Precision agriculture is an agronomic approach that aims to optimize inputs into agricultural production to achieve balanced, qualitatively and quantitatively the best possible yields, while minimizing the financial costs and maintain or improve the environment. The basic premise of this approach is the knowledge of soil conditions of land used for agricultural production. This information can be found in agronomic records, soil maps or results of agrochemical testing. Indirect methods of soil heterogeneity mapping based on the principle of sensor measurements and remote sensing are also available. Indirect methods for determining the physical-chemical properties of soil are generally user-friendly, cheaper, faster, and have a higher density of measurement points than the sampling of soils. Comparison of direct and indirect methods showed that indirect methods provide a detailed picture of soil variability. However, interpretation of results is affected by many other factors, which are based in the principle of soil het-erogeneity itself.

The aim of this thesis is to design and implement modules for wireless sensor network ZigBee. These modules could communicate together and share information about measured values. The network will be managed by coordinator of network, which will process data from sensor modules and will decide about next steps around the network.

Heme-based gas sensing proteins belong to a group of proteins that are present in signalling pathways of bacteria. A precise regulation of physiological functions, such as intercellular communication or biofilm production, is essential for the survival of these bacteria and their adaptation to the changing surrounding conditions. Heme-based gas sensors are able to detect the concentration of gas molecules in the local environment via their sensory domain (which contains a heme molecule as the intrinsic detection site) and transmit the signal to the functional domain helping to regulate the adaptation of many processes. These, often pathogenic, processes contribute to extended resistance of bacteria against antibiotics. Heme-based sensors are thus potentially a new therapeutic object of interest in antimicrobial treatment. In order to provide this type of treatment, it is crucial to understand the exact mechanism of intramolecular signal transduction facilitated by heme-based sensors. One of the approaches to unravel these mechanisms is further study of model sensory proteins. This thesis focuses on the analysis of a signal transduction performed by two model globin-coupled heme-based oxygen sensors.

EN Heme containing gas sensor proteins play important role in bacterial physiology in regulating many processes such as cell differentiation, virulence, biofilm formation or intercellular communication. For their structure, typical modular architecture is characteristic where various sensor domains (usually at the N-terminus) regulate the activity of the catalytic or functional domains (usually at the C-terminus). In this dissertation thesis, we focused on three representatives from the group of oxygen sensing proteins, namely histidine kinase AfGcHK, diguanylate cyclase YddV, phosphodiesterase EcDOS and also on protein RR, which is the interaction partner of AfGcHK. The main aim of the thesis was to study intra-protein/inter-domain signal transduction in two representatives of heme sensor proteins with a globin fold of the sensor domain (AfGcHK, YddV) and in one representative with PAS fold of the sensor domain (EcDOS). Another objective was to describe inter-protein signal transduction in the two component signaling system AfGcHK-RR and structurally characterize these two interacting partners. Emphasis was also placed on the study of the interaction between model sensor domains and different signaling molecules and also on function of individual amino acids involved in the binding of these...

The diploma thesis is focused on comparison of sensored and sensorless control of 3phase BLDC motor. This diploma thesis was compiled with engineers from Honeywell, spol. s r. o. and NXP Semiconductors comapny. Theoretical part of the diploma thesis is describing princip of function BLDC motor and different methods of control. The testing of NXP sensored and sensorless application is done in the practical part. Regarding to the fact, that applications were provided by NXP, furthemore the diploma thesis additionally contains a state machine using sensorless application from NXP. Finally, the author is evaluating a cooperation with NXP company and he thinks about next possible cooperation.

The masters thesis concerns localization and its influence on other processes in a wireless network. The first part of the thesis is devoted to the study of localization algorithms in wireless sensor networks. The second and third part is devoted to the description of hierarchiacal aggragation and Greedy perimetr stateless rating (GPSR). The fourth part presents implementation of GPSR protokol into the MATLAB simulation tool. It contains the description of specific m-files, that are use for simulation. The fifth part deals with the simulation itself. And the final part presents simulation results.

The work deals with n-channel system enabling many sample analysis at sort time from sensor array using electrochemical methods. The 8x12 sensor arrays are formed from 3 electrodes system created on PCB which can be used for heavy metal analysis and toxic substances determination. The control unit switches each 3 electrode sensor to 8 channel precise potentiostat which was designed for electrochemical analysis with current sensitivity below 10 pA. The potentiostat can synchronize with control unit. Developed system is able to measure up to eight single inputs and may be simply converted to measure other nonchemical values. Measured data will be sent to user-friendly application in computer and analyzed or saved consequently.

This thesis describes processing of RSSI parameter and its subsequently use for cal- culating distance of wireless node. This thesis also describes analysis of radio model environment and calibration of key variables needed for localization. There is also sys- tem realized for localization of wireless nodes in sensor network. It uses dynamically calibrated variables for calculations, which describes radio model.

In the context of this master’s thesis are solved algorithms and hardware for control BLDC motor. The first was designed algorithm in LabView called "six-step". This algorithm causes a torque ripple, so in the final version was used vector control. It is a sensor control, so was necessary to design a measurement of rotor position. It was solved using a magnetic position sensor. After testing the control algorithm was designed my own hardware for control the BLDC motor. The main control unit was chosen microprocessor MC56F82743, which is designed specifically for BLDC motor control. The microprocessor was programmed in the CodeWarrior development environment, using a JTAF and USB-TAP.

This thesis focuses on energy efficiency of particular aspects of data processing on wireless sensor nodes and deals with methods aiming to decrease energy consumption of wireless sensor network (WSN) applications requiring high processing power. The work presents comprehensive analysis of mesh protocols and available hardware platforms in terms of energy efficiency. A new energy efficient sensor node with high processing capability is presented and utilized to evaluate the proposed system for autonomous data transmission in WSN. Finally, an implementation of real-time operation system supporting mesh communication and very strict energy requirements through energy profiles is also presented. A valuable finding is that further increase of energy efficiency is only possible through a holistic approach in software and hardware architecture design, so that hardware and software/ firmware are tightly coupled. The output of this research might be applied in industry or academy field without necessity of any change or prior knowledge of WSN. The autonomous system of data transmission opens new research possibilities to decrease energy requirements of WNS.