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In order to study the influence on blue-green algal nitrogenase activity of environmental variables in deepwater rice-fields (DWR), a laboratory study was planned on a DWR isolate of Calothrix (D764). The variables chosen were light, oxygen, combined nitrogen, phosphorus and iron. As availability of P is likely to play an especially important role for growth and nitrogen fixation in DWR, studies on phosphatase activity of the isolate were also included. The method used for measuring nitrogenase activity was acetylene reduction assay (ARA). In order to convert nitrogenase activity to nitrogen fixation, the conversion ratio of N(_2) : C(_2)H(_2) reduced was determined by comparing the total amount of N fixed with total C(_2)H(_2) reduced. The ratio was 1 : 4.1 and 1 : 5.2 at 85 and 10 µmol photon m(^-2) s(^-1), respectively. Changes in nitrogenase activity in batch culture were studied in relation to growth characteristics. Maximum activity (10.5 nmol C(_2)H(_4) mg d. wt(^-1) min(^-1)) was observed after two days of growth. During this period, juvenile trichomes (hence maximum heterocyst frequency) were abundant and cyanophycin granules were absent; chl a, phycobiliprotein and algal N decreased. It is suggested that the juvenile filament is the most active nitrogen-fixer during the growth of the alga. The response of nitrogenase to changes in light flux (down- or upshift) was rapid. The alga showed a marked drop in nitrogenase activity in the dark, but subsequent changes were slow, with detectable activity after 24 h. Higher nitrogenase activity was observed when the dark grown alga was re-illuminated, than the maximum activity found under continuous illumination. Nitrogen fixation and heterocyst differentiation were suppressed when 10 mg 1(^-1) NH(_4)-N was added to a batch culture. Fe-deficient cultures had lower nitrogenase activity and N content than Fe-sufficient cultures. Fe- deficiency led to the development of a series of new heterocysts apical to the basal ones. Addition of Fe to Fe-deficient cultures led to a marked increase in nitrogenase activity and loss of the degenerated basal heterocysts. The alga was capable of using a number of organic P substrates as the sole source of phosphorus and showed both cell-bound phosphomono- and phosphodiesterase activities. In batch culture, phosphatase activity was detected when cellular P content dropped to 0.98%. A brief study on the influence of the environmental factors on cell-bound phosphatase activities of the alga has been included. A brief comparison in nitrogenase activity of a UK field Rivularia population and bacterised laboratory isolate Rivularia D403 was made and probable behaviour of algae in DWR is discussed.

Les rizières constituent des habitats favorables pour les oiseaux d’eau, en particulier en tant que halte migratoire, zone de reproduction ou site d’alimentation hivernal. Dans ce dernier cas, la disponibilité des ressources alimentaires (grains de riz non ramassés, graines d’adventices ou invertébrés) semble être fortement influencée par les pratiques culturales post-récolte. Ce projet vise à tester des pratiques post-récolte alternatives au brûlage-labour traditionnellement mis en place en Camargue afin de mesurer les conséquences en termes de disponibilité de nourriture et de fréquentation des parcelles par les canards hivernants. Différents traitements post-récolte (inondation, brûlage, labour, disquage et leurs combinaisons) ont été testés. Nous avons réalisé un suivi de la quantité de graines (riz, adventices) disponibles au cours du temps, depuis la récolte en Septembre-Octobre jusqu'en Mars ainsi qu’un suivi de l'usage des parcelles par les canards pendant la journée et pendant la nuit. En parallèle, nous avons étudié les bénéfices agronomiques potentiels de certaines pratiques en interculure et nous avons cherché à identifier une solution mutuellement bénéfique, répondant aux besoins d’habitat d'hivernage des canards et à ceux des riziculteurs, le tout à moindre coût. / Rice fields provide foraging habitat to waterfowl during migration, breeding and wintering seasons. In the latter case, food availability (waste rice, weed seeds or invertebrates) seems to be strongly influenced by post-harvest agricultural practices. This project aims at testing alternative post-harvest practices to the traditional burning-mowing in the Camargue in order to assess their consequences in terms of food availability and field use by wintering ducks. Different post-harvest treatments (flooding, burning, mowing, disking and their combinations) were conducted. The amount of seeds (rice + weeds) available through time was assessed, from harvest in Sept-Oct until March, and duck use of these fields during the day and during the night was simultaneously monitored. In parallel, we studied potential agronomic benefit of specific post harvest practices and we searched a mutually beneficial solution that provides needed wintering ducks habitat and is acceptable to rice farmers with limited costs.

Made available in DSpace on 2015-03-05T16:19:35Z (GMT). No. of bitstreams: 0 Previous issue date: 27 / Nenhuma / O Parque Nacional da Lagoa do Peixe (PNLP), na zona costeira do Rio Grande do Sul, protege amostras importantes de quase todos os ecossistemas de áreas úmidas e espécies de aves aquáticas da região. Os movimentos migratórios e os deslocamentos entre áreas úmidas determinam importantes variações temporais de composição e abundância das assembléias de aves aquáticas. Os objetivos deste estudo foram descrever os padrões de composição e abundância de aves aquáticas em 27 áreas úmidas naturais, distribuídas no entorno do Parque Nacional da Lagoa do Peixe; descrever os padrões de variação temporal das assembléias associados às variações de pluviosidade; avaliar o efeito do tamanho dos fragmentos, do tipo de área úmida, do tipo de matriz circundante e da disponibilidade de áreas úmidas na paisagem na composição, riqueza e abundância de aves aquáticas e discutir a importância dos remanescentes de áreas úmidas do entorno do Parque para a conservação de aves aquáticas. Foram realizados 12 censos mensais de aves aquátic / The Lagoa do Peixe National Park (LPNP), in the coastal plain of Rio Grande do Sul, protect important samples of almost all wetland ecosystems and waterbird species in the region. The surrounding areas also harbor remnant natural wetlands, scattered in a matrix of drained meadows or rice fields, of which the conservation significance is unknown. The structure of waterbird assemblages in these remnants is expected to vary with their spatial attributes and with the characteristics of the surrounding matrix. The aim of this study was to describe the composition, richness and abundance of waterbird assemblages of 27 remnant wetlands; to describe the seasonal variation in the structure of these waterbird assemblages and it’s relation with pluviosity; to evaluate the effect of the remnant’s area, type of wetland, type of matrix and wetland availability in the landscape on the composition, richness and abundance of waterbirds; and to discuss the importance of the remaining wetlands nearby the Park for the conservat