Spelling suggestions: "subject:"sizeexclusion chromatography"" "subject:"size.conclusion chromatography""
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Synthesis and Characterization of Arborescent (Dendritic) Polystyrenes Prepared by Raft PolymerizationHeidenreich, Andrew J. 10 August 2011 (has links)
No description available.
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The use of SEC-UV in formulation optimization for a protein-peptide conjugate drug candidateImedashvili, Sumay January 2024 (has links)
Many companies, including Strike Pharma, are developing biologicals for individualized immunotherapeutic cancer treatments. The possibility to combine a bispecific antibody with a myriad of endogenous antigenic peptides opens the doors for highly personalized therapies. Setting up and using analytical assays is key to evaluate aggregation and overcome aggregation patterns of biologicals during CMC development. The aim of this project was to assess size exclusion chromatography (SEC) as an analytical method and subsequently evaluate several drug formulations that could be suitable for subcutaneous administration of a peptide and antibody conjugate mix. The formulations were based on a 25 mM histidine buffer pH 6.0, that had been optimized for the antibody alone, with different additives. By utilizing SEC coupled to UV-detection at 280 nm, aggregates were detected and quantified. The most effective excipients were dimethyl sulfoxide, polyethylene glycol 400 and arginine. Two different peptide-tags were compared and the pTag9mer-mut2 variant was more favorable than pTag9mer-mut1 in limiting aggregate formation with highest success rates at 1.5 mg/mL protein concentrations and the fulfillment of the high molecular weight ≤ 5% criterion. Combining antibody and peptide containing pTag9mer-mut1 in a pH 9.0 histidine buffer with added arginine engendered the least aggregates compared to any pH 6.0 formulation. However, the instability of the antibody in pH 9.0 and the risk of deamidation makes this less suitable. Future considerations include changing the administration method or using pump injection strategy, which allows higher injection volumes to limit aggregation by lowering protein concentrations.
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Temperature Dependent Size Exclusion Chromatography for Investigating Thermoreversibly Bonding Polymer SystemsBrandt, Josef 01 August 2016 (has links) (PDF)
Polymers capable of thermally controlled reversible bonding reactions are promising candidates for stimuli responsive materials, as required for self-healing or drug delivery materials. In order to investigate how the dynamic reactions can be controlled, effective analytical tools are demanded that are capable of analyzing not only the polymers but can also monitor the respective bonding reactions. Herein, we employ size exclusion chromatography in a newly developed temperature dependent mode (TD SEC) for the in situ characterization of polymers that undergo retro Diels-Alder (rDA) reaction at temperatures higher than 60 °C. Monitoring the evolution of the molar mass distribution of the polymers during the rDA reaction and evaluating the data quantitatively gives detailed information about the extent of the reaction and allows elucidating structural parameters that can be used for controlling the polymers debonding behavior.
In contrast to spectroscopic techniques, TD SEC analyzes only the size of the polymers, hence the polymers do not need to fulfill any particular requirements (e.g. presence of detectable functional groups) but only need to be soluble in the TD SEC, which makes the method universally applicable. Side effects that might bias the results are minimized by using a high temperature chromatograph that allows performing the analysis in a broad temperature range (60 – 200 °C) and in different solvents. Thus, the analysis can be performed under the exact conditions that are required for the bonding reactions and an in situ image is provided.
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Dextranas em açúcares e em aguardentes de cana / Dextran in sugar and in sugar cane spiritsAquino, Francisco Wendel Batista de 11 August 2009 (has links)
O Brasil é atualmente o maior produtor e exportador mundial de açúcar e a aguardente de cana aqui produzida é a terceira bebida destilada mais consumida no mundo. Contudo, estes produtos ainda têm a sua qualidade afetada pela presença de Dextranas. Devido à sua origem (produto secundário do metabolismo bacteriano) e por conta das suas propriedades físico-químicas, as dextranas constituem um importante meio para a avaliação da qualidade do açúcar. No âmbito industrial, as dextranas podem causar diversos problemas ao setor alimentício que utiliza o açúcar como matéria-prima, além de estarem diretamente relacionadas à formação de precipitados na aguardente de cana adoçada. Este trabalho apresenta o primeiro perfil da distribuição de massa molar das dextranas presentes no açúcar brasileiro em função dos seus valores de Mn, Mw, Mz e polidispersividade, os quais exercem influência significativa sobre suas características físico-químicas. Estes parâmetros foram determinados via cromatografia líquida de exclusão por tamanho. O perfil de distribuição de massa molar traçado exibiu, majoritariamente, a presença de dois grupos de dextranas com valores de Mw médios de 5,0 x 106 e 4,8 x 104 Da, e, ocasionalmente, a presença de dextranas que foram classificadas num terceiro grupo com massas molares da ordem de 105 Da. Através deste perfil, foram avaliadas, em sistemas modelo aguardente-dextrana, as influências dos fatores temperatura, acidez, presença dos íons metálicos CuII, FeIII, CaII e MgII e incidência de luz sobre a velocidade de formação dos depósitos de dextranas na aguardente de cana. Demonstrou-se que a acidez e a temperatura foram os fatores que mais influenciam na velocidade de precipitação das dextranas em aguardentes de cana adoçadas. / Brazil is the largest producer and exporter of sugar in the world, and the cachaça is the third most produced distilled beverage in the world. However, these products have their quality affected by the presence of dextrans. Because of its origin (a secondary compound of bacterial metabolism) and due to its physical-chemical properties, the dextrans are an important indicator to assess the sugar quality. Dextrans can lead to problems in processed food production, and it has a strong relation with insoluble deposits in sugared sugar cane spirits. The dextran molecular mass distribution profile in terms of Mn, Mw Mz and polydispersity in Brazilian sugars are reported for the first time. The analyses were accomplished by Size-exclusion chromatography, using a refraction index detector. In most of the sugar samples, it was possible to identify two major groups of dextrans with Mw averages of 5.0 x 106 e 4.8 x 104 Da. A third group of dextrans with Mw of 105 was occasionally observed. With this data, model systems were built to assess the influence of the temperature, acidity, presence metalic ions CuII, FeIII, CaII, and MgII, light incidence in relation of dextran precipitation velocities. It is demonstrated that factors temperature and acidity are the most influential in the dextran precipitation velocity in sugar cane spirits.
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Nouvelle méthodologie pour la caractérisation de distributions de masses molaires d'échantillons cellulosiques complexes / New methodology for the caracterization of molar mass distributions of complex cellulosic samplesRebiere, Jérémy 20 March 2017 (has links)
La cellulose est un biopolymère naturel et très abondant. Selon son origine et son mode d’extraction, elle présente des propriétés de cristallinité et de longueur de chaînes variables. Les interactions hydrogène entre les chaînes de cellulose sont en grande partie responsables de son organisation et forment un réseau très dense qui limite sa solubilité. Sa dissolution, complète et non-dégradante, est donc compliquée et dépend de la capacité du solvant à rompre ces liaisonshydrogène intermoléculaires. L’analyse de la cellulose par chromatographie d’exclusion stérique (SEC) pour suivre l’évolution des distributions de masses molaires (DMM) au cours des procédés de transformation est donc toujours problématique. Le système de solvant le plus commun estactuellement le chlorure de lithium/N,N-diméthylacétamide (LiCl/DMAc) qui est extrêmement toxique. L’objectif de ce travail est le développement d’une méthode d’analyse des DMM de la cellulose dans des conditions moins toxiques et adaptée à tous les types de cellulose. Parmi les nombreux systèmes de solvant décrits dans la littérature, 3 systèmes, plus verts et nondérivatisants, ont été sélectionnés afin d’identifier un système de solvant permettant la dissolution,sans dégradation, d‘échantillons de cellulose de cristallinité et de masse molaire moyenne variables afin de remplacer LiCl/DMAc. Les analyses thermogravimétriques et les mesures viscosimétriques ont permis d’évaluer et de comparer les modifications de 4 échantillons de cellulose dissous dans ces 3 systèmes et dans le LiCl/DMAc. LiCl/DMAc dégrade les celluloses de plus haute masse molaire (-cellulose et Vitacel), réduisant de 50 % les longueurs de chaîne après dissolution. Le système tétrabutylammonium/diméthylsulfoxide (TBAF/DMSO) permet une dissolution rapide des 4 échantillons de cellulose sans dégradation ou modification majeure. Le système TBAF/DMSO a, par la suite, été privilégié pour la caractérisation des échantillons en SEC. Du fait d’interactions possibles entre les groupements aromatiques composant la phase stationnaire et les molécules de TBAF, le système complet n’a pas pu être utilisé comme éluant. L’éluant choisi a donc été le DMSO mais qui, seul, ne solubilise pas les échantillons cellulosiques. Les molécules de TBAF sont cependant indispensables au mécanisme de dissolution et laconcentration en TBAF dans le DMSO a dû être adaptée en fonction des échantillons. Pour les échantillons cellulosiques de faible masse molaire, une concentration en TBAF de l’ordre de 1 %(m/v) est suffisante et permet de réaliser une analyse des masses molaires satisfaisantes. Pour les échantillons de plus haut poids moléculaire, cette concentration n’est plus suffisante pour les dissoudre convenablement. Or, avec des concentrations plus élevées, des phénomènesd’agrégation provoque l’élution d’une partie importante des macromolécules dans le volume mort, comme observé avec l’analyse de standards de pullulane. / Cellulose is a very abundant natural biopolymer. According to its origin and to its extraction mode, it presents various cristallinity rate and molar mass. Its organization relies mainly on intermolecular hydrogen bonds that form a strong network and thus limitating cellulose solubility. Complete andnon-degradative dissolution is then complicated and depends on the solvent ability to disrupt these hydrogen bonds. Analysis of the molar mass distribution (MMD) of cellulose by size exclusion chromatography (SEC) of cellulose is consequently problematic while the study of the evolution of cellulose molar mass during transformations could be extremely useful in many processes. The most common solvent used in SEC is lithium chloride/N,N-dimethylacetamide (LiCl/DMAc), which is extremely toxic. The aim of this PhD study is then to develop a new analytical method to characterize cellulose MMD using safer solvents and adapted to all kinds of cellulosic sample. Among the numerous non-derivatizing solvent systems described in the literature, three of themhave been selected. Greener and less toxic than LiCl/DMAc, their ability to dissolve cellulosic sample of various cristallinity and average molar mass without degradation was then tested. Thermogravimetric analyses and viscosimetric studies allowed to evaluate and to compare the modifications involved by the dissolution for four different cellulosic samples. LiCl/DMAc degraded the samples of higher molar mass (-cellulose and Vitacel) decreasing their degree ofpolymerization by 50 % after dissolution. Tetrabutylammonium/dimethylsulfoxide (TBAF/DMSO) system allows rapid dissolution of the 4 cellulose samples, without major degradation or modification. TBAF/DMSO system was then studied as solvent for SEC analysis of these cellulose samples. Due to the interactions between the aromatic groups composing the stationary phase with TBAF molecules, the complete system could not be used as eluant. Chosen eluant was then DMSO alone. However, as the TBAF molecules are mandatory for the dissolution of cellulose, TBAF concentration was adapted according to the cellulose nature for the preparation of the samples. For low molar mass cellulose samples, a TBAF concentration of 1 %(w/v) was sufficient and allowed to performed correctly the chromatographic analysis. For the samples of higher molar mass, this concentration was not high enough to complete the dissolution. Using, higher concentrations caused aggregation phenomena resulting in the elution of a large amount of the macromolecules in the dead volume, as observed with the analysis of pullulan standards.
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Cellulose degradation in pulp fibers studied as changes in molar mass distributionsBerggren, Rickard January 2003 (has links)
In this thesis, size-exclusion chromatography (SEC) of woodpolymers dissolved in lithium chloride/N,N-dimethylacetamide(LiCl/DMAc) has been used to characterize the molar massdistributions (MMD) of wood polymers in pulp fibers afterchemical degradation. Characterization of birch kraft pulps subjected to ozonedegradation and acid hydrolysis, respectively, rendereddifferent changes in the MMD. Ozone degradation resulted inlarge redistributions of the original MMD, observed as thedevelopment of a distinct fraction of cellulose withintermediate molar mass. Acid hydrolysis resulted in minorchanges of the original MMD compared to ozonation. Fiberssubjected to acid hydrolysis were considerably weaker thanozonated fibers. These results indicated that there aredifferences in how the two chemicals degrade the fiber. The solubility of softwood kraft pulp fibers was enhanced byderivatization of the fiber polymers with ethyl-isocyanateduring simultaneous dissolution in LiCl/DMAc. Thederivatization made it possible to achieve reliable estimationsof the MMD, and hence molar masses, of softwood kraft pulps.The derivatization procedure made it possible to dissolve 90 %of softwood kraft pulps with kappa numbers over 50. Severe alkaline degradation of birch and Norway spruce woodchips was studied both by varying the pulping time and byvarying the initial alkali concentration. Differences werefound in the MMD of the two fiber types, and the alkalinedegradation was found to affect polymers in the entire MMD. Multi-angular laser light scattering (MALLS) was used as adetection technique with SEC on cellulosic samples. The MMD andaverage molar masses obtained through directstandardcalibration with commercial standards were compared with MMDand molar masses as obtained by MALLS-detection. Largediscrepancies were found, and two methods of correcting forthese discrepancies were developed. Theoretical simulations of polymer degradation wereperformed. Random, or homogeneous degradation was used as amodel for alkaline cellulose chain scission, and a resemblancewith experimental data was observed. End-wise depolymerizationof cellulose was also simulated and the results are discussedin the light of experimentally observed MMD. <b>Keywords:</b>cellulose, kraft pulp, birch, spruce,ozonation, acid hydrolysis, degradation, MMD, sizeexclusionchromatography, light scattering, molar mass, chainscission
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Cellulose degradation in pulp fibers studied as changes in molar mass distributionsBerggren, Rickard January 2003 (has links)
<p>In this thesis, size-exclusion chromatography (SEC) of woodpolymers dissolved in lithium chloride/N,N-dimethylacetamide(LiCl/DMAc) has been used to characterize the molar massdistributions (MMD) of wood polymers in pulp fibers afterchemical degradation.</p><p>Characterization of birch kraft pulps subjected to ozonedegradation and acid hydrolysis, respectively, rendereddifferent changes in the MMD. Ozone degradation resulted inlarge redistributions of the original MMD, observed as thedevelopment of a distinct fraction of cellulose withintermediate molar mass. Acid hydrolysis resulted in minorchanges of the original MMD compared to ozonation. Fiberssubjected to acid hydrolysis were considerably weaker thanozonated fibers. These results indicated that there aredifferences in how the two chemicals degrade the fiber.</p><p>The solubility of softwood kraft pulp fibers was enhanced byderivatization of the fiber polymers with ethyl-isocyanateduring simultaneous dissolution in LiCl/DMAc. Thederivatization made it possible to achieve reliable estimationsof the MMD, and hence molar masses, of softwood kraft pulps.The derivatization procedure made it possible to dissolve 90 %of softwood kraft pulps with kappa numbers over 50.</p><p>Severe alkaline degradation of birch and Norway spruce woodchips was studied both by varying the pulping time and byvarying the initial alkali concentration. Differences werefound in the MMD of the two fiber types, and the alkalinedegradation was found to affect polymers in the entire MMD.</p><p>Multi-angular laser light scattering (MALLS) was used as adetection technique with SEC on cellulosic samples. The MMD andaverage molar masses obtained through directstandardcalibration with commercial standards were compared with MMDand molar masses as obtained by MALLS-detection. Largediscrepancies were found, and two methods of correcting forthese discrepancies were developed.</p><p>Theoretical simulations of polymer degradation wereperformed. Random, or homogeneous degradation was used as amodel for alkaline cellulose chain scission, and a resemblancewith experimental data was observed. End-wise depolymerizationof cellulose was also simulated and the results are discussedin the light of experimentally observed MMD.</p><p><b>Keywords:</b>cellulose, kraft pulp, birch, spruce,ozonation, acid hydrolysis, degradation, MMD, sizeexclusionchromatography, light scattering, molar mass, chainscission</p>
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Human copper ion transfer : from metal chaperone to target transporter domainNiemiec, Moritz Sebastian January 2015 (has links)
Many processes in living systems occur through transient interactions among proteins. Those interactions are often weak and are driven by small changes in free energy. Due to the short-living nature of these interactions, our knowledge about driving forces, dynamics and structures of these types of protein-protein heterocomplexes are though limited. This is especially important for cellular copper (Cu) trafficking: Copper ions are essential for all eukaryotes and most bacteria. As a cofactor in many enzymes, copper is especially vital in respiration or detoxification. Since the same features that make copper useful also make it toxic, it needs to be controlled tightly. Additionally, in the reducing environment of the cytosol, Cu is present as insoluble Cu(I). To circumvent both toxicity and solubility issues, a system has evolved where copper is comforted by certain copper binding proteins, so-called Cu-chaperones. They transiently interact with each other to distribute the Cu atoms in a cell. In humans, one of them is Atox1. It binds copper with a binding site containing two thiol residues and transfers it to other binding sites, mostly those of a copper pump, ATP7B (also known as Wilsons disease protein). My work was aimed at understanding copper-mediated protein-protein interactions on a molecular and mechanistic level. Which amino acids interact with the metal? Which forces drive the transfer from one protein to the other? Using biophysical and biochemical methods such as chromatography and calorimetry on wild type and point-mutated proteins in vitro, we found that the copper is transferred via a dynamic intermediate complex that keeps the system flexible while shielding the copper against other interactions. Although similar transfer interactions can be observed in other organisms, and many conclusions in the copper field are drawn from bacterial and yeast analogs, we believe that it is important to investigate human proteins, too. Not only is their regulation different, but also only in humans we find the diseases linked to the proteins: Copper level regulation diseases are to be named first, but atypical copper levels have also been linked to tumors and amyloid dispositions. In summary, my observations and conclusions are of basic research character and can be of importance for both general copper and human medicinal research.
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Starch microstructure and functional properties in waxy rice (Oryza sativa L.)Rosa Cuevas Unknown Date (has links)
Rice starch contains two types of glucose polymers, mainly linear amylose and hyper-branched amylopectin. Waxy rice has been characterised by the lack of amylose, the proportion of which being one of the most important parameters measured for rice quality. Germplasm collection work conducted in the Lao People’s Democratic Republic has previously demonstrated the diversity of this type of rice in terms of quality. The definition of a waxy rice variety is dependent on the meaning of amylose. The conventional method for determining amylose content has shown that waxy rice could have up to 5% amylose. However, including a 0% amylose standard in the standard curve causes the amylose content of these varieties to become 0-2%. In this work, the absence of amylose in waxy rice has been determined through three different approaches. Granule-bound starch synthase I (GBSS1), the enzyme which synthesises amylose, was not detected in waxy rice. Long linear chains associated with amylose were also not detected by size exclusion chromatography (SEC). The absence of these long chains affected functional properties of waxy rice, as indicated by the differences in viscosity curves between waxy and non-waxy rice. Moreover, these waxy varieties themselves exhibit differences in their viscosity curves, another indication of the diversity in coking properties in these varieties. A new approach, the ‘lnP(N) technique’, in analysing chain length distributions was applied to varieties with known mutations in two of the enzymes involved in the synthesis of amylopectin, and in determining gelatinisation temperature. It was determined that the presence of a novel feature, an interruption to linearity at DP 18-24, of the lnP(N) plot was found in rice samples with mutations in the alk gene, which codes for starch synthase (SS) IIa, and in samples with inactive branching enzyme (BE) IIb. Single nucleotide polymorphisms (SNPs) in the gene coding for SSIIa have been associated with lowered gelatinisation temperature. On the other hand, non-functionality of BEIIb changes the amylopectin structure such that gelatinisation temperature is increased. The novel feature of the lnP(N) plot is found when either or both SSIIa and BEIIb are non-functional. Waxy rice starch has hot-water-soluble (HWS) and insoluble (HWI) components. It has been confirmed that the soluble polysaccharides are structurally different from phytoglycogen, and are similar to amylopectin. Structural differences between the two fractions, which can account for their differences in solubility, were determined. At the level of the chain length distribution, the HWI fraction contained long chains not found in the HWS fraction. Considerable amounts of sucrose and glucose were found in the HWS fraction. At another level of structure, the degree of branching of the HWS components was higher than in the HWI fraction. On the other hand, the whole molecules of the HWS fraction were smaller than those of the HWI fraction. These structural differences between the two fractions potentially affected their physical behaviour, particularly solubility. The amount of leached material appears to be a property of the method, as varying cooking conditions changed the amount of HWS components. This amount reaches equilibrium at certain conditions, indicating the limited amount of the HWS material. On the other hand, the HWI component contains molecules that are insoluble in water, rather than molecules that solubilise slowly. Given the stability of the HWS fraction in solution, the ratio of the HWS fraction to the HWI fraction could potentially be used in measuring quality if the amount of the HWS fraction is variety-specific. However, the quantity of the HWS fraction appears to be a feature of the starch, rather than of the variety. Nevertheless, the fact that the soluble fraction is structurally and thermodynamically different from the insoluble fraction could presumably be grounds to classify the soluble component as a group of molecules distinct from amylopectin.
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Síntese e caracterização de géis para cromatografia de exclusão por tamanho via reticulação de Acetato de Celulose com 4,4' - Difenilmetano Diisocianato (MDI)Rosa, Wesley de Oliveira 28 March 2016 (has links)
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Previous issue date: 2016-03-28 / Não recebi financiamento / The need to obtain biomaterials in order to reduce environmental impacts has been the focus of research groups in recent years, and cellulose, a dominant component at most forms of plants is a promising resource because of its abundance. In order to improve the ability processing, the chemical modification of cellulose has been widely studied. Among the most important reactions of cellulose are: etherification, esterification, acetylation and oxidation; being cellulose acetate, viscose, nitrocellulose and cellulose ethers, the main cellulose derivatives. The chemical modification with isocyanates presents some unique properties, such as absence of by-products and chemical stability of the urethane group. In this work we were synthesized gels obtained by modified cellulose acetate (CA) with a degree of substitution (DS) 2,5 by crosslinking, with 4,4' - Diphenylmethane diisocyanate (MDI) in stoichiometry of 1:1, in homogeneous by varying the humidity and the homogenization time. For characterization were used the following techniques and tests: vibrational infrared absorption spectroscopy (Fourier Transform Spectrometer - FTIR), size exclusion Chromatography (SEC), molecular absorption spectrophotometry UV-VIS, density determining of the gels by pycnometry, determination of the coefficient swelling, determination of cross-links by Flory-Rehner theory, thermogravimetry (TG) and scanning electron microscopy (SEM). Crosslink density results showed that the gel synthesized in the absence of moisture suffered greater crosslinking with an average number of repeat units between the crosslinking points of about 1000 times lower. The potential applications of these gels were tested, by using than as stationary phase in size exclusion chromatography, having been assessed its efficiency in the fractionation and separation of natural and synthetic polymers. Results showed the effectiveness of the gel as stationary phase on separation of polymers, opening up a range of opportunities, taking into consideration the simplicity of the process and lower costs attributed to it. / A necessidade de se obter biomateriais na tentativa de reduzir impactos ambientais tem sido o foco de grupos de pesquisa nos últimos anos e, a celulose, um componente dominante na maioria das formas de plantas, é um recurso promissor devido à sua abundância. A fim de melhorar a capacidade de processamento, a modificação química da celulose tem sido amplamente estudada. Dentre as reações mais importantes da celulose estão: eterificação, esterificação, acetilação e oxidação; sendo o acetato de celulose, viscose, nitrocelulose e éteres de celulose, os principais derivados da celulose. A modificação química com isocianatos apresenta algumas propriedades únicas, como ausência de produtos secundários e estabilidade química do grupo uretano. Nesse trabalho foram sintetizados géis obtidos por meio da modificação de Acetato de Celulose (AC) com grau de substituição (GS) 2,5 através da reticulação com 4,4' - Difenilmetano Diisocianato (MDI), na estequiometria 1:1, em meio homogêneo, variando a umidade e o tempo de homogenização. Para caracterização foram utilizadas as seguintes técnicas e ensaios: espectroscopia vibracional de absorção no infravermelho por Transformada de Fourier (FTIR), cromatografia de exclusão por tamanho (SEC), espectrofotometria de absorção molecular UV-VIS, determinação de densidade dos géis por picnometria, determinação do coeficiente de intumescimento, determinação de ligações cruzadas pela teoria de Flory-Rehner, termogravimetria (TG) e microscopia eletrônica de varredura (MEV). Resultados da densidade de ligações cruzadas mostraram que o gel sintetizado na ausência de umidade sofreu uma maior reticulação, com um número médio de unidades de repetição entre os pontos de reticulação cerca de 1000 vezes menor. As aplicações potenciais desses géis foram testadas como fase estacionária em cromatografia de exclusão por tamanho, tendo sido avaliada sua eficiência no fracionamento e separação de polímeros naturais e sintéticos. Resultados mostraram a eficácia do gel como fase estacionária na separação de polímeros, abrindo uma gama de oportunidades, levando-se em consideração a simplicidade do processo e os baixos custos a ele atribuídos.
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