During the first few hours of recovery from prolonged exercise, co-ingestion of protein (PRO) with carbohydrate (CHO) will increase the rate of muscle glycogen resynthesis if the rate of CHO intake is sub-optimal and/or when the feeding intervals are > 1 h apart (Burke et al.. 2004). It remains controversial whether the higher rate of glycogen resynthesis is attributable to a PRO-mediated increase in insulin release or simply the result of higher energy intake (Jentjens et al., 2001). The optimal rate of CHO ingestion necessary to maximize glycogen resynthesis remains unknown, although some studies have recommended a peak CHO intake of >1.2 g/kg/h. PURPOSE: We examined whether the addition of PRO or “extra CHO” to a drink that provided 1.2 g CHO/kg/h would increase muscle glycogen resynthesis during recovery from prolonged exercise. METHODS: Six men (22± 1 yr; V02|,eilk= 48±8 ml/kg/min) were studied during a 4-h recovery period on 3 separate occasions after a standardized 2-h exercise protocol designed to substantially lower muscle glycogen. Subjects randomly consumed 1 of 3 drinks during recovery from each trial: 1.2 gCHO/kg/h (CHO), 1.6 gCHO/kg/h (CHO/CHO) or 1.2 gCHO + 0.4 gPRO/kg/h (CHO/PRO). Drinks were consumed immediately post-exercise and at 15 min intervals for 3 hrs. RESULTS: Biopsies obtained at 0 and 4 hrs post-exercise revealed no difference in muscle glycogen resynthesis rates between trials (CHO: 22.7±6.6; CHO/CHO: 25.0±3.0; CHO/PRO: 24.6±4.2 mmol/kg dry wt/h) despite differences in energy intake between trials. The ingestion of additional CHO or PRO did not induce changes in blood [insulin ] and blood [glucose] compared to a 1.2 gCHO/kg/h beverage. Muscle [lactate] increased from immediately post-exercise to 4 hrs into recovery in all 3 trials (main effect for time. P<0.05). All subjects reported some degree of gastro-intestinal(Gl) distress after 3 and 4 hrs of recovery but there were no differences between treatments (main effect for time, P<0.05 vs. 0 hrs). CONCLUSION: Ingesting 1.2 gCHO/kg/h met or exceeded the threshold necessary to optimize muscle glycogen synthesis during the first 4 hrs of recovery from prolonged, strenuous exercise in recreationally active men. The ingestion of >1.2 gCHO/kg/h at a concentration of -20% solution induced GI distress in some individuals and may hamper subsequent same-day performance. / Thesis / Master of Science (MS)

Cladonia is one of the largest and widely distributed genera of lichen-forming fungi and some form the main winter diet for northern caribou and dominate the northern peatlands. Therefore, they are an ecologically important group of lichens. The ongoing climate change of the northern regions, impose challenges for lichen growth and algal interactions in these species. As in other organisms, genetic variability and phenotypic plasticity in the resynthesis of the lichen thallus will be important for their continued survival. The goal of my thesis is to better understand the variability of the reindeer lichens in an evolutionary and ecological context. Phylogenetic reconstruction (Chapter 2) together with genetic polymorphic studies with RAPD (Chapter 3) showed a lack of monophyly for 12 of 18 species of Cladonia and significant intraspecific genetic variation within the group. Genetic variation in the obligate fungal partner must also rely on the success of the symbiont interaction, which was further studied by resynthesis experiments. In vitro resynthesis experiments of C. rangiferina (Chapter 4) examined recognition- and defense-related genes of the symbionts of C. rangiferina using quantitative Polymerase Chain Reaction (qPCR) (Chapter 5) and suggested that the communication between partners occur and the survival and reestablishment of lichens in nature is affected by the presence of the compatible algal pool. This emphasizes the importance of finding the compatible algal partner for successful lichenization and suggests that parasitism and short-term interaction with incompatible algae may be a strategy allowing the fungus to survive until it finds a compatible algal partner. These studies also make parallels between the lichen symbiosis and plant pathogenic systems and provide molecular evidence for the parasitic nature of the lichen symbiosis. The collapse of the interaction between symbionts of C. rangiferina under varying temperature and pH conditions (Chapter 6) suggests the potential effect of habitat change on the reestablishment of lichens in nature. In summary, my Ph. D. research showed the importance of genetic and symbiotic versatility in an ecologically important species, using a model species in the group Cladonia and raised new questions for other lichen symbioses relevant to their life styles and habitats.

PCNA and RPA are required for DNA mismatch repair (MMR), but their rolesin the pathway are not fully understood. Using an affinity pull-down approach, weshow that (1) increased PCNA binding to DNA heteroduplexes is associated withthe appearance and accumulation of excision products; and (2) RPAphosphorylation occurs when DNA polymerase ?? binds to the DNA substrate. Wetherefore hypothesize that PCNA plays an important role in mismatch-provokedexcision and that RPA phosphorylation plays an important role in DNA resynthesis.To determine the role of PCNA in MMR, mismatch-provoked and nick-directedexcision was assayed in a cell-free system in the presence of the PCNA inhibitor,p21CIP1/WAF. We show that whereas PCNA is essential for 3' directed excision, it isdispensable for the 5' directed reaction, suggesting a differential role for PCNA inMMR. We further find that the PCNA-dependent pathway is the only pathway for3' directed excision, but there are at least two pathways for 5' directed excision,one of which is a PCNA-independent 5' excision pathway. To determine if RPAphosphorylation facilitates DNA resynthesis, a gap-filling assay was developedusing both a cell-free system and a purified system, and we demonstrate that RPAphosphorylation stimulates DNA polymerase ??-catalyzed resynthesis in bothsystems. Kinetic studies indicate that phosphorylated RPA has a lower affinity forDNA compared with un-phosphorylated RPA. Therefore, the stimulation ofresynthesis by phosphorylated RPA is likely due to the fact that phosphorylationpromotes the release of RPA from DNA, thereby making DNA template availablefor resynthesis.

No description available.

Os avanços da indústria de semicondutores nas últimas décadas foram baseados fortemente na contínua redução de tamanho dos dispositivos CMOS fabricados. Os usos de dispositivos CMOS dependem profundamente da lógica de portas E/OU/INV. À medida que os dispositivos CMOS estão atingindo oslimites fisicos, pesquisadores aumento esforço para prolongar a vida útil da tecnologia CMOS. Também é necessário investigar dispositivos alternativos, que em muitos casos implicam no uso de operações lógicas básicas diferentes. Como as ferramentas comerciais de síntese não são capazes de manipular eficientemente estas tecnologias Esta tese de doutorado foca em produzir algoritmos eficientes para projeto de circuitos tanto em CMOS quanto em novas tecnologias, integrando estes algorithmos em fluxos de projeto. Para esta tarefa, aplicamos a técnica da composição functional, para sintetizar eficiente tanto em CMOS quanto em tecnologias emergentes. A composição funcional é uma abordagem de síntese de baixo para cima, provendo flexibilidade para implementar algoritmos com resultados ótimos ou sub-ótimos para diferentes tecnologias. A fim de investigar como a composição funcional se compara às abordagens de síntese estado-da-arte, propomos aplicar esse paradigma de síntese em seis cenários diferentes. Dois deles se concentram em circuitos baseados em CMOS e outros quatro em circuitos baseados em tecnologias emergentes. Em relação a circuitos baseados em CMOS, investigamos a composição funcional para fatoração de funções multi-saídas, aplicadas em um fluxo de resíntese. Também manipulamos funções aproximadas, a fim de sintetizar módulos de redundância tripla aproximada. No que diz respeito as tecnologias emergentes, exploramos a composição funcional através de diodos spintrônicos e outras abordagens promissoras com base em diferentes implementações de lógica: a lógica de limiar, lógica majoritária e lógica de implicação. Resultados apresentam uma melhoria considerável em relação aos métodos estadoda- arte tanto para aplicações CMOS quanto aplicações de tecnologias emergentes, demonstrando a capacidade de lidar com diferentes tecnologias e mostrando a possibilidade de melhorar tecnologias ainda não exploradas. / The advances in semiconductor industry over the last decades have been strongly based on continuous scaling down of dimensions in manufactured CMOS devices. The use of CMOS devices profoundly relies on AND/OR/Inverter logic. As the CMOS scaling is reaching its physical limits, researchers increase the effort to prolong the CMOS life. Also, it is necessary to investigate alternative devices, which in many cases implies the use of different basic logic operations. As the commercial synthesis tools are not able to handle these technologies efficiently, there is an opportunity to research alternative logic implementations better suited for these new devices. This thesis focuses on presenting efficient algorithms to design circuits in both CMOS and new technologies while integrating these algorithms into regular design flows. For this task, we apply the functional composition technique, to efficiently synthesize both CMOS and emerging technologies. The functional composition is a bottom-up synthesis approach, providing flexibility to implement algorithms with optimal or suboptimal results for different technologies. To investigate how the functional composition compares to the state-of-the-art synthesis methods, we propose to apply this synthesis paradigm into six different scenarios. Two of them focus on CMOS-based circuits, and other four are based on emerging technologies. Regarding CMOSbased circuits, we investigate functional composition to investigate multi-output factorization in a circuit resynthesis flow. Also, we manipulate approximate functions to synthesize approximate triple modular redundancy (ATMR) modules. Concerning emerging technologies, we explore functional composition over spin-diode circuits and other promising approaches based on different logic implementations: threshold logic, majority logic, and implication logic. Results present a considerable improvement over the state-of-the-art methods for both CMOS and emerging technologies applications, demonstrating the ability to handle different technologies and showing the possibility to improve technologies not explored yet.

Os avanços da indústria de semicondutores nas últimas décadas foram baseados fortemente na contínua redução de tamanho dos dispositivos CMOS fabricados. Os usos de dispositivos CMOS dependem profundamente da lógica de portas E/OU/INV. À medida que os dispositivos CMOS estão atingindo oslimites fisicos, pesquisadores aumento esforço para prolongar a vida útil da tecnologia CMOS. Também é necessário investigar dispositivos alternativos, que em muitos casos implicam no uso de operações lógicas básicas diferentes. Como as ferramentas comerciais de síntese não são capazes de manipular eficientemente estas tecnologias Esta tese de doutorado foca em produzir algoritmos eficientes para projeto de circuitos tanto em CMOS quanto em novas tecnologias, integrando estes algorithmos em fluxos de projeto. Para esta tarefa, aplicamos a técnica da composição functional, para sintetizar eficiente tanto em CMOS quanto em tecnologias emergentes. A composição funcional é uma abordagem de síntese de baixo para cima, provendo flexibilidade para implementar algoritmos com resultados ótimos ou sub-ótimos para diferentes tecnologias. A fim de investigar como a composição funcional se compara às abordagens de síntese estado-da-arte, propomos aplicar esse paradigma de síntese em seis cenários diferentes. Dois deles se concentram em circuitos baseados em CMOS e outros quatro em circuitos baseados em tecnologias emergentes. Em relação a circuitos baseados em CMOS, investigamos a composição funcional para fatoração de funções multi-saídas, aplicadas em um fluxo de resíntese. Também manipulamos funções aproximadas, a fim de sintetizar módulos de redundância tripla aproximada. No que diz respeito as tecnologias emergentes, exploramos a composição funcional através de diodos spintrônicos e outras abordagens promissoras com base em diferentes implementações de lógica: a lógica de limiar, lógica majoritária e lógica de implicação. Resultados apresentam uma melhoria considerável em relação aos métodos estadoda- arte tanto para aplicações CMOS quanto aplicações de tecnologias emergentes, demonstrando a capacidade de lidar com diferentes tecnologias e mostrando a possibilidade de melhorar tecnologias ainda não exploradas. / The advances in semiconductor industry over the last decades have been strongly based on continuous scaling down of dimensions in manufactured CMOS devices. The use of CMOS devices profoundly relies on AND/OR/Inverter logic. As the CMOS scaling is reaching its physical limits, researchers increase the effort to prolong the CMOS life. Also, it is necessary to investigate alternative devices, which in many cases implies the use of different basic logic operations. As the commercial synthesis tools are not able to handle these technologies efficiently, there is an opportunity to research alternative logic implementations better suited for these new devices. This thesis focuses on presenting efficient algorithms to design circuits in both CMOS and new technologies while integrating these algorithms into regular design flows. For this task, we apply the functional composition technique, to efficiently synthesize both CMOS and emerging technologies. The functional composition is a bottom-up synthesis approach, providing flexibility to implement algorithms with optimal or suboptimal results for different technologies. To investigate how the functional composition compares to the state-of-the-art synthesis methods, we propose to apply this synthesis paradigm into six different scenarios. Two of them focus on CMOS-based circuits, and other four are based on emerging technologies. Regarding CMOSbased circuits, we investigate functional composition to investigate multi-output factorization in a circuit resynthesis flow. Also, we manipulate approximate functions to synthesize approximate triple modular redundancy (ATMR) modules. Concerning emerging technologies, we explore functional composition over spin-diode circuits and other promising approaches based on different logic implementations: threshold logic, majority logic, and implication logic. Results present a considerable improvement over the state-of-the-art methods for both CMOS and emerging technologies applications, demonstrating the ability to handle different technologies and showing the possibility to improve technologies not explored yet.

Os avanços da indústria de semicondutores nas últimas décadas foram baseados fortemente na contínua redução de tamanho dos dispositivos CMOS fabricados. Os usos de dispositivos CMOS dependem profundamente da lógica de portas E/OU/INV. À medida que os dispositivos CMOS estão atingindo oslimites fisicos, pesquisadores aumento esforço para prolongar a vida útil da tecnologia CMOS. Também é necessário investigar dispositivos alternativos, que em muitos casos implicam no uso de operações lógicas básicas diferentes. Como as ferramentas comerciais de síntese não são capazes de manipular eficientemente estas tecnologias Esta tese de doutorado foca em produzir algoritmos eficientes para projeto de circuitos tanto em CMOS quanto em novas tecnologias, integrando estes algorithmos em fluxos de projeto. Para esta tarefa, aplicamos a técnica da composição functional, para sintetizar eficiente tanto em CMOS quanto em tecnologias emergentes. A composição funcional é uma abordagem de síntese de baixo para cima, provendo flexibilidade para implementar algoritmos com resultados ótimos ou sub-ótimos para diferentes tecnologias. A fim de investigar como a composição funcional se compara às abordagens de síntese estado-da-arte, propomos aplicar esse paradigma de síntese em seis cenários diferentes. Dois deles se concentram em circuitos baseados em CMOS e outros quatro em circuitos baseados em tecnologias emergentes. Em relação a circuitos baseados em CMOS, investigamos a composição funcional para fatoração de funções multi-saídas, aplicadas em um fluxo de resíntese. Também manipulamos funções aproximadas, a fim de sintetizar módulos de redundância tripla aproximada. No que diz respeito as tecnologias emergentes, exploramos a composição funcional através de diodos spintrônicos e outras abordagens promissoras com base em diferentes implementações de lógica: a lógica de limiar, lógica majoritária e lógica de implicação. Resultados apresentam uma melhoria considerável em relação aos métodos estadoda- arte tanto para aplicações CMOS quanto aplicações de tecnologias emergentes, demonstrando a capacidade de lidar com diferentes tecnologias e mostrando a possibilidade de melhorar tecnologias ainda não exploradas. / The advances in semiconductor industry over the last decades have been strongly based on continuous scaling down of dimensions in manufactured CMOS devices. The use of CMOS devices profoundly relies on AND/OR/Inverter logic. As the CMOS scaling is reaching its physical limits, researchers increase the effort to prolong the CMOS life. Also, it is necessary to investigate alternative devices, which in many cases implies the use of different basic logic operations. As the commercial synthesis tools are not able to handle these technologies efficiently, there is an opportunity to research alternative logic implementations better suited for these new devices. This thesis focuses on presenting efficient algorithms to design circuits in both CMOS and new technologies while integrating these algorithms into regular design flows. For this task, we apply the functional composition technique, to efficiently synthesize both CMOS and emerging technologies. The functional composition is a bottom-up synthesis approach, providing flexibility to implement algorithms with optimal or suboptimal results for different technologies. To investigate how the functional composition compares to the state-of-the-art synthesis methods, we propose to apply this synthesis paradigm into six different scenarios. Two of them focus on CMOS-based circuits, and other four are based on emerging technologies. Regarding CMOSbased circuits, we investigate functional composition to investigate multi-output factorization in a circuit resynthesis flow. Also, we manipulate approximate functions to synthesize approximate triple modular redundancy (ATMR) modules. Concerning emerging technologies, we explore functional composition over spin-diode circuits and other promising approaches based on different logic implementations: threshold logic, majority logic, and implication logic. Results present a considerable improvement over the state-of-the-art methods for both CMOS and emerging technologies applications, demonstrating the ability to handle different technologies and showing the possibility to improve technologies not explored yet.

no / The majority of studies have demonstrated a right hemisphere (RH) advantage for the perception of emotions. Other studies have found that the involvement of each hemisphere is valence specific, with the RH better at perceiving negative emotions and the LH better at perceiving positive emotions [Reuter-Lorenz, P., & Davidson, R.J. (1981) Differential contributions of the 2 cerebral hemispheres to the perception of happy and sad faces.Neuropsychologia, 19, 609¿613]. To account for valence laterality effects in emotion perception we propose an `expectancy¿ hypothesis which suggests that valence effects are obtained when the top-down expectancy to perceive an emotion outweighs the strength of bottom-up perceptual information enabling the discrimination of an emotion. A dichotic listening task was used to examine alternative explanations of valence effects in emotion perception. Emotional sentences (spoken in a happy or sad tone of voice), and morphed-happy and morphed-sad sentences (which blended a neutral version of the sentence with the pitch of the emotion sentence) were paired with neutral versions of each sentence and presented dichotically. A control condition was also used, consisting of two identical neutral sentences presented dichotically, with one channel arriving before the other by 7 ms. In support of the RH hypothesis there was a left ear advantage for the perception of sad and happy emotional sentences. However, morphed sentences showed no ear advantage, suggesting that the RH is specialised for the perception of genuine emotions and that a laterality effect may be a useful tool for the detection of fake emotion. Finally, for the control condition we obtained an interaction between the expected emotion and the effect of ear lead. Participants tended to select the ear that received the sentence first, when they expected a `sad¿ sentence, but not when they expected a `happy¿ sentence. The results are discussed in relation to the different theoretical explanations of valence laterality effects in emotion perception.

This master thesis is concerned about the resynthesis of combinational circuits with the help of evolutional principles. The first part of this text describes logic synthesis and its weak spots, evolutional synthesis and its advantages, and also some of the existing synthesis programs. The second part shows usage of graph algorithms in logic synthesis and their possible usage in an extension for the chosen synthesis program. Suggested design and practical implementation of the extension is also described in this part. In the third part extension testing is mentioned. The fourth part is the last one and concludes gained knowledge and results.

This project deals with combinational digital circuits and their optimization. First there are presented main levels of abstraction utilized in the design of combinational digital circuits. Afterwards different methods are surveyed for optimization of combinational digital circuits. The next part of this project is mainly devoted to evolutionary algorithms, their common characteristics and branches: genetic algorithms, evolutionary strategies, evolutionary programming and genetic programming. The variant of genetic programming called Cartesian Genetic Programming (CGP) and the use of CGP in various areas, particularly in the synthesis and optimization of combinational logic circuits are described in detail. The project also discusses some modifications of CGP and the scalability problem of evolutionary circuit design. Consequential part of this thesis describes the method for evolution resynthesis of combinational digital circuits. There is description of design, especially the method of splitting circuits into subcircuits, and implementation details. Finally experiments with these method and their results are described.