The behavior of a protein molecule at the solid-liquid interface is a worthy scientific problem for at least three reasons. The main driving force for studying this problem is a practical one, as many areas of bio-related technologies, such as medical implants, biosensing, and drug delivery, require the understanding of protein-surface interactions. In this dissertation, the nature of the precursive weakly adsorbed state of proteins during binding is reviewed. From this perspective, the adsorption and binding of proteins to a solid block copolymer thin film was achieved with regular spacing. Further efforts produced a monolayer of green fluorescent protein (GFP) covalently bound with regular spacing and orientation to a diblock copolymer thin film. This protein could be folded and refolded by changing solvent characteristics. We also explored the binding of DC-SIGN to mannose and mannotriose bearing lipid membranes. While no binding was observed, the usefulness of the lipid-based glycan microarray was proven using the well-studied CTB-GM1 binding motif. / text

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A sign pattern matrix is a matrix whose entries are from the set {+,-,0}. The minimum rank of a sign pattern matrix A is the minimum of the rank of the real matrices whose entries have signs equal to the corresponding entries of A. It is conjectured that the minimum rank of every sign pattern matrix can be realized by a rational matrix. The equivalence of this conjecture to several seemingly unrelated statements are established. For some special cases, such as when A is entrywise nonzero, or the minimum rank of A is at most 2, or the minimum rank of A is at least n - 1,(where A is mxn), the conjecture is shown to hold.Connections between this conjecture and the existence of positive rational solutions of certain systems of homogeneous quadratic polynomial equations with each coefficient equal to either -1 or 1 are explored. Sign patterns that almost require unique rank are also investigated.

Tai teorinis – praktinis darbas, kuriame nagrinėjamas, analizuojamas politinių ženklų naudojimas mene, o taip pat ir Lietuvos meninėje fotografijoje. Nagrinėjamas fotografinio portreto kaip politinio ženklo naudojimas. Taip pat svarbi teorijos dalis gilinasi į portreto bei fotografinio portreto panaudojimą mene, jį traktuojant kaip politinį ženklą. Gyvendami ženklų apsuptyje atpažįstame begales ženklų bei simbolių. Tačiau stipriausi mus veikiantys ženklai yra tie, kurie užsifiksavę mūsų atmintyje. Lengviausiai identifikuojami, dažnai matomi, kontraversiškiausi ženklai yra lengviausiai įsimenami, todėl stipriausiai veikia ne tik asmeninę, bet ir kolektyvinę pasamonę. Kūrybinio darbo objektas – fotoinstaliacija „Prarasto laiko beieškant“. Fotoinstaliacija žvelgiama į praėjusį Nepriklausomos Lietuvos laikotarpį, kurį nurodo politiniais ženklais pasirinkti Lietuvos Respublikos prezidentų portretai. Per portretus matoma kasdieninio gyvenimo akimirka iliustruoja tuo laikotrpio žmonių gyvenimą. / The thesis analyses how political signs are used in art as well as in Lithuania’s art photography. In this work photographic portrait is being analised as a political sign, which is being intentionally or accidentally used in photography. As we live surounded by signs we are able to recognize thousand of them. Easiest to remember are the ones, that has meaning based on our knowledge and past experiences. Easiest to identify are most widespread signs, the one, that you can see everyday. In the creative part of the study the photoinstalation created on the chosen theme summarize the essence of the final thesis. Works focus on portrait as a political signs usage in art.

<p>The communication barriers between deaf and hearing society mean that interaction between these communities is kept to a minimum. The South African Sign Language research group, Integration of Signed and Verbal Communication: South African Sign Language Recognition and Animation (SASL), at the University of the Western Cape aims to create technologies to bridge the communication gap. In this thesis we address the subject of whole hand gesture recognition. We demonstrate a method to identify South African Sign Language classifiers using an eigenvector ap- proach. The classifiers researched within this thesis are based on those outlined by the Thibologa Sign Language Institute for SASL. Gesture recognition is achieved in real- time. Utilising a pre-processing method for image registration we are able to increase the recognition rates for the eigenvector approach.</p>

Sign language interpreters (SLI) provide a vital service to the deaf community but also experience high levels of pain and suffer from career threatening musculoskeletal disorders. Balancing work and rest (recovery) may be a useful intervention to help address these concerns. This thesis addresses two specific questions, parsed out of a larger study seeking to determine ideal work to rest ratios for sign language interpreting. The aims of this specific body of work were to evaluate sign language interpreters (SLIs) perceptions of the mental and physical demands associated with different work to rest ratios; and, to measure kinematics during signing, comparing kinematic outcomes between novice and experienced SLIs and over time using the work to rest ratio that was perceived as the least demanding (as identified in aim 1). Nine novice and nine experienced interpreters participated in the study, each interpreting the same ten hours of a university level lecture, over the course of six visits to the laboratory. During each session interpreters worked (“hands in the air”) for 60 minutes, but used a different work to rest strategy in each session. These strategies ranged from 10-minute work, 10-minute rest, to 60 minutes of continuous work with no rest. During each session, participants were instrumented with motion capture and electromyography sensors while interpreting in a simulated working environment. In addition, SLIs were asked to provide feedback about their perceptions of the mental and physical demands associated with each session. The first study in this thesis reports on participant’s subjective feedback about the six different sessions (paper #1); where participants identified the 15-minute work to rest ratio as ideal. The second paper reports on kinematic data from this perceived ideal work to rest ratio (paper #2). Despite interpreting in a work to rest ratio that SLIs perceived as ideal, kinematic variables with known associations to injury risk (joint position, velocity, micro-breaks) readily exceeded reported thresholds. / Thesis (Master, Kinesiology & Health Studies) -- Queen's University, 2014-05-29 17:46:11.133

Video communication of British Sign Language (BSL) is important for remote interpersonal communication and for the equal provision of services for deaf people. However, the use of video telephony and video conferencing applications for BSL communication is limited by inadequate video quality. BSL is a highly structured, linguistically complete, natural language system that expresses vocabulary and grammar visually and spatially using a complex combination of facial expressions (such as eyebrow movements, eye blinks and mouth/lip shapes), hand gestures, body movements and finger-spelling that change in space and time. Accurate natural BSL communication places specific demands on visual media applications which must compress video image data for efficient transmission. Current video compression schemes apply methods to reduce statistical redundancy and perceptual irrelevance in video image data based on a general model of Human Visual System (HVS) sensitivities. This thesis presents novel video image coding methods developed to achieve the conflicting requirements for high image quality and efficient coding. Novel methods of prioritising visually important video image content for optimised video coding are developed to exploit the HVS spatial and temporal response mechanisms of BSL users (determined by Eye Movement Tracking) and the characteristics of BSL video image content. The methods implement an accurate model of HVS foveation, applied in the spatial and temporal domains, at the pre-processing stage of a current standard-based system (H.264). Comparison of the performance of the developed and standard coding systems, using methods of video quality evaluation developed for this thesis, demonstrates improved perceived quality at low bit rates. BSL users, broadcasters and service providers benefit from the perception of high quality video over a range of available transmission bandwidths. The research community benefits from a new approach to video coding optimisation and better understanding of the communication needs of deaf people.

Students who are deaf and use sign language frequently have language delays that affect their literacy skills. Students who use American Sign Language (ASL) often lack fluent language models in both the home and school settings, delaying both the development of a first language and the development of literacy in printed English. Mediated and scaffolded instruction presented by a More Knowledgeable Other (MKO; Vygotsky, 1978, 1994) may facilitate acquisition of a first foundational language. Repeated viewings of fluent ASL models on DVDs paired with adult mediation has resulted in increases in vocabulary skills for DHH students who used ASL (Cannon, Fredrick, & Easterbrooks, 2010; Golos, 2010; Mueller & Hurtig, 2010). Classifiers are a syntactic sub-category of ASL vocabulary that provides a critical link between ASL and the meaning of English phrases. The purpose of this study was to investigate the effects of teacher-mediated repeated viewings of ASL stories on DHH students’ classifier production during narrative retells. This study included 10 student participants in second, third, and fourth grades and three teacher participants from an urban day school for students who are DHH. The researcher used a multiple baseline across participants design followed by visual analysis and calculation of the percentage of non-overlapping data (PND; Scruggs, Mastropieri, & Casto, 1987) to examine the effects of the intervention. All students increased their classifier production during narrative retells following a combination of teacher mediation paired with repeated viewings of ASL models.

Typescript. / Thesis (Ph. D.)--University of Hawaii at Manoa, 1986. / Bibliography: leaves 296-314. / Photocopy. / Microfilm. / xii, 314 leaves, bound 29 cm

Doctor of Philosophy (Phd) / Despite an abundance of evidence demonstrating that the temporal relationship between events is a key factor in an organism learning an association between those events, a general theoretical account of temporal contiguity has remained elusive. A particular question that has received little attention is whether behaviour established with strong contiguity can be maintained when contiguity is weakened. The primary aims of this thesis were to examine the mechanisms underlying both the effects of contiguity on learning in rats and humans and the maintenance effect described above. The experiments reported in this thesis demonstrated that rats’ lever-pressing for food/sucrose acquired with immediate reinforcement persisted when a trace/delay that would have prevented acquisition was subsequently introduced, provided the lever was a valid signal for reinforcement. In classical conditioning with a 10-second trace, rats performed magazine-entry during lever-insertion (goal-tracking) instead of lever-pressing (sign-tracking); with zero-trace, rats both sign- and goal-tracked if lever-insertion time was 10 seconds, while goal-tracking dominated with 5-second lever-insertion time. Furthermore, while it was found that context-US associations may interfere with CS-US learning, context conditioning did not contribute to the retardation of sign-tracking in trace conditioning. Overall, these results are consistent with the theory that a localisable manipulandum that signals an appetitive outcome with strong contiguity acquires hedonic value, and that such hedonic value drives lever-pressing behaviour that is resistant to changes in the conditions of reinforcement. Human performance in a conditioned suppression task was inversely related to trace interval, but this apparent contiguity effect was at least partially mediated by the number of distractors during the trace interval, as predicted by Revusky’s concurrent interference theory. Furthermore, some transfer of conditioned suppression was observed when the trace was subsequently lengthened. Despite the different explanations proposed to account for rat and human performance in these experiments, the results suggest that the effects of contiguity on learning may be driven by similar underlying mechanisms across species.