Some species of the genus Aesculus are every year heavily infested by horse chestnut leaf miner (Cameraria ohridella), whose larvae are mining the leaves, and under appropriate conditions may damage up to 100% of the leaf area. The effect of lower defense capability of infested trees lead to invasion of fungal (Erysiphe flexuosa, Guignardia aesculi) and bacterial (Pseudomonas syringae pv. aesculi) pathogens, and later to higher mortality of infected individuals. In this study were measured the genetic diversity of species A.hippocastanum, A. turbinata, A. glabra, A. parviflora, A. glabra var. arguta, A. x carnea, A. marilandica, A. pavia, and their genotypes with different susceptibility to the leaf miner. Analysis of 8 microsatellite loci, using the SB buffer for separation, showed high polymorfic information content 0.45 -- 0.77 (0,60 in average) and high genetic diversity. For each loci was found average 5.5 aleles. During two vegetation period, leaf area damage of these genotypes was evaluated in statistical software Assess 2.0. Different pressure of leaf miner and different development at each locality was recorded, and even in some cases lower damage was found, in one case it occured permanently. According to the data obtained, the pathological scale was established and the dendogram of similarity was created. Three groups of resistant, non -- resistant and immune individuals were distinguished and five main section of Aesculus was found. By cloning and analysing the inner transcribed spacers, ITS1 and ITS2, inner and inter species variability was examined. For the sequence of hybrid species A. x carnea no compliance was found in NBCI, therefore first sequence of this genotype was obtained.

This thesis deals with a survey of provenance areas and assessment of attacking Fraxinus excelsior L. and Fraxinus angustifolia by the pathogen Hymenoscyphus fraxineus. The aim of the thesis is to map the incidence of infected trees in these areas and to evaluate the sensitivity of the sites to the pathogen Hymenoscyphus fraxineus. The theoretical part deals with processing of data collected during field work on selected provenance areas Koneprusy and Veltruby. Amongst the evaluated parameters there were: trunk diameter at measuring height, tree height, and especially canopy drying caused by Hymenoscyphus fraxineus. As the outcome of the survey photographs were taken and records were collected for individual trees in the provenance areas. Each of the trees has its own unique label that has accurately defined its location to prevent potential errors in data processing. The processed data was then evaluated in NCSS 8.0 software and cluster analysis was performed in order to compare the provenances. This analysis only compares the provenances, which are located in both provenance areas. The results show that no provenance without the pathogen Hymenoscyphus fraxineus attack appeared in the provenances Koneprusy and Veltruby. Subjects in 4 ŠLP Kostelec nad Černými Lesy - Svojšice and 24 LS Czech Krumlov Chvalšiny proved to be more resistant than others. Although the increase in the tree height and the trunk thickness is the largest on those areas the defoliation rate is high. It is probable that the population of these provenances will gradually die. Due to growing abbility of some individuals located in other provenances it is obvious that habitat conditions and individual resilience are key factors.

Angl. resumé

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The aim of the diploma thesis is the analysis of the occurrence of endoparasites in horses and proposing prevention options using unconvential products and pasture management. In total 20 horses were included in the observation, who were all stabled on one farm. The observation took place during on pasture season. The first faecal examination was carried out for purpose of quantitative determination of parasites using the modified McMaster technique. The faecal analysis was carried out always before and after use of the homeopatihc and allopathic preparation. Horses were found to be infected by strongylides. Coprological analysis proved resistence to pyrantel embonate in 25 % of horses. After subsequent homeopathic treatment, the efficacy of this preparation was found in several horses, with EPG (eggs per gram) not exceeding 200. The effect of season on the rate of infection was monitored, when the effect of season wasn´t statiscially confirmed (p = 0,28). In assessing the effect of age on the intensity of infection, it was confirmed that older horses had lower parasitic infection rates than the young horses. After administration of active substance ivermectin to both groups of horses, a significant difference was observed when using this active substance against pyrantel embonate, which showed an excellent effect in 95 % horses. The homeopatic preparation of PVB etat vermieux can be used as an aditional preventive treatment during the year in horses that are sensitive to it. At the same time, it is important to regularly perform coprological faecal analysis and suitably choose an allopathic remedy, it is important to focus on preventive measures in horse breeding and pasture.

Charles University Faculty of Pharmacy in Hradec Králové Department of Pharmacology and Toxicology Student: Tereza Kněžíková Supervisor: PharmDr. Ivan Vokřál, Ph.D. Title of diploma thesis: Liver fluke - treatment and resistence Fasciola hepatica is a parasite of global importance that we find both in farm animals and in humans. This thesis aims to summarize information on the potential of drugs and treatment alternatives that are suitable for treatment of F. hepatica. Given that for a number of drugs used in the past, as well as the drugs currently administered, resistance developed, this thesis is also focused on this phenomenon, especially the mechanisms of its origin. The drugs used to treat fasciolosis are called antitrematodal drugs. They can be divided into five chemical groups, of which the most important group are currently benzimidazoles and their representative triclabendazole. Also other drugs as albendazole, clorsulon, hexachlorophene, closantel, diamphenitide, bithionol, rafoxanide are important. The rate of resistance development is affected by many factors that may be genetic, biological or functional. F. hepatica actively uses its enzymatic system, especially oxidation enzymes or efflux transporters. The influence on the development of resistance, apart from the parasite itself,...

The need for new antibiotics and other biologically active compounds is the reason for an increased interest in secondary metabolites of soil bacteria. The phylum Actinobacteria has the dominant position in the soil environment thanks to the potential of producing a broad spectrum of antibiotics and the presence of a number of defense mechanisms preventing the effects of antibiotics. The aim of this thesis was to determine the number of copies of selected secondary metabolic genes in the soils of two sites using designed primers and primers from literature. The design of effective new primers for the detection of selected genes in the soil environment was not achieved in this work, and therefore only primers from literature that had been verified for their specificity were used. In samples taken from soil profiles of two sites, abundances of bacteria, actinobacteria, type II polyketide synthase genes and Erm methyltransferase genes mediating resistance to MLSB antibiotics (macrolides, lincosamides and streptogramins B) were determined by digital PCR. The comparison of the determined copy numbers gave an information about the structure of the bacterial community and the relative abundance of bacteria carrying selected secondary metabolic genes depending on the soil condition changes due to the...

This diploma thesis studies the evaluation of wine grape PIWI varieties for the production of red wines. The experiment took place in autumn 2016, specifically in September and October. Nine type of interspecific varieties, were studied and two control varieties were studied. All varieties come from the Mikulov wine-growing subregion from the Lednice village in Moravia. For all varieties, the basic analytical parameters of the qualitative characters were determined. Everything was statistically evaluated and compared. The literary part deals with problems of resistance, propagation, occurrence and description of the three most serious diseases that have just begun to breed these resistant varieties. For this research is a brief look and description of each variety separately, their origins, ampelographic features, suitable habitat and oenological properties.