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Vibrational microscopy for super-multiplexing, vibrational sensing and high-throughput metabolic imagingShi, Lixue January 2020 (has links)
Vibrational imaging approaches including Raman microscopy and IR-absorption micro-spectroscopy can provide rich chemical information about biological samples. This dissertation contributes to improve the capabilities of vibrational microscopy in three aspects each with corresponding biological applications. First, along the line of combining electronic resonant effect with stimulated Raman scattering (SRS), we studied the spectroscopic characteristics for on-resonant SRS case and demonstrated the utility of electronic pre-resonant SRS on super-multiplexed imaging for live cells and tissue sections. Second, we provided a new light-matter interaction as a hybrid technique of Raman and fluorescence, called stimulated Raman excited fluorescence (SREF), bringing the long-sought-after goal of detecting single-molecule Raman scattering without plasmonic enhancement into view. Coupling SREF with vibrational sensing, local electric field and hydrogen-bonding environment can thus be visualized in situ. Third, we brought small vibrational probes into mid-infrared imaging for the goal of rich-information-content, high-throughput metabolic imaging.
Chapter 1 introduces some basis of Raman scattering, and provides an overview of state-of-art SRS microscopy.
Chapter 2 explores on the rigorous electronic resonant region with SRS (er-SRS) through suppression of electronic background and subsequent retrieval of vibrational peaks. In agreement with theoretical prediction, changing of vibrational band shapes from normal Lorentzian, through dispersive shapes, to inverted Lorentzian is observed when approaching electronic resonance. As large as 10-23 cm2 of resonance Raman cross section is estimated in er-SRS.
In Chapter 3, a new light-matter interaction called stimulated Raman excited fluorescence (SREF) is studied. Through stimulated Raman pumping to an intermediate vibrational eigenstate followed by an upconversion to an electronic fluorescent state, SREF encodes vibrational resonance into the excitation spectrum of fluorescence emission. By leveraging superb sensitivity of SREF, we achieved all-far-field single-molecule Raman spectroscopy and imaging without plasmonic enhancement.
Chapter 4 details the development of SREF into a novel water-sensing tool, by coupling with vibrational solvatochromism of environment-sensitive Raman mode. This new technique allows direct visualization on spatially-resolved distribution of water states inside single mammalian cells. Interesting intracellular heterogeneity of water states between nucleus and cytoplasm has been revealed.
Chapter 5 demonstrates the utility of epr-SRS in super-multiplexed imaging with either commercial fluorophores in lives cells or our MARS probes on tissue sections. Multiplex protein-based tissue imaging is completed with newly-designed functional MARS dye with up to 12 channels simultaneously.
Chapter 6 focus on metabolic imaging by mid-infrared (MIR) microscopy with vibrational probes. Raman scattering microscopy has made a major advance in metabolic imaging utilizing vibrational probes, yet is limited to relatively low throughput. As an alternative solution, MIR microscopy provides significantly higher cross section and exhibits as a rich-information-content, high-throughput technique with recent rapid technical advances. We introduced three types of small vibrational probes as azide, 13C and carbon-deuterium for studying dynamic metabolic activities of protein, lipids and carbohydrates in cells, small organisms and mice for the first time. Two MIR microscopy platforms as Fourier transform infrared (FTIR) absorption microscopy and discrete frequency mid-infrared (DFIR) microscopy were utilized to validate the generality of our vibrational probes and applicability for single-cell metabolic profiling and metabolism study on large-scale tissues.
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A Study of Scientific Reasoning in a Peripheral Context: The Discovery of the Raman EffectDasgupta, Deepanwita 01 January 2015 (has links)
This paper is an attempt to reconstruct how C.V. Raman, a peripheral scientist in the early 20th century colonial India, managed to develop a research programme in physical optics from his remote colonial location. His attempts at self-training and self-education eventually led him to the discovery of the Raman Effect and to the Nobel Prize in Physics in 1930. In trying to re-construct an account of the ways in which Raman developed his research programme in optics by grasping various elements of a scientific practice from the Western scientific community, we see how a newcomer in science could be surprisingly creative in achieving new breakthroughs, and how through such efforts he or she can establish new trading zones with another established community that has the potential to develop into independent practices.
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Infrared spectrum of solid D̳2 and Raman spectra of solid H̳2, D̳2 and HD /Baliga, Shankar B. January 1986 (has links)
No description available.
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Raman studies of the nanostructure of sol-gel materialsDoss, Calvin James 06 June 2008 (has links)
Four sol-gel systems (alumina, aluminum hydroxide, zirconia, and magnesia) were investigated, primarily by laser spectroscopy, on several series of materials prepared by systematically varying the synthesis procedures. Key material phases analyzed were: (1) nanocrystalline boehmite [AlO(OH)]; (2) bayerite, nordstrandite, and gibbsite [Al(OH)₃ polymorphs]; (3) amorphous zirconia [a-ZrO₂]; and (4) magnesite [MgCO₃], hydromagnesite [4MgCO₃·Mg(OH)₂·5H₂0], and magnesia [MgO]. Crystal nucleation and crystal growth kinetics were studied in several cases, with x-ray experiments carried out to calibrate the Raman-scattering technique I developed for monitoring crystallite size.
Nanocrystalline boehmite, γ-AlO(OH), was found to be the principal component in the sol-gel alumina system. Materials were prepared by the hot-water hydrolysis/condensation of Al(OC₄H₉)₃, the Yoldas process, as a function of process variables such as the time spent in the sol phase. Small but systematic changes, as a function of sol aging time, were discovered in the lineshape and position of the dominant boehmite Raman band observed in the alumina hydrogels. These spectral changes were interpreted in terms of nanocrystallinity-induced finite-size effects associated with the slow growth of AlO(OH) nanocrystals in the sol. X-ray diffraction experiments were used to determine nanocrystal sizes (as small as 3 nm for gels prepared from fresh sols) and to estimate growth kinetics from the Raman-lineshape results. These results appear to be among the first available for crystallite growth kinetics (ripening) in the near-atomic-scale nanocrystal regime. The Raman peak-position shift is proportional to L<sup>-α</sup>, where L is the average nanocrystal size and α is a Raman-versus-size scaling exponent. For AlO(OH), I determined α to be 1.0, close to the scaling-exponent values reported for graphite and BN and different from the values (about 1.5) that describe the reported behavior of Si, Ge, and GaAs.
The trihydroxide polymorph system is closely related to the sol-gel alumina system. The processing temperature and the method of hydrolysis were varied, in order to determine their effect on the trihydroxide phase mix. The trihydroxide phase mix does not change with time; it depends only on the initial hydrolysis conditions. Bayerite is the primary phase present for materials processed at 25 C, while nordstrandite is the primary phase present for materials processed at 60 C. It is shown that the trihydroxide crystal nucleation kinetics are responsible for the Al(OH)₃ phase mix. Hydroxide/oxyhydroxide phase-mix kinetics were also studied; this ratio increases with time. The associated rate constant decreases with increasing temperature.
Sol-gel zirconia was prepared by using atmospheric water to hydrolyze a mixture of zirconium propoxide, acetic acid, and n-propanol. This produces a clear gel. Hydrogen peroxide was found to chemically react with the gels. Clean Raman spectra reveal a broad-band structure (the full width at half maximum is 150 cm⁻¹) centered at about 460 cm⁻¹. This band is interpreted as the signature of an amorphous phase of zirconia.
Raman and luminescent spectra (both obtained on the Raman spectrometer) were used to monitor the conversion of magnesium-carbonate-based materials to magnesium oxide, as a function of temperature. This new phase-determination technique utilizes the krypton 674.1 nm laser line so that the carbonate symmetric-stretch band and the MgO:Cr⁺⁺⁺ luminescence band are readily observable on the same spectrum. / Ph. D.
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Bolaamphiphile nanotubes : from gene delivery to nano-electronicsGologan, Bogdan 01 July 2000 (has links)
No description available.
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A CHARACTERIZATION OF THE OXIDATION-REDUCTION CYCLE AND SURFACE MORPHOLOGY OF ELECTROCHEMICAL SURFACE ENHANCED RAMAN SCATTERINGTuschel, David Daniel, 1957- January 1986 (has links)
No description available.
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A characterization of beam shaping devices and a tunable Raman laserDu Plessis, Anton 04 1900 (has links)
Thesis (MSc)--University of Stellenbosch, 2003. / ENGLISH ABSTRACT: The efficient manipulation of various nonlinear optical processes frequently requires the
shaping of the laser beams used for these processes. Three beam shaping techniques were investigated
in this thesis. The focussing of Gaussian laser beams was investigated analytically, in
order to efficiently manipulate the focussed beam characteristics. The beam-shaping characteristics
of a diffractive optical element (DOE) was investigated numerically, which illustrates the beamshaping
capability of the DOE, and identifies the critical parameters in experimental situations.
The use of a waveguide as beam shaping device was investigated analytically and experimentally,
and characterized for use with the available tunable laser sources. A Raman laser, or Raman shifter,
employs stimulated vibrational Raman scattering to generate laser radiation at shifted frequencies.
The waveguide was successfully applied as a beam shaping device in the Raman laser system, for
optimisation of the process. The Raman laser system was investigated experimentally and characterized
for use with the available tunable laser sources. The successful generation of laser radiation
at shifted frequencies illustrates the usefulness of the system for generating tunable red-shifted frequencies.
The results of this work allow the simple and efficient application of the Raman laser to
generate laser radiation at shifted frequencies, in particular tunable infrared laser radiation which
is desirable for molecular spectroscopy. / AFRIKAANSE OPSOMMING: Nie-liniêre optiese prosesse kan meer effektief benut word deur die vervorming van die laserbundels
wat gebruik word in die prosesse. In hierdie tesis word drie laserbundel-vervormings tegnieke
ondersoek. Die fokussering van Gaussiese laserbundels word analities ondersoek, om die
gefokusseerde bundel se eienskappe effektief te manipuleer. Die bundel-vervormings eienskappe
van ’n diffraktiewe optiese element word numeries ondersoek, wat die effektiwiteit van die bundelvervorming
en die sensitiewe parameters in die sisteem uitwys. Die gebruik van ’n golfgeleier as ’n
bundel-vervormings tegniek word ook analities en eksperimenteel ondersoek, en gekarakteriseer
vir gebruik met die gegewe golflengte-verstelbare laser sisteme. ’n Raman laser, wat gestimuleerde
vibrasionele Raman verstrooiing gebruik om laser lig te genereer by Stokes-verskuifde frekwensies,
word ondersoek. Die golfgeleier word effektief gebruik as ’n bundel-vervormings tegniek in
die Raman laser, om die bogenoemde nie-liniêre proses te optimeer. Die Raman laser was eksperimenteel
ondersoek en gekarakteriseer vir gebruik met die gegewe golflengte-verstelbare lasers.
Laser lig by verskuifde golflengtes is suksesvol gegenereer, wat die bruikbaarheid van die sisteem
illustreer. Van belang is spesifiek verstelbare infrarooi laser lig, wat gebruik kan word in die
laser-spektroskopie van molekules. Die resultate van hierdie werk lei tot die eenvoudige en effektiewe
gebruik van die Raman laser, om langer golflengtes in die infrarooi gebied te genereer met
’n gegewe laser in die sigbare gebied.
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Time-resolved resonance Raman investigation of selected para-substituted phenylnitrenium ions and the 2-fluorenylnitrenium ionreaction with guanosineChan, Pik-ying., 陳碧瑩. January 2005 (has links)
published_or_final_version / abstract / Chemistry / Doctoral / Doctor of Philosophy
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Time-resolved resonance raman and density functional theory studies ofselected arylnitrenium ions and their reactions with guanosinederivatives and aryl azidesXue, Jiadan., 薛佳丹. January 2008 (has links)
published_or_final_version / Chemistry / Doctoral / Doctor of Philosophy
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Development of new polymer-supported reagents for organic synthesis, solvent effects in samarium promoted allylic alcohol cyclopropanationreactions and time resolved resonance studies of the photodeprotectionof p-hydroxyphenacyl caged phototrigger compoundsKan, Tze-wai, Jovi., 簡紫慧. January 2006 (has links)
published_or_final_version / abstract / Chemistry / Doctoral / Doctor of Philosophy
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