationship between foliar P concentration and tree height was more pronounced during July at the Alfalfa site with an r2 value of 0.7102. The N:P ratios for foliar tissue decreased with increasing fertilizer N application during August at the Alfalfa site. Foliar N:P ratios were the same among fertilizer and clone treatments at the Pasture site in August. <p>Results from this study suggest that rooted stock types increase the successful establishment of hybrid poplar plantations. However, application of N fertilizer may not increase growth of trees if soil N is adequate. Other soil nutrients need to be measured prior to fertilization to determine what nutrients may be limiting plant growth.

Site quality, or the ability of land to grow trees, is an important component for identifying the most appropriate locations for establishing plantations of fast growing tree species to meet societys demands for timber and other environmental benefits. The goal of this thesis project was to predict site quality for poplars using soil and site information in Saskatchewan, Alberta and Quebec and to examine the effect of site quality on competition control in hybrid poplar plantations in Saskatchewan.<p>The first study examined factors affecting trembling aspen productivity in the boreal shield ecozone of Quebec on till and fluvial parent materials using general map data and measured soil and site information. Relationships with productivity were stronger using measured soil and site variables for individual parent materials (R2>0.6) than using general map data only (R2<0.25). Including biological variables, such as overstory species composition, had a major impact on site quality with conifer dominance negatively impacting the growth of trembling aspen.<p>The second study examined the factors affecting trembling aspen productivity in the boreal transition ecoregion of Saskatchewan on three different soil parent materials: fluvial, lacustrine and till. Relationships with productivity were stronger using soil and site variables for individual parent materials (R2 0.48-0.58) than using agricultural capability classes or other soil properties for all plots combined (R2<0.2). For fluvial and lacustrine sites, increasing clay content and nutrient availability (e.g. pH and total N) were positively related to productivity while tree productivity was negatively related to poor drainage for till sites. <p>The third study examined the factors affecting site quality for a single hybrid poplar clone in industrial plantations in Alberta at both the local scale (between plantations) and the microsite scale (within plantations). At the local scale, foliar P and Cu concentrations, soil water availability and drainage, and Ca and Mn in the C horizon were related to hybrid poplar productivity. There were also curved relationships with productivity and soil texture in the B horizon and pH of the A horizon, indicating an optimal range for poplar growth. At the microsite scale, soil texture was the best predictor of productivity with different relationships at each site depending on where the sites were in relation to the optimal soil texture.<p>The final study examined the response of hybrid poplar plantations in Saskatchewan to interspecific competition control on a range of site productivities. Competition control greatly increased tree growth with the greatest benefit being on the best quality sites. Both water and nutrients were highly competed for between trees and weeds. In the weed-free plots, tree growth was positively related to the amount of silt and clay in the soil and foliar P concentrations. This series of studies has demonstrated that it should be possible to predict poplar productivity reasonably well using only soil and site information within limited areas across Canada. However, the important drivers of productivity varied between the regions studied and between site groupings, such as by parent material, within local areas. This information can now be used to help land managers make better decisions regarding the establishment and management of plantations of fast growing tree species, notably hybrid poplar plantations.

No description available.