The Texas rice industry has undergone many changes over the course of the industry’s existence. Recently, high costs of production and the structure of government payments have contributed to a decreasing trend in rice acreage planted in Texas. While Texas was once the top rice producer in the United States, it now ranks fifth. Despite the fact that Texas has one of the lowest levels of production among rice producing states, it currently maintains the highest per acre yields. One of the major factors in maintaining superior yields is the development of high performance rice varieties and hybrids, which provide increased yields on fewer acres. Research institutions invest a great deal of time, effort, and money towards the development of new varieties every year. Each one of these varieties has a specific set of traits that are believed to be in high demand by producers and processors. However, during the developmental stages, scientists are uncertain as to how each new gerplasm will perform once it reaches the market. This study develops a regression model, which includes competition and the characteristics of a specific variety, to estimate the life cycle of new varieties and hybrids. In addition, simulation techniques are utilized to incorporate risk into the life cycle, providing a more robust prediction of the cumulative adoption and disadoption path. Results indicate that the life cycle of new rice varieties is becoming shorter over time. Furthermore, the length of the life cycle is directly related to a new seed’s performance, compared to other varieties on the market. Varieties that provide higher levels of performance, especially higher yields, tend to have a longer life cycle and achieve a larger market share, on average.

京都大学 / 0048 / 新制・課程博士 / 博士(農学) / 甲第21165号 / 農博第2291号 / 新制||農||1060(附属図書館) / 学位論文||H30||N5139(農学部図書室) / 京都大学大学院農学研究科地域環境科学専攻 / (主査)教授 縄田 栄治, 教授 舟川 晋也, 教授 松浦 健二 / 学位規則第4条第1項該当 / Doctor of Agricultural Science / Kyoto University / DGAM

Rice production plays an important role in the economy of the Mekong River Delta (MRD), but rice production is endangered by sea-level rise and the associated increased incidence of salinity intrusion. This study examines the diffusion of salt-tolerant rice varieties (STRVs) in the MRD that were promoted through Consortium for Unfavorable Rice Environment (CURE) activities. Evidence is found of widespread adoption in salinity-prone areas, with CURE related varieties covering 47% of rice area in at least one of two growing seasons surveyed, but that adopting areas are highly clustered. Multivariate analysis reveals that location characteristics associated with high risk of salinity inundation, rather than individual characteristics associated with household risk preferences, explain the observed pattern of adoption in the MRD. In particular, CURE-related varieties are disproportionately likely to be adopted in non-irrigated areas and in irrigated areas that are not protected by salinity barrier gates. The results imply that CURE has effectively targeted unfavorable rice growing environments and that efforts to further diffuse STRVs need to both increase the area of suitability through further varietal adaptation and promote adoption in existing suitable areas by taking advantage of strong neighborhood externalities in household adoption decisions. In terms of varietal performance, inconclusive evidence is found of higher yields of CURE-related varieties in a low-salinity year. Further, any yield gains are more than off-set by lower market prices for CURE-related varieties. / Rice is a staple crop in the Vietnamese diet and one of Vietnam's leading exports. The Mekong River Delta (MRD) accounts for more than 90 percent of rice exports. However, rice production in the MRD is endangered by saltwater intrusion due to rising sea-levels. Farmers have adopted rice varieties that are tolerant to rice to reduce their production risk that were promoted through Consortium for Unfavorable Rice Environment (CURE) activities. This study examines the rates of adoption of these CURE-related varieties, the reasons farmers choose CURE-related varieties, and variety performance on farmers' fields. Results from a household-level survey show at 47% of fields in salinity-prone areas of the MRD grow a CURE-related variety in at least one of the areas two main rice-growing seasons. Farmers are particularly likely to adopt CURE-related varieties on fields that are not protected against salinity intrusion by gates. Adoption decisions are also highly correlated with neighbors’ decisions within villages. Finally, CURE- and non-CURE-related varieties yields are similar in a year with low levels of salinity intrusion. But revenues from CURE-related varieties are slightly lower due to their lower market price, suggesting CURE-related varieties are a relatively low-cost insurance policy for MRD rice farmers in salinity-prone areas against future salinity intrusion.

京都大学 / 0048 / 新制・課程博士 / 博士(農学) / 甲第20716号 / 農博第2245号 / 新制||農||1053(附属図書館) / 学位論文||H29||N5082(農学部図書室) / 京都大学大学院農学研究科生物資源経済学専攻 / (主査)教授 福井 清一, 教授 伊藤 順一, 教授 梅津 千恵子 / 学位規則第4条第1項該当 / Doctor of Agricultural Science / Kyoto University / DGAM

The study shows that rice water has a strong inhibitory efficacy against various AGEs and the bran layer of rice is mainly responsible for its anti-glycation activity. There are many unknown bioactive compounds in bran with anti-glycation potential. Thus, stable bioactive compounds responsible for anti-glycation of rice bran are yet to be identified. The clinical data suggests that DBR or SARFR when compared with polished rice, contributes to health promotion, including skin condition, by reducing the indigestibility of brown rice and ensuring nutrition, which facilitates continuous intake. / 博士(理学) / Doctor of Philosophy in Science / 同志社大学 / Doshisha University

Genetic resources are important for the development of every country and for humanity. Collection, conservation and reasonable utilization of genetic resource is required mission. Understanding the importance of genetic resource, especially rice germplasm, since 2001, Center for conservation and development of crop genetic resources (CCD-CGR) of Hanoi University of Agriculture (Vietnam National University of Agriculture) has been collected, conserved and evaluated rice germplasm from different provinces of Vietnam for breeding programs. So far, 1090 accessions of local rice of Vietnam have been collected. Evaluation of agronomic properties and screening of some important genes using DNA molecular markers have revealed that Vietnamese rice germplasm has high level diversity and containing important genes for quality and resistance for disease and pests. These genetic resources are potential materials for national breeding programs. Based on the collected germplasm, 3 new glutinous rice varieties have been successfully created with high yield and good quality. In addition, the degradation of local rice varieties is also a matter of concern. So far, 4 specialty rice varieties Deo Dang, Ble chau, Pu de and Khau dao have been successfully restored for the north provinces of Vietnam. The main results of this study are germplasms for rice breeding programs and new improved varieties that bring economic benefits to farmers and the country. / Nguồn gene là tài nguyên sống còn của mỗi quốc gia và của toàn nhân loại. Vì vậy thu thập, bảo tồn, đánh giá và khai thác hợp lý nguồn tài nguyên này có ý nghĩa rất lớn. Nhận thức được tầm quan trọng của nguồn gen nhất là nguồn gen cây lúa, ngay từ đầu những năm 2000, Trung tâm bảo tồn và phát triển nguồn gene cây trồng thuộc Trường Đại học nông nghiệp, nay là Học Viện nông nghiệp Việt Nam đã tiến hành thu thập, lưu giữ, đánh giá và khai thác nguồn gene lúa. Kết quả đã thu thập, lưu giữ được 1090 mẫu giống lúa địa phương Việt Nam. Đánh giá đặc điểm nông sinh học và phát hiện một số gene quy định các tính trạng chất lượng và kháng sâu bệnh bằng chỉ thị phân tử DNA. Đây là nguồn gene quan trọng cho chọn tạo giống. Dựa vào nguồn gene thu thập được, cho đến nay, Trung tâm bảo tồn và phát triển nguồn gene cây trồng đã lai và chọn tạo được thành công 03 giống lúa nếp chất lượng cao. Ngoài ra, thoái hóa giống cũng là vấn đề đang được quan tâm. Cho đến nay 4 giống lúa đặc sản Đèo đàng, Ble châu, Pu đe và Khẩu dao đã được phục tráng và đưa vào sản xuất. Kết quả của những nghiên cứu này là ngân hàng các giống lúa làm nguồn gene để chọn tạo giống mới đem lại lợi ích kinh tế cho người nông dân và đất nước.

Rice (Oryza sativa L.) is the second most important cereal crop in Malawi. Rice productivity in the dominant (85%) rainfed ecosystem is very low, with mean yield of only 1.4 t ha-1. Farmers plant traditional landraces that have low yield potential and are susceptible to various stresses such as drought and diseases. Development and introduction of improved rice varieties, with stress tolerance traits from introduced varieties, such as the New Rice for Africa (NERICAs), could significantly increase productivity. Previous attempts to introduce high yielding irrigated varieties into the dominant rainfed ecosystem in Malawi have not been successful because farmers basically did not adopt the varieties, claiming that the varieties were lacking in grain traits that they preferred but that the traits were present in their landraces. The notable traits mentioned, through previous informal surveys, were long grains, medium to slender shape and aromatic grain with intermediate gelatinization temperature. No formal study has been conducted to ascertain the preferences, and the genetic control of the traits, including yield and yield related traits, have not been studied. The objectives of this study were to: 1) confirm farmers’ preferences for grain traits using participatory rural appraisal; 2) determine amount of genetic variability for yield and yield related traits in Malawi rice landraces, 3) determine the genetic control and correlations of grain length, grain shape and 1000-grain weight, 4) determine the inheritance of aroma and gelatinization temperature and, 5) determine the genetic control, correlations and path coefficients of yield and yield related traits, in F2 generations of Malawi rice landraces and NERICA varieties crosses. To confirm the farmers’ preferences for grain traits, a participatory rural appraisal was conducted in 2006 in two villages that were representative of rainfed rice growing areas in Malawi. The villages were Liundi and Nawanga in Machinga and Salima Districts, respectively. Qualitative and quantitative data were collected through questionnaires and discussions with 190 respondents, as well as through observations. To determine variability among Malawi rice landraces, 19 landraces were planted at Lifuwu in a Randomized Complete Block Design with three replicates in 2006. Data on plant height, days to 50% flowering, number of panicles per hill, panicle length, number of filled grains per panicle, 1000-grain weight, panicle weight, grain length and grain yield were collected and analyzed. Four Malawi rice landraces were crossed to four NERICA varieties in 2006 in a North Carolina Design II mating scheme to determine the genetic control of grain size. F1 plants were raised in 2007 and in 2008, 16 F2 populations together with their parents were planted in a Randomized Complete Block Design with three replicates at Lifuwu. Data on grain length, grain shape and 1000-grain weight were collected and analyzed. To determine the inheritance of aroma and gelatinization temperature, four Malawi rice landraces were crossed to four NERICA varieties in 2006 and F1 plants were raised in 2007. In 2008, 16 F2 populations together with their parents were planted in a Randomized Complete Block Design with three replicates at Lifuwu. Aroma and gelatinization temperature were evaluated. To determine the gene action of yield and yield related traits, four Malawi rice landraces were crossed to four NERICA varieties in 2006 in a North Carolina Design II mating scheme and F1 plants were raised in 2007. In 2008, 16 F2 populations together with their parents were planted in a Randomized Complete Block Design with three replicates at Lifuwu. Data on grain yield, the number of panicles per hill, days to 50% flowering, panicle length, panicle weight and 1000-grain weight were collected and analyzed The participatory rural appraisal confirmed that that long, slender or medium shape grains, with aroma and intermediate gelatinization temperature were the key traits preferred by farmers. Therefore the farmer preferred traits of long, slender grains, with aroma and medium gelatinization temperature, must be selected for in any high yielding varieties to be developed for the rainfed rice ecosystem. Results showed that differences were significant (P=0.05) for all the traits that were studied. Heritability estimates were low to moderate: 18.3% for panicle weight, 40.0% for panicles per hill and 56.3% for days to 50% flowering date. The high genetic variability among the landraces could be used in a breeding programme to develop improved varieties for various morphological traits. The number of panicles per hill and 1000-grain weight combined moderate heritabilities with relatively high genetic advance and therefore could be reliable traits for yield improvement. Genetic analysis of grain size showed that Malawi rice landraces were variable for all three grain size characteristics, namely grain length, grain shape and 1000-grain weight. NERICA varieties were variable for 1000-grain weight. Sixteen F2 progenies were variable for all three characteristics, and the variability was significant (P=0.05). Heritability estimates were high (45.4%) for grain length and low for grain shape (12.3%) and for 1000-grain weight (14.3%) suggesting that early generation selection would be effective for grain length. Predominance of additive gene action for grain length and grain shape suggested that early generation selection would be effective for these traits. Selection for 1000-grain weight would be more effective in later generations because of preponderance of non-additive gene action in the control of this trait. The correlation between grain length and grain shape was positive (r=0.769) and highly significant (P=0.01) suggesting that breeders would choose to select for both traits simultaneously, or they would choose one of the traits to develop varieties with long grains and medium shape. Crosses between aromatic and non-aromatic varieties had non-aromatic F1. The F2 progenies segregated into 3:1 ratio for non-aromatic: aromatic suggesting that, in the Malawi rice landraces, aroma was probably simply inherited through a single recessive gene. F1 progenies, between parents with high and intermediate gelatinization temperatures had intermediate gelatinization temperature. F2 progenies segregated into 1:3 ratios for high; intermediate gelatinization temperature in three out of four crosses suggesting control by one dominant gene. The segregation pattern in one cross was not significantly different from 3:13 ratio for high: intermediate suggesting that two dominant genes, one an inhibitor, were controlling the trait. Breeding and selecting for aroma and intermediate gelatinization temperature could be accomplished relatively easily because the traits are simply inherited. The genetic variability for yield and yield related traits was wide and significant (P=0.05) in the F2 populations of Malawi rice landraces and NERICA varieties crosses indicating that the populations would be valuable sources to develop varieties with improved yield. Panicle weight and the number of panicles per hill were positively correlated with, and had high direct effects on grain yield, therefore they could be used to indirectly select for high yield. Grain yield, the number of panicles per hill and plant height were predominantly controlled by additive gene action suggesting that bulk breeding methods would be adopted for these traits. The days to 50% flowering, panicle weight and 1000-grain weight were predominantly under the control of nonadditive gene action suggesting that hybrid development would be profitable for these traits. Faya Mpata, Faya Zidyana and NERICA 3 could be the best parents for improving yield and yield related traits because they had high general combining abilities for the traits. / Thesis (Ph.D.)-University of KwaZulu-Natal, Pietermaritzburg, 2010. / Thesis (Ph.D.)-University of KwaZulu-Natal, Pietermaritzburg, 2010.

The African Rice Gall Midge (AfRGM), Orseolia oryzivora Harris and Gagné (Diptera: Cecidomyiidae), is an endemic rice pest found throughout Africa. The failure of most other control methods imposes the need to use crop resistance. This study was initiated: (1) to develop an accurate method for assessing damage caused by AfRGM; (2) to determine AfRGM resistance genes’ modes of action, the heritability estimates of their resistance to AfRGM and the behavioural pattern of progenies with resistance to AfRGM attack; (3) to reveal convergent evolution of same or similar resistance gene(s) in geographically distinct landraces, or divergent evolution of genotypes carrying the same gene, by analysing the genetic diversity among five AfRGM parental lines; (4) to build a core sample of progenies to be used as a reduced mapping population, largely reflecting the entire genome of the whole population, after an estimate of the heritability of 15 agro-morphological descriptors and; (5) determine Simple Sequence Repeat (SSR) markers flanking genes or quantitative trait loci (QTLs) linked to resistance to AfRGM. A method of accurately assessing damage caused by AfRGM was determined by comparing four methods of assessment including the International Rice Research Institute’s (IRRI) Standard Evaluation System (SES) for rice and three methods based on resistance index (RI) assessments differing in the computing of the percentage of tillers with galls on a resistant check variety. The RI-based assessment (RI-BA) methods consistently provided a better evaluation of AfRGM damage than the SES, regardless of the trial size. Within RI-BA methods, RI-BA2 was always more accurate than RI-BA1 and RI-BA3 when the plot was large. RI-BA2 and RI-BA3 were equally accurate when the plot size was small, and they provided better estimates than RI-BA1. When the plot was of medium size, RI-BA2 was more accurate than RI-BA3; RI-BA3 also surpassed RI-BA1. Overall, the best method of assessing AfRGM damage was RI-BA2, regardless of the plot size. Five rice populations including F1, F2 and F3 generations involving ITA306, a susceptible variety of Oryza sativa subsp. indica, and four varieties having different reactions against AfRGM were used to determine the genetic basis of resistance and estimate the heritability of resistance to AfRGM. All the F1s were susceptible, suggesting recessive gene inheritance. The F2 generations’ segregation pattern of 1R:15S in both ITA306-TOS14519 and ITA306-TOG7106 crosses as well as the segregation of 1R:8Seg:7S in ITA306-TOS7106 F3 families indicated that the AfRGM resistance expression being studied is governed by two genes. The deviation of the segregation patterns of crosses involving ITA306 and the tolerant parental lines from Mendelian segregation ratios suggests that the tolerance to AfRGM shown by BW348-1 and Cisadane is under complex mechanisms of control rather than under simple genetic control. The narrow-sense heritability estimates of resistance to AfRGM were low in populations involving tolerant varieties and were high in populations involving resistant varieties. They ranged from 0.086 in the ITA306-Cisadane population, to 0.4 in the ITA306-TOG7106 population. Conversely, the broad-sense heritability estimates ranged from 0.23 (ITA306-Cisadane) to 0.63 (ITA306-TOS14519). The behavioural patterns of progenies against AfRGM attack were evaluated for 532, 413 and 479 F2 progenies from ITA306-BW348-1, ITA306-Cisadane and ITA306-TOS14519 crosses, respectively, in addition to 90 BC1F2 progenies from the ITA306 and TOG7106 cross. One F3 generation of 649 families from a cross between ITA306 and TOS14519 was also tested. Four types of behavioural pattern categories were observed: (1) progenies were more resistant than the resistant check entry at 45 DAT and 70 DAT; (2) progenies were more resistant at 45 DAT and became susceptible at 70 DAT; (3) progenies were susceptible at both 45 DAT and 70 DAT; (4) progenies were susceptible at 45 DAT but reverted to resistant at 70 DAT. The first three categories were the most frequently observed and occurred in all cross combinations. The last category was observed only for a few progenies from the ITA306-TOS14519 F2 and F3 generations and, surprisingly, many from the ITA306 and BW348-1 cross. Heritability estimates were calculated for 15 major traits in an F3 population in order to predict the genetic gain associated with each trait, together with the resistance to AfRGM and to estimate the influence of the environment on phenotypic values. Broad-sense heritability (H2) estimates were high for the penultimate leaf length (PLL) - 0.99, penultimate leaf width (PLW) – 1.0, flag leaf length (FLL) - 0.99, flag leaf width (FLW) – 1.0, ligule length (LigL) - 0.99, tillering ability (Til) - 0.99, number of days to booting (DB) - 0.95, number of days to first heading (DFH) - 0.96, number of days to heading (DH) - 0.89, number of days to maturity (DM) - 0.98, culm length (CL) - 0.99, plant height (PH) - 0.99, panicle length (PanL) - 0.95, secondary branching (SB) - 0.95 and the thousand grains weight (TGW) - 0.71. Conversely, narrow-sense heritability estimates were very low (nearly 0) in PLL, FLL, Lig, DB, DFH, DM and SB or low (at most 0.267) in PLW, FLW, DH and PH, with a high value of 0.727 for TGW. Inheritance of the traits studied was therefore under non-additive gene effects rather than additive genetic effects and can therefore be improved using pedigree breeding schemes along with breeding for AfRGM resistance. Fine genetic evaluation of five AfRGM parental lines was studied in terms of polymorphisms using 303 SSR primers covering the rice genome. Of the 178 polymorphic primers identified, 60 were highly polymorphic and informative. The number of alleles amplified by these primers ranged from one to five for a total of 1,041 alleles. The polymorphism rate was globally high, ranging from 45.2% to 66.8%. The mean of the polymorphism information content (PIC) was 0.553. Factorial analysis, based on the allelic diversity, demarcated the parental lines into Oryza glaberrima Steud, Oryza sativa subsp. japonica and O. sativa subsp. indica groups, while a cluster analysis distinguished them into four groups: AfRGM resistant, susceptible, moderately resistant and tolerant. BW348-1 and Cisadane showed the least diversity, despite their distant geographical origins. TOS14519 and TOG7106 showed more divergence to ITA306 despite their common West African origin. This variability amongst the genotypes tested is the result of farmer-based selection for AfRGM resistance rather than direct breeding efforts through breeder intervention. A method of selecting individuals for a mapping population, based on a core sample, was developed in order to speed up the mapping procedure. A diversity study amongst F2 and F3 generations involving 15 quantitative and 26 qualitative agro-morphological characters was carried out and led to the dropping of seven non-discriminant descriptors. The diversity index (H) was calculated for each remaining character and the discriminant descriptors were selected based on a diversity index threshold value above 0.4. Four descriptors of H values less than 0.35 were therefore dropped. The sizing of the core collection of 64 individuals and the selection of these individuals were done using MSTRAT version 4.1 package in redundancy mode, a construction run of 100 times with an iteration number of 500. The core sample was similar to the whole population for clustering pattern, minimum and maximum quantitative values and diversity index, while mean values and coefficient of variation distinguished them. The core sample, which represents 10% of the whole population, also revealed the same phenotypic variation and the same genotypic segregation according to two SSR markers. It can therefore efficiently reflect the whole population as a mapping population. Finally, a study was undertaken to identify flanking markers to the gene/QTL involved in the resistance against AfRGM using bulked segregant analysis (BSA). A polymorphism study between ITA306 and TOS14519 displayed 145 polymorphic SSR markers, which were used to screen the bulks that originated from the two tails, and depicted only two SSRs as candidate markers linked to gall midge resistance. These markers included RM317 and RM17303 which displayed strong significance after an analysis of variance using an F test, meaning that they were segregating with the resistant alleles. / Thesis (Ph.D.)-University of KwaZulu-Natal, Pietermaritzburg, 2012.

Made available in DSpace on 2016-04-26T19:39:29Z (GMT). No. of bitstreams: 1 2007-Andre Marques dos Santos.pdf: 1289315 bytes, checksum: 28a3126a64acf0f1d9ab8338c8a0ac19 (MD5) Previous issue date: 2007-02-27 / Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior / Rice is one of the most important cereals for the humanity, because it is primary source of nutrition for more than half of the world s population. Nitrogen is the most important element for plants and is predominantly present in aerated soils in the nitrate form. As a result of the poor quality of the cultivated areas, the usage of nitrogen fertilizers intensified, contributing to the increase of pollution, since this nutrient has high mobility in the soil. With the objective of evaluating physiologic, biochemical and molecular mechanisms responsible for the efficiency of the application of nitrogen in rice, experiments were conducted at the Soils Department of Federal Rural University of Rio de Janeiro. Two rice varieties were used, Piaui (traditional rice) and IAC-47 (improved rice). In the beginning, both varieties were cultivated in nutrition solutions and submitted to treatments simulating the seasonal fluctuation of nitrate, characteristic of the tropical region. Under this condition an evaluation was done in order to define the soluble sugar levels, nitrogen fractions, and the Nitrate Reductase Activity (NRA) as well as Glutamine Synthetase Activity. Piaui rice was more efficient in the acquisition and usage of nitrogen compared to IAC-47, accumulating nitrate in its tissue during the initial phase of growth for future consumption. Later, these same rice varieties were cultivated in a growth chamber with the objective of studying the effect of increased nitrate concentrations over nitrogen fraction levels, NRA, relative expression of genes for nitrate transporters (NRT1 and NRT2) as well as a nitrate reductase (Nia2). From the results obtained it was observed that the traditional rice variety contained less NRA, higher levels of nitrogen and free amino-N. Further, traditional variety presented higher relative expression of the genes which codify nitrate transporters of low affinity (NRT1) and those of high affinity (NRT2). These plants seemed to adopt a differentiated control of NRA, because even having a higher relative expression of the genes, the NRA maintained practically without influence from the different concentrations of nitrate in the solution. In the last experiment, genes in the tips of rice roots were identified, induced by different concentrations of nitrate (0.1 and 10 mM). Several genes were sequenced, were the most important data was the subunit E1 of the dehydrogenase pyruvate complex, and phosphofructokinase, suggesting the need for a better understanding of the complexity of the interaction between carbon and nitrogen metabolism. There were also found sequences homologous to the alpha actin, myosin, tropomyosin 1, creatin kinase, and glyoxalase I genes. / O arroz ? um dos mais importantes cereais para o homem, porque ? fonte prim?ria na alimenta??o de mais da metade da popula??o do mundo. As plantas necessitam de nitrog?nio para seu desenvolvimento, sendo este presente em solos aerados, na forma de nitrato. Em fun??o do desgaste das ?reas cultivadas, tem sido intensificada a utiliza??o de fertilizantes nitrogenados, o que contribui para o aumento da polui??o, visto que, esse composto possui grande mobilidade no solo. Objetivando-se avaliar os mecanismos fisiol?gicos, bioqu?micos e moleculares respons?veis pela efici?ncia no uso de nitrog?nio em arroz foram conduzidos tr?s experimentos no Departamento de Solos da Universidade Federal Rural do Rio de Janeiro, com duas variedades de arroz, uma tradicional (Piau?) e outra melhorada (IAC-47). Primeiramente, ambas as variedades foram cultivadas em solu??o nutritiva e submetidas a tratamentos simulando o fluxo sazonal de nitrato, caracter?stico das regi?es tropicais. Nessa condi??o, foram avaliados os teores de a??cares sol?veis, fra??es nitrogenadas e a atividade das enzimas Nitrato Redutase (ANR) e Glutamina Sintetase. A variedade Piau? foi mais eficiente na aquisi??o e uso do N quando comparada a IAC-47, acumulando nitrato em seus tecidos durante as fases iniciais de crescimento. Depois, essas mesmas variedades foram cultivadas em c?mara de crescimento para estudar o efeito de concentra??es crescentes de nitrato sobre os teores das fra??es nitrogenadas, ANR e sobre o perfil de express?o relativa dos genes dos transportadores de nitrato (NRT1 e NRT2) e da Nitrato Redutase (Nia2). Dos resultados obtidos, foi observado que a variedade tradicional possui menor ANR, maiores teores de nitrato e N-amino livre. Al?m disso, esta apresentou maior express?o relativa dos genes que codificam os transportadores de nitrato de baixa (NRT1) e os de alta (NRT2) afinidade, sendo esse na parte a?rea. Estas plantas pareceram adotar um controle diferenciado da ANR, pois mesmo tendo maior express?o relativa desse gene, a atividade desta enzima se mant?m praticamente sem sofrer influ?ncia das concentra??es de nitrato na solu??o. No ?ltimo experimento, foram identificados os genes induzidos por duas concentra??es de nitrato (0,1 e 10 mM) em ?pices radiculares de arroz. Diversos genes foram seq?enciados, dentre eles, destacou-se a subunidade E1 do complexo piruvato desidrogenase e fosfofrutoquinase, indicando a necessidade de se melhor entender a complexidade da intera??o entre o metabolismo de carbono e nitrog?nio. Foram encontradas tamb?m seq??ncias hom?logas aos genes da alfa actina, miosina, tropomiosina 1, creatina cinase e glioxalase I.

Genetic resources are important for the development of every country and for humanity. Collection, conservation and reasonable utilization of genetic resource is required mission. Understanding the importance of genetic resource, especially rice germplasm, since 2001, Center for conservation and development of crop genetic resources (CCD-CGR) of Hanoi University of Agriculture (Vietnam National University of Agriculture) has been collected, conserved and evaluated rice germplasm from different provinces of Vietnam for breeding programs. So far, 1090 accessions of local rice of Vietnam have been collected. Evaluation of agronomic properties and screening of some important genes using DNA molecular markers have revealed that Vietnamese rice germplasm has high level diversity and containing important genes for quality and resistance for disease and pests. These genetic resources are potential materials for national breeding programs. Based on the collected germplasm, 3 new glutinous rice varieties have been successfully created with high yield and good quality. In addition, the degradation of local rice varieties is also a matter of concern. So far, 4 specialty rice varieties Deo Dang, Ble chau, Pu de and Khau dao have been successfully restored for the north provinces of Vietnam. The main results of this study are germplasms for rice breeding programs and new improved varieties that bring economic benefits to farmers and the country. / Nguồn gene là tài nguyên sống còn của mỗi quốc gia và của toàn nhân loại. Vì vậy thu thập, bảo tồn, đánh giá và khai thác hợp lý nguồn tài nguyên này có ý nghĩa rất lớn. Nhận thức được tầm quan trọng của nguồn gen nhất là nguồn gen cây lúa, ngay từ đầu những năm 2000, Trung tâm bảo tồn và phát triển nguồn gene cây trồng thuộc Trường Đại học nông nghiệp, nay là Học Viện nông nghiệp Việt Nam đã tiến hành thu thập, lưu giữ, đánh giá và khai thác nguồn gene lúa. Kết quả đã thu thập, lưu giữ được 1090 mẫu giống lúa địa phương Việt Nam. Đánh giá đặc điểm nông sinh học và phát hiện một số gene quy định các tính trạng chất lượng và kháng sâu bệnh bằng chỉ thị phân tử DNA. Đây là nguồn gene quan trọng cho chọn tạo giống. Dựa vào nguồn gene thu thập được, cho đến nay, Trung tâm bảo tồn và phát triển nguồn gene cây trồng đã lai và chọn tạo được thành công 03 giống lúa nếp chất lượng cao. Ngoài ra, thoái hóa giống cũng là vấn đề đang được quan tâm. Cho đến nay 4 giống lúa đặc sản Đèo đàng, Ble châu, Pu đe và Khẩu dao đã được phục tráng và đưa vào sản xuất. Kết quả của những nghiên cứu này là ngân hàng các giống lúa làm nguồn gene để chọn tạo giống mới đem lại lợi ích kinh tế cho người nông dân và đất nước.