Aplicações repetitivas do herbicida glyphosate podem resultar em mudanças nas populações de plantas daninhas, devido a uma pressão seleção sobre espécies de plantas daninhas tolerantes ao herbicida. No entanto, os mecanismos de tolerância dessas espécies não estão completamente compreendidos, e a elucidação desses mecanismos é fundamental nas recomendações de estratégias para o manejo de plantas daninhas tolerantes ao glyphosate. Sendo assim, os objetivos desta pesquisa foram: (i) avaliar a dinâmica do banco de sementes das espécies de plantas daninhas Commelina benghalensis L., Ipomoea grandifolia (Dammer) O’Don e Richardia brasiliensis Gomez (tolerantes), Amaranthus hybridus L. e Galinsoga parviflora Cav. (suscetíveis), em áreas tratadas intensivamente com doses de glyphosate por dois anos consecutivos, (ii) caracterizar os mecanismos de tolerância C. benghalensis e I. grandifolia ao herbicida glyphosate e (iii) estudar a eficácia de controle sobre as plantas daninhas tolerantes por herbicidas alternativos isolados e em mistura com glyphosate. Nos estudos de dinâmica do banco de sementes foram coletadas periodicamente amostras de solos de uma área com aplicação repetitiva do glyphosate para analise quantitativa e qualitativa através de extração das sementes e de germinação de sementes em casa-de-vegetação. Na caracterização dos mecanismos de tolerância, experimentos foram instalados para quantificar a absorção e a translocação de 14 C glyphosate pelas plantas C. benghalensis, I. grandifolia, A. hybridus e Glycine max resistente (R) e suscetível (S) ao glyphosate, às 2, 4, 8, 12, 24, 48 e 72 horas após tratamento (HAT); analisando-se, ainda, o metabolismo do glyphosate pelas plantas daninhas a 72 HAT. Cromatografia de camada delgada foi utilizada para determinar à composição química das ceras epicuticulares e microscopia eletrônica de varredura para a caracterização da superfície foliar das plantas daninhas. Experimento em casa de vegetação com os herbicidas alternativos carfentrazone, flumioxazin, sulfentrazone, chlorimuron-ethyl e bentazon foi conduzido para testar as interações aditivas, sinergísticas ou antagônicas com glyphosate no controle de plantas daninhas tolerantes. Os resultados foram: Ao final de dois anos de aplicação repetitiva de glyphosate houve um acréscimo do banco de sementes das plantas daninhas tolerantes, e um decréscimo no banco de sementes das plantas daninhas sensíveis ao glyphosate. A taxa de absorção e translocação do 14 C glyphosate em A. hybridus e Glycine max R e S foi maior que das demais plantas estudadas; I. grandifolia apresentou excelente taxa de absorção, entretanto, a translocação desta espécie foi reduzida. Em C. benghalensis a taxa de absorção foliar do glyphosate foi baixa, porém, não houve impedimento a translocação. Em C. benghalensis foi encontrado, além de glyphosate, o metabólito ácido aminometilfosfônico (AMPA). As ceras da cutícula foliar de A. hybridus e I. grandifolia apresentaram características predominantemente hidrofílicas, e a superfície foliar não apresentou tricomas, sendo observado uma grande quantidade de estômatos. As ceras epicuticulares destas duas espécies de plantas daninhas apresentaram forma cristalina apenas em A. hybridus. Em C. benghalensis as ceras são relativamente mais hidrofóbicas, o que pode ter influenciado na menor penetração de glyphosate; sendo que, a superfície foliar apresenta tricomas e um número menor de estômatos, e estes estão recobertos por cera epicuticular. / Intensive applications of the herbicide glyphosate for weed control may result in the weed population changes, through selection pressure of the glyphosate tolerant weed species. However, the glyphosate tolerance mechanisms of these species are not completely understood, and the elucidation of these mechanisms is fundamental to the recommendations of alternative herbicides to glyphosate tolerant weed management. Therefore, the objectives of this research were: (i) to evaluate the weed seed bank dynamic of the species Commelina benghalensis L., Ipomoea grandifolia (Dammer) O’Don and Richardia brasiliensis Gomez (tolerants to glyphosate), Amaranthus hybridus L. and Galinsoga parviflora Cav. (susceptibles to glyphosate), in areas that had been intensively treated with glyphosate rates for two consecutive years; (ii) to characterize the glyphosate tolerance mechanisms of C. benghalensis and I. grandifolia, and (iii) to study the efficacy of alternative herbicides in mixture with glyphosate in the control of tolerant weeds. The weed seed bank dynamic was evaluated through soil samples collected periodically, and analysed quantitative and qualitativelly by greenhouse germination test and seeds counting. Experiments were conducted to caracterize the absorption and translocation of 14 C glyphosate by C. benghalensis, I. grandifolia, A. hybridus and Glycine max resistant (R) and susceptible (S) to glyphosate, at 2, 4, 8, 12, 24, 48 and 72 hours after treatment (HAT); and analysing the glyphosate metabolism by the weeds at 72 HAT. Thin layer chromatography was used to determine the chemical composition of the epicuticular layers and eletronic microscopy to caracterize the foliar surface of the weeds. Greenhouse experiment with the alternative herbicides carfentrazone, flumioxazin, sulfentrazone, chlorimuron-ethyl and bentazon were conducted to test aditive, sinergistic or antagonic interactions with glyphosate on the control of the tolerant weeds. After two years of repetitive glyphosate application there was an increase in the weed seed bank of the tolerant species, and a decrease of the weed seed bank of the susceptible species to glyphosate. The rate of absorption and translocation of the 14 C glyphosate by A. hybridus and Glycine max R and S was higher than the other studied plants, likewise I. grandifolia presented an excelent rate of absorption, even tough, the translocation of this species was reduced. In C. benghalensis the glyphosate rate of foliar absorption was low, however, there was a free translocation of the herbicide. No glyphosate metabolites were observed in I. grandifolia and A. hybridus, however, in C. benghalensis it was found, besides glyphosate, the aminophosphonic acid (AMPA) metabolite. The cuticle waxes of A. hybridus and I. grandifolia presented predominantly hydrophilic characteristics, and the foliar surface did not present tricomes, being observed a great amount of stomata. The epicuticular waxes of this two weed species presented crystal form only in A. hybridus. In C. benghalensis the waxes were relatively more hidrofobics, which could be influenced on lower penetration of glyphosate; the foliar surface present tricomes and fewer stomata, and these are recovered by epicuticular wax.

O acúmulo significativo de conhecimento sobre a ecologia de restauração nos últimos anos tem conduzido a mudanças na orientação dos programas de manejo e restauração florestal. A escolha ou criação de um modelo de restauração é um processo em constante aprimoramento, exigindo a necessidade de estudos que não apenas diminuam os custos envolvidos, mas também, que garantam a efetividade do objetivo proposto. Neste sentido, este trabalho testa novas metodologias de restauração visando a obtenção de informações técnicas sobre: A) a expressão do banco de sementes autóctone e alóctone na restauração florestal de diferentes situações de degradação antrópica.; B) o resgate de plântulas e indivíduos de espécies arbustivo-arbóreas regenerantes; C) a eficiência de um modelo de plantio que utiliza conceitos de Preenchimento e Diversidade e; D) a evolução da paisagem. Para análise dos bancos de sementes autóctone e alóctone foram feitos levantamentos da densidade e das espécies arbustivo-arbóreas regeneradas. No resgate de indivíduos de espécies arbustivo-arbóreas regenerantes, as plântulas e indivíduos jovens foram resgatadas em uma floresta e levadas para adapatação em viveiro, onde tiveram seus indivíduos e espécies contabilizados em termos de densidade e sobrevivência. No modelo de plantio estudado, as espécies foram reunidas em grupos de Preenchimento e Diversidade e tiveram a cobertura de copa de cada indivíduo quantificada. A evolução da paisagem foi realizada através de fotointerpretação e principalmente checagem de campo periódica. Os principais resultados dessas metodologias foram: A) As situações identificadas na Fazenda Intermontes apresentaram diferentes expressões de regeneração natural em função do número de indivíduos germinados banco de sementes autóctone. O aproveitamento dessa expressão poderá ser realizado através de metodologias diferenciadas de restauração para cada situação estudada: B) No estudo do Banco de sementes alóctone, a densidade dos indivíduos germinados se foi de 409.334 indivíduos/ha, revelando a possibilidade de uso no programa de restauração. Os indivíduos arbóreos apresentaram na área adubada um acréscimo de 137,3 % em sua altura média em relação aos indivíduos da área não adubada. C) No resgate de plântulas e indivíduos de espécies arbustivo-arbóreas regenerantes, a densidade dos indivíduos foi muito próxima para as duas épocas do ano, sendo 38.700 ind./ha e 37.900 ind./ha, mas com uma diferença significante para os indivíduos sobreviventes que apresentaram 16.500 ind./ha e 26.600 ind./ha para os meses de julho e dezembro de 2002, respectivamente. A técnica de resgate utilizada se mostrou viável uma vez que foi possível alcançar até 70,9% de sobrevivência para os indivíduos resgatados; D) No estudo sobre o modelo de plantio foram amostrados 143 espécies, demonstrando que a função do grupo da diversidade vem sendo mantida e aumentando as chances de efetivação dos processos de sucessão. Os resultados mostraram que o grupo de Preenchimento apresentou-se com uma porcentagem de cobertura significativamente maior que a do grupo de Diversidade, sendo 73,6% e 83,3% para 1,5 e 2,5 anos respectivamente. / Significant accumulation of know-how about restoration ecology has been changing forest restoration management programs’ trends during the last years. Choosing or elaborating a restoration model is a process that requires constant improvement and ecological studies that not only reduces the costs involved but also warrants the achievement of the aimed proposal. This study explores new restoration methodologies, searching for technical information about: A) autochthonous and allochthonous seed bank expression on forest restoration at different situations degraded by anthropogenic disturbances; B) seedlings and woody growing individuals rescue; C) efficiency of a planting model based on fulfillment and diversity concept; D) landscape development. The autochthonous and allochthonous seed bank were analyzed through regenerating woody species and density scouting. For the rescue of regenerating woody species, seedling and young individuals were taken from a forest and transferred to a plant nursery for adjustment, where number and species were counted regarding density and survival. In this studied planting model, species were organized and gathered into a fulfillment or diversity group, and each individual had its crown area quantified. Landscape development was analyzed by photointerpretation and specially by periodical field checking. Results from these methodologies showed that: A) Identified degraded situations at Intermontes farm displayed distinguished natural regeneration expression, influenced by the number of germinated seeds of the autochthonous seed bank. Advantage can be taken from these different outcomes by using distinct restoration methodologies for each studied situation. B) For the autochthonous seed bank study, germinated seed density was 409,334 ind./ha, indicating its potential role on restoration programs. Woody individuals at fertilized sites had a medium height enhancement of 137,7% when compared to those at non fertilized sites. C) For seedlings and woody growing individuals rescue, individual density was similar for both seasons (38700ind./ha and 37900ind./ha) but significantly distinct for individual survivor, with values of 16500ind./ha for July and 2600ind./ha for December. Rescue technique turned out to be a viable possibility since it achieved survival of 70,9% for rescued individuals. D) This planting model study registered 143 species, indicating that the diversity group’s role has been maintained and enhances the chance of successional process success. The fulfilling group had a significant higher percentage of crown area than the diversity group, with 73,6% and 83,3% for 1,5 and 2,5 years, respectively.

Submitted by MARIANA CORREA DAIREL (maridairel@gmail.com) on 2018-05-29T13:32:19Z No. of bitstreams: 1 Dissertação_MarianaDairel.pdf: 2176755 bytes, checksum: 15173e4411a9c0edf054f8425b0128b8 (MD5) / Rejected by Adriana Aparecida Puerta null (dripuerta@rc.unesp.br), reason: Prezada Mariana, O documento enviado para a coleção Instituto de Biociências Rio Claro foi recusado pelo(s) seguinte(s) motivo(s): - Falta a folha de aprovação, que deve ser solicitada à Seção de Pós-Graduação e deve ser inserida após a ficha catalográfica. - Falta o Abstract (geral) e as palavras chaves em inglês, que devem vir após o Resumo em português. Em caso de dúvidas entre em contato pelo email repositoriounesp@reitoria.unesp.br. Agradecemos a compreensão e aguardamos o envio do novo arquivo. Atenciosamente, Biblioteca Campus Rio Claro Repositório Institucional UNESP https://repositorio.unesp.br on 2018-05-29T14:15:32Z (GMT) / Submitted by MARIANA CORREA DAIREL (maridairel@gmail.com) on 2018-05-29T14:58:54Z No. of bitstreams: 1 Dissertação_MarianaDairel_2018.pdf: 3026271 bytes, checksum: 1b8e54d60ef03c2c75e6584b82e1ad04 (MD5) / Approved for entry into archive by Adriana Aparecida Puerta null (dripuerta@rc.unesp.br) on 2018-05-29T15:23:22Z (GMT) No. of bitstreams: 1 dairel_mc_me_rcla.pdf: 2894726 bytes, checksum: a451a6d4fa2118d57b4a79457d9f2c49 (MD5) / Made available in DSpace on 2018-05-29T15:23:22Z (GMT). No. of bitstreams: 1 dairel_mc_me_rcla.pdf: 2894726 bytes, checksum: a451a6d4fa2118d57b4a79457d9f2c49 (MD5) Previous issue date: 2018-04-27 / Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) / Espécies invasoras são aquelas capazes de se estabelecer em áreas além de sua ocorrência natural, ultrapassando barreiras geográficas e constituindo novas populações viáveis. A introdução de gramíneas africanas no Cerrado, como Melinis minutiflora P. Beauv e Urochloa brizantha (Stapf) R.D. Webster, pode levar a uma baixa heterogeneidade do sistema, deixando-o mais suscetível a distúrbios ambientais, alterando o equilíbrio da comunidade e os serviços ecossistêmicos. Em sistemas frequentemente perturbados, clareiras são criadas na paisagem e novas espécies são recrutadas para colonizar o espaço aberto, processo esse regido pelo nicho da regeneração. Espera-se que estes nichos se sobressaiam nas espécies invasoras em relação às espécies nativas, conferindo-lhes vantagens adaptativas na colonização desses espaços. Dessa maneira, o objetivo do estudo foi avaliar a dinâmica do banco de sementes de gramíneas em áreas com e sem invasão biológica, além de avaliar diferentes fatores que influenciam a germinação de gramíneas nativas. A área de estudo está inserida na Estação Ecológica de Itirapina, onde até 1998 havia uma plantação de Pinus caribaea, a qual foi removida e desde então a área está sob regeneração natural, sem interferência, com alto grau de invasão por gramíneas africanas. No primeiro capítulo, as amostras de solo foram coletadas nas parcelas experimentais controle do projeto da Profa. Dra. Alessandra Fidelis (FAPESP 2015/06743-0), delineado então parcelas com três tratamentos: dominada por uma das duas espécies invasoras, Melinis minutiflora e Urochloa brizantha, e parcelas com domínio de vegetação nativa (4parcelas/tratamento= 12 unidades amostrais). No segundo capítulo, as sementes de gramíneas nativas foram coletadas na Estação Ecológica de Itirapina e na Reserva Natural Serra do Tombador, triadas em laboratório e submetidas a tratamentos de flutuação de temperatura, simulando a oscilação de temperatura diária sobre a superfície do solo, e choques de temperatura simulando a passagem do fogo. O conhecimento mais detalhado sobre o banco de sementes em áreas invadidas é fundamental para elaboração de práticas de controle e manejo dessas espécies. Além disso, a germinação e viabilidade das sementes de gramíneas nativas são fatores importantes tanto para elucidar a regeneração natural via semente, compreendendo processos ecológicos relacionados à quebra da dormência fisiológica, quanto para aplicação de métodos físicos de restauração do Cerrado, como a semeadura direta. / Invasive species are those capable of establishing themselves in areas beyond its natural occurrence, surpassing geographical barriers and constituting new populations. The introduction of African grasses in Cerrado, Melinis minutiflora P. Beauv and Urochloa brizantha (Stapf) R.D. Webster, may low heterogeneity of the system, making it more susceptible to altering the balance of the community and ecosystems. In frequently disturbed systems, clearings are created in the landscape and new species are recruited to colonize the open space, which is governed by the regeneration niche. These niches are expected to be better in invasive species in relation to native species, giving them adaptive advantages in the colonization of these spaces. In this way, the objective of the study was to evaluate the dynamics of grasslands in areas with and without biological invasion, in addition to evaluating different factors that influence the germination of native grasses. The study area is located in the Estação Ecológica de Itirapina, where until 1998 there was a plantation of Pinus caribaea, which has been removed and since then the area is under natural regeneration, without interference, with a high invasion of Urochloa brizantha and Melinis minutiflora. In the first chapter, the soil samples were collected in the control plots of the project of Profa. Dr. Alessandra Fidelis (FAPESP 2015 / 06743-0), outlined then plots with three treatments: dominated by one of the two invasive species, Urochloa brizantha and Melinis minutiflora, and plots with dominance of native vegetation (4plots / treatment = 12 sample units). In the second chapter, grass seeds were collected at Estação Ecológica de Itirapina and in Reserva Natural Serra do Tombador, triads in the laboratory and submitted to treatments of temperature fluctuation, simulating the daily temperature oscillation on the soil surface, and heat shock simulating the passage of fire. More detailed knowledge about the soil seed bank in invaded areas is fundamental for the elaboration of practices of control and management of these species. In addition, the germination and viability of native grass seeds are important factors both to elucidate the natural regeneration through seed, comprising ecological processes related to break physiological dormancy of seed, and for application of physical methods of restoration of Cerrado, such as direct sowing.

Aplicações repetitivas do herbicida glyphosate podem resultar em mudanças nas populações de plantas daninhas, devido a uma pressão seleção sobre espécies de plantas daninhas tolerantes ao herbicida. No entanto, os mecanismos de tolerância dessas espécies não estão completamente compreendidos, e a elucidação desses mecanismos é fundamental nas recomendações de estratégias para o manejo de plantas daninhas tolerantes ao glyphosate. Sendo assim, os objetivos desta pesquisa foram: (i) avaliar a dinâmica do banco de sementes das espécies de plantas daninhas Commelina benghalensis L., Ipomoea grandifolia (Dammer) O’Don e Richardia brasiliensis Gomez (tolerantes), Amaranthus hybridus L. e Galinsoga parviflora Cav. (suscetíveis), em áreas tratadas intensivamente com doses de glyphosate por dois anos consecutivos, (ii) caracterizar os mecanismos de tolerância C. benghalensis e I. grandifolia ao herbicida glyphosate e (iii) estudar a eficácia de controle sobre as plantas daninhas tolerantes por herbicidas alternativos isolados e em mistura com glyphosate. Nos estudos de dinâmica do banco de sementes foram coletadas periodicamente amostras de solos de uma área com aplicação repetitiva do glyphosate para analise quantitativa e qualitativa através de extração das sementes e de germinação de sementes em casa-de-vegetação. Na caracterização dos mecanismos de tolerância, experimentos foram instalados para quantificar a absorção e a translocação de 14 C glyphosate pelas plantas C. benghalensis, I. grandifolia, A. hybridus e Glycine max resistente (R) e suscetível (S) ao glyphosate, às 2, 4, 8, 12, 24, 48 e 72 horas após tratamento (HAT); analisando-se, ainda, o metabolismo do glyphosate pelas plantas daninhas a 72 HAT. Cromatografia de camada delgada foi utilizada para determinar à composição química das ceras epicuticulares e microscopia eletrônica de varredura para a caracterização da superfície foliar das plantas daninhas. Experimento em casa de vegetação com os herbicidas alternativos carfentrazone, flumioxazin, sulfentrazone, chlorimuron-ethyl e bentazon foi conduzido para testar as interações aditivas, sinergísticas ou antagônicas com glyphosate no controle de plantas daninhas tolerantes. Os resultados foram: Ao final de dois anos de aplicação repetitiva de glyphosate houve um acréscimo do banco de sementes das plantas daninhas tolerantes, e um decréscimo no banco de sementes das plantas daninhas sensíveis ao glyphosate. A taxa de absorção e translocação do 14 C glyphosate em A. hybridus e Glycine max R e S foi maior que das demais plantas estudadas; I. grandifolia apresentou excelente taxa de absorção, entretanto, a translocação desta espécie foi reduzida. Em C. benghalensis a taxa de absorção foliar do glyphosate foi baixa, porém, não houve impedimento a translocação. Em C. benghalensis foi encontrado, além de glyphosate, o metabólito ácido aminometilfosfônico (AMPA). As ceras da cutícula foliar de A. hybridus e I. grandifolia apresentaram características predominantemente hidrofílicas, e a superfície foliar não apresentou tricomas, sendo observado uma grande quantidade de estômatos. As ceras epicuticulares destas duas espécies de plantas daninhas apresentaram forma cristalina apenas em A. hybridus. Em C. benghalensis as ceras são relativamente mais hidrofóbicas, o que pode ter influenciado na menor penetração de glyphosate; sendo que, a superfície foliar apresenta tricomas e um número menor de estômatos, e estes estão recobertos por cera epicuticular. / Intensive applications of the herbicide glyphosate for weed control may result in the weed population changes, through selection pressure of the glyphosate tolerant weed species. However, the glyphosate tolerance mechanisms of these species are not completely understood, and the elucidation of these mechanisms is fundamental to the recommendations of alternative herbicides to glyphosate tolerant weed management. Therefore, the objectives of this research were: (i) to evaluate the weed seed bank dynamic of the species Commelina benghalensis L., Ipomoea grandifolia (Dammer) O’Don and Richardia brasiliensis Gomez (tolerants to glyphosate), Amaranthus hybridus L. and Galinsoga parviflora Cav. (susceptibles to glyphosate), in areas that had been intensively treated with glyphosate rates for two consecutive years; (ii) to characterize the glyphosate tolerance mechanisms of C. benghalensis and I. grandifolia, and (iii) to study the efficacy of alternative herbicides in mixture with glyphosate in the control of tolerant weeds. The weed seed bank dynamic was evaluated through soil samples collected periodically, and analysed quantitative and qualitativelly by greenhouse germination test and seeds counting. Experiments were conducted to caracterize the absorption and translocation of 14 C glyphosate by C. benghalensis, I. grandifolia, A. hybridus and Glycine max resistant (R) and susceptible (S) to glyphosate, at 2, 4, 8, 12, 24, 48 and 72 hours after treatment (HAT); and analysing the glyphosate metabolism by the weeds at 72 HAT. Thin layer chromatography was used to determine the chemical composition of the epicuticular layers and eletronic microscopy to caracterize the foliar surface of the weeds. Greenhouse experiment with the alternative herbicides carfentrazone, flumioxazin, sulfentrazone, chlorimuron-ethyl and bentazon were conducted to test aditive, sinergistic or antagonic interactions with glyphosate on the control of the tolerant weeds. After two years of repetitive glyphosate application there was an increase in the weed seed bank of the tolerant species, and a decrease of the weed seed bank of the susceptible species to glyphosate. The rate of absorption and translocation of the 14 C glyphosate by A. hybridus and Glycine max R and S was higher than the other studied plants, likewise I. grandifolia presented an excelent rate of absorption, even tough, the translocation of this species was reduced. In C. benghalensis the glyphosate rate of foliar absorption was low, however, there was a free translocation of the herbicide. No glyphosate metabolites were observed in I. grandifolia and A. hybridus, however, in C. benghalensis it was found, besides glyphosate, the aminophosphonic acid (AMPA) metabolite. The cuticle waxes of A. hybridus and I. grandifolia presented predominantly hydrophilic characteristics, and the foliar surface did not present tricomes, being observed a great amount of stomata. The epicuticular waxes of this two weed species presented crystal form only in A. hybridus. In C. benghalensis the waxes were relatively more hidrofobics, which could be influenced on lower penetration of glyphosate; the foliar surface present tricomes and fewer stomata, and these are recovered by epicuticular wax.

Soil seed banks are a reservoir of viable seeds present in the soil in plant communities. They have been studied and characterized in various ways in different habitats. However, these studies are limited to modern seed banks. This study extends seed bank studies to the Paleozoic Era. It was hypothesized that size distribution and seed density in Paleozoic seed banks exhibit similar patterns as in modern seed banks. Seed sizes and seed density of fossil seed from Wise Virginia were estimated. Modern seed bank information was obtained from published data. Data were analyzed using one-way ANOVA and Kruskal-Wallis test. The Paleozoic size distribution was predominated by larger seeds and the estimated seed density of 19 200 seeds m-3 falls within the range of modern seed banks but at a higher end of modern seed bank densities. During the Paleozoic they were sufficient to insure regeneration of these economically important forests.

Increased fire frequency, livestock grazing and other disturbances have caused type-conversion of native chaparral to non-native annual grasslands. Competition with non-native annual grasses hinders shrub establishment, presenting challenges in restoration. This study examined the feasibility of chaparral restoration in a Mediterranean-type climate, on a type-converted landscape in San Timoteo Canyon, Riverside County, California. The objectives of this study were to (1) compare the effectiveness of the broad-spectrum herbicide, glyphosate, to that of the grass specific herbicide, fluazifop, at eliminating non-native annual grasses; (2) compare the success of seeding or planting native seedlings as a means of restoration; (3) analyze the soil seed bank to determine if there was a relict native species seed bank on site; (4) test the field application of smoke-water as a technique to manipulate the soil’s native shrub seed-bank for restoration. A factorial design was utilized, with three replicates: four restoration treatments (no restoration, smoke-water application, seeding, and planting seedlings) across three herbicide treatments (a January glyphosate application followed by an early-March fluazifop treatment, an early-March fluazifop treatment only, and a control treatment with no herbicide). Glyphosate application followed by fluazifop application proved to be more effective at reducing non-native annual grasses and benefitted shrub establishment more effectively than the fluazifop and control treatments. The application of glyphosate in winter promoted shrub establishment and growth, and increased soil moisture, while preventing the springtime increase in grass cover that was seen in the control and fluazifop-only treatments. The four chaparral species that were planted (Adenostoma fasciculatum, Eriogonum fasciculatum, Quercus berberidifolia, Rhus ovata) did not behave similarly. No Quercus berberidifolia individuals survived. Eriogonum fasciculatum did not exhibit differences in survival in any herbicide treatments, but exhibited higher live plant canopy volume in plots that received glyphosate with fluazifop follow-up treatments. Adenostoma fasciculatum and Rhus ovata showed higher survival in plots that received glyphosate with fluazifop follow-up treatments; however, neither exhibited differences in live plant canopy volume between herbicide treatments. These results persisted until the end of the study, 14 months after planting. Seed application of seven species (Adenostoma fasciculatum, Artemisia californica, Eriogonum fasciculatum, Gutierrezia sarothrae, Quercus berberidifolia, Rhus aromatica, and Rhus ovata) was not successful at this study site. Seed bank analysis revealed that there was a high density of non-native seeds present, and that the relict native shrub seed bank was insufficient for restoration on this site. Smoke-water application at the study site proved to be ineffective at manipulating the soil seed bank, as no chaparral species emerged. Results from this study suggest that winter glyphosate application followed by a fluazifop application in a Mediterranean-type climate may benefit shrub re-establishment more than spring fluazifop-only treatments. Results indicate that winter herbicide treatment will eliminate competitive non-native annual grasses early in the growing season, resulting in increased soil moisture availability for chaparral shrubs to utilize. Winter glyphosate application, supplemented with the planting of seedlings, could be a successful technique to re-establish chaparral shrubs on type-converted slopes.

The conversion of sagebrush-steppe communities of the Great Basin into cheatgrass-dominated communities is one of the most dramatic ongoing land conversions in North America. Although restoration of these communities is a high priority to landowners and land management agencies, restoration of native vegetation is difficult. Several restoration treatments intended to increase the success of aerially‐seeded perennial grasses in cheatgrass-invaded sagebrush ecosystems were assessed to determine their effects on perennial seedling emergence and soil seed bank density and composition. Assessed restoration treatments were: 1) vegetation manipulation (sagebrush thinning and prescribed burning); 2) imazapic herbicide application; 3) seedbed amendments (aerial seeding with activated carbon addition, aerial seeding with sucrose addition); and 4) seeding frequency. The effects of these treatments were evaluated in two distinct sagebrush shrubland ecosystems in northern Utah. One is characterized as a remnant sagebrush stand with a cheatgrass-dominated understory and the other as a cheatgrass near-monoculture, completely lacking a sagebrush component. In the seed bank study, responses were assessed immediately and 1 year following treatment while in the seedling emergence study, they were assessed 2 and 3 years following treatment. Main effects of vegetation manipulation, herbicide application, and seedbed addition treatments and their interactions on perennial seedling emergence are described in Chapter 2. The effects of seeding frequency on perennial seedling emergence are also described in Chapter 2. Herbicide demonstrated potential for increasing native perennial grass emergence, although this response was delayed and not seen until 3 years post-application. Burning showed potential for increasing the emergence of perennial grasses 2 years post-burn. Results also suggest that potential exists to increase native perennial grass emergence through an increase in seeding frequency. In Chapter 3 I evaluated the effects of vegetation manipulation, herbicide application, and seedbed addition on seed pool dynamics. These results suggest that herbicide and sucrose may be useful tools for reducing exotic species richness in cheatgrass-invaded systems. Herbicide also showed potential for reducing cheatgrass seed bank densities. Additionally, results demonstrated that the reductions in cheatgrass seed bank densities observed immediately after fire are still observed 1 year post-burn.

The conversion of sagebrush-steppe communities of the Great Basin into cheatgrass-dominated communities is one of the most dramatic ongoing land conversions in North America. Although restoration of these communities is a high priority to landowners and land management agencies, restoration of native vegetation is difficult. Several restoration treatments intended to increase the success of aerially‐seeded perennial grasses in cheatgrass-invaded sagebrush ecosystems were assessed to determine their effects on perennial seedling emergence and soil seed bank density and composition. Assessed restoration treatments were: 1) vegetation manipulation (sagebrush thinning and prescribed burning); 2) imazapic herbicide application; 3) seedbed amendments (aerial seeding with activated carbon addition, aerial seeding with sucrose addition); and 4) seeding frequency. The effects of these treatments were evaluated in two distinct sagebrush shrubland ecosystems in northern Utah. One is characterized as a remnant sagebrush stand with a cheatgrass-dominated understory and the other as a cheatgrass near-monoculture, completely lacking a sagebrush component. In the seed bank study, responses were assessed immediately and 1 year following treatment while in the seedling emergence study, they were assessed 2 and 3 years following treatment. Main effects of vegetation manipulation, herbicide application, and seedbed addition treatments and their interactions on perennial seedling emergence are described in Chapter 2. The effects of seeding frequency on perennial seedling emergence are also described in Chapter 2. Herbicide demonstrated potential for increasing native perennial grass emergence, although this response was delayed and not seen until 3 years post-application. Burning showed potential for increasing the emergence of perennial grasses 2 years post-burn. Results also suggest that potential exists to increase native perennial grass emergence through an increase in seeding frequency. In Chapter 3 I evaluated the effects of vegetation manipulation, herbicide application, and seedbed addition on seed pool dynamics. These results suggest that herbicide and sucrose may be useful tools for reducing exotic species richness in cheatgrass-invaded systems. Herbicide also showed potential for reducing cheatgrass seed bank densities. Additionally, results demonstrated that the reductions in cheatgrass seed bank densities observed immediately after fire are still observed 1 year post-burn.

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Integrating cultural weed management practices with herbicides is an important strategy to reduce wild oat (Avena fatua L.) populations in Alberta, Canada. The purpose of this thesis is to expand the knowledge on wild oat seed banks and seedling emergence within integrated weed management systems. Field experiments were conducted from 2006-2007 to examine the impact of crop rotation, barley cultivar, barley seeding rate and herbicide rate on wild oat seed bank density, seed mortality, seedling emergence and seedling survivorship. Management systems consisting of diverse crop rotations, tall barley cultivars and increased barley seeding rates reduced wild oat seed bank density, seedling emergence and seedling survivorship, especially at low herbicide rates. Wild oat seed banks predicted subsequent wild oat emergence, whereas biomass and emergence strongly predicted seed banks. Lastly, 50% wild oat emergence was achieved at 537 and 509 growing degree days in 2006 and 2007, respectively, in Lacombe, Alberta. / Plant Science