MicroRNAs represent a special class of small (~21–25 nucleotides) non-coding RNA molecules which exert powerful post-transcriptional control over gene expression in eukaryotes. Indeed microRNAs likely represent the most abundant class of regulators in animal gene regulatory networks. This study describes the recovery and network analyses of a suite of homologous microRNA targets recovered through two different prediction methods for whole gene regions across twelve Drosophila species. Phylogenetic criteria under an accepted tree topology were used as a reference frame to 1) make inference into microRNA-target predictions, 2) study mathematical properties of microRNA-gene regulatory networks, 3) and conduct novel phylogenetic analyses using character data derived from weighted edges of the microRNA-target networks. This study investigates the evidences of natural selection and phylogenetic signatures inherent within the microRNA regulatory networks and quantifies time and mutation necessary to rewire a microRNA regulatory network. Selective factors that appear to operate upon seed aptamers include cooperativity (redundancy) of interactions and transcript length. Topological analyses of microRNA regulatory networks recovered significant enrichment for a motif possessing a redundant link in all twelve species sampled. This would suggest that optimization of the whole interactome topology itself has been historically subject to natural selection where resilience to attack have offered selective advantage. It seems that only a modest number of microRNA–mRNA interactions exhibit conservation over Drosophila cladogenesis. The decrease in conserved microRNA-target interactions with increasing phylogenetic distance exhibited a cure typical of a saturation phenomena. Scale free properties of a network intersection of microRNA target predictions methods were found to transect taxonomic hierarchy.

Submitted by Renata Lopes (renatasil82@gmail.com) on 2017-09-12T20:18:35Z No. of bitstreams: 0 / Approved for entry into archive by Adriana Oliveira (adriana.oliveira@ufjf.edu.br) on 2017-09-13T13:06:30Z (GMT) No. of bitstreams: 0 / Made available in DSpace on 2017-09-13T13:06:30Z (GMT). No. of bitstreams: 0 Previous issue date: 2016-11-24 / CAPES - Coordenação de Aperfeiçoamento de Pessoal de Nível Superior / Esta pesquisa-ação apresenta uma proposta de intervenção aplicada em uma turma de 8º ano do Ensino Fundamental, de uma escola da rede pública municipal, na cidade de Juiz de Fora, no ano de 2016. Diante de um diagnóstico de baixo interesse pela leitura de livros e um repertório literário limitado, esta proposta teve por objetivo tornar a leitura literária mais atraente aos alunos, bem como ampliar seu repertório, tendo sido escolhido como ponto de partida o gênero policial juvenil, por permitir tanto o estabelecimento de uma rede intertextual horizontal, com outras obras policiais juvenis, quanto de uma rede vertical com a tradição literária do gênero. A intervenção apoiou-se teoricamente no conceito de letramento literário (Paulino e Cosson, 2009; Colomer, 2007), bem como nas propostas para a efetivação do letramento literário (Paulino e Cosson, 2009) e a formação do leitor (Colomer, 2007) na escola; no conceito de repertório literário (Iser, 1996); no conceito de polissistema literário (Even-Zohar, 2013); e no conceito de comunidade de leitores (Chartier, 1994). A execução do projeto se efetivou em três etapas, partindo da inserção dos alunos na comunidade de leitores da narrativa policial, através da leitura compartilhada de uma ficção policial juvenil, e ampliando-se seu repertório com as estratégias narrativas da tradição literária policial, usadas nos contos de Arthur Conan Doyle e Agatha Christie. Este trabalho vincula-se ao programa de Mestrado Profissional em Letras – PROFLETRAS, integrando-se à linha de pesquisa: Leitura e Produção Textual: diversidade social e práticas docentes. Os resultados desta pesquisa apontaram como eficazes as estratégias utilizadas para fomentar o letramento literário dos alunos, tanto no sentido de um maior envolvimento com a leitura, quanto no sentido de uma efetiva ampliação de seu repertório literário. No entanto, evidenciaram também que se trata de um processo contínuo, exigindo constante reavaliação e o planejamento de novas metas. / This action research presents a proposal for intervention applied to a class of 8th grade of elementary school, from a municipal public school in the city of Juiz de Fora, in the year 2016. Facing the diagnosis of low interest in reading books and a limited literary repertoire, this proposal has aimed to make the literary reading more attractive to students, as well as broaden their repertoire, having been chosen as a starting point the juvenile detective genre by allowing both the establishment of a horizontal intertextual network, with other juvenile police work, as a vertical network with the literary tradition of the genre. The intervention backed up theoretically on the concept of literary literacy (Paulino and Cosson, 2009; Colomer, 2007), as well as on the proposals for the realization of literary literacy (Paulino and Cosson, 2009) and the readers’ formation (Colomer, 2007) in school; on the concept of literary repertoire (Iser, 1996); on the concept of literary polysystem (Even-Zohar, 2013); and on the concept of readers community (Chartier, 1994). The execution of the project has been accomplished in three stages, starting with the integration of students in the readers’ community on the police narrative, through the shared reading of a juvenile detective fiction, and widening their repertoire with the narrative strategies of police literary tradition, used in tales by Arthur Conan Doyle and Agatha Christie. This work is linked to the Professional Master's program in Letters - PROFLETRAS, joining the line of research: Reading and Text Production: social diversity and teaching practices. The results of this research have showed how effective the strategies used are to promote the literary literacy of students, both in the sense of a greater involvement with reading, and in the sense of an effective extension of their literary repertoire. However, they have also showed that it is a continuous process, requiring constant re-evaluation and planning new goals.