Human Embryonic Stem (hES) cells will potentially have important applications in regenerative medicine, drug discovery and therapeutics. However, the current methods of hES cell culture limit their use in future applications due mostly to the inability to culture undifferentiated hES cells in sufficient numbers and the concern of animal derived products used in culture methods potentially implementing an immune rejection in transplantiation recipients. This project investigated two approaches for improving the culture of undifferentiated hES cells for future application in bioreactor systems. Firstly, proteins from the conditioned medium of three hES cell supportive fibroblast lines (human fetal, human neonatal and mouse embryonic fibroblasts) were identified using two-dimensional liquid chromatography, tandem mass spectrometry. This proteomic analysis identified 175 unique proteins including those from key pathways already implicated in the maintenance of human embryonic stem cell pluripotency including the Wnt, BMP/TGF-β1, activin/inhibin and insulin like growth factor-1 pathways. Identification of proteins in fibroblast conditioned medium will aid the development of a defined medium for undifferentiated hES cell culture. The second approach examined culture of hES cells in hypoxic conditions (5% O2) compared to standard normoxic conditions in CO2 incubators (20% O2). Feeder layer and feeder free culture conditions were examined in the two O2 concentrations. hES cell colonies grown in hypoxic culture had improved percentages of pluripotent cells (judged by increases in cells expressing SSEA-4, Tra-1-60 and nanog and reduction of BMP-4), stable karyotypes and increased proliferation. The maintenance of pluripotency, apoptosis and proliferation was complicated by the detection of the cell surface marker CD30. Proteomic identification and investigation into oxidative stress indicated the presence of antioxidant enzymes such as superoxide dismutases, catalases and peroxiredoxins in human fetal fibroblast conditioned medium and their regulation of redox homeostasis may play a significant role in the maintenance of hES cell pluripotency, apoptosis and proliferation. Together these investigations significantly contribute to the current body of knowledge for the undifferentiated culture of hES cells. Identification of conditioned medium proteins and the benefits of hypoxic culture of hES cells will aid the development of a defined, reproducible method of hES cell scale-up in bioreactor systems.

This thesis proposes an idea to selectively detect the code bits in an iterative soft interference cancellation multiuser receiver. It is of a great interest to reduce the complexity of the multiuser detectors in order to achieve faster multiuser communication systems. Although the suboptimum detector has much less complexity than the optimum, the detections are made on each code bit of all users through-out every iteration. Selective detection greatly reduces the amount of calculation by re-detecting only the unreliably detected code bits from the second iteration. Simulation results show that the number of detections is significantly reduced, while the performance is maintained. Necessary background information to understand the working principles of the iterative soft cancellation receiver is presented as well. Selective detection may also be used in any other receiver structures with iterative procedures to provide much less complexity. Hence, it is able to handle much more complicated receiver structures, or implement the system to a mobile device where the computational ability is much less than at the base station.

This study is situated within the contested fields of architectural education and sustainable development. It seeks to identify ideological positions within discourses related to these fields in order to explain documented resistance to the integration of sustainable design curricula in architectural education. To understand resistance to such integration we must go beyond identifying the problem. To affect curriculum change it is necessary not only to have a design for a desired state, but also to gain the power to implement it. This assumption demands both an understanding of the power relations that support the status quo and an acceptance of curriculum development as a process of ideological struggle. Hence, efforts to reform architectural education need to be informed by an understanding of the hegemonic struggles which shape architectural curricula. Existing research in the field of sustainable design education has not focussed on such issues. International studies have not considered curricula as manifestations of a history of ideological struggle. Nor have detailed studies of sustainable design education in schools of architecture been conducted in Australia. This study has addressed these knowledge gaps by investigating histories of ideas in architectural and sustainability education. A critical discourse analysis was conducted of the handbook descriptions of architectural courses in Sydney over the last thirty years, and of courses offered in 2007 by all Australian schools of architecture. This analysis was supported by curriculum mapping to reveal the power relations inherent in architectural curricula. The research has identified strategies of hegemonic struggle which affect the hegemony of ideologies in Australian architectural education and the positioning of sustainable design curricula within this contested field. I have found that sustainable design curricula are marginalised in Australian architecture courses and that this marginalisation has been historically constructed. I have also exposed hegemonic strategies that reproduce such marginalisation within curricula.

This thesis: Origins and Destinations: Representation in the theatre of Romeo Castellucci, investigates the working methodology of the Italian theatre director, Romeo Castellucci and his company, Societas Raffaello Sanzio. It provides an account of Societas Raffaello Sanzio???s history, working methods, a detailed reading of the thematic and philosophical landscape in their works especially Genesi: from the museum of sleep, and the cycle: Tragedia Endogonidia, and a discussion on the company???s artistic process towards the formation of its compositions and performances. This research and investigation is based on numerous viewings of most of the company???s theatre works created in the last six years, interviews with Romeo Castellucci as well as other participating artists, two privileged periods of observation (residencies) in Italy of the rehearsal and creation processes of three shows, and the analysis and discussion of some of the key critical and intellectual responses to the work of Romeo Castellucci. The thematic focus of the thesis is the notion of Origins and Destinations, and its relationship with the language of representation in Romeo Castellucci???s theatre. The theoretical discussion in the thesis is organised around Giorgio Agamben???s notion of Potentiality within the composition and content of Castellucci???s theatre. This concept provides a link between the key ideas of Origins, Destinations and Representation. Castellucci???s application of Agamben???s Potentiality deconstructs dramatic structure, narrative and action down to the fundamentals of the act itself, separated from its meaningful context. It is the conclusion of this thesis, that in the instant of this singular act, Romeo Castellucci manages to represent a point where origin and destination meet, or a point where they both are, for an instant, one and the same thing.

This is a study against essentialist generalisations. Empirically, the study has been conducted to understand the food related practices among the Bengali-Muslim migrants in Sydney based on the dichotomy of Halal (permitted in Islam) and Haram (prohibited in Islam). Instead of evaluating Islam and Muslim communities as monolithic and undifferentiated this study reveals the localised actualisation of Islam which serves as a conditioning factor for these Bengali-Muslim migrants. Adopting a naturalistic methodological approach a number of ethnographic tools have been used to reveal the complex multifaceted processes through which Sydney???s Bengali-Muslim migrants negotiate the situational convergence and divergence between their ethnic identity as Bengali and their religious identity as Muslim. As a significant site of this interplay this study discovers from their food related practices that the Bengali-Muslim migrants in Sydney construct the notion of Halal-Haram food rules and regulations through the dialectics of their Bengali-informed Islam. The Bengali version of Islam poses considerable challenge to the modernist opposition between secularism and religion which is quite inadequate to understand the way the Bengali-Muslims historically negotiate both of these in the form of overlapping consensus. The findings of the study exhibit that this situationally shifting emphasis on their secular Bengali identity at one point of time and on their religious Muslim identity at another determines their decisive practices regarding food consumption in a Western cultural milieu. The Bengali-Muslim migrant participants of this study tend to perceive the notion of Halal-Haram in multiple ways so as to fit the pragmatic realities of their migrant life, which eventually leads them to reconstruct, renegotiate or even discard the scriptural/theological/authoritative discourse. Such underlying properties of food practices vindicate the argument that any stereotypically standardised notion of ???Islam??? is inadequate to understand varied Muslim migrant communities across the globe. Rather specific Muslim migrant community should be studied along with a profound understanding of their very contextual nature and historical formations.

Using written and studio research, the above research question is interrogated through a body of practical work, that evolved into a static exhibition titled Second skin: new knitting at The Ivan Dougherty Gallery, Paddington, New South Wales, Australia, in January/February 2008. This thesis examines knitting as a form of constructed textile in an historical and contemporary context and explores the integral relationship it has with fashion and textiles, as well as questioning the significance of interplay between fashion and art. The primary aim of this research is to explore contemporary knitwear designers working in the high-fashion’ area of design and investigate how they are challenging traditionally established rules and perceptions, and potentially blur the boundaries of what is considered fashion design, into art. While conceptual fashion design has always been a debateable issue among fashion scholars as to whether it can be viewed as fashion, antifashion or possibly art, this thesis examines the influences of modernity and deconstruction in relation to knitting, to establish a conclusion regarding the contemporary position and understanding of knitting in society at present. Through gathering both primary and secondary research nationally and internationally, knitting has been examined in relation to the ways in which contemporary designers working in the realm of knitting are unravelling these traditionally based preconceptions, and analysing what they are accomplishing through the use of mixed media applications, post-knitting treatments, yarn and stitch manipulation, and challenging sculptural form to create a new visual language through artisanal production. The body of work presented explores the concepts established in the written research relative to perception and deconstruction, and provokes questions which challenge the notion of knitting as fashion, art or both. Through hand and machine knitting techniques, Second skin: new knitting examines body scarification in the form of tribal markings, which allows each pieces to transform into a second skin that convey interpretive narratives and visual messages. The predominant medium utilised is 100% Australian wool and in conjunction, incorporates mixed media materials such as silk organza and semi precious stones, and technological processes include laser cutting and etching of Tasmanian oak veneer and leather.

This thesis adopts post-colonial theory as a reading strategy to address both fiction and interdisciplinary critical writing on the subject of tourism. The introduction argues for the viability of this methodology, highlighting similarities between the ideological underpinnings of tourism and imperialism, especially in relation to the power of the gaze. The first chapter draws on analyses of early exploration and travel writing to advance the argument that as the ideology of empire was encoded in travel writing, so contemporary tourist culture, in so far as it has inherited this discourse, continues to operate within it. I suggest that in much the same way as the explorer???s gaze was a form of creating knowledge disseminated through writing, fiction constitutes a cultural production that contests the power of the gaze. The second chapter focuses on the ambivalent effects of the commodification of culture caused by the tourist industry. I argue that cultural tourism, centred on heritage and history, has constituted a platform from which to review conventional representations of Australian history in a way that demonstrates the relevance of heritage to contemporary national narratives. The third and final chapter examines the relationship between cultural performances in tourism and subject formation, contending that the repetitive nature of performance offers an ideal opportunity to interpolate transformative views of both locals and tourists into the conventional tourist discourse.

The quality of visual perception with epiretinal electric stimulation strongly depends on the configuration of electrode arrays. Such arrays at the surface of the retina should excite only cells within a local area, but in practice, they exhibit some cross-talk, possibly leading to a smearing of focal activation of the retina. In this study, a passive model of extracellular voltage distribution arising from epiretinal stimulation is solved using the method of finite differences, in order to explore the voltage and current distributions of a hexagonal electrode array configuration. Results of this study suggest that the hexagonal electrode arrangement is effective in providing targeted focal activation of retinal tissue. Cross-talk between hexagonal clusters may be minimized by stimulus paradigms which utilize six return (or guard) electrodes around each stimulus electrode.

In this thesis I examine the ways in which the modern state addresses sex. I want to ascertain by what considerations the state is informed in its relationship to sex. What is behind the state???s regulation of sexual practices? What is its interest in regard to sex? To answer these questions I examine fundamental artefacts of the modern state, especially the law (but also the bureaucracy), as directed by the 1993 English court case of Brown. Brown involves the search for the sexual subject; The Lords in Brown were at a loss for how to conceptualise the subject before them. Their search is my own: who is the sexual subject? What is his relationship to the state? To answer these questions, Brown directs me for authority to two widely separated moments of supposed classic ???discontinuity???: the 1957 Wolfenden Report, and the late-Victorian Queen???s Bench. These two moments in government - the 1960s and the 1880s - are usually depicted as ideologically different, indicating discontinuity, difference, change and perhaps even revolution between the relative approaches of the state to sex. And yet, in Brown, both are upheld as appropriate contemporary authorities on sex, the individual and the state. Here I take my cue from the Lords and interrogate the artefacts of these two periods in government to ascertain the story of the 20th century state???s relationship to sex. My thesis is a political analysis that incorporates genealogy in its focus on law as indicative of the state. It incorporates a detailed study of primary artefacts of the state: detailed analyses of seemingly discontinuous moments including individual court cases, individual Committees, individual treatises and opinions and political memoirs. I conclude by drawing together my overall argument, that during the 20th century there has been no radical change of the modern state in regard to sex, and that the success of the permissive mythology has generally blinded us to this fact. Not only have we mistaken the nature of the permissive state as concerned with evolution, we have erroneously been persuaded of the blanket repression of the Victorian state. The big break, the discontinuity of the 1960s, that often is described as ???revolutionary??? (and inevitable in the teleology of progress), is a re-configuration of the same object as the Victorian state. The permissive state enacts the latest stage in the great Victorian project of embodying the sexual subject ??? a subject at once embodied and created as an object of control.

The use of stem cells to augment the healing of bone defects represents an exciting new frontier in many surgical disciplines. This thesis verified the in vitro osteogenic capability of ovine Adipose Derived Stromal Stem Cells (ADSCs) and Bone Marrow Derived Stem Cells (BMSCs), examined the in vivo osteogenic potential of the ovine ADSCs population and compared it to the in vivo osteogenic potential of ovine BMSCs. The pilot study used both cell populations, expanded and osteogenically stimulated ex vivo and mixed with resorbable porous hydroxyapatite-calcium carbonate bone graft substitute (ProOsteon 200R, Interpore Cross International, Irvine, CA). This study demonstrated the preferred length of time in ex vivo osteogenic stimulation of the ADSCs and BMSCs populations to promote the bone formation in vivo to be 7 days. In the main ovine study which employed 48 wethers, ADSCs and BMSCs were expanded, stimulated osteogenically for 7 days, mixed with ProOsteon 200R and deposited in an autologous manner into a bilateral medial femoral condyle confined cancellous defect. In vivo performance of 7 treatment groups was examined: 1. ???Bone autograft/ADSCs???, 2. ???Bone autograft/BMSCs???, 3. ???ProOsteon 200R/ADSCs???, 4. ???ProOsteon 200R/BMSCs???, 5. ???Bone autograft???, 6. ???ProOsteon 200R??? and 7. ???Empty defect???. The time-points were: 2, 4, 8 and 12 weeks. The analysis of the harvested specimens used the following methods: computerized tomography, histological assessment, histomorphometry and immunohistochemistry. There was a progressive and time dependant increase in woven bone in the defects treated with ADSCs and BMSCs across all time points. The amount of woven bone in the defects treated with the combination of ADSCs and ProOsteon 200R was comparable with the defects treated with the combination of BMSCs and ProOsteon 200R. In addition, the combination of ADSCs or BMSCs and ProOsteon 200R demonstrated no more bone than ProOsteon 200R alone. However, the ???Autograft/BMSCs??? and ???Autograft/ADSCs??? groups demonstrated a remarkable increase in the amount of woven bone formed in the defects across all time points when compared with all other groups. In addition, the amounts of bone formed in the ???Autograft/BMSCs??? and the ???Autograft/ADSCs??? group were comparable across all timepoints. The results of these studies support the hypothesis that ovine ADSCs and BMSCs populations in combination with the bone autograft can increase the formation of woven bone in the autologous orthotopic environment in a comparable manner.