This Masters in Art and Design investigates a performance strategy for the deterritorialization of the body, mind and space as they intersect the digital screen image. The reconfiguration of the sensing system as the body encounters the digital image deterritorializes the body’s everyday modes of proprioception and spatial orientation. The assumption here is the increasing 'instability' of the body in a contemporary world where the digital screen image mediates and renegotiates our physical encounters. In prioritizing the body in these screen environments, there is potential for rethinking a body politic for performance and somatic practice. My strategy is to reconfigure the multi-modal processing where the screen dominates the visual faculties in the mind/body/screen relationship. Using wireless cameras attached to the body and improvisational structures for performance, divergent spaces are connected and collected through the body, screen and the camera. As the body and screen intersect, the corporeal and the image converge and manifest through the imagination and screen. This investigation into the screen/body opens up new possibilities for the spatial and corporeal, as the body and the screen fold into a mesh of multiplicity and 'in-between-ness'.

Thesis (M.S.)--Rochester Institute of Technology, 1987. / Typescript. Includes bibliographical references (leaves 43-44).

Thesis (M.S.)--Rochester Institute of Technology, 1994. / Typescript. Includes bibliographical references (leaves 77-81).

Thesis (M.S.)--Rochester Institute of Technology, 1993. / Typescript. Includes bibliographical references (leaves 91-93).

BACKGROUND Routine renal screens are essential to the healthcare maintenance of pediatric patients with sickle cell disease. There is an inadequate amount of evidence, however, to support a universal standardized practice. As a result, sickle cell patients are subject to significant variability in care. The objective of this study was to assess the practice of routine renal screens at Boston Children's Hospital. METHODS We performed a chart review of 330 total patients from 2011 through 2013 to analyze the performance of microalbumin renal screens and urinalyses at routine clinic visits. With this data, we evaluated the institution's compliance with its adopted renal screening guidelines, examined renal screening variability across patient demographics and among providers, and compared the performance of different laboratory measures. RESULTS Renal screens were performed in 34.2% of the eligible study population. There were no statistically significant differences in the performance of renal screens across patient age, sickle phenotype or disease-modifying therapy. Patients aged from 5-9 years old, however, demonstrated a non-significant trend toward a lower frequency of successful renal screens. The percentage of samples collected for microalbumin excretion within the female cohort was significantly higher than the institutional average (41.7% vs 34.2%, p = 0.024) and significantly lower within the male population (27.6% vs 34.2%, p = 0.034). The performance of renal screens varied significantly from one provider to another. In all areas of analysis, the frequency of urinalyses performed was similar to the frequency of microalbumin excretion rates. CONCLUSION This study suggests that providers are not routinely adhering to the institutional standard for renal screens. There remains little insight into providers' basis of renal screen practice: providers may not be cognizant of the institution's practice guidelines or may not consider early detection of microalbuminuria essential to the healthcare maintenance in patients with sickle cell disease. Future studies are warranted to fully comprehend the low frequency of renal screens performed in pediatric sickle cell patients.

Made available in DSpace on 2014-06-11T19:24:57Z (GMT). No. of bitstreams: 0 Previous issue date: 2008-02-12Bitstream added on 2014-06-13T20:13:28Z : No. of bitstreams: 1 ghaziri_sm_me_mar.pdf: 1656666 bytes, checksum: 1c115f567a33b979fb1ae22a33a0a7d7 (MD5) / Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) / Este trabalho estuda as novas práticas e modos de leitura emergentes no contexto do ciberespaço, procurando refletir sobre as implicações dessas novas práticas para a formação do leitor na escola. Desse modo, o objetivo mais amplo da pesquisa foi o de mapear a leitura na tela do computador - conectado à Internet – entre seis sujeitos do ensino fundamental de uma escola pública do município de Assis – SP. Os sujeitos foram observados em situação de leitura no impresso e na tela, para que , entre outros pudessem ser observados comportamentos transferidos de um suporte para o outro, tanto do impresso para a tela, como da tela para o impresso. A metodologia que guiou a execução do trabalho foi o estudo etnográfico e grupo focal uma vez que era de nosso interesse a elaboração de conhecimento calcado na observação in loco de comportamento humano. Foram analisados os modos de operar o pensamento emergentes da prática de leitura no ambiente virtual, bem como sua possível relação com outras mídias Os dados coletados foram categorizados com base na análise de conteúdo e refletidos à luz de bibliografia especializada, em que se destacam como autores centrais: Chartier (1999; 2001; 2002; 2005), Eisenstein (1998), Nielsen (1997; 2006), Bakhtin (2000; 2004) e Mcluhan (1969; 1972). Foi possível verificar que a leitura na tela é rápida, como um escaneio do texto, em que o leitor parece mobilizar estratégias de antecipação e previsão de seu trajeto de navegação. Além disso, a atribuição de sentido na leitura se dá por meio de um processo de união de fragmentos de informação; a experiência hipertextual faz do leitor co-autor dos textos que lê. Por fim, é preciso dizer que alguns leitores se encaminhavam para a leitura na tela com as estratégias do impresso, o que em alguns casos comprometia o encontro das informações buscadas. / This work is characterized as a study concerning the new practical and the emergent ways of reading in the context of cyberspace, looking for to reflect on the new implications of these practical for the formation of the reader in the school. In this way, the objective amplest of the research was to characterize the reading in the screen of the computer - connected to the Internet - enter six students of the basic education of a public school of the city of Assis - SP. The students had been observed in situation of reading in the printed matter and the screen, searching, among others facts, to observe behaviors led of a support to the other (in such a way of the printed matter for the screen as of the screen for the printed matter). The methodology that guided the execution of the work was the study ethnographic of case, a time that was of our interest the elaboration of knowledge based in the comment in leases of human behavior. The collected data had been categorized on the basis of the reflected analysis of content and to the specialized bibliography light, where if they detach as authors central offices: Chartier (1999, 2001, 2002 e 2005), Eisenstein (1998), Nielsen (1997 e 2006), Bakhtin (2000 e 2004) and Mcluhan (1969 e 1972). As results, it was verified, from the actions of the showed students, that the reading in the screen is fast, an scanning of the text, where the reader must mobilize strategies of anticipation and forecast, the text and its passage of navigation. Moreover, the felt attribution of in the reading if gives by means of a process of union of fragments of information. The hipertextual experience makes of the reading co-author of the texts who reads. Finally, the ways had been analyzed emergent to operate the thought of the practical one of reading in the virtual environment, as well as its possible relation with other Medias.

Made available in DSpace on 2014-06-12T15:51:13Z (GMT). No. of bitstreams: 2 arquivo1504_1.pdf: 791063 bytes, checksum: 4a11c48c67eb0f30d0a7814f389f14a4 (MD5) license.txt: 1748 bytes, checksum: 8a4605be74aa9ea9d79846c1fba20a33 (MD5) Previous issue date: 2009 / Coordenação de Aperfeiçoamento de Pessoal de Nível Superior / A necessidade de métodos mais versáteis para a mensuração e monitoramento dos níveis séricos de lipídios tem estimulado a produção de uma grande variedade de novos métodos analíticos. O presente trabalho mostra o desenvolvimento de um biossensor monoenzimático para detecção de triglicerídeos empregando a tecnologia de Screenprinting através da qual, foram impressos os eletrodos de referência (Ag/AgCl) e o de trabalho contendo uma mistura de carbono e quitosana. A lipase foi imobilizada por adsorção no eletrodo de trabalho. A lipase solúvel, usando pNPM, apresentou pH ótimo de 8 e temperatura ótima de 32,5°C, mantendo cerca de 78,5% da atividade máxima a 25°C (temperatura operacional do biossensor). O Km e Vmáx para a lipase solúvel em relação ao pNPM foram de 0,95 mM e 25,51 U/mg proteína, respectivamente. No biossensor desenvolvido com apenas lipase imobilizada, usando trioleína como substrato, foram de 0,148 mM e 262,21 μA/s e 0,304 mM e 85,07 μA/s para a lipase solúvel e TIP sem enzima. Os resultados mostraram linearidade nas respostas de correntes para trioleína (0,25mM 2mM), atestando a viabilidade do biossensor desenvolvido, utilizando unicamente a lipase imobilizada na superfície do eletrodo de trabalho, podendo ser empregado eficientemente para a detecção de triglicerídeos

Physical inactivity and sedentary behaviour have been independently associated with a wide range of negative health indicators including obesity, poor cardio-metabolic health, and poor psychosocial health. The overarching objective of this research was to gain a better understanding as to why children are sedentary, and where we need to focus public health messages and interventions to reduce sedentary behaviour. Specifically, I aimed to provide insight on current awareness of sedentary behaviour guidelines; determine important correlates of total sedentary time (SED), and screen time (ST) in Canadian children; and understand correlates of SED and ST in a global context. The primary dataset used for this project was the International Study of Childhood Obesity, Lifestyle and the Environment (ISCOLE). Background work was completed to review current literature on knowledge and awareness of Canadian physical activity and sedentary behaviour guidelines (in all age groups), and to understand the representativeness of the ISCOLE dataset. In addition to the two background papers, this dissertation includes three manuscripts, all prepared for submission in scientific, peer-reviewed journals: 1. Manuscript 1: Canadian physical activity and screen time guidelines: do children know? 2. Manuscript 2: Correlates of objectively measured sedentary time and self-reported screen time in Canadian children 3. Manuscript 3: Correlates of total sedentary time and screen time in 9-11 year-old children around the world: The International Study of Childhood Obesity, Lifestyle and the Environment Overall, this work showed the majority of children around the world are accumulating large amounts of sedentary time, and exceeded current screen time guidelines. We found that the large majority of Canadian children are not aware of screen time guidelines; however, a greater proportion of children could identify physical activity guidelines. We also identified a number of correlates of SED, and ST in Canadian children, and in children around the world. The most common correlates included weight status, and access to electronics in the house. Taken together, this work suggests that public health messaging should focus on increasing awareness of screen time guidelines. While increasing awareness of the guidelines, messaging can be tailored to promoting healthy weight status, and reducing (or removing) children’s access to electronic devices in hopes of reducing overall time spent sedentary.

In addition to ATP generation, mitochondria are essential in various cellular processes ranging from biosynthetic pathways, apoptosis, cell cycle progression, and calcium buffering. Studies in living cells have now firmly established that mitochondria exist as a dynamic network sculpted by fission and fusion reactions, rather than separated, individual organelles. Not surprisingly, mutations in genes involved in mitochondrial dynamics and quality control lead to human diseases such as Charcot-Marie-Tooth disease type 2A, Optic atrophy, and autosomal recessive Parkinson disease. I have designed a high-throughput protocol to permit genome-wide screening for novel genes that are required for normal mitochondrial morphology. I have executed a genome-wide RNA interference screen and identified several novel genes required for mitochondrial dynamics in addition to known regulators of mitochondrial dynamics. A detailed high-throughput genome-wide screening protocol is presented. I have shown that TID1, a gene identified from the screen, has a dual-role in maintaining the integrity of mitochondrial DNA and preventing the aggregation of complex I subunits. My analysis of the mitochondrial role of TID1 supports the existence of a TID1- mediated stress response to ATP synthase inhibition. The genome screen also identified the novel gene ROMO1 as essential for normal mitochondrial morphology. I have shown that ROMO1 may have an additional role in maintaining mitochondrial spare respiratory capacity, possibly by affecting cellular substrate availability. Finally, in a collaborative effort, we have shown that homozygous mutations in the mitochondrial fusion gene MFN2 lead to multiple symmetric lipomatosis (MSL) associated with neuropathy. Mechanistically, this mutation reduces MFN2 homocomplex formation. Taken together, these results show the utility of genome-wide screening in identifying genes involved in mitochondrial quality control.

Parkinson’s disease (PD) is a progressive neurodegenerative movement disorder characterized by the loss of dopaminergic (DA) neurons in the substantia nigra pars compacta. The mechanism by which these DA neurons die is still unclear and under investigation. Although mostly idiopathic, about 10% of PD cases have shown familial inheritance. Mutations in leucine-rich repeat kinase 2 (LRRK2), a large multi-domain protein with unknown physiological and pathological roles, have been linked to PD cases of autosomal dominant inheritance. A PD Drosophilamelanogaster model over expressing the human LRRK2(I2020T) kinase mutant using the GAL4/UAS system has shown a loss of DA neurons and locomotor deficiency. Additionally, ectopic overexpression of human LRRK2 in the eye caused a damaged eye phenotype characterized by roughness of the surface, loss of pigmentation and presence of black lesions (Venderova Ket. al., 2009). The presence of this identifiable eye phenotype has allowed us to perform a suppressor/enhancer screen to identify possible genetic interactors of LRRK2. The LRRK2(I2020T) transgenic flies were crossed with genomic deficiency lines and the eye phenotype screened for either suppression or enhancement. Twenty-two genes, which are implicated in a variety of biological processes, have been identified thus far. Fourteen of these 22 interacting genes were assessed in the DA neurons of the D.melanogaster model. This functional screen is a rapid method to provide us with potential genetic interactions between LRRK2 and other genes, which will in turn, aid in elucidating the functional role of LRRK2 in PD pathology.