The purpose of this project was to produce signal relay stations that could receive information via 2.4 GHz radio and relay the information to a designated target station. If a relay station was located outside of signal range for the target station it was supposed to utilize other relay stations to transfer the information to the target station, so called multi-hop. The would-be application of the relay stations was orienteering. When an orienteer punches a checkpoint the signal stations would relay information of who punched the control, when it was punched and the checkpoint that was punched to the speaker tower in the goal area. The work resulted in prototypes which fulfilled the statement of purpose and was tested at an orienteering competition with satisfactory results. The performance of the prototypes was tested and found adequate for the would-be application.

Target of Rapamycin Complex 2 (TORC2) has conserved roles in regulating cytoskeleton dynamics and cell migration and has been linked to cancer metastasis. However, little is known about the mechanisms regulating TORC2 activity and function in any system. In Dictyostelium, TORC2 functions at the front of migrating cells downstream of the Ras protein RasC, controlling F-actin dynamics and cAMP production. Here, we report the identification of the small GTPase Rap1 as a conserved binding partner of the TORC2 component RIP3/SIN1, and that Rap1 positively regulates the RasC-mediated activation of TORC2 in Dictyostelium. Moreover, we show that active RasC binds to the catalytic domain of TOR, suggesting a mechanism of TORC2 activation that is similar to Rheb activation of TOR complex 1. Dual Ras/Rap1 regulation of TORC2 may allow for integration of Ras and Rap1 signaling pathways in directed cell migration.