Made available in DSpace on 2017-07-10T19:25:09Z (GMT). No. of bitstreams: 1 Nathalie.pdf: 1872403 bytes, checksum: 066688e7a8c000417cf984dd2ee4421c (MD5) Previous issue date: 2012-07-20 / This study aimed at evaluating the agronomic and environmental aspects as results from the continuing application of swine wastewater and mineral fertilizer in corn, black oats and soybeans. The experimental area has a history of swine wastewater application with trials that have been carried out since 2006, whose cycles under management are the seventh, eighth and ninth ones. Four doses of swine wastewater associated with the presence and absence of mineral fertilizer consisted on eight treatments applied to experimental plots. The agronomic and nutritional indices were determined in the three studied crops. The compounds of leachate collected at the beginning and end of each cycle were also recorded as well as soil characteristics at the beginning and end of each crop. The experimental design was in randomized blocks, under a 4x 2 factorial scheme with three replications. The results were submitted to ANOVA and means compared by Tukey test at 5% significance level. In maize crop, after a swine wastewater application, treated in digester, it could be observed that it could be partially used as an alternative fertilizer, since P and Mn+2 were supplemented with specific fertilization. A special management should be given to the accumulation of Fe+2, Cu+2 and Zn+2 in soil. Attention should be paid also to the levels of Cu+2, Fe+2, Mn+2, NO3-+NO2-, Na+ and Zn+2 in leachate material. In black oats crop, after two applications of swine wastewater treated in digester, it was concluded that swine wastewater can be partially used as an alternative fertilizer, since N, P and S could be supplied. Some attention should be taken with Cu+2 and Zn+2 accumulation in soil and some special care should also be given to the leaching of Cu+2, Fe+2, Mn+2, NO3-+NO2-, Na+ and Zn+2. In soybean crop, after three applications of wastewater treated in digester, the remaining wastewater could be partially used as an alternative fertilizer, since N, P, K+ and S were supplied by specific fertilization. There must have extra attention to the accumulation of Fe+2, Cu+2 and Zn+2 in soil as well as to percolation of Cu+2, Fe+2, Mn+2, NO3-+NO2-, Na+ and Zn+2 According to leachate. / O presente trabalho teve como objetivo avaliar os aspectos ambientais e agronômicos decorrentes da aplicação continuada de água residuária da suinocultura e adubação mineral, nas culturas de milho, aveia e soja. A área experimental possui histórico de aplicação de água residuária da suinocultura, com experimentos conduzidos desde 2006, os quais são o sétimo, oitavo e nono ciclos conduzidos na área. Quatro doses de água residuária da suinocultura, associadas à ausência e presença de adubação mineral, consistiram em oito tratamentos aplicados às parcelas experimentais. Foram determinados índices agronômicos e nutricionais nas três culturas, avaliadas as composições dos lixiviados coletados no início e final de cada ciclo, bem como as características químicas do solo ao início e final de cada cultura. O delineamento experimental consistiu de blocos casualizados em esquema fatorial (4x2) com três repetições. Os resultados obtidos foram submetidos à análise de variância e as médias comparadas pelo teste de Tukey a 5% de significância. Na cultura do milho, após uma aplicação de água residuária da suinocultura tratada em biodigestor, verifica-se que a água residuária da suinocultura pode ser parcialmente utilizada como fertilizante alternativo, desde que o P e Mn+2 sejam complementados com adubação específica. Cuidado especial deve ser dado ao acúmulo de Fe+2, Cu+2 e Zn+2 no solo. Devem-se atentar também para os níveis de Cu+2, Fe+2, Mn+2, NO3-+NO2-, Na+ e Zn+2 no material percolado. Na cultura da aveia, após duas aplicações de água residuária de suinocultura tratada em biodigestor, conclui-se que essa pode ser parcialmente utilizada como fertilizante alternativo, desde que o N, P e S sejam complementados. Deve-se ter atenção com o acúmulo de Cu+2 e Zn+2 no solo, e com a lixiviação de Cu+2, Fe+2, Mn+2, NO3-+NO2-, Na+ e Zn+2. Na cultura da soja, após três aplicações de água residuária de suinocultura tratada em biodigestor, constata-se que a mesma pode ser parcialmente utilizada como fertilizante alternativo, desde que o N, P, K+ e S sejam complementados com adubação específica. Deve-se ter atenção com o acúmulo de Fe+2, Cu+2 e Zn+2 no solo. Deve ser dada maior atenção à percolação de Cu+2, Fe+2, Mn+2, NO3-+NO2-, Na+ e Zn+2 de acordo com o lixiviado

Made available in DSpace on 2017-05-12T14:48:33Z (GMT). No. of bitstreams: 1 Nathalie.pdf: 1872403 bytes, checksum: 066688e7a8c000417cf984dd2ee4421c (MD5) Previous issue date: 2012-07-20 / This study aimed at evaluating the agronomic and environmental aspects as results from the continuing application of swine wastewater and mineral fertilizer in corn, black oats and soybeans. The experimental area has a history of swine wastewater application with trials that have been carried out since 2006, whose cycles under management are the seventh, eighth and ninth ones. Four doses of swine wastewater associated with the presence and absence of mineral fertilizer consisted on eight treatments applied to experimental plots. The agronomic and nutritional indices were determined in the three studied crops. The compounds of leachate collected at the beginning and end of each cycle were also recorded as well as soil characteristics at the beginning and end of each crop. The experimental design was in randomized blocks, under a 4x 2 factorial scheme with three replications. The results were submitted to ANOVA and means compared by Tukey test at 5% significance level. In maize crop, after a swine wastewater application, treated in digester, it could be observed that it could be partially used as an alternative fertilizer, since P and Mn+2 were supplemented with specific fertilization. A special management should be given to the accumulation of Fe+2, Cu+2 and Zn+2 in soil. Attention should be paid also to the levels of Cu+2, Fe+2, Mn+2, NO3-+NO2-, Na+ and Zn+2 in leachate material. In black oats crop, after two applications of swine wastewater treated in digester, it was concluded that swine wastewater can be partially used as an alternative fertilizer, since N, P and S could be supplied. Some attention should be taken with Cu+2 and Zn+2 accumulation in soil and some special care should also be given to the leaching of Cu+2, Fe+2, Mn+2, NO3-+NO2-, Na+ and Zn+2. In soybean crop, after three applications of wastewater treated in digester, the remaining wastewater could be partially used as an alternative fertilizer, since N, P, K+ and S were supplied by specific fertilization. There must have extra attention to the accumulation of Fe+2, Cu+2 and Zn+2 in soil as well as to percolation of Cu+2, Fe+2, Mn+2, NO3-+NO2-, Na+ and Zn+2 According to leachate. / O presente trabalho teve como objetivo avaliar os aspectos ambientais e agronômicos decorrentes da aplicação continuada de água residuária da suinocultura e adubação mineral, nas culturas de milho, aveia e soja. A área experimental possui histórico de aplicação de água residuária da suinocultura, com experimentos conduzidos desde 2006, os quais são o sétimo, oitavo e nono ciclos conduzidos na área. Quatro doses de água residuária da suinocultura, associadas à ausência e presença de adubação mineral, consistiram em oito tratamentos aplicados às parcelas experimentais. Foram determinados índices agronômicos e nutricionais nas três culturas, avaliadas as composições dos lixiviados coletados no início e final de cada ciclo, bem como as características químicas do solo ao início e final de cada cultura. O delineamento experimental consistiu de blocos casualizados em esquema fatorial (4x2) com três repetições. Os resultados obtidos foram submetidos à análise de variância e as médias comparadas pelo teste de Tukey a 5% de significância. Na cultura do milho, após uma aplicação de água residuária da suinocultura tratada em biodigestor, verifica-se que a água residuária da suinocultura pode ser parcialmente utilizada como fertilizante alternativo, desde que o P e Mn+2 sejam complementados com adubação específica. Cuidado especial deve ser dado ao acúmulo de Fe+2, Cu+2 e Zn+2 no solo. Devem-se atentar também para os níveis de Cu+2, Fe+2, Mn+2, NO3-+NO2-, Na+ e Zn+2 no material percolado. Na cultura da aveia, após duas aplicações de água residuária de suinocultura tratada em biodigestor, conclui-se que essa pode ser parcialmente utilizada como fertilizante alternativo, desde que o N, P e S sejam complementados. Deve-se ter atenção com o acúmulo de Cu+2 e Zn+2 no solo, e com a lixiviação de Cu+2, Fe+2, Mn+2, NO3-+NO2-, Na+ e Zn+2. Na cultura da soja, após três aplicações de água residuária de suinocultura tratada em biodigestor, constata-se que a mesma pode ser parcialmente utilizada como fertilizante alternativo, desde que o N, P, K+ e S sejam complementados com adubação específica. Deve-se ter atenção com o acúmulo de Fe+2, Cu+2 e Zn+2 no solo. Deve ser dada maior atenção à percolação de Cu+2, Fe+2, Mn+2, NO3-+NO2-, Na+ e Zn+2 de acordo com o lixiviado

Submitted by Socorro Pontes (socorrop@ufersa.edu.br) on 2017-09-25T13:10:22Z No. of bitstreams: 1 FernandaLC_TESE.pdf: 3609917 bytes, checksum: eda39920d8946614e6db4dd9bb265a99 (MD5) / Approved for entry into archive by Vanessa Christiane (referencia@ufersa.edu.br) on 2017-10-27T13:05:20Z (GMT) No. of bitstreams: 1 FernandaLC_TESE.pdf: 3609917 bytes, checksum: eda39920d8946614e6db4dd9bb265a99 (MD5) / Approved for entry into archive by Vanessa Christiane (referencia@ufersa.edu.br) on 2017-10-27T13:05:33Z (GMT) No. of bitstreams: 1 FernandaLC_TESE.pdf: 3609917 bytes, checksum: eda39920d8946614e6db4dd9bb265a99 (MD5) / Made available in DSpace on 2017-10-27T13:09:19Z (GMT). No. of bitstreams: 1 FernandaLC_TESE.pdf: 3609917 bytes, checksum: eda39920d8946614e6db4dd9bb265a99 (MD5) Previous issue date: 2017-03-09 / The high incidence of waterborne diseases is attributed to the inadequacy of sanitary sewage systems in Brazilian rural areas. The use of solar radiation has low-cost, easy operation and do not use chemicals in wastewater disinfection for agricultural use. Given the above, this study aimed at developing and validating a solar reactor for the disinfection of gray water from rural areas in the semiarid region, aiming at the reuse of the effluent. The experiments were conducted in the experimental area of the Federal Rural University of the Semi-Arid region in Mossoró, state of Rio Grande do Norte. The gray water treatment plant consists of septic tank, anaerobic filter and solar reactor. In the solar reactor, 0.1 m layers of gray water were exposed to solar radiation during the period between 8:00 AM to 4:00 PM, and aliquots were taken every two hours. The experimental tests were performed from July to December 2015, in order to evaluate the efficiency of solar disinfection by means of pH, dissolved oxygen, temperature, electrical conductivity, total suspended solids, turbidity, total coliforms, E. coli and helminth eggs. At the same time, the following environmental variables were monitored: air temperature and global solar radiation. For enumeration of helminth eggs, we used the modified Bailenger technique. For determination of fecal coliforms, we used the Colilert method. Data were subjected to descriptive statistics and analysis of variance for all analyzed characteristics. Multivariate analysis such as principal component analysis and hierarchical cluster analysis were also applied. A multiple regression analysis proposed a mathematical model that represents the inactivation of pathogens through solar disinfection. The results showed that the 8-hour solar exposure time was not enough to reduce the E. coli population to the point of allowing unrestricted reuse in irrigation, requiring longer exposure times or reduction in effluent color and turbidity. Principal component analysis summarized the initial 11 variables to 7 variables, explaining 79.46% of the total variance. The analysis of hierarchical clustering indicated the formation of four distinct groups, with determinant influence of seasonality and the time of exposure to solar radiation. According to the regression analysis, the most important variables in the solar disinfection process were cumulative solar radiation and conductivity / A elevada incidência de doenças de veiculação hídrica nas áreas rurais brasileiras é atribuída a inadequação dos sistemas de esgotamento sanitário. O uso da radiação solar é uma alternativa de baixo custo, fácil operação e não utilização de produtos químicos na desinfecção de águas residuárias para fins de aproveitamento agrícola. Diante do exposto, o presente trabalho tem a finalidade desenvolver e validar um reator solar para desinfecção de águas cinza de áreas rurais no semiárido, visando o reúso agrícola do efluente. Os ensaios foram realizados na área experimental da Universidade Federal Rural do Semi-árido em Mossoró-RN. A estação de tratamento de águas cinza foi composta por tanque séptico, filtro anaeróbio e reator solar. No reator solar foram mantidas lâminas de águas cinza de 0,1 m, que ficaram expostas à radiação solar durante o período entre 8:00 às 16:00 h, e coletadas alíquotas a cada duas horas. Os ensaios experimentais foram realizados no período de julho a dezembro de 2015, no intuito de avaliar a eficiência da desinfecção solar por meio das análises de: pH, oxigênio dissolvido, temperatura, condutividade elétrica, sólidos suspensos totais, turbidez, coliformes totais, E.coli e ovos de helmintos. Paralelamente, foram monitoradas, também, as seguintes variáveis ambientais: temperatura do ar e radiação solar global. Para enumeração dos ovos de helmintos foi empregada a técnica de Bailenger modificada, enquanto para determinação de coliformes fecais utilizou-se do método do Colilert. Os dados obtidos mediante procedimentos laboratoriais analíticos foram submetidos à estatística descritiva e análise de variância para todas as características analisadas. Utilizando-se ainda das ferramentas estatísticas multivariadas, como a análise de componentes principais e análise de agrupamento hierárquico, tal como da análise de regressão múltipla, possibilitou a elaboração de um modelo matemático que representou a inativação de patógenos através da desinfecção solar. O tempo de exposição solar de 8 horas não foi suficiente para redução do nível populacional de E.coli a ponto de permitir o reúso irrestrito para irrigação, necessitando de tempos de exposição prolongados ou redução nos valores de cor e turbidez do efluente. A análise de componentes principais promoveu a redução das 11 variáveis iniciais para 7 variáveis, explicando 79,46% da variância total. Já a análise de agrupamento hierárquico formou quatro grupos distintos, sendo determinantes a sazonalidade e o tempo de exposição solar à que as amostras foram submetidas. De acordo com a análise de regressão as variáveis mais importantes no processo de desinfecção solar foram a radiação solar acumulada e a condutividade elétrica / 2017-09-25

Conselho Nacional de Desenvolvimento CientÃfico e TecnolÃgico / A produÃÃo de feijÃo caupi (Vigna unguiculata (L.) Walp.) foi avaliada, em escala real neste projeto de pesquisa, utilizando-se a disposiÃÃo de esgoto domÃstico no solo atravÃs da infiltraÃÃo subsuperficial. A pesquisa foi realizada em dois perÃodos. O primeiro, na estaÃÃo chuvosa, durante os meses de janeiro a abril e o segundo, no perÃodo de estiagem, durante os meses de agosto a novembro de 2004. A estrutura fÃsica da estaÃÃo experimental foi construÃda nas dependÃncias da horticultura do Centro de CiÃncias AgrÃrias, no Campus do Pici da Universidade Federal do CearÃ, Fortaleza-CE, sendo constituÃda por seis canteiros de alvenaria de tijolos cerÃmicos nas dimensÃes: 11m de comprimento, 1m de largura, 0,60m de profundidade e inclinaÃÃo de 1%. Os canteiros foram impermeabilizados com manta plÃstica preta de 250 μ. O material de solo utilizado para o preenchimento dos canteiros foi retirado de uma Ãrea de emprÃstimo existente nas proximidades do local do experimento. Para anÃlise comparativa, os seis canteiros foram divididos em dois grupos de trÃs. O primeiro foi irrigado com Ãgua subterrÃnea bombeada de um poÃo amazonas, existente no local do experimento e o segundo irrigado com esgoto domÃstico bombeado da rede coletora de esgotos que passa pelo Campus do Pici. A taxa de aplicaÃÃo das Ãguas de irrigaÃÃo foi de 0,70 m3/dia, satisfazendo Ãs necessidades hÃdricas das plantas. Os canteiros foram divididos, ainda, em seÃÃes de 2 metros para melhor avaliar a produÃÃo ao longo dos canteiros. A produÃÃo foi avaliada atravÃs da contagem do nÃmero de sementes e vagens e a produtividade pelo peso de sementes por Ãrea Ãtil de 10m2 existente em cada canteiro. Os canteiros irrigados com esgoto domÃstico, no perÃodo chuvoso, apresentaram dados de produÃÃo de sementes de 248,52 Kg.hÃ-1 e 1.377,52 Kg.hÃ-1, respectivamente, em relaÃÃo aos irrigados com Ãgua. A produtividade de grÃos, no perÃodo de estiagem foi de 767,08 Kg.hÃ-1 nos canteiros irrigados com esgoto domÃstico e nos irrigados com Ãgua de 757,43 Kg.hÃ-1. O esgoto domÃstico, mesmo sendo uma fonte alternativa de Ãgua e nutrientes para as plantas, cuidados referentes ao manejo agrÃcola devem ser considerados para evitar riscos ao meio ambiente e à saÃde da populaÃÃo. Concluiu-se, portanto, que a disposiÃÃo de esgoto domÃstico no solo atravÃs da infiltraÃÃo subsuperficial, colaborou para melhor produÃÃo de feijÃo caupi, o que torna recomendÃvel està prÃtica em pequenas comunidades rurais, principalmente no semi-Ãrido do nordeste brasileiro. / The production of cowpea beans (Vigna unguiculata (L.) Walp.) was evaluated in a real scale in this research project, by using the disposal of domestic sewage into the soil through the sub-superficial infiltration. The research was carried out during two periods. The first one was in the rainy season from January to April, and the second one in the dry season from August to November, 2004. The physical structure of the experimental station was built at the Horticulture Sector of the Agrarian Sciences Center, on the Pici Campus of the Federal University of CearÃ, Fortaleza â CE, and was composed of six masonry seedbeds made of ceramic bricks with the following dimensions: 11m long, 1m wide, 0,60m deep, and 1% slant. The seedbeds were waterproofed with a black plastic blanket of 250 μ. The soil material used for the filling of the seedbeds was removed from a nearby area located by the place of the experiment. For comparative analysis, the six seedbeds were divided into two groups of three each. The first one was irrigated with underground water pumped from a deep well located in the place of the experiment, and the second one was irrigated with domestic sewage pumped from the sewage collecting pipe, which goes through the Pici Campus. The water volume used in the irrigation of the seedbeds was of 0,70 mÂ/day. The seedbeds were still divided into 2m sectors in order to better to evaluate the production along the seedbeds. The production was evaluated through the counting of the number of seeds and pods, and the productivity was assessed by the weight of seeds per useful area of 10,00m located in each seedbed. In the rainy season, the seedbeds irrigated with domestic sewage presented average seed production data of 248,52 Kg ha-1, and the ones irrigated with raw water showed an average production of 1.377,52 Kg ha-1. The average productivity of grains in the dry season was of 767,08 Kg.ha-1 in the seedbeds irrigated with domestic sewage, and 757,43 Kg.ha-1 in the ones irrigated with water. The domestic sewage, despite being an alternative source of water and nutrients for the plants, cares regarding the agricultural handling should be considered to avoid risks to the environment and the health of the population. It was concluded, therefore, that the disposal of domestic sewage into the soil through the sub-superficial infiltration contributed to a larger production of cowpea beans, which makes this practice advisable in small rural communities, mainly in the semi-arid region of the Brazilian northeast.

La réutilisation des eaux usées traitées pour l’irrigation est globalement acceptée et pratiquée pour faire face à la pénurie d'eau et économiser les ressources de haute qualité. Bien que cette pratique présente des avantages indéniables et contribue à un usage plus durable de l'eau douce, elle n’est pas exempt de problèmes liés à l'impact potentiel sur la qualité des sols récepteurs et sur les cultures de micropolluants contenus dans l'eau réutilisée. Parmi ces polluants, les métaux lourds (ML) en concentrations traces jouent un rôle primordial en raison de leur présence systématique dans l'eau utilisée et de leur persistance une fois libéré dans l'environnement. Le devenir des ML dans les sols peut difficilement être prédit parce que les mécanismes de mobilité à travers les sols sont extrêmement variés et liés à des phénomènes simultanés et très complexes impliquant différents équilibres chimiques. Les ML, comme beaucoup d'autres contaminants, ne sont pas seulement partagé entre la phase immobile (le sol) et les phases mobiles présentes dans l'eau. En effet, les colloïdes et les nanoparticules agissent comme une troisième phase mobile, avec leurs propres propriétés rhéologiques et des vitesses de migrations qui leur sont propres. Ce dernier aspect a été l'un des principaux objectifs d’étude de la thèse. Plusieurs essais expérimentaux ont été menés en irriguant un sol standard selon l'Organisation de coopération et de développement économiques (OCDE) avec une eau usés traités réel et / ou synthétiques, contenant des ML en concentrations traces. Pour chaque test, un sol spécifique (avec différentes teneurs en matière organique) et des eaux usées traitées de composition différente (avec différentes concentrations en métaux traces, de salinité, de la teneur en matière organique pour les eaux usées synthétiques, ou des eaux usées traitées réelles) ont été choisi afin d'évaluer les effets des conditions différentes sur le devenir global des ML. L'augmentation de la matière organique du sol de 2,5 à 10% a linéairement amélioré la mobilité des Cd, Cu et Ni avec une augmentation de la mobilité maximum de 35,6, 43,7 et 49,19% pour le Cd, Cu et Ni, respectivement. Pour la plupart des expériences, les ML ont été capturés dans la couche superficielle du sol (0,5 à 1 cm). Néanmoins, des pics de contamination ont été détectés à des profondeurs différentes dans les couches plus profondes du sol. L’étude de la composition des lixiviats montre des variations de concentrations fonction du métal étudié et des caractéristiques du sol et des eaux usées. Des pics de métaux dans le lixiviat sont apparus en même temps que la libération de la matière et / ou la libération de silicates organiques, ce qui démontre l'implication significative des colloïdes dans le transport des métaux. La concentration en sodium (20 mM) a été démontrée un impact fort sur la réduction de la mobilisation colloïdale et que plus de 95% du métal apporté a été détecté dans la couche superficielle du sol en dépit de sa teneur en matière organique. La salinité affiche donc des effets significatifs. L'irrigation avec des eaux usées traitées présentant une très haute teneur en Ca et Mg (111 et 134 mg / L, respectivement) a abouti à la libération moyenne plus élevée de silicium à partir de la matrice inorganique du sol (8,2 mg / L) par rapport à la faible salinité des eaux usées artificielle (1,9 mg / L). Par conséquent, la mobilisation ultérieure de Cd, Cu, Ni et Zn a été observée lorsque le sol a été irrigué avec des eaux usées traitées réelles. Une caractérisation spectroscopique avancée des lixiviats a été réalisée pour identifier les agrégats colloïdaux libérés par le sol dans le but d’en déterminer leur nature, leurs propriétés chimiques et leur état d'agrégation / Reuse of treated wastewater for agricultural purposes is worldwide accepted and practiced to face water scarcity and save high quality resources. Although such practice has undoubtable advantages and is certainly more sustainable respect to the use of fresh water, it is not exempt from severe concerns related to the potential impact on the receiving soil and on the crops of potentially harmful pollutants contained in the reused water at trace levels. Among these pollutants, trace heavy metals (HMs) play a primary role due to their spread presence in the used water and to their persistence once released in the environment. The fate of HMs in the soils can be hardly predicted as mechanisms of mobility through soils are extremely diverse and related to highly complex simultaneous phenomena and chemical equilibria. HMs, in fact, as many other contaminants, are not only partitioned between the solid immobile and the water mobile phases. Indeed, colloids and nanoparticles act as a third mobile phase, with their own rheological properties and velocity. This latter aspect has been one of the main focus of the thesis. In details the thesis describes the results of several experiments conducted irrigating the OECD standard soil with real and/or synthetic wastewater, containing HMs in trace. For each test a specific soil (e.g. varying the organic matter content) and wastewater composition (e.g. varying the metals concentration, the salinity, the organic matter content, or testing real treated wastewaters) has been chosen in order to evaluate the effects of different conditions on the overall HMs fate. The increase of soil organic matter from 2,5 to 10% linearly enhanced the mobility of Cd, Cu and Ni up to a maximum mobility increase of 35.6, 43.7 and 49.19 % for Cd, Cu and Ni, respectively. In most experiments metals accumulated in the top soil layer (0.5 - 1 cm). Nevertheless peaks of contamination were detected at different depths in the soil deeper layers and at different leaching time in the leachates depending on the metal and on the soil and wastewater characteristics. Peaks of metals in the leachate appeared simultaneously with release of organic matter and/or release of silicates, demonstrating outstanding involvement of colloids in metals transport. Sodium concentration (20mM) decidedly reduced colloidal mobilization whereas more than 95 % of the influent metal was detected in the top layer despite the soil organic matter content. Salinity displayed different effects. The irrigation with real treated wastewater with quite high content of Ca and Mg (111 and 134 mg/L, respectively) resulted in higher average release of silicon from the soil inorganic matrix (8.2 mg/L) compared to the low salinity artificial wastewater (1.9 mg/L). Consequently higher mobilization of Cd, Cu, Ni and Zn was observed when the soil was irrigated with real treated wastewater. An advanced spectroscopical characterization of the leachates was performed to identify such colloidal aggregates. The observation of 3D excitation-emission matrix demonstrated in all the leachates samples the presence of fulvic (230-450 nm ex-em fluorescence area) and humic (330-445 nm ex-em) substances. In this context, a novel analytical method was developed to quantify phenolic substances in soil matrices allowing the monitoring of humic matter migration in soil profiles. The novel method was more accurate and more precise respect to the traditional one, allowing to obtain higher recovery of total phenols in peat soil (15.5 % increase) with a decrease of the coefficient of variation (30.1% decrease). Organic water soluble colloids were extracted from the peat used to prepare the OECD standard soil and characterized. Results of size exclusion chromatography highlighted the supramolecular structure of the extracted organic matter. Such structure was further confirmed through fluorescence and 1H-NMR spectroscopy