The wild populations of the Scarlet Macaw subspecies native to southern Mexico and Central America, A. m. cyanoptera, have been drastically reduced over the last half century and are now a major concern to local governments and conservation groups. Programs to rebuild these local populations using captive bred specimens must be careful to reintroduce the native A. m. cyanoptera, as opposed to the South American nominate subspecies (A. m. macao) or hybrids of the two subspecies. Molecular markers for comparative genomic analyses are needed for definitive differentiation. Here I describe the isolation and sequence analysis of multiple loci from 7 pedigreed A. m. macao and 14 pedigreed A. m. cyanoptera specimens. The loci analyzed include the 18S rDNA genes, the complete mitogenome as well as intronic regions of selected autosomally-encoded genes. Although the multicopy18S gene sequences exhibited 10% polymorphism within all A. macao genomes, no differences were observed between any of the 21 birds whose genomes were studied. In contrast, numerous polymorphic sites were observed throughout the 16,993 bp mitochondrial genomes of both subspecies. Although much of the polymorphism was observed in the genomes of both subspecies, subspecies-specific alleles were observed at a number of mitochondrial loci, including 12S, 16S, CO2 and ND3. Evidence of possible subspecies-specific alleles were also found in three of four screened nuclear loci. Collectively, these mitochondrial and nuclear loci can be used as the basis to distinguish A. m. cyanoptera from the nominate subspecies, A. m. macao, as well as identify many hybrids, and most importantly will contribute to further reintroduction efforts.

abstract: Exchange is fundamental to the establishment and maintenance of social institutions and political economies in all scales of societies. While today people rapidly exchange goods and information over great distances, in the past, long-distance exchange necessitated the mobilization of vast networks of interaction with substantial transport costs. Objects traded over long distances were often valuable and challenging to obtain, granting them multifaceted significance that is difficult to understand using traditional archaeological approaches. This research examines human interactions with scarlet macaws (Ara macao) in the United States (U.S.) Southwest and Mexican Northwest (SW/NW) between 900 and 1450 CE. This period saw large-scale cultural change in the form of migrations, rapid population aggregation, and an expansion of long-distance exchange relations in regional centers at Pueblo Bonito (900-1150 CE) in northwestern New Mexico, Wupatki (1085-1220 CE) in north-central Arizona, and Paquimé (1200-1450 CE) in northern Chihuahua. Despite the distant natural habitat of scarlet macaws, their importation, exchange, and sacrifice appear to have played integral roles in the process of placemaking at these three regional centers. Here, I use an Archaeology of the Human Experience approach and combine radiogenic strontium isotope analysis with detailed contextual analyses using a Material Histories theoretical framework to (1) discern whether macaws discovered in the SW/NW were imported or raised locally, (2) characterize the acquisition, treatment and deposition of macaws at Pueblo Bonito, Wupatki, and Paquimé, and (3) identify patterns of continuity or change in acquisition and deposition of macaws over time and across space in the SW/NW. Findings from radiogenic strontium isotope analysis indicate that scarlet macaws from all case studies were primarily raised locally in the SW/NW, though at Paquimé, macaws were procured from sites in the Casas Grandes region and extra-regionally. Variation in the treatment and deposition of scarlet macaws suggests that despite their prevalence, macaws were interpreted and interacted with in distinctly local ways. Examination of the human experience of transporting and raising macaws reveals previously unconsidered challenges for keeping macaws. Overall, variation in the acquisition and deposition of scarlet macaws indicates changing strategies for placemaking in the SW/NW between 900 and 1450 CE. / Dissertation/Thesis / Doctoral Dissertation Anthropology 2020

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