The aim of this dissertation was to compare qualitative and quantitative of a Sauvignon clone samples, describe and evaluate particular phenological phases and uvology values, describe and evaluate the analysis of must. The experiment was established in the viticulture Vineyards -- Hnanice. On three variants of Sauvignon clones differences in phenological phases in the same conditions were observed. Subsequently analytical values of must from each clone were evaluated. The sugar content, titratable acids, the pH, the yield per a bush, the weight of 50 berries, the average weight of a bunch of grapes, the average weight of a berry, the yield of grapes per a bush were monitored. The results showed that the Austrian A clone that has been the weakest in a growth, had the highest values, and vice versa French clone F 316 with the most extravagant growth had the lowest values. The results showed that it is advisable to plant more clones with different properties. The results were statistically analyzed and compared with attempts by other authors.

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The impact of the term of defoliation of the zone of grape on quality and condition of plant health for the Sauvignon blanc variety The focus of the Thesis is to evaluate the effect of the term of defoliation of the zone of grape on quality and condition of plant health for Sauvignon blanc variety. The introduction of the theoretical part briefly characterizes the issue of leaf area -- factors affecting it. The next sections describe the green work and the attention is focused primarily on defoliation of the zone of grape -- the impact, the terms and the intensity of defoliation. The factors determining the ripeness and quality of grapes are briefly described as well individual qualitative parameters. The practical section describes he methodology used in the evaluation of the experiment of defoliation on four different terms and the results of the examined variants of defoliation are interpreted.

The purpose of the thesis on topic Effect of date of defoliation on aromatic profile ´Sauvignon blanc´ wine was to find out the effect of removing leaves from grapes zone on qualitative and quantitative parameter of grapes of this species. The practical part of the experiment with defoliation in different terms was carried out during 2015 on a vineyard in Svatobořice-Mistřín. Part of the practical experiment was the production of wines from different variants and and evaluated the sensory properties of wine.

Memoria para optar al titulo Profesional de Ingeniero Agrónomo Mención: Enología y Vitivinicultura

Memoria para optar al Título Profesional de I ngeniero Agrónomo Mención: Manejo de Suelos y Aguas / En la temporada 2001 – 2002, se realizó un estudio del efecto del crecimiento vegetativo y carga frutal, en la variedad Cabernet Sauvignon, en el Valle del Maipo. Se evaluó el efecto de la carga frutal sobre la producción y calidad del mosto en tres ensayos, fertilizados con 20, 40 y 60 kg N•ha-1 respectivamente. La carga frutal fue regulada a inicios de envero, y los tratamientos fueron 10, 14 y 18 racimos por planta. Adicionalmente, se estudió el efecto del crecimiento vegetativo sobre la producción, susceptibilidad a plagas y enfermedades, y calidad del mosto, en dos sitios caracterizados como de alto y bajo vigor. En los ensayos de carga frutal, hubo un efecto significativo en el rendimiento al existir una diferencia de 8 racimos menos por planta, y no hubo diferencias en el contenido de azúcar y el tamaño de las bayas, al comparar entre las cargas frutales. La calidad del mosto sólo se vio afectada cuando la relación entre la producción y el crecimiento vegetativo (índice de Ravaz) aumentó significativamente y se elevó sobre 10, al ajustar la carga frutal “alta” (18 racimos por planta) en una condición de fertilización moderada (40 kg N•ha-1). En esa situación, el contenido de taninos aumentó significativamente lo que puede afectar en forma negativa la calidad del mosto. En plantas vigorosas se observó un menor número y peso promedio de racimos, y una mayor incidencia de oídio y palo negro, en comparación con plantas de menor vigor. La calidad del mosto obtenido fue comparativamente menor. De acuerdo a la experiencia generada en esta investigación, y en otras realizadas en el mismo sector, se ha determinado que el estudio del efecto del crecimiento vegetativo y la carga frutal sobre el comportamiento del viñedo debe considerar paralelamente y en detalle el estado hídrico de las plantas.

This diploma thesis compares the differences between clones of the Sauvignon variety, which are planted in the Central Slovakia wine region in the village of Veľký Krtíš and then evaluates the results of this comparison. Three clones from French and one clone from Tyrol are planted together in one vineyard track. The literary part of this thesis describes general information about Sauvignon variety, its clones and aromatic substances. In the vineyard were studied the phenological phases of the clones, uvological values and yield. From each clone has been made wine separately. For each wine were determined basic parameters and difference of aromatic substances using method of comprehensive two-dimensional gas chromatography. Based on the results of the expert committee were created the aromagrams and the position of the wines in the hundred-point table according to standards of the O.I.V.

The thesis deals with the issue of defoliation after quality grapes. The work deals with the comparison of mechanical and manual defoliation. The first part deals with searching and displaying information with this issue. The second part is about my experiment, its processing and its own recommendations. I was able to do this experiment on the Plaček vineyard in Moravské Branice where I used 11 lines of Sauvignon blanc variety for the experiment when every line indicates another experiment variation. Defoliation zone of grapes took place since 7.6.2018. Manual defoliation always removed 1-3 leafs and other part from defoliation zone. Since 15.6.2018 we used a mechanized defoliation using the defoliator. The following experimental variations were made in the same terms both manually and mechanically. The last mechanical defoliation was on 1.7.2018 and we performed a defoliation variant as a control. The last attempts were made between 26.7 and 2.8.2018 where we used another method of defoliation zone of grapes for a purpose to slown down the ripening. During the ripening of the grapes their health and quality was monitored. In four weeks carried out the evaluation of qualitative grapes. The berry sugar content, Ph value, acid content, nitrogen content and other defoliation-related parameters were measured. All measurements were taking place at a school laboratory in Lednice. All data have been evaluated and we written own recommendations for winemakers and vineyard oweners.

The use of the electronic nose in the wine industry is an emerging technology. Although electronic nose systems have been used in other industries as tools to monitor fruit maturity or quality control, the use of these systems for wine analysis has been debated. Many electronic nose sensor types exist, but have often been accused of primarily discriminating varying ethanol concentrations of wines as opposed to aroma and flavor volatiles. This study evaluated the use of a commercial conducting polymer electronic nose to evaluate wines while minimizing ethanol interference. The first study presented evaluated the discrimination ability of an electronic nose with use of an ethanol baseline prior to wine evaluation. This experiment also determined the optimal wine temperature for electronic nose analysis. A second study reviewed the ability of the electronic nose to discriminate Cabernet Sauvignon grape and wine volatiles based on a pre-fermentation cold soak treatment. The electronic nose was used to monitor volatile changes throughout a five day cold soak, and to discriminate differences in control versus cold soak wines. These results were compared with juice and wine analytical data, GC-MS evaluation of individual volatiles, and sensory analysis of the finished wines. It was found that discrimination of wines improved from 33% to 60% when using an ethanol baseline. Influence of the baseline was indicated by a drop in sensor response prior to wine evaluation. A 30°C sample temperature was chosen for wine analysis based on sensor response and adequate discrimination in canonical distributions. The electronic nose was found to discriminate grape volatiles in cold soak musts as shown in canonical distributions at a 95% significance level. PCA distributions of electronic nose data, chemistry data, and GC-MS data showed varying degrees of discrimination based on analysis. Electronic nose data often showed 100% of variation of samples accounted by PC1. Despite differences in treatment by ENose and analytical data, sensory results did not find a difference in control and cold soak wines. / Master of Science

Grape-derived aroma and flavor precursors exist partially as non-volatile, sugar-bound glycosides. Hydrolysis of these compounds may modify sensory attributes and potentially enhance wine quality. Cold soak (prefermentation skin contact) at two temperatures and alcohol content (%, v/v) at dejuicing were monitored to determine effects on Cabernet Sauvignon glycoside concentration. Total, phenolic-free, and red-free glycoside concentrations were estimated by the quantification of glycosyl-glucose. Cold soak (5 days at 10° C) increased total glycosides by 77%, red-free glycosides by 80%, and phenolic-free glycosides by 96%. Ambient soak (3 days at 20° C) enhanced color extraction, and increased total glycosides by 177%, red-free glycosides by 144%, and phenolic-free glycosides by 106%. Wines produced by early pressing (10% sugar) had 25% more total and red-free glycosides than late press (0.25% sugar). After post-fermentation malolactic fermentation, total glycosides were 14% lower and phenolic-free glycosides were 35% lower. In a second study, the activities of a-L-arabinofuranosidase, b-glucosidase, and a-L-rhamnoyranosidase were determined in model systems for thirty-two strains of yeasts belonging to the following genera: Aureobasidium, Candida, Cryptococcus, Hanseniaspora, Hansenula, Kloeckera, Metschnikowia, Pichia, Saccharomyces, Torulaspora, and Brettanomyces (10 strains); and seven bacteria (Leuconostoc oenos strains). Only one Saccharomyces strain exhibited -glucosidase activity, but several non-Saccharomyces yeast species had substantial production. Aureobasidium pullulans hydrolyzed a-L-arabinofuranoside, b-glucoside, and a-L-rhamnoyranoside. Eight Brettanomyces strains had -glucosidase activity. Location of enzyme activity was determined for those species with enzymatic activity. The majority of -glucosidase was located in the whole cell fraction (66%), followed by the permeabilized fraction (35%), and extracellular production (2%). Aureobasidium pullulans was also capable of hydrolyzing grape glycosides. / Master of Science