Thesis (M.S.)--University of Wisconsin--Madison. / Typescript. eContent provider-neutral record in process. Description based on print version record. Includes bibliographical references (leaves 148-155).

A study was carried out to determine the relative estrogen-like activity attributed to the isoflavone compounds present in red clover plants (Trifolium pratense). The pure compounds were fed as additives to a non-estrogenic basal diet at total dosages ranging from zero to fifteen milligrams per mouse. The biological assay used for the determination of the estrogen-like activity was a slight modification of that outlined by Kitts et al. in 1959 (63). The isoflavone found to possess the greatest estrogen-like activity was Genisteim followed by Daidzein, Biochanin A and Formononetin. A non-isoflavone compound, Coumestrol, was found to possess a greater estrogen-like activity than any of the isoflavones considered. Mixtures of the pure isoflavones were bio assayed to observe any inhibitory or synergistic characteristics of one upon another. Biochanin A appeared to exert a slight inhibitory action and formononetin a synergistic character. An electrophoretic technique was developed which facilitated the detection of the isoflavones in plant extracts of monthly harvested red clover. An attempt was made to associate the biological activity of the red clover samples and the compounds which were observed in these same samples using electrophoresis / Land and Food Systems, Faculty of / Graduate

No description available.

Adventitious root growth from the crown of red clover constitutes a major portion of the root system in older stands. Two 2 yr old production fields and 3 yr old research plots were sampled in springs of 1988 and 1989 to determine the relationship between spring vigor and two root types of red clover. A higher average spring vigor rating was generally associated with the presence of well developed adventitious roots. / Five hundred and fifty-six red clover plants were dug in the fall of 1988 from a space planted (1 x 1 m centers) nursery established in the spring of the same year. Two divergent populations of 55 clones each were produced based on either a low or high score for adventitious root growth. The progeny of 32 single-crosses made within and between the two populations was evaluated for flowering habit and root types under spaced planting conditions during the 1989 growing season. / Six red clover cultivars were established in solid seedings to monitor adventitious root growth at three sampling dates.

Thesis (Ph. D.)--University of Wisconsin--Madison, 1957. / Typescript. Vita. eContent provider-neutral record in process. Description based on print version record. Includes bibliographical references (leaves 121-124).

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Call number: LD2668 .T4 1951 T83 / Master of Science

The objective of this study was to evaluate the short-term (one-year) biomass production in red clover and to obtain an estimate of the heritability of this characteristic. Progenies from parents with high biomass had higher biomass than those from parents with low biomass or having one parent in each category. The narrow-sense heritability estimated from mid-parent offspring regression was 0.23, from parent-offspring correlation was 0.22, and realized heritability was 0.15, indicating that progeny testing would be required for successful selection of populations with greater biomass production. Plants selected for high biomass production tended to have higher shoot:root ratios and flower more profusely than the parental checks and the low biomass plants. This indicates that by selecting for high biomass, red clover will be selected for more annual growth habit at the same time.

Red clover (Trifolium pratense L.) contains isoflavones, which are of interest because of their benefits for human health as well as their adverse effects on the fertility of farm animals. Isoflavone concentration in 10 cultivars ranged from 8923 to 12753 mug g-1 DM averaged across sites, harvests, and years. One cultivar, 'Start', distinguished itself with particularly low isoflavone levels. Concentrations varied according to plant part with leaves having the highest levels followed by stems and inflorescences, when averaged across maturity levels and cultivars (11970, 4896 and 3297 mug g-1 DM, respectively). Greatest content was found in leaves and stems during vegetative stages. Fresh herbage contained higher isoflavone content than either silage or hay (14464, 12200 and 11604 mug g-1 DM, respectively). Foliar application of yeast extract, chitosan, and acetic acid elicitors overall showed modest (12-15%) increases in isoflavone concentration over untreated control plants. Differences were not observed between elicitor concentrations used.