Thesis (Ph. D.)--Oregon State University, 1974. / Typescript (photocopy). Includes bibliographical references. Also available on the World Wide Web.

Thesis (M.S.)--Oregon State University, 2007. / Printout. Includes bibliographical references (leaves 113-116). Also available on the World Wide Web.

The Chinese herb Artemisia annua possesses small 10-cell biseriate glandular trichomes on the surface of its aerial tissues. These trichomes were isolated from floral tissue using a Bead Beater based method. Expression patterns of expressed sequence tags from a trichome library whose Basic Local Alignment Search Tool (BLAST) results suggested a possible function in terpenoid metabolism were investigated by RT-PCR. Known terpenoid biosynthetic enzymes, such as amorpha-4,11-diene synthase showed a high degree of trichome-specific expression. In order to investigate cell specific gene expression within the trichome, the promoter for the gene encoding amorpha-4,11-diene synthase was isolated but the lack of an efficient transformation protocol in A. annua hindered reporter gene localization experiments. Traditional and whole-mount <i>in situ</i> hybridization techniques were used to further the study of cell specific gene expression within the glandular trichome. An RNA probe constructed from the sequence of amorpha-4,11-diene synthase localized expression to the 2nd and 3rd subapical cell pairs of the glandular trichomes. This suggests that at least part of the artemisinin biosynthetic pathway resides within the lower cell pairs. To better understand the genes involved in terpenoid biosynthesis in A. annua, the full length sequence of a short chain alcohol dehydrogenase highly represented in an expressed sequence tag library and shown to have trichome-specific expression by RT-PCR, was cloned. Heterologous expression in Escherichia coli demonstrated that the enzyme was capable of oxidizing a wide range of monoterpenols to their corresponding ketone forms. All of this data helps us to better understand the organization of expression and biochemistry of terpenoids in A. annua glandular trichomes.

The Chinese herb Artemisia annua possesses small 10-cell biseriate glandular trichomes on the surface of its aerial tissues. These trichomes were isolated from floral tissue using a Bead Beater based method. Expression patterns of expressed sequence tags from a trichome library whose Basic Local Alignment Search Tool (BLAST) results suggested a possible function in terpenoid metabolism were investigated by RT-PCR. Known terpenoid biosynthetic enzymes, such as amorpha-4,11-diene synthase showed a high degree of trichome-specific expression. In order to investigate cell specific gene expression within the trichome, the promoter for the gene encoding amorpha-4,11-diene synthase was isolated but the lack of an efficient transformation protocol in A. annua hindered reporter gene localization experiments. Traditional and whole-mount <i>in situ</i> hybridization techniques were used to further the study of cell specific gene expression within the glandular trichome. An RNA probe constructed from the sequence of amorpha-4,11-diene synthase localized expression to the 2nd and 3rd subapical cell pairs of the glandular trichomes. This suggests that at least part of the artemisinin biosynthetic pathway resides within the lower cell pairs. To better understand the genes involved in terpenoid biosynthesis in A. annua, the full length sequence of a short chain alcohol dehydrogenase highly represented in an expressed sequence tag library and shown to have trichome-specific expression by RT-PCR, was cloned. Heterologous expression in Escherichia coli demonstrated that the enzyme was capable of oxidizing a wide range of monoterpenols to their corresponding ketone forms. All of this data helps us to better understand the organization of expression and biochemistry of terpenoids in A. annua glandular trichomes.

Marine natural products have continued to be a source of compounds with interesting structures and biological activities. Two such compounds are maculalactone A and haterumalide NA. In the process of exploring a route to the synthesis of haterumalide NA, the novel ring system, spiroepoxy-b-lactones were discovered. Spiroepoxy-b-lactones were synthesized by the oxidation of ketene-homo dimers with dimethyldioxirane (DMDO). After a synthetic route to this ring system was obtained we next explored the varied reactivity of spiroepoxy-b-lactones, and it was apparent that they might be applied to the synthesis of maculalactone A. Also with the aim of the total synthesis of the haterumalides, a palladium catalyzed cross coupling was developed. This reaction couples a 1,1-dichloroolefin with an alkyl zinc reagent. It was found that this reaction necessitates a heteroatom on the zinc reagent in order to proceed.

Corn milling (co)products (n=120) were evaluated for their neutral detergent fiber residue (NDR) and neutral detergent insoluble protein (NDIP) ruminal degradation rates using several in vitro methods. Two (co)products (BPX-DDGS and HP-DDG) were fed to lactating dairy cows (n=44) to evaluate effects on milk production. The Cornell-Penn-Miner Institute (CPM) Dairy model was used to formulate diets and predict milk production. In vitro determined NDR and NDIP rates and were compared to CPM-dairy feed library values, and model predictions were compared with observed milk production. Additionally, BPX-DDGS and HP-DDG were defatted and compared with their intact forms for fermentation characteristics using the in vitro gas production (IVGP) technique. Fermentations were analyzed for rumen bacterial population shifts using the 16S rDNA bacterial tag encoded FLX amplicon pyrosequencing (bTEFAP) technique. Lastly, a novel ruminal in vitro method was described to measure the soluble protein fraction of feeds, with adjustments for microbial contamination. Fermentation rate of the NDR of BPX-DDGS and HP-DDG (0.08 and 0.07 h^-1, respectively) and NDIP degradation rates (0.07 and 0.06 h^-1, respectively) were similar to CPM-dairy feed library NDR and NDIP rates of corn distillers grain (0.07 and 0.05 h^-1, respectively). Model predictions using standard and in vitro determined values did not differ. As BPX-DDGS decreased and HP-DDG increased in the diet, observed milk production tended to decrease linearly (P = 0.08). There was a cubic effect for milk fat percent (P = 0.03) and a cubic trend for milk fat yield (P = 0.09). Milk protein yield also tended to decrease linearly (P = 0.06). CPM-dairy model prediction accuracies were less than 50 percent. Defatting (co)products reduced lag time and fractional rate of fermentation by at least half for BPX-DDG, and had no effect on HP-DDG. Defatting both (co)products increased the fibrolytic (26.8 to 38.7 percent) and proteolytic (26.1 to 37.2 percent) bacterial guild populations and decreased the lactate-utilizing bacterial guild (3.06 to 1.44 percent). The novel ruminal in vitro method determined that the specific activity of ammonia production was not different among (co)products. However, results were within numerical range of previously used methodologies.

Diss.--Technische Universität München, 2009. / Includes bibliographical referendes.

Thesis (M.S. in food science)--Washington State University, December 2009. / Title from PDF title page (viewed on Jan. 4, 2010). "School of Food Science." Includes bibliographical references (p. 129-143).

Inaug.-Diss.--Marburg. / Lebenslauf. "Separat-Abdruck aus den Zoologischen Jahrbüchern, Bd. 22, Abt. f. Anatomie." Literaturverzeichnis": p. 39-40.

Thesis (Ph. D.)--University of Chicago, Dept. of Economics, 2001. / Includes bibliographical references. Also available on the Internet.