Master of Science / Biology / David A. Haukos / The lesser prairie-chicken (Tympanuchus pallidicinctus) has experienced range-wide population declines and range contraction since European settlement. Due to ongoing declines, lesser prairie-chickens were listed as threatened under the Endangered Species Act in 2014; however, uncertainty regarding the legal status of the species has developed following a judicial decision to vacate the listing in September 2015. Regardless, new research is required for conservation planning, especially for understudied portions and temporal periods of the occupied range. I evaluated nonbreeding lesser prairie-chicken survival using known-fate models, and tested for the influence of environmental, landscape and predator effects on weekly survival. I estimated nonbreeding home-range size using fixed kernel density estimators and Brownian Bridge movement models for VHF and Satellite tagged lesser prairie-chickens, and measured habitat use during the 6-month nonbreeding period (16 September – 14 March). I also determined the influence of lek location on space use intensity within home ranges using resource utilization functions. Female survival was high (0.75, SE = 0.05) and consistent across nonbreeding seasons, but not explainable by selected variables. Mean home range size for birds with GPS transmitters (955 ha, SE = 128.5) was 215% larger than for individuals with VHF transmitters (303 ha, SE = 24.1) and 136% greater during the 2014-2015 nonbreeding season than the 2013-2014 season. Males and females were tied to leks throughout the nonbreeding season, and this relationship was not variable across the months of the nonbreeding season. Proportions of habitat used differed among study sites, but temporal trends were not evident. Lesser prairie-chickens exhibited consistency among ecoregions for home-range, space use, and survival; however, with differing habitat use among regions, management should be on the regional scale. Agriculture and energy development have caused fragmentation of the landscape where lesser prairie-chickens evolved. I used known fate survival models to test if landscape composition or configuration within sites caused survival to differ by site, as well as within home ranges to determine if functional relationships exist between weekly survival and landscape configuration or composition. I used Andersen-Gill models to test whether distance to anthropogenic features affected hazard rates. Differences in survival rates between sites, with survival rates 50% greater in Clark County, Kansas compared to Northwestern, Kansas, corresponded to differences in the amount of grassland habitat on the landscape, but study-site configuration was not measurably different. Increasing the number of patch types within home ranges increased survival, indicating positive effects of heterogeneity. In addition, as distance to fences decreased, lesser prairie-chickens experienced greater risk. Overall, further breakup of grassland landscapes that lesser prairie-chickens occupy should be avoided, to avoid habitat loss and fragmentation thresholds that could further affect survival rates. Additionally, fences should be removed or avoided around active leks.

Master of Science / Department of Geography / Melinda Daniels / The Lesser Prairie-Chicken (Tympanuchus pallidicinctus) is a prairie grouse of conservation concern in the Southern Great Plains. In response to declining population numbers and ongoing threats to its habitat, the Lesser Prairie-Chicken was listed as threatened under the Endangered Species Act in May 2014. In western Kansas, the Lesser Prairie-Chicken occupies the Sand Sagebrush Prairie, Mixed-grass Prairie, and Short-grass/CRP Mosaic Ecoregions. Since the beginning of the 20th century, the overall range and population has declined by 92% and 97% respectively. Much of this decline is attributed to the loss and fragmentation of native grasslands throughout the Lesser Prairie-Chicken range. Whereas much of the loss and degradation of native grassland have been attributed to anthropogenic activities such as conversion of grassland to cropland and energy exploration, federal legislation since the 1980s to convert cropland on highly erodible soils to perennial grasses through the U.S. Department of Agriculture (USDA) Conservation Reserve Program (CRP) may curtail or reverse these trends. My objective was to document changes in the areal extent and connectivity of grasslands in the identified Lesser Prairie-Chicken range in Kansas from the 1950s to 2013 using remotely sensed data. I hypothesized that the total amount of grassland decreased between the 1950’s and 2013 because of an increase in agricultural practices, but predicted an increase of grassland between 1985 and 2013 in response to the CRP. To document changes in grassland, land cover maps were generated through spectral classification of LANDSAT images and visual analysis of aerial photographs from the Army Map Service and USDA Farm Service Agency. Landscape composition and configuration were assessed using FRAGSTATS to compute a variety of landscape metrics measuring changes in the amount of grassland present as well as changes in the size and configuration of grassland patches. Since 1985, the amount of grassland in the Lesser Prairie-Chicken range in Kansas has increased by 210,9963.3 ha, a rise of 11.9%, while the mean patch size and area-weighted mean patch size of grassland increased 18.2% and 23.0% respectively, indicating grassland has become more connected during this time in response to the CRP. Prior to the implementation of CRP, the amount of grassland had been decreasing since 1950, as 66,722.0 ha of grassland was converted to croplands. The loss of grassland had a considerable effect on the patch size of grasslands, as mean patch size and area-weighted mean patch size decreased by 8.8% and 11.1% respectively. The primary driver of grassland loss between 1950 and 1985 was the emergence of center pivot irrigation, which had its greatest impact in western and southwestern parts of the range in Kansas. In particular, while the amount of grassland in Range 5, a region of the Lesser Prairie-Chicken range found in southwest Kansas, has increased overall since the 1950s by 4.7%, the area-weighted mean patch size has decreased by 53.0% in response to center pivot irrigation fragmenting the landscape. While the CRP has been successful in increasing and connecting grassland throughout the Lesser Prairie-Chicken range to offset the loss of grassland since the 1950s, continuation of the CRP faces an uncertain future in the face of rising commodity prices, energy development, and reduction in program scope leaving open the possibility that these areas that have created habitat for Lesser Prairie-Chickens could be lost. As time progresses, a reduction in the scope of the CRP would reduce the amount of habitat available to Lesser Prairie-Chickens, threatening the persistence of their population.

Master of Science / Department of Biology / David A. Haukos / The lesser prairie-chicken (Tympanuchus pallidicinctus) is a species of prairie grouse native to the southwest Great Plains. Population declines and threats to populations of lesser prairie-chickens led U.S. Fish and Wildlife Service to list the species as “threatened” under the protection of the Endangered Species Act in May 2014. Lesser prairie-chickens are found within three distinct ecoregions of Kansas and Colorado and portions of the species’ range are affected by tree encroachment into grasslands. The effect of trees on lesser prairie-chickens is poorly understood. I evaluated habitat selection and reproductive success and across the northern portion of the species’ range. I captured female lesser prairie-chickens within the three different ecoregions in Kansas and Colorado to track nest and brood survival and measure nest and brood habitat. My findings show that there are regional and annual variations in nest and brood survival. Mean nest survival during 2013 and 2014 was estimated to be 0.388 (95% CI = 0.343 – 0.433) for a 35-day exposure period. Brood survival during 2013 and 2014 was estimated to be 0.316 (95% CI = 0.184 – 0.457) for 56 days. Chick survival was the lowest during the first week of life and is probably a limiting factor for population growth. Chick and brood survival decreased as Julian hatch date increased. Across the northern portion of the species’ range, females consistently select visual obstruction between 2-3 dm. Vegetation at the nest changes between regions and years to reflect environmental and regional conditions. Broods consistently selected habitats with greater percent cover of forbs than was expected at random across all study sites. Broods also selected against areas of bare ground. The threshold of lesser prairie-chicken use was 2 trees/ha throughout the year. No nests were located within areas with greater densities. Lesser prairie-chickens had a greater probability of use at greater distances from trees and at lower tree densities. To provide adequate nesting habitat managers should provide 2-3 dm of visual obstruction. Providing forb cover with visual obstruction between 2.5-5 dm near nesting habitat should provide adequate habitat for broods. Removing trees in core habitats and expand removal efforts outward should expand potential habitat for lesser prairie-chickens.

<p>Greater prairie-chickens (<i>Tympanuchus cupido pinnatus</i>; GPC) have declined throughout large areas in the eastern portion of their range. I used species distribution modeling to predict most appropriate areas of translocation of GPC in and around Kankakee Sands, a tallgrass prairie in northwest Indiana, USA. I used MaxEnt for modelling the predictions based on relevant environmental predictors along with occurrence points of 54 known lek sites. I created four models inspired by Hovick et al. (2015): Universal, Environmental, Anthropogenic-Landcover, and Anthropogenic-MODIS. The Universal, Environmental, and Anthropogenic-MODIS models possessed passable AUC scores with low omission error rates. However, only the Universal model performed better than the null model according to binomial testing. I created maps of all models with passing AUC scores along with an overlay map displaying the highest predictions across all passing models. MaxEnt predicted high relative likelihoods of occurrence for the entirety of Kankakee Sands and many areas in the nearby landscape, including the surrounding agricultural matrix. With implementation of some management suggestions and potential cooperation with local farmers, GPC translocation to the area appears plausible.</p> <p>Franklin’s ground squirrels (<i>Poliocitellus franklinii</i>; FGS) have declined throughout a large portion of the eastern periphery of their range. Because of this, The Nature Conservancy is interested in establishing a new population of these animals via translocation. The area of interest is tallgrass prairie in northwest Indiana, USA: Kankakee Sands and the surrounding landscape. Species distribution modelling can help identify areas that are suitable for translocation. I used MaxEnt, relevant environmental variables, and 44 known occurrence points to model the potential for translocation of FGS to Kankakee Sands and the surrounding area. I created four models inspired by Hovick et al. (2015): Universal, Environmental, Anthropogenic-Landcover, and Anthropogenic-MODIS. I created maps of models with passing AUC scores. The final map was an overlay map displaying the highest relative likelihood of occurrence predictions for the area in all passing models. Only the Universal and Anthropogenic-MODIS models had passable AUC scores. Both had acceptable omission error rates. However, none of the models performed better than the null model (p < 0.05). MaxEnt predicted that a few areas in and outside of Kankakee Sands possess high relative likelihoods of occurrence of FGS in both the Universal and Anthropogenic-MODIS models. However, MaxEnt predicted high relative likelihoods in the surrounding agricultural matrix in the Universal Model. FGS prefer to cross through agricultural areas via unmowed roadside instead of open fields (Duggan et al. 2011). Because of this, high predictions in agricultural matrices in the Universal model are irrelevant. High relative likelihood predictions for linear sections that are obviously roads are disregardable in the context of my modeling efforts. Because of my low sample size, none of the models are really reliable in predicting relative likelihoods of occurrence for this area. Despite high relative likelihood predictions, the appropriateness of a translocation effort to the area is inconclusive.</p>

No description available.

Unambiguous gender identification (ID) is needed to assess parameters in studies of population dynamics, behavior, and evolutionary biology of Caribbean Flamingo (Phoenicopterus ruber ruber) and other birds. Due to its importance for management and conservation, molecular (DNA-based) avian gender ID assays targeting intron-size differences of the Chromosome Helicase ATPase DNA Binding (CHD) gene of males (CHD-Z) and females (CHD-W) have been developed. Male (ZZ) and female (WZ) genotypes are usually scored as size polymorphisms through agarose or acrylamide gels. For certain species, W-specific restriction sites or multiplex polymerase chain-reaction (PCR) involving CHD-W specific primers are needed. These approaches involve a minimum of three steps following DNA isolation: PCR, gel electrophoresis, and photo-documentation, which limit high throughput scoring and automation potential. In here, a short amplicon (SA) High-resolution Melting Analysis (HRMA) assay for avian gender ID is developed. SA-HRMA of an 81-Base Pair (bp) segment differentiates heteroduplex female (WZ) from homoduplex male (ZZ) genotypes by targeting Single-nucleotide Polymorphisms (SNPs) instead of intron-size differences between CHD-Z and CHD-W genes. To demonstrate the utility of the approach, the gender of Caribbean Flamingo (P. ruber ruber) (17 captive from the Dallas Zoo and 359 wild from Ria Lagartos, Yucatan, Mexico) was determined. The assay was also tested on specimens of Lesser Flamingo (P. minor), Chilean Flamingo (P. chilensis), Saddle-billed Stork (Ephippiorhynchus senegalensis), Scarlet Ibis (Eudocimus ruber), White-bellied Stork (Ciconia abdimii), Roseate Spoonbill (Platalea ajaja), Marabou Stork (Leptoptilos crumeniferus), Greater Roadrunner (Geococcyx californianus), and Attwater's Prairie Chicken (Tympanuchus cupido attwateri). Although the orthologous 81 bp segments of Z and W are highly conserved, sequence alignments with 50 avian species across 15 families revealed mismatches affecting one or more nucleotides within the SA-HRMA forward or reverse primers. Most mismatches were located along the CHD-Z gene that may generate heteroduplex curves and thus gender ID errors. For such cases, taxon and species-specific primer sets were designed. The SA-HRMA gender ID assay can be used in studies of avian ecology and behavior, to assess sex-associated demographics and migratory patterns, and as a proxy to determine the health of the flock and the degree by which conservation and captive breeding programs are functioning.