The objectives of this study were to determine the effects the temperature sensitivity of alleles of Rsv1 in soybean (Glycine max (L.) Merr.). Soybean cultivars carrying alleles of Rsv were exposed to 1 several heat treatments designed to induce heat shock protein production prior to inoculation with soybean mosaic virus (SMV). The heat treatment methods were similar to those employed in the research with N gene-tobacco mosaic virus studies. The soybean cultivars used were Lee 69, York, Kwanggyo, Ogden and PI96983, carrying the Rsv, Rsv1-y, Rsv1-k, Rsv1-t, and Rsv1 allles of Rsv1, respectively, and were selected to provide a range of reactions to selected SMV pathotype groups. For example Rsv1-y and Rsv1-k give a necrotic response to SMV G4 and SMV G6, respectively, while both are resistant to SMV G1. To determine the durability of resistance under heat shock conditions, the symptoms were observed for changes in the phenotype of the resistance response. Immunological techniques were employed to determine the vascular movement and localization of the viral antigen in the plant. Heat treatments used were found to induce HSP but to have no effect on the resistance phenotype. A detached leaf assay was used to test the same Rsv alleles at constant 1 high temperatures. Primary trifoliolate leaflets were removed and inoculated, then placed into a continuously lighted incubator at 20 °C or 30 °C. Leaf immunoprint assays were used to determine the localization of the viral antigen. The visible symptoms for necrotic lesions and veins were observed for necrotic phenotype-pathotype combinations but mosaic symptoms were not observed on detached leaves, as expected for inoculated leaves. The detached leaf assay confirmed that no change from the expected resistance response of the Rsv alleles occurred at 30 C. A breakdown 1 o of resistance to SMV at high temperature had been reported in soybean by Tu and Buzzell (1987). The resistance gene in which the high temperature breakdown occurred has been determined to be Rsv . Using cultivars and breeding lines carrying Rsv a similar experiment was attempted in growth 3 3 chambers. Preliminary results suggest that Rsv is temperature sensitive. / Master of Science

The four interrelated experiments reported in this thesis were designed to study the effects of previous exposure to electric shock on the subsequent conditioning and extinction of an avoidance response in rats. The purposes of the research were twofold. The first objective was to test a conflict hypothesis concerning lengthy avoidance conditioning situations. The second purpose was to investigate the ways in which preshocks used to produce such a state of conflict affected behavior. The results indicated that preshocks have an effect on subsequent avoidance conditioning only when they are paired with stimuli which occur in the avoidance situation. Certain preshock procedures which were supposed to produce conflict did in fact retard acquisition. Furthermore, response possibilities available at the time of preshocks were found to be important determinants of subsequent behavior. / Thesis / Master of Arts (MA)

The Navy is interested in comparing multi-thermocouple probes, tested in the field, with scanned laser absorption thermometry. This comparison aims to understand the effects of excess Carbon Monoxide (CO) and carbon (soot) resulting from rich nitromethane (CH3NO2) combustion events interacting with the outside air, as well as aluminum catalysts, on the temperature of the ensuing fireball. These interactions create mixing zones with varying gas temperature and composition. Currently, research at the UCF shock tube involves taking preliminary CO-scanned thermometry data with the goal of comparing thermocouple insert results in the future. The thermocouple insert is securely positioned within a specially designed end wall and protected by a heat shield. By comparing the temperature measurements obtained by one-dimensional shock relations with those obtained through scanned laser thermometry, based on CO characterization experiments conducted previously on the same shock tube, we aim to analyze temperature measurements and evaluate how the presence of the thermocouple insert affects the incident shockwave geometry, as well as the resulting reflected wave and temperature conditions. The goal is to observe any discrepancies in temperature measurements between the one-dimensional shock relations and the scanned laser method. This will enable researchers to assess the impact of the thermocouple insert in testing environments. Experiments were conducted using a mixture of 3% carbon monoxide (CO), 20% helium (He), and 77% argon (Ar), with an expected temperature range of 950 – 1950K, at pressures of 0.7 – 1 atmosphere (atm).

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