Given that morphological identification of willow is difficult, willow lines being investigated for their suitability for use as short rotation crops for biomass production in Saskatchewan were investigated with various molecular techniques as possible tools for DNA fingerprinting. Flow cytometry was used to assess variation in nuclear DNA content and thus ploidy level of the lines of the five species (<i>Salix purpurea, Salix eriocephala, Salix sachalinensis, and Salix dasyclados</i>) and three hybrids (<i>S. purpurea x S. miyabeana, S. sachalinensis x S. miyabeana, S. viminalis x S. miyabeana</i>). The DNA content varied between 1.14 and 3.00pg. Ploidy levels of the species varied from triploid to hexaploid while all hybrids were tetraploid. RAPD and ISSR marker systems were used to assess genetic and taxonomic relationships among all willow lines. Of 90 RAPD primers tested, 60 were selected and 99 polymorphic bands scored. Of 35 ISSR primers tested, 19 were selected and 35 polymorphic bands scored. Both RAPD and ISSR dendrograms clustered together lines belonging to the same species and same hybrid combination. A combination of strong and reproducible RAPD and ISSR bands was used to develop identification keys for lines belonging to the same species.<p> The ribosomal RNA gene region, including the entire 5.8S RNA gene and the internal transcribed spacers (ITS1 and ITS2) was amplified and sequenced to assess sequence homology between the five species. The total length of the amplified region was 601bp, with the ITS1, 5.8 S and ITS2 being 223, 163, and 215bp respectively. Intra- and inter-species SNPs were observed, 6 within ITS1, and 3 within ITS2. No polymorphisms were found in the 5.8S gene. The low rate of variation within the sequenced ITS fragment between species supports the monophyly of the five species involved in this study, and confirms their belonging to the subgenus Caprisalix. SCAR primers were designed from species-specific polymorphic nucleotides and applied to the willow collection to test their use for species identification. A species identification key based on SNPs is proposed.

In Canada, willow (Salix spp.) short rotation intensive cultures (SRIC) have been established to investigate their potential to produce biomass for bioenergy. Since root-associated fungal communities are involved in plant nutrition and disease susceptibility, it is relevant to understand their interactions with willows and their role on the sustainability of SRIC. In this project traditional methods together with molecular techniques were used to: i) assess the diversity of fungal communities in roots of willows and their relationship with major characteristics of SRIC ii) evaluate the effects on plant development of potentially beneficial and pathogenic fungi, and iii) identify arbuscular mycorrhizal fungal (AMF) species and their interaction with promising Salix clones. Potentially pathogenic fungi were more dominant in diseased and recently planted cuttings than in healthy older ones. This suggested the occurrence of a positive shift prompted by the establishment of the cuttings on the site. Gibberella/Fusarium sp. and Neonectria sp. were the most dominant taxa particularly in diseased plants. Under greenhouse conditions the inoculation with potentially beneficial and potentially pathogenic fungi induced significant differences in root biomass but not in overall aerial biomass production. PCR-denaturing gradient gel electrophoresis was successfully standardized and used to identify arbuscular mycorrhizal fungal species associated with different clones. The results indicated a degree of specificity between AMF species and plant genotype. Root-associated fungal communities appear to be useful to monitor the impacts of SRIC on soil ecology and their study may enlighten effective ways to increase the productivity of these biomass systems.

No description available.

Abstract   The aim of this study was to increase knowledge of how plants as a whole respond to targeted fertilization and to refine the method of foliar nutrition fertilization. The experiment also aimed to test how herbivores respond when presented with a choice between fertilized and unfertilized Salix leaves in a feeding trial experiment. One major problem that arises when trying to alter the nutrient levels within a specific part of a plant is that adding fertilizer directly to the soil in which the plants grow will have a systemic effect on the plant, causing the plant physiology as a whole to change instead of just changing the properties of the plant part which the study focuses on. The approach chosen for this experiment is to target the leaves directly when applying the fertilizer and therefore try to avoid altering the rest of the plant. Fertilizer in the form of NH3NO4 was added to Salix leaves using a pipette and a brush at two different concentrations. When the leaves had been fertilized they were analyzed and used in a feeding trial experiment to study herbivore response to targeted fertilization. Once all data was analyzed statistically it became clear that both concentrations of the treatment had altered the nitrogen content within the leaves, however only the lower concentration had done so without altering the characteristics of the leaf. The data collected from the feeding trial displayed no statistical evidence for preference between the treated and control leaves in regard to amount consumed by the Phratora beetles, on the other hand when testing for egg laying preference 8/8 of egg clutches were laid on treated leaves.

Diss. (sammanfattning). Umeå : Sveriges lantbruksuniv., 2003. / Härtill 4 uppsatser.

Diss. (sammanfattning). Uppsala : Sveriges lantbruksuniv., 2003. / Härtill 4 uppsatser.

Lic.-avh. Uppsala : Sveriges lantbruksuniv. / Härtill 2 uppsatser.

Thesis (doctoral)--Swedish University of Agricultural Sciences, 2000.

This thesis deals with the burden of European beaver (Castor fiber) populations with risk elements in three areas of the Czech Republic and one area in Slovakia. There were investigated concentrations of heavy metals (Cd, Zn, Fe, Cu, Pb and As) in the kidneys, livers, lungs, hearts, muscles and fur of 19 found dead beaver individuals. Furthermore, their food was analysed to determine the contamination of their areas of origin and discover, which organ reflects the observed concentrations of Cd the best. The main aim of the study was to determine whether the analysis of hair could be used as a non-invasive method to reflect the concentrations of heavy metals in beaver populations in future. The results were conclusive only for As, whose concentrations in the hair was the second highest and correlated with the content of As in the lungs, which contained the highest concentrations of arsenic. Cadmium in the fur showed a reverse structure compared to the content of Cd in kidney and liver. There were found significant differences in the contamination with heavy metals between beaver populations and their environments. Analysis of the fur is not proved for most of the elements and it is necessary to investigate further non-invasive methods to determine the load of toxic elements on beaver populations.

No description available.