Particle accelerators are machines to accelerate and store charged particle beams, such as electrons or protons, to the energy levels for various scientific applications. There are three basic types of particle accelerators: linear accelerators (linac), storage-ring (or circular) accelerators, and recirculating accelerators. The third type, also the most recent one, is designed to accelerate a particle beam in a short section of linac, circulate and then continue to accelerate it for energy boost or decelerate it for energy recovery. The modern recirculating machines possess the advantages to both accelerate and preserve the beam with high beam quality, as well as efficiently reuse the accelerating components. As modern accelerators push toward the high-brightness or high-intensity frontier by demanding particles in a highly charged bunch to concentrate in an ever-decreasing beam phase space, the interaction amongst particles via their self-generated electromagnetic fields can potentially lead to coherent instabilities of the beam and thus pose significant challenges to the machine design and operation. Microbunching instability (MBI) has been one of the most challenging issues for such high-brightness or high-intensity beam transport, as it would degrade lasing performance in the fourth-generation light sources, reduce cooling efficiency in electron cooling facilities, and eventually compromise the luminosity of colliding beams in lepton or lepton-hadron colliders. The dissertation work will focus on the MBI in modern recirculating electron accelerators. The research attempts to develop a comprehensive theoretical formulation of MBI with aspects including among various degrees of freedoms the beam itself, the beamline lattice optics, and incorporation of all relevant collective effects that the beam encounters, for example the coherent synchrotron radiation (CSR) and the longitudinal space charge (LSC) effects. This dissertation includes the following seven themes: 1) Development and generalization of MBI theory to arbitrary linear lattices and coupled beams with constant and varying energies; 2) Construction of CSR impedance models from steady state to transient state and from high to low energy regime; 3) Numerical implementation of the developed theory as a fast and numerical-noise-free Vlasov solver and benchmarking with massive particle tracking simulation; 4) Exploration of multistage cascaded amplification mechanism of CSR microbunching development; 5) Control of CSR-induced MBI in multi-bend transport or recirculation arcs; 6) Study of more aspects of microbunched structures in beam phase spaces; and 7) Study of MBI for magnetized beams and confirming the suppression of MBI for a recent cooler design for Jefferson Lab Electron Ion Collider project. / Ph. D. / Particle accelerators are machines to accelerate and store charged particle beams, such as electrons or protons, to the energy levels for various scientific applications. There are three basic types of particle accelerators: linear accelerators (linac), storage-ring (or circular) accelerators, and recirculating accelerators. The third type, also the most recent one, is designed to accelerate a particle beam in a short section of linac, circulate and then continue to accelerate it for energy boost or decelerate it for energy recovery. The modern recirculating machines possess the advantages to both accelerate and preserve the beam with high beam quality, as well as eciently reuse the accelerating components. As modern accelerators push toward the high-brightness or high-intensity frontier by demanding particles in a highly charged bunch to concentrate in an ever-decreasing beam phase space, the interaction amongst particles via their self-generated electromagnetic fields can potentially lead to coherent instabilities of the beam and thus pose significant challenges to the machine design and operation. Microbunching instability (MBI) has been one of the most challenging issues for such high-brightness or high-intensity beam transport, as it would degrade lasing performance in the fourth-generation light sources, reduce cooling eciency in electron cooling facilities, and eventually compromise the luminosity of colliding beams in lepton or lepton-hadron colliders. The dissertation work will focus on the MBI in modern recirculating electron accelerators. The research attempts to develop a comprehensive theoretical formulation of MBI with aspects including among various degrees of freedoms the beam itself, the beamline lattice optics, and incorporation of all relevant collective e↵ects that the beam encounters, for example the coherent synchrotron radiation (CSR) and the longitudinal space charge (LSC) e↵ects. This dissertation includes the following seven themes: 1) Development and generalization of MBI theory to arbitrary linear lattices and coupled beams with constant and varying energies; 2) Construction of CSR impedance models from steady state to transient state and from high to low energy regime; 3) Numerical implementation of the developed theory as a fast and numerical-noise-free Vlasov solver and benchmarking with massive particle tracking simulation; 4) Exploration of multistage cascaded amplification mechanism of CSR microbunching development; 5) Control of CSR-induced MBI in multi-bend transport or recirculation arcs; 6) Study of more aspects of microbunched structures in beam phase spaces; and 7) Study of MBI for magnetized beams and confirming the suppression of MBI for a recent cooler design for Je↵erson Lab Electron Ion Collider project.

Orientador: Tsunao Matsumoto / Banca: Alexandre Ninhaus Silveira / Banca: Katt Reginal Lapa / Resumo: Sistemas de Recirculação para Aquicultura (SRA) possibilitam um meio alternativo de produção de peixes para áreas com limitada disponibilidade de água. Estes sistemas de cultivo intensivo de peixes oferecem potencial de produção em pequena escala assim como em grande escala, devido a recuperação de efluentes e a reutilização da água, produzindo volumes relativamente pequenos de resíduos líquidos e sólidos. O objetivo deste estudo foi determinar a eficácia do tratamento de águas residuárias em relação à remoção de Nitrogênio Amoniacal Total (NAT), Sólidos Totais (ST), Sólidos Suspensos Totais (SST), Demanda Química de Oxigênio (DQO) e Demanda Bioquímica de Oxigênio (DBO5), Nitrogênio total (NT) e Fósforo Total (FT). Para tanto, foram introduzidos Tilápia do Nilo (Oreochromis niloticus), com 0,323kg de peso médio inicial e 17 kg m-3 de densidade de armazenamento inicial no SRA experimental. O SRA era composto de 03 tanques de plástico com capacidade de 0,20 m3, Decantador de Coluna (DC), o reator de leito fluidizado aeróbio com circulação em tubos concêntricos (BAS-CT), tendo como meio de suporte a areia de diâmetro efetivo (D10) = 0,29 mm e na sequência reator de transferência de oxigênio e remoção de CO2. A eficiência na remoção do NAT no reator BAS-CT no período de aclimatação e de estabilização foi 31,0% e 35,2%, respectivamente. A remoção de ST e SST foi de 44,44% e 71,71%, respectivamente. A eficiência alcançada de remoção no SRA da DQO foi de 64,90% e de 48,01% para DBO5. Os valores médios de remoção do NT e FT no SRA foram de 25,02% e 41,05%, respectivamente. Estes resultados indicaram que o tratamento de águas residuárias de aquicultura proposto neste estudo através do BAS-CT associado ao DC tem evitado elevadas concentrações e acumulação de poluentes dentro do SRA / Abstract: Recirculating aquaculture systems (RAS) offer an alternative means of fish production for areas that to have limited water and land availability. RAS are a highly intensive fish culture systems and offer potential production units for small and large scale operations. For effluent recovery and treated water reuse, RAS produce relatively small volumes of liquid and solids wastes. The objective of this study were to determine the efficiency of the wastewater treatment with recommended to removal of Total Ammonia Nitrogen (TAN), Total Solids (TS), Total Suspended Solids (TSS), Chemical Oxygen Demand (COD) and Biochemical Oxygen Demand (BOD5), Total Nitrogen (TN) and Total Phosphorus (TP). In this study was cultivating the Nile Tilapia (Oreochromis niloticus) with 0.323kg initial average weight and 17 kg m-3 initial storage density, cultivated in RAS. The RAS was composed with 03 plastic tanks with capacity of 0.20 m3 in each, column settler (CS), aerobic fluidized bed reactor with concentric tubes circulation (BAS-CT) with sand bed support of effective diameter (D10) of 0.29 mm and oxygen transfer-CO2 removal reactor. NAT removal efficiency was in acclimation and stabilized periods in BAS-CT reactor were 31.0% and 35.2% respectively. TS and TSS removal was 44.44% and 71.71% respectively. In this RAS, the COD removal efficiency reached was 64.90% and 48.01% for BOD5. The average removal in the NT and FT in the RAS were 25.02% and 41.05% respectively. These results indicate that aquaculture wastewater treatment proposed in this study by BAS-CT associated to CS prevents high concentrations and accumulating of pollutants within the SRA

The objective of this study was to investigate the need for domestic wastewater to receive secondary treatment when being applied to the soil by subsurface drip irrigation (SDI). SDI uniformly distributes wastewater into the soil, which optimizes the soil’s chemical, physical, and biological capacity to remove waste constituents. Because of these advantages, many regulatory jurisdictions are allowing SDI at sites that previously were prohibited from using conventional trench-based soil application systems because of shallow soil restrictions. However, most of these regulatory agencies also require that the wastewater receives secondary treatment (dissolved organic carbon reduction) before the SDI system. At issue is whether the enhanced soil-based renovation provided by SDI should eliminate the necessity for secondary treatment before SDI. Two SDI systems were installed and monitored at two sites in Tennessee. These locations were residential developments served by a septic tank effluent pump (STEP) collection system, a recirculating media filter (fine gravel media), and SDI dispersal. At both locations, SDI plots were established to receive primary treated (septic tank effluent) and secondary treated (recirculating media filter effluent) wastewater. In close proximity to randomly selected SDI emitters, soil samples were extracted. Soil cores were analyzed to determine saturated hydraulic conductivity (Ksat), and pore water samples were analyzed for nitrate, total nitrogen, total carbon, and total phosphorus. Results indicate that the primary-treated sites had lower Ksat values, higher nitrate and higher total nitrogen levels than the secondary-treated side and the background soil. Interestingly, the primary treated side had less total carbon and the background phosphorus concentration was twice that of the primary and secondary treated sides. Primary effluent showed a decrease in concentration for all constituents with increased depth. Secondary treatment does result in a higher quality effluent but is not needed when applying effluent with a SDIS.

Amplitude and Phase Shift Keying (APSK) format is one of the most attractive advanced modulation formats because of its good spectral efficiency. As the information bandwidth of the current optical fiber communication system is limited by the optical amplifier bandwidth, it is important to utilize the limited bandwidth effectively. This master thesis focuses on to study the transmission performance of the APSK format both theoretically and experimentally. At first, a theoretical study was conducted using a numerical simulation. As the Extinction Ratio (ER) of the Amplitude Shift Keying (ASK) signal affects the performances of both the ASK and the Phase Shift Keying (PSK) signals, the effect of the ER upon the transmission performance of the APSK format was studied. A clear trade-off between the performance of the ASK signal and the PSK signal due to the change of the ER was observed. Then, in order to improve the performance of the APSK format, a method to improve the transmission performance was proposed. This method was named as ¡§zero-nulling method¡¨, and it solved the trade-off issue caused by the ER. The effectiveness of this method was confirmed through the numerical simulation. Next, an experimental study was conducted. An experimental setup including 330km optical fiber transmission line was prepared, and it was used to confirm the results of the theoretical simulation. The performance trade-off between the ASK and the PSK signals due to the ER was confirmed experimentally. Finally, another experimental study was conducted. An experimental setup of 500km transmission line was used for this study. By adopting the recirculating loop experimental setup, the transmission length could be extended to a few thousand kilometers. The applicability of the ¡§zero-nulling method¡¨ was confirmed using this experimental setup.

The objective of this study was to investigate the need for domestic wastewater to receive secondary treatment when being applied to the soil by subsurface drip irrigation (SDI). SDI uniformly distributes wastewater into the soil, which optimizes the soil’s chemical, physical, and biological capacity to remove waste constituents. Because of these advantages, many regulatory jurisdictions are allowing SDI at sites that previously were prohibited from using conventional trench-based soil application systems because of shallow soil restrictions. However, most of these regulatory agencies also require that the wastewater receives secondary treatment (dissolved organic carbon reduction) before the SDI system. At issue is whether the enhanced soil-based renovation provided by SDI should eliminate the necessity for secondary treatment before SDI.Two SDI systems were installed and monitored at two sites in Tennessee. These locations were residential developments served by a septic tank effluent pump (STEP) collection system, a recirculating media filter (fine gravel media), and SDI dispersal. At both locations, SDI plots were established to receive primary treated (septic tank effluent) and secondary treated (recirculating media filter effluent) wastewater. In close proximity to randomly selected SDI emitters, soil samples were extracted. Soil cores were analyzed to determine saturated hydraulic conductivity (Ksat), and pore water samples were analyzed for nitrate, total nitrogen, total carbon, and total phosphorus. Results indicate that the primary-treated sites had lower Ksat values, higher nitrate and higher total nitrogen levels than the secondary-treated side and the background soil. Interestingly, the primary treated side had less total carbon and the background phosphorus concentration was twice that of the primary and secondary treated sides. Primary effluent showed a decrease in concentration for all constituents with increased depth. Secondary treatment does result in a higher quality effluent but is not needed when applying effluent with a SDIS.

Integrated aquaculture systems (IAS) are a type of recirculating aquaculture systems (RAS) where the wastewater is treated and returned to the fish tanks. The important difference between the two is that in an IAS, wastes from the aquaculture component are recovered as fertilizer to produce an agricultural product whereas in an RAS, waste organics, nutrients and solids are treated and discharged. A pilot marine IAS at Mote Aquaculture Research Park in Sarasota, FL was studied for this project. Water quality monitoring, measurements of fish health and growth rates of fish and plants were performed over a two-year period to determine the effectiveness of the system in producing fish and plant products and removing pollutants. The goal of this portion of the project was to develop, calibrate and evaluate a model of the system, to understand the nitrogen transformations within the Mote IAS and to investigate other potential configurations of the Mote IAS. The model was divided into the various compartments to simulate each stage of the system, which included fish tanks, a drum filter for solids removal, and moving bed bioreactor (MBBR) for nitrification and disinfection. A solids tank after the drum filter was used to store the drum filter effluent slurry, which was then divided between three treatment processes: a geotube, a sand filter followed by a plant bed, and a plant bed alone. Nitrogen species modeled were particulate organic nitrogen (PON), dissolved organic nitrogen (DON), ammonium and nitrate. Of the physical components of the IAS, models of the MBBR and the two plant raceways included physical, chemical and biological nitrogen transformation processes. The sand filter, solids tank and geotube models were simple mass balances, incorporating factional removals of each species based on the observed data. Other variables modeled included temperature, dissolved oxygen, volatile suspended solids and chemical oxygen demand concentrations. The model was built in a computer program, STELLATM, to simulate the Mote IAS. The model calibration involved experimental, literature and calibrated parameters. Parameters were adjusted until the model's output was a best fit to the observed data by minimizing the sum of the squared residuals. During the sensitivity analysis, two model parameters caused large variations in the model output. The denitrifier constant caused the most variation to the model's output followed by the denitrifier fraction of volatile suspended solids. Of the removal processes, denitrification was the largest nitrogen removal mechanism from the model, accounting for 59% and 55% of the nitrogen removed from the south and north plant raceways respectively. Plant and soil uptake represented only 0.2% of the overall nitrogen removal processes followed by 0.1% by sedimentation. Finally, the model was used to investigate other treatment designs if the Mote IAS was redesigned. The first option involved a geotube and one plant raceway in series to treat the solid waste while the second option did not have a geotube, but two plant raceways. The first option was the most effective at removing nitrogen while the second was as effective as the original system and would cost less.

Na aquacultura de recirculação são utilizados filtros biológicos para o tratamento do efluente antes que este retorne aos tanques. Estas unidades de tratamento têm como finalidade a transformação de nitrogênio amoniacal em nitrato, pois a amônia e o nitrito são tóxicos para os peixes. O nitrogênio amoniacal tende a se acumular na água de cultivo, pois é um importante produto de excreção dos organismos aquáticos e degradação da ração não consumida. Nestes filtros biológicos, os microorganismos responsáveis pelo tratamento da água residuária se encontram aderidos no meio de preenchimento, formando um biofilme. O presente trabalho teve como objetivo caracterizar as comunidades microbianas presentes no biofilme associado ao filtro biológico de uma unidade experimental de tratamento de efluente de aquacultura. Durante o experimento, realizado em dois sistemas paralelos representando unidades de aquacultura com e sem recirculação de água, também foram monitorados parâmetros de qualidade da água. Os tanques experimentais foram povoados com juvenis de tilápias-do-Nilo (Oreochromis niloticus), que foram submetidos a pesagens a cada quinze dias para avaliar seu ganho de biomassa. As bactérias foram identificadas através da técnica de análise microbiológica da hibridização fluorescente in situ (FISH). A estrutura do biofilme foi avaliada através de microscopia eletrônica de varredura. Os resultados indicam que a nitrificação teve um papel mais importante no controle da qualidade da água no sistema com recirculação em relação ao tanque sem recirculação. A análise microbiológica do meio de preenchimento do filtro revelou uma presença marcante (com proporções de Cy3/DAPI variando entre 0,5% e 7,6%) de células ativas de organismos nitrificantes (oxidadores de amônia e de nitrito), pertencendo a gêneros distintos como Nitrobacter, Nitrococcus e Nitrosomonas, além de outros grupos de presença expressiva, como bactérias filamentosas (com proporções de 11,2% a 17,3% da contagem de células marcadas com DAPI). As imagens de Microscopia Eletrônica de Varredura revelaram a natureza do arranjo destas bactérias no meio filtrante, caracterizando um biofilme bem desenvolvido, composto por diversos morfotipos microbianos. O conhecimento das bactérias que compõe este biofilme pode tornar possível a geração de melhorias que podem ser implementadas para aumentar a eficiência do sistema. / In recirculating aquaculture, biological filters are used for treating the effluent before it returns to tanks. These treatment units are intended for transforming ammoniacal nitrogen into nitrate, since ammonia and nitrite are toxic to fish. Ammoniacal nitrogen tends to accumulate in culture water, because it is an important excretion product from aquatic organisms and also due to degradation of non consumed feed. In these biological filters, microorganisms responsible for the treatment of waste water adhere to the filler, forming a biofilm. The present work intended to characterize the microbial community present in the biofilm associated to the biological filter at an experimental aquaculture effluent treatment unit. During the experiment, conducted in two parallel systems representing aquaculture units with and without water recirculation, water quality parameters were also monitored. Experimental tanks were populated with juvenile Nile Tilapias (Oreochromis niloticus), which were subjected to weighing every 15 days in order to assess their biomass gain. Bacteria were identified through the microbiological analysis technique of Fluorescent In Situ Hybridization (FISH). The biofilm structure was assessed using scanning electron microscopy. The results indicate that nitrification had a more important role in the control of water quality in the system with recirculation compared with the tank without recirculation. The microbiological analysis of the filter media revealed a significant presence (with Cy3/DAPI range between 0.5% e 7.6%) of active cells from nitrifying organisms (ammonia and nitrite oxidizers), which belonged to different genera such as Nitrobacter, Nitrococcus and Nitrosomonas, in addition to groups that had an expressive presence, such as filamentous bacteria (representing 11.2% to 17.3% of the total DAPI stained cells). Scanning Electron Microscopy images revealed the nature of the arrangement of these bacteria in the filtering media, characterizing a well developed biofilm made up of diverse microbial morphotypes. The knowledge about the bacteria making up the biofilm may enable improvements that can be implemented to increase system effectiveness.

The aim of this thesis is to assess the growth rate (and suitability) of different, (in terms of percentual representation) duoculture stocking of Euroasian perch and pikeperch, when they are breeding in the experimental conditions of intensive aquaculture. It is expected to test at least 3 different duoculture stocking and 2 single (control) stocking (100% representation of pikeperch and Euroasian perch). Culture will take place in special experimental tanks which allow quantification of uneaten feed to counting the daily feed intake. Following the own testing of growth rate (including variability), the evaluation of feed intake, survival, feed conversion, and condition indices will be performed. The main tested hypothesis is to find differences in growth and other parameters between Eurasian perch and pikeperch stocking. The own experimental testing will be preceded by a literature search on the topic.

This diploma thesis is focused on culture of pikeperch under controlled conditions and tested the optimal environmental conditions for their good growth and survival. The aim of this work was to test the effect of different oxygen saturation on survival, growth, feed intake, feed conversion rate and condition of pikeperch Pikeperch (mean body weight 10.3 g; n=810) habituated to artificial feed were placed in culture tank of the same volume of water and environmental conditions. Fish were fed ad-libitum. Three different oxygen saturations were tested in triplicate: normoxia with saturation of 85-95% O2, hypoxia with saturation of 55-65% O2 and hyperoxia with saturation of 145-155% O2. The experiment lasted 82 days and was divided into five periods. Biometric measurements of fish were performed at the end of each period. Cumulative survival, fish growth, amount of received feed, specific growth rate and condition factor were used for evaluation of our results. The results showed the positive effect of hyperoxia on feed intake and growth of fish. Slower growth was observed in hypoxia. The mean weight gain for the whole experiment was followed: normoxia 28.2 g, hyperoxia 37.7 g and hypoxia 22.9 g.

Na aquacultura de recirculação são utilizados filtros biológicos para o tratamento do efluente antes que este retorne aos tanques. Estas unidades de tratamento têm como finalidade a transformação de nitrogênio amoniacal em nitrato, pois a amônia e o nitrito são tóxicos para os peixes. O nitrogênio amoniacal tende a se acumular na água de cultivo, pois é um importante produto de excreção dos organismos aquáticos e degradação da ração não consumida. Nestes filtros biológicos, os microorganismos responsáveis pelo tratamento da água residuária se encontram aderidos no meio de preenchimento, formando um biofilme. O presente trabalho teve como objetivo caracterizar as comunidades microbianas presentes no biofilme associado ao filtro biológico de uma unidade experimental de tratamento de efluente de aquacultura. Durante o experimento, realizado em dois sistemas paralelos representando unidades de aquacultura com e sem recirculação de água, também foram monitorados parâmetros de qualidade da água. Os tanques experimentais foram povoados com juvenis de tilápias-do-Nilo (Oreochromis niloticus), que foram submetidos a pesagens a cada quinze dias para avaliar seu ganho de biomassa. As bactérias foram identificadas através da técnica de análise microbiológica da hibridização fluorescente in situ (FISH). A estrutura do biofilme foi avaliada através de microscopia eletrônica de varredura. Os resultados indicam que a nitrificação teve um papel mais importante no controle da qualidade da água no sistema com recirculação em relação ao tanque sem recirculação. A análise microbiológica do meio de preenchimento do filtro revelou uma presença marcante (com proporções de Cy3/DAPI variando entre 0,5% e 7,6%) de células ativas de organismos nitrificantes (oxidadores de amônia e de nitrito), pertencendo a gêneros distintos como Nitrobacter, Nitrococcus e Nitrosomonas, além de outros grupos de presença expressiva, como bactérias filamentosas (com proporções de 11,2% a 17,3% da contagem de células marcadas com DAPI). As imagens de Microscopia Eletrônica de Varredura revelaram a natureza do arranjo destas bactérias no meio filtrante, caracterizando um biofilme bem desenvolvido, composto por diversos morfotipos microbianos. O conhecimento das bactérias que compõe este biofilme pode tornar possível a geração de melhorias que podem ser implementadas para aumentar a eficiência do sistema. / In recirculating aquaculture, biological filters are used for treating the effluent before it returns to tanks. These treatment units are intended for transforming ammoniacal nitrogen into nitrate, since ammonia and nitrite are toxic to fish. Ammoniacal nitrogen tends to accumulate in culture water, because it is an important excretion product from aquatic organisms and also due to degradation of non consumed feed. In these biological filters, microorganisms responsible for the treatment of waste water adhere to the filler, forming a biofilm. The present work intended to characterize the microbial community present in the biofilm associated to the biological filter at an experimental aquaculture effluent treatment unit. During the experiment, conducted in two parallel systems representing aquaculture units with and without water recirculation, water quality parameters were also monitored. Experimental tanks were populated with juvenile Nile Tilapias (Oreochromis niloticus), which were subjected to weighing every 15 days in order to assess their biomass gain. Bacteria were identified through the microbiological analysis technique of Fluorescent In Situ Hybridization (FISH). The biofilm structure was assessed using scanning electron microscopy. The results indicate that nitrification had a more important role in the control of water quality in the system with recirculation compared with the tank without recirculation. The microbiological analysis of the filter media revealed a significant presence (with Cy3/DAPI range between 0.5% e 7.6%) of active cells from nitrifying organisms (ammonia and nitrite oxidizers), which belonged to different genera such as Nitrobacter, Nitrococcus and Nitrosomonas, in addition to groups that had an expressive presence, such as filamentous bacteria (representing 11.2% to 17.3% of the total DAPI stained cells). Scanning Electron Microscopy images revealed the nature of the arrangement of these bacteria in the filtering media, characterizing a well developed biofilm made up of diverse microbial morphotypes. The knowledge about the bacteria making up the biofilm may enable improvements that can be implemented to increase system effectiveness.