During college, students establish nutritional habits that may last a lifetime. Fruit and vegetable consumption often decreases during the first few years of college and the vast majority of college-­‐aged students are not meeting current recommendations. Social norms theory has been shown to be an effective method for influencing behaviors in this population. This study was designed to test whether or not providing students with normative and manipulated social norms feedback could influence fruit and vegetable intakes among college-­‐aged students as evidenced by changes in skin carotenoid levels and food frequency questionnaire reports. Participants (n=244) were randomly assigned to a control group or given either normative feedback or manipulated social norms feedback regarding their skin carotenoid levels in comparison to their peers, with carotenoid levels being an objective measurement of fruit and vegetable intake. Those receiving manipulated feedback were given an artificially low carotenoid score implying that peer consumption was greater than their own. Results indicated no significant within-­‐participant changes in fruit and vegetable intakes reported in the FFQ (P=.635). While there was no change in the control groups skin carotenoid levels (P=.996), there was a borderline significant increase among those receiving normative feedback (P=.066) and a significant increase among those receiving manipulated social norms feedback (P<.001). Repeated measures of analysis showed that within-­‐participant increases in carotenoid scores were dependent on group assignment (P=.033) with an effect size of η2p=.026 which according to Cohen’s guideline is a small effect size. The distribution of carotenoid scores and FFQ results were approximately normal. Comparisons between FFQ results and skin carotenoid levels found Pearson correlation coefficients of .301 (P<.001) and other positive correlations were found between skin carotenoid levels and both exercise and BMI (.111, P=.049; -­‐.253, P<.001). The results of this study suggest that manipulated social norms feedback can increase skin carotenoid levels. The observed increases may indicate higher carotenoid containing fruit and vegetable consumption. These findings imply that social norms feedback may potentially be used as a strategy to promote and influence greater fruit and vegetable consumption among college students.

It has been well documented that fruit and vegetable (F/V) intake is linked to lower risk of mortality and chronic disease. Raman resonance spectroscopy is a valid indicator of F/V intake and quantifies that intake by measuring skin carotenoid levels. In this study, 46 children, participated in a 6-week feeding study wherein they were randomly assigned to one of two groups: 1) consuming a high-carotenoid (HC) breakfast/snack food, or 2) consuming a placebo bar, every day. The HC food contained 4.3mg carotenoids per serving and the placebo contained none. Skin carotenoids were measured every two weeks using a BioPhotonic scanner. The treatment group had a mean increase in scanner score of 5,802 Raman intensity units which was significantly higher than the mean increase of the placebo group, 1,771. In this study we found that consumption of 120 gm of a high-carotenoid food significantly increased skin carotenoid levels in children ages 5-18 over a 6 week period. Packaging type as well as storage conditions play a role in preserving carotenoids which are sensitive to light, temperature, and oxygen. Care must be taken when choosing packaging and storage conditions for foods containing carotenoids. Two shelf-life studies, one at room-temperature and another in frozen storage, were conducted on the HC breakfast/snack food to determine the best method of packaging to maintain quality and preserve carotenoids. The food was randomly packed into one of three packaging types for both studies. The packages used in the frozen study were then randomly assigned to one of three freezer storage methods. Room-temperature samples were analyzed on days 3, 7, 10, and 14 and samples in frozen storage were pulled every month for 5 months. Measures of water activity, moisture content, color values (L*a*b*, chroma, and hue), and carotenoid content were analyzed in each sample. At the conclusion of each study, L*, b*, chroma, and hue were significantly affected by packaging type. No significant associations were found in any other measures. From this study we draw the conclusion that cellophane packaging or packaging with a N2 backflush would be the best options for use with this food to best retain its quality.

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