Inorganic based membranes which have a symmetric/asymmetric structure have been produced using an immersion induced phase inversion and sintering method. An organic binder solution (dope) containing yttria-stabilised zirconium (YSZ) particles is spun through a triple orifice spinneret to form a hollow fibre precursor, which is then sintered at elevated temperatures to form a ceramic support. The phase inversion process for the formation of hollow fibre membranes was studied in order to produce the best morphological structure/support for palladium based membranes. The spinning parameters, particle size, non-solvent concentration, internal coagulant as well as the calcination temperature were investigated in order to determine the optimum values. Sintering temperature was also investigated, which would yield a sponge-like structure with an optimized permeability, while retaining a smooth outer surface. The supports produced by phase inversion were characterized in terms of dimension by mercury porosimetry, compressed air permeability, Surface Electron Microscopy (SEM) and Atomic Force Microscopy (AFM). The morphology of the produced ceramic support showed either dense or porous characteristics governed by the dynamics of the phase inversion process. The particle size of YSZ was examined in order to decrease the amount of agglomerates in the spinning suspension. Zetasizer tests indicated that at 15 minutes, the ultrasonic bath effectively homogenised the YSZ particles and prohibited soft agglomerates from reforming in the spinning suspension. In this study, an increase in air gap had no noticeable effect on the finger like voids but it had a considerable effect on both the inner diameter (ID) and outer diameter (OD) of the green fibres, while an increase in bore liquid flow rate and extrusion pressure promoted viscous fingering and significant effect on the ID and OD of the fibres, respectively. There was a decrease in porosity and permeability with increasing sintering temperature, addition of water concentration in the spinning suspension and varying Nmethylpyrrolidone (NMP) aqueous solution of the internal coagulant. The amount of YSZ added to the starting suspension influenced the properties of the support structure. Viscous deformation was observed for dope with lower particle loading thus resulted in the formation of cracks and defects during sintering. / >Magister Scientiae - MSc

An introduction to nematode systematics is provided which deals broadly with the history of the classification of nematodes, the controversial usage of the Phylum names Nemata Cobb, 1919 and Nematoda (Rudolphi, 1808) Lankester, 1877 and the reason why the name Nematoda was used in the present study. The classification, diagnosis and bionomics of the genus Aphelenchoides Fischer, 1894, the genus to which A. arachidis Bos, 1977 belongs is discussed. The section on bionomics is included to capture the astounding ability of this group of organisms to adapt to different trophic levels, a concept that is used to attempt an explanation for the ability of a supposedly African nematode, A. arachidis, to infest an alien crop species (groundnut). The ability of Aphelenchoides spp. to adapt to different host plant species is discussed, as well as the ability of the groundnut plant to mature its pods underground, a characteristic that predisposes these plants to a host of pathogens. The damage caused by two of the most important endoparasitic nematode species on groundnut, A. arachidis and Ditylenchus africanus Wendt, Swart, Vrain & Webster, 1995 were compared with each other. The South African population of A. arachidis was found predominantly in the shells of groundnut, whereas they were found in the shells, roots, hypocotyls and testas of the groundnut plants in Nigeria. The present study showed that A. arachidis and D. africanus occur together in groundnut in South Africa with D. africanus usually being the dominant species. In only one instance, at Bullhill (Vaalharts Irrigation Scheme, Northern Cape), the groundnut shells and testas were infested by A. arachidis alone. The importance of plant quarantine in South Africa is dealt with and the aims and principles of quarantine, as well as the different guidelines that have to be adhered to when deciding on the quarantine status of an organism are explained. Descriptions are provided of the methods used to prepare specimens for viewing with the light microscope (LM) and the scanning electron microscope (SEM), as well as the procedures of the molecular study. A morphological and morphometrical description of A. arachidis specimens from South Africa, as well as a comparison with specimens from Nigeria was done. Differences between the South African and Nigerian populations included, respectively, a lower b-value (7 - 11 vs 10 - 18), more lateral lines (2 - 4 vs 2), a slightly shorter stylet (8-10 m vs 10 - 12 m) and a longer length of the post-uterine sac as a percentage of the distance from vulva to anus (41 - 96 % vs ± 50 %). Scanning electron micrographs of this species are presented for the first time and shows the morphology of the lip region and lateral lines. Since both A. arachidis and A. blastophthorus were detected in the pods, a study was done to evaluate a PCR-based diagnostic method for the identification of these species and to compare the results with those reported in literature. Restriction fragment length polymorphisms (RFLPs) in the rDNA fragment were used to compare and differentiate between nematode species. The differences encountered within the South African population (morphological, morphometrical and molecular) warrant a study of more specimens from more localities. Through this it could be ascertained whether the South African population is a subspecies of A. arachidis or if this species just differs widely between localities. Future research should focus on a survey of the groundnut producing areas in South Africa to determine the distribution and economic impact of A. arachidis. The incidence of A. arachidis on other agricultural crops, especially those used in rotation with groundnut, also needs to be determined. The next issue to address is what enables a supposedly endemic species to Africa, A. arachidis, to parasitize an alien plant species (groundnut) from South America. Screening of the endemic bean family (Fabaceae) in Africa for the presence of A. arachidis, could hold the answer to this question. / Thesis (M. Environmental Science)--North-West University, Potchefstroom Campus, 2008.

Copper-alumina composites were developed for testing in abrasive wear applications. The composites featured a porous continuous ceramic-preform network infiltrated by a liquid metal to form the final consolidated composite. The liquid metal phase was pure copper. Six different ceramic preform variants were tested. Ceramic volume fractions of 40, 50 and 60% were used, of two preform types; one pure-alumina, and one with additional 2wt% copper(I) oxide (CU20), functioning as an infiltration aid, the effects of which were determined in a previous study; the copper-oxide reduced infiltration pressure and allowed the use of higher ceramic phase volume fraction in the final composite. Abrasive wear tests against two automotive braking system materials were conducted. Grey cast iron of alloy type GG15 was used to establish a baseline for behaviour of the six different composite samples and compare them. Following this, the three volume fraction variants of samples using the copper-oxide infiltration aid were trialled against a commercially-available European passenger vehicle brake pad friction material; ABEX 6091. Wear tests were conducted on a pin-on-disc tribometer. Hemispherical-headed pins were made from the composite and tested against rotating discs of the grey cast iron and the ABEX friction material. Contact velocity was kept constant at Ims-?? at room temperature in air, and contact loads up to 15N were used. Test loads of 1-4N were used against grey cast iron, and 15N against the ABEX friction material. Optical micrography was used to monitor the wear rate of samples tested against grey cast iron. Scanning electron microscopy (SEM) was used to characterise bulk microstructures and evaluate surface wear features. Transmission electron microscopy (TEM) was used for further microstructural investigation of the sintering and interfacial features of the undamaged pin samples, as well as damage zones and tribofilm compositions. Focussed ion beam (FIB) milling was used to create subsurface cross-sections of wear regions and prepare TEM samples. The wear performance of the different sample types was compared by ceramic content and preform additives. It was found that the wear resistance of pure-alumina preform composites was dependent on ceramic volume fraction. Increasing ceramic content lead to increased wear resistance. The lower sinter temperature of the samples with the copper oxide additive led to reduced wear resistance compared with the monolithic alumina preforms and changes in ceramic volume fractions were not discernable in wear resistance against grey cast iron. This could be further supported by qualitative micrographic observations. All tests against grey cast iron were dominated by tribochemical film formation, which was determined to be oxidation of the iron which formed at the composite pin contact surface. Further testing of the copper-oxide containing samples against the ABEX friction material revealed a mixed result; the 50 and 60% ceramic volume samples produced near-identical wear performance, while the 40% sample suffered poor wear resistance. The dominant wear mechanism of composite pins tested against the ABEX friction material was abrasive wear. Sub-surface analysis of wear pins revealed a prominent damage layer forming at the contact surface of all pin samples which progressively grew into the bulk material. This layer was believed to have an important effect on the wear behaviour of the materials.

In today’s technological environment, organizational capabilities for managing change are regarded as important for business survival and growth. In particular, dynamic organizational capabilities have attracted considerable research interest over the past decade. Recently several studies have suggested that dynamic capabilities may be associated with a concept termed organizational fitness. What is not clear in this emerging research stream is whether firms with superior organizational fitness are more likely to prosper than unfit firms. In addition, relatively little attention has been directed toward creating a systemic model of dynamic capabilities that explains organizational fitness. The nature of fitness has been intensively debated in the biological sciences over a period of several decades. A confusing variety of fitness definitions have emerged from this literature. The lack of an agreed definition of fitness has resulted in several streams of research on organizational fitness. As a result of this fragmentation, there has been little progress toward answering the question of how to measure organizational fitness. The fragmentation in organizational fitness literature is problematic, because research into the relationship between organizational fitness and firm performance is not well-advanced. In this study, organizational fitness is defined in terms of organizational capability to produce variation. By defining fitness in this way, the tautological criticisms leveled against existing concepts of fitness are avoided. The definition of fitness proposed here accommodates both an evolutionary learning perspective and a perspective of strategic management, and thus reflects an integrative approach to the concept. A notable feature of the literature exploring organizational fitness is that it has been focused on large corporations. However, a growing body of literature suggests that SMEs are different from large firms and need to be examined in their own right. SMEs are important contributors to business in most countries throughout the world. This study addresses that perceived gap in the literature and asks: What relationship, if any, is there between organizational fitness and business performance for SMEs? Theory is developed and tested here by means of a large sample of SMEs in New Zealand. Two distinct aspects of organizational fitness are identified for SMEs. First, survival fitness is associated with generic combinative capabilities. Second, growth fitness is associated with knowledge assimilation and transformation. SME growth fitness and survival fitness are each found to be positively related to business performance under a variety of contexts. Further, an increase of growth fitness and survival fitness provides a means of alleviating selection pressures for SMEs. That is, dynamic capabilities of knowledge assimilation and integration are found to be positively associated with SME business performance. In contrast to studies that advocate SME development of context-dependent capabilities, the findings of this study suggest an alternative perspective: variable selection pressures can be influenced by SMEs with a high level of survival and growth fitness.

Copper-alumina composites were developed for testing in abrasive wear applications. The composites featured a porous continuous ceramic-preform network infiltrated by a liquid metal to form the final consolidated composite. The liquid metal phase was pure copper. Six different ceramic preform variants were tested. Ceramic volume fractions of 40, 50 and 60% were used, of two preform types; one pure-alumina, and one with additional 2wt% copper(I) oxide (CU20), functioning as an infiltration aid, the effects of which were determined in a previous study; the copper-oxide reduced infiltration pressure and allowed the use of higher ceramic phase volume fraction in the final composite. Abrasive wear tests against two automotive braking system materials were conducted. Grey cast iron of alloy type GG15 was used to establish a baseline for behaviour of the six different composite samples and compare them. Following this, the three volume fraction variants of samples using the copper-oxide infiltration aid were trialled against a commercially-available European passenger vehicle brake pad friction material; ABEX 6091. Wear tests were conducted on a pin-on-disc tribometer. Hemispherical-headed pins were made from the composite and tested against rotating discs of the grey cast iron and the ABEX friction material. Contact velocity was kept constant at Ims-?? at room temperature in air, and contact loads up to 15N were used. Test loads of 1-4N were used against grey cast iron, and 15N against the ABEX friction material. Optical micrography was used to monitor the wear rate of samples tested against grey cast iron. Scanning electron microscopy (SEM) was used to characterise bulk microstructures and evaluate surface wear features. Transmission electron microscopy (TEM) was used for further microstructural investigation of the sintering and interfacial features of the undamaged pin samples, as well as damage zones and tribofilm compositions. Focussed ion beam (FIB) milling was used to create subsurface cross-sections of wear regions and prepare TEM samples. The wear performance of the different sample types was compared by ceramic content and preform additives. It was found that the wear resistance of pure-alumina preform composites was dependent on ceramic volume fraction. Increasing ceramic content lead to increased wear resistance. The lower sinter temperature of the samples with the copper oxide additive led to reduced wear resistance compared with the monolithic alumina preforms and changes in ceramic volume fractions were not discernable in wear resistance against grey cast iron. This could be further supported by qualitative micrographic observations. All tests against grey cast iron were dominated by tribochemical film formation, which was determined to be oxidation of the iron which formed at the composite pin contact surface. Further testing of the copper-oxide containing samples against the ABEX friction material revealed a mixed result; the 50 and 60% ceramic volume samples produced near-identical wear performance, while the 40% sample suffered poor wear resistance. The dominant wear mechanism of composite pins tested against the ABEX friction material was abrasive wear. Sub-surface analysis of wear pins revealed a prominent damage layer forming at the contact surface of all pin samples which progressively grew into the bulk material. This layer was believed to have an important effect on the wear behaviour of the materials.

Politicians and social marketers considering whether, and how, to implement a national smoking ban in their countries require sound evidence regarding what the causes of support are amongst key stakeholders, how this support will develop over the short to medium term in which they seek to be re-elected, and how support relates to critical outcomes like enforcement. In response to this need, I use structural equation models to develop a model of the antecedents of support, based on theories of self interest and common sense justice, amongst hospitality industry managers. I show that support is determined more by fairness related constructs than self interest constructs, that support for national smoking bans increases consistently over time, and that the initial level of support, and the rate at which support increases, is positively related to subsequent enforcement behaviour by bar managers, in the year after implementation of such a ban, in New Zealand. I use growth mixture modeling to identify two subgroups of bar managers whose support changes at different rates. First, a class of bar managers with a high proportion of smokers who reported fewer instances of respiratory related health problems, showed low initial support, and whose support for the legislation slowly decreased. And second, a class of bar managers comprised of fewer smokers, but reporting more instances of respiratory related health problems. This class began with a high degree support, and steadily increased in support for the national smoking ban. I discuss the implications of these findings for social marketers, health educationalists, and politicians interested in introducing a similar ban in other countries.

Electrochemical behavior of electrolytic manganese dioxide (EMD), chemically prepared battery grade manganese dioxide (BGM), titanium dioxide (TiO2), lithium iron phosphate (LiFePO4) and lithium manganese phosphate (LiMnPO4) in aqueous lithium hydroxide electrolyte has been investigated. These materials are commonly used as cathodes in non-aqueous electrolyte lithium batteries. The main aim of the work was to determine how the electroreduction/oxidation behavior of these materials in aqueous LiOH compares with that reported in the literature in non-aqueous electrolytes in connection with lithium batteries. An objective was to establish whether these materials could also be used to develop other battery systems using aqueous LiOH as electrolyte. The electrochemical characteristics of the above materials were investigated by subjecting them to slow scan cyclic voltammetry and determining the charge/discharge characteristics of Zn/cathode material-aqueous LiOH batteries. The products of electroreduction/oxidation were characterized by physical techniques using X-ray diffraction (XRD), scanning electron micrography (SEM), X-ray photoelectron spectroscopy (XPS), secondary ion mass spectrometry (SIMS), Thermogravimetric analysis (TG) and infra-red spectrometry (IR). The reduction of ã-MnO2 (EMD) in aqueous lithium hydroxide electrolyte is found to result in intercalation of Li+ into the host structure of ã-MnO2. The process was found to be reversible for many cycles. This is similar to what is known to occur for ã-MnO2 in non-aqueous electrolytes. The mechanism, however, differs from that for reduction/oxidation of ã-MnO2 in aqueous potassium hydroxide electrolyte. KOH electrolyte is used in the state-of-art aqueous alkaline Zn/MnO2 batteries. Alkaline batteries based on aqueous KOH as the electrolyte rely upon a mechanism other than K+ intercalation into MnO2. This mechanism is not reversible. This is explained in terms of the relative ionic sizes of Li+ and K+. The lithium-intercalated MnO2 lattice is stable because Li+ and Mn4+ are of approximately the same size and hence Li+ is accommodated nicely into the host lattice of MnO2. The K+ ion which has almost double the size of Li+ cannot be appropriately accommodated into the host structure and hence the K+ -intercalated MnO2 phase is not stable. Chemically prepared battery grade MnO2 (BGM) is found to undergo electroreduction/oxidation in aqueous LiOH via the same Li+ intercalation mechanism as for the EMD. While the Zn/BGM- aqueous LiOH cell discharges at a voltage higher than that for the Zn/EMD- aqueous LiOH cell under similar conditions, the rechargeability and the material utilization of the BGM cell is poorer. The cathodic behavior of TiO2 (anatase phase) in the presence of aqueous LiOH is not reversible. In addition to LiTiO2, Ti2O3 is also formed. The discharge voltage of the Zn/TiO2- aqueous LiOH cell and material utilization of the TiO2 as cathode are very low. Hence TiO2 is not suitable for use in any aqueous LiOH electrolyte battery. LiFePO4 (olivine-type structure) as a cathode undergoes electrooxidation in aqueous LiOH forming FePO4. However the subsequent reduction forms not only the original LiFePO4 but also Fe3O4. Thus the process is not completely reversible and hence LiFePO4 is not a suitable material for use as a cathode in aqueous battery systems. LiMnPO4 (olivine-type structure) undergoes reversible electrooxidation in aqueous LiOH forming MnPO4. The charge/discharge voltage profile of the Zn/MnPO4-aqueous LiOH cell, its coulombic efficiency and rechargeability are comparable to that of the cell using ã-MnO2. EMD and LiMnPO4 both have the potential for use in rechargeable batteries using aqueous LiOH as the electrolyte. Recommendations for further developmental work for such batteries are made.

Doutor -- Universidade de Sao Paulo. Faculdade de Medicina, Sao Paulo, 2002.

Το πρόγραμμα συντήρησης του μοναστηριακού συγκροτήματος του Αγίου Λωτ περιελάμβανε in situ επεμβάσεις συντήρησης, σωστική αποκόλληση και επανατοποθέτηση ενός από τα έξι ψηφιδωτά δάπεδα που βρίσκονταν στην Βασιλική και την ανασκαφή ενός ψηφιδωτού που είχε καταρρεύσει και κατακερματιστεί σε εκατοντάδες θραύσματα στην κλιτύ του λόφου, όπου βρίσκεται η Μονή. Η αποκόλληση του πρώτου ψηφιδωτού δαπέδου και η επακόλουθη ανακάλυψη ενός πρωιμότερου ψηφιδωτού δαπέδου κάτω από αυτό, καθώς και η εύρεση ενός ακόμα σε θραύσματα μας έδωσε την ευκαιρία να παρατηρήσουμε και να καταγράψουμε επιμελώς τις τεχνικές που χρησιμοποιήθηκαν στην κατασκευή των ψηφιδωτών. Ο χαρακτηρισμός επιλεγμένων δειγμάτων κονιαμάτων μας επέτρεψε να προσδιορίσουμε τη σύστασή τους και να ταυτοποιήσουμε το είδος των αδρανών και των προσμίξεων που εμπεριέχει. Οι τεχνικές που χρησιμοποιήθηκαν για την ανάλυση των κονιαμάτων είναι οι: η Περιθλασιμετρία κόνεως ακτίνων-Χ (XRD), η Ηλεκτρονική Μικροσκοπία Σάρωσης (SEM- EDS), η Φασματοσκοπία Απορρόφησης Υπερύθρου (FTIR) και η Οπτική Μικροσκοπία (ΟΜ). Επιπλέον, η πετρογραφική ανάλυση των ψηφίδων, που έγιναν από συνεργάτες του έργου, μας έδωσαν πληροφορίες σχετικά με την προέλευση των πρώτων υλών που χρησιμοποιήθηκαν. Μετά από αναλύσεις και λεπτομερή παρατήρηση, λάβαμε πληροφορίες σχετικά με τις πρώτες ύλες, την προετοιμασία των κονιαμάτων, την στρωματογραφία του ψηφιδωτού δαπέδου, την μέθοδο εκτέλεσης των εργασιών κατασκευής, αλλά και τον τρόπο επεξεργασίας της επιφάνειας. Οι διαφορές που παρατηρούνται στην στρωματογραφία και τη σύσταση των κονιαμάτων, όπως και στις ψηφίδες σε ψηφιδωτά που δημιουργήθηκαν σε διαφορετική χρονική περίοδο συμβάλλουν στην αρχαιολογική μελέτη και κυρίως στην κατανόηση της αρχιτεκτονικής ενότητας και μετατροπών που υπέστη κατά την διάρκεια της χρήσης του χώρου. / The conservation project at the monastic complex of Saint Lot involved the rescue lifting of one of the six mosaics which paved the basilica as well as the excavation of a second one that was found in hundreds of fragments fallen on the slope. The lifting of the first mosaic and the consequent discovery of fragments of an earlier mosaic floor underneath it, as well as the fragmentary state of the second mosaic gave us the opportunity to observe and record thoroughly the techniques employed in the construction of these mosaics. Characterization of selected mortar samples allowed the determination of their composition, identification of the aggregate and its additives. X-Ray Diffraction, Fourier transform infrared spectroscopy, Optical Microscopy and Scanning Electron Microscopy with EDS were the main techniques employed. Mineralogical analyses of tesserae samples, that was employed from other partners of the project, provided information about the provenance of the raw materials that were used. Through detailed observation and analysis, significant information on the raw material selection, preparation of mortars, bedding stratigraphy, method of execution, as well as aspects of tesserae cutting and surface finishing techniques were documented. The variation in the bedding stratigraphy, the composition of the mortars as well as the petrography of the tesserae between mosaics that were executed in different periods contribute in the study of the archaeological context and especially in understanding the succession of the renovation and modification phases of the building.

Το αντικείμενο της παρούσας μεταπτυχιακής διατριβής ειδίκευσης ήταν η μελέτη των φυσικοχημικών και μηχανικών ιδιοτήτων παθολογικά αλλοιωμένων ανθρώπινων μηνίσκων. Οι μηνίσκοι είναι ινοχόνδρινοι μηνοειδείς δίσκοι που παρεμβάλλονται μεταξύ των αρθρικών επιφανειών του μηριαίου κονδύλου και της κνημιαίας γλύνης. Αποτελούνται ως επί το πλείστον από ίνες κολλαγόνου και χονδροκύταρα. Περιέχουν ακόμα γλυκοσαμινογλυκάνες, ελλαστίνη και DNA. Συμβάλλουν καθοριστικά στη σωστή λειτουργία της άρθρωσης καθώς είναι επιφορτισμένοι με λειτουργίες, όπως η προστασία των αρθρικών επιφανειών από τη φθορά, η λίπανση και η σταθεροποίηση της άρθρωσης. Η σημαντικότερη ίσως μεταβολική νόσος των μηνίσκων είναι η χονδρασβεστίωση (chondrocalcinosis) κατά την οποία παρατηρείται ανάπτυξη ανόργανων αλάτων στην επιφάνεια ή/και στο σώμα του ιστού. Για τη μελέτη των ιδιοτήτων των παθολογικά αλλοιωμένων ανθρώπινων μηνίσκων χρησιμοποιήθηκαν τεχνικές μηχανικής και φυσικοχημικής ανάλυσης. Με Δυναμική Μηχανική Ανάλυση επιχειρήθηκε να αξιολογηθεί η επίδραση των διαφόρων τύπων παθολογικών αλλοιώσεων στις δυναμικές μηχανικές ιδιότητες των ιστών. Οι ασβεστοποιημένοι ιστοί και οι εναποθέσεις τους μελετήθηκαν με φασματοσκοπικές τεχνικές RAMAN, FT-RAMAN, FT-IR καθώς επίσης και με απεικονιστικές τεχνικές οπτικής μικροσκοπίας, σαρωτικής μικροσκοπίας ηλεκτρονίων (SEM) και μικροσκοπίας ατομικής δύναμης (AFM). Επιπλέον, για πρώτη φορά έγινε διασύγκριση της ικανότητας τριών διαφορετικών διατάξεων RAMAN (FT-RAMAN: 1064 nm, Dispersion RAMAN: 633 nm και Dispersion RAMAN: 325 nm) να εντοπίσουν την παρουσία εναποθέσεων στους ιστούς. Στην παρούσα μελέτη εξετάστηκαν 63 μηνισκικά μοσχεύματα από οστεοαρθριτικούς ασθενείς οι οποίοι υποβλήθηκαν σε ολική αρθροπλαστική γόνατος στο Περιφερειακό Πανεπιστημιακό Νοσοκομείο Πατρών. Τα μοσχεύματα αυτά εξετάστηκαν από ιστοπαθολόγο και διαγνώστηκαν οι παθολογικές αλλοιώσεις που έφερα καθένας από αυτούς. Με βάση τα ιστοπαθολογικά αποτελέσματα έγινε κατηγοριοποίηση των δοκιμίων και συσχέτιση των αποτελεσμάτων αυτών με τα αποτελέσματα των προαναφερθέντων τεχνικών. Από τα αποτελέσματα της δυναμικής μηχανικής ανάλυσης φάνηκαν μικρές αλλά όχι στατιστικά σημαντικές διαφοροποιήσεις μεταξύ ασβεστοποιημένων μηνίσκων και μηνίσκων που παρουσίαζαν μυξωματοειδή εκφυλισμό και κύστες (σύμφωνα με τα αποτελέσματα της ιστοπαθολογικής εξέτασης). Τα αποτελέσματα της περιθλασιμετρίας ακτίνων Χ των ασβεστοποιημένων ιστών έδειξαν παρουσία μείγματος τρικλινούς και μονοκλινούς διένυδρου πυροφωσφορικού ασβεστίου (CPPD) υψηλής κρυσταλλικότητας καθώς και μικροκρυσταλλικές εναποθέσεις απατιτικής σύστασης (HAP). Οι τεχνικές μικροσκοποίας (οπτική, SEM και AFM) επιβεβαίωσαν τον παραπάνω ισχυρισμό αποκαλύπτοντας το χαρακτηριστικό τρόπο ανάπτυξης κάθε τύπου εναπόθεσης. Τα αποτελέσματα της φασματοσκοπικής ανάλυσης με χρήση των τεχνικών RAMAN – FT-RAMAN – και FT-IR έδειξαν την παρουσία επίσης χαρακτηριστικών κορυφών του CPPD και του HAP. Οι εναποθέσεις από CPPD ανιχνεύθηκαν στο 82.6 % των ασβεστοποιημένων δειγμάτων (19 στα 23) ενώ από απατίτη στο 17.4% (4 στα 23) Aναφορικά με τα RAMAN, επισημαίνεται ότι ανεξάρτητα από το μήκος κύματος που χρησιμοποιήθηκε και παρά την ισχυρή μπάντα φθορισμού που επεδείκνυαν τα φάσματα, οι χαρακτηριστικές κορυφές των εναποθέσεων ήταν εμφανείς. / The aim of the present master thesis study was the physicochemical and biomechanical characterization of pathological human menisci. Menisci are semilunar shaped fibrocartilaginous tissues that lie between the femoral condyle and tibia plateau. They mainly consist of collagen fibers and chondrocytes. Additionally glycosaminoglycans, elastin and DNA are present in the tissue. Menisci play an important role in the good function of human knee joint by distributing the applied loads, contributing to the joint’s stability and lubricating the joint’s area. The most common metabolic meniscal lesion is the chondrocalcinosis where inorganic phases of calcium or sodium salts are deposited in/on the tissue. Pathologically altered human menisci were studied by mechanical and physicochemical analysis techniques. Dynamic Mechanical Analysis was used to investigate the effects of degeneration lesions on the dynamic mechanical properties of the tissue. Calcified tissues and the corresponding encrustations were studied by spectroscopic techniques such as RAMAN, FT-RAMAN, FT-IR as well as imaging techniques such as optical microscopy, scanning electron microscopy (SEM) and atomic force microscopy (AFM). Moreover, for the first time a comparison of the ability of three different RAMAN setups (FT-RAMAN: 1064 nm, Dispersion RAMAN: 633 nm and Dispersion RAMAN:325 nm) to detect calcification on the tissues was performed. The clinical sample comprised of 63 menisci with histopathologicaly confirmed alterations that were surgically extracted from osteoarthritic patients due to total knee arthroplasty in the University Hospital of Patras. Samples were classified according to the histopathological evaluation and a correlation of histopathological with mechanical or spectroscopic findings was attempted. Dynamic mechanical analysis results showed small but no statistically significant difference between the group of calcified and the group of mucoid and cystic degenerated tissues (as determined after histopathological examination) X-ray diffraction analysis of the menisci encrustations revealed highly crystallized depositions of triclinic and monoclinic Calcium Pyrophosphate Dihydrate (CPPD) and some samples with poorly crystallized apatitic depositions (HAP). Microscopic techniques (optical, SEM and AFM) confirmed the presence of CPPD and HAP and in addition revealed information regarding their growth mechanism.The results of spectroscopical analysis using RAMAN – FT-RAMAN and FT-IR showed also the characteristic peaks of the inorganic phase (CPPD or HAP) presented in/on the tissue. CPPD encrustations were detected in 82.6 % (19 of 23) of the calcified samples while apatite in 17.4 % (4 of 23). Moreover, it is notable that all RAMAN setups showed the characteristic peaks of CPPD and HAP regardless of the excitation source and the strong fluorescence band.