Cette thèse s’applique à améliorer la compréhension de l’absorption du cadmium (Cd) par le maïs et le tabouret calaminaire, dans l’objectif de mieux modéliser son transfert vers les plantes comestibles ou hyperaccumulatrices. Le modèle utilisé étant sensible aux caractéristiques d’absorption racinaire, le premier objectif était de développer une méthode rigoureuse de mesure de ces paramètres. Deux protocoles ont été mis au point pour décrire précisément, en fonction de la concentration de Cd en solution, l’influx net de Cd dans les parois et dans le milieu intracellulaire des racines. Les résultats ont mis en évidence, pour la première fois, l’existence d’un système de transport à faible affinité (LATS) qui agit en même temps que le système de transport à forte affinité pour le Cd (HATS). Les nouveaux paramètres cinétiques mesurés n’ont cependant pas amélioré significativement le modèle : le prélèvement de Cd par le maïs est surestimé de 100%, et son prélèvement par le tabouret calaminaire est sous-estimé de 66%. Plusieurs facteurs ont alors été étudiés pour comprendre les raisons de ce décalage. Nous avons montré que les conditions dans lesquelles ont été mesurés les paramètres cinétiques présentaient des caractéristiques capables de modifier radicalement leurs valeurs : la composition ionique de la solution d’exposition au Cd, la concentration de Cd durant la croissance et la structure racinaire engendrée par l’hydroponie. Cette thèse suggère de cultiver les plantes en aéroponie et de mesurer les paramètres cinétiques dans une composition ionique représentative du voisinage des parois et des membranes racinaires en sol, composition qu’il reste encore à déterminer. / This thesis aimed to improve the comprehension of cadmium (Cd) absorption by maize and alpine pennycress plants in order to model better its transfer into edible and hyperaccumulating plants. Since the model used is sensitive to the characteristics of root absorption, the first objective was to develop a rigorous method for measuring those parameters. Two protocols were finalized to describe precisely Cd net influx in both root compartments (apoplast and symplast) according to Cd concentration in solution. The results highlighted, for the first time, the existence of a low-affinity transport system (LATS) that works at the same time with the high-affinity transport system (HATS). However, the kinetics parameters measured through these experimentations did not succeed in improving significantly the model: Cd uptake by maize is overestimated by 100%, and Cd uptake by alpine pennycress is under-estimated by 66%. Several factors were investigated in order to understand the reasons of this difference. We showed that the experimental conditions used to measure the kinetics parameters present characteristics that are able to modify their values significantly: the ionic composition of the solution of exposition to Cd, Cd concentration during growth, and the root structure that forms in hydroponics. This thesis suggests to choose aeroponics as controlled culture condition, and to measure the kinetics parameters in an ionic composition that is representative of the close vicinity of the root cell walls and membranes in soil. This composition remains to be investigated.

<p>We evaluated 3-D imaging of coarse root structure and biomass using ground-penetrating radar (GPR). GPR surveys were conducted in a white pine forest in southern Ontario, Canada. GPR profiles were obtained across two test plots (6 and 17 m² area), using 1-GHz GPR and a MEMS (micro-electro-mechanical systems) accelerometer. Test plot surveys evaluated the effects of micro-topography, soil moisture content, and root diameter and spacing. In addition, with the aid of the outcome of the control test plots two other plots (25 and 400 m²area) were surveyed with varying line sample spacing to investigate the restraints on resolution brought about by line sampling density.</p> <p>Accounting for antenna tilt is necessary to determine an accurate and more precise position of root mass. The antenna tilt was >45^o pitch, >28^o roll and up to 10^o yaw due to surface micro-topography of the forest floor. Vector 3-D imaging enhanced the diffraction amplitude (15.5% increase) and centralized the position of the root. Radial surveys provided root continuity and produced better root imaging.</p> <p>GPR largely underestimates coarse root biomass when a line spacing of 25 cm is used. However similar results are found with smaller line spacing (12.5 cm). A maximum line spacing of 10 cm provided continuous root structure and differentiation of roots spaced 10 cm apart and greater. A sampling line spacing of 5 cm and an inline sampling interval of 0.5 cm in low soil moisture conditions provided the detection of roots that were a minimum of 1.4 cm in diameter.</p> / Master of Science (MSc)