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Synthetic aperture radar imaging is an effective tool for imaging the sea surface because of its response to changes in sea surface roughness. This allows for the remote sensing of features on the sea surface, which modulate se surface roughness. In this work, 18 synthetic aperture radar images were collected from the TerraSAR-X and RADARSAT-2 satellites in the Port Everglades, Florida area. In-situ measurements were collected in conjunction with the satellite images in order to provide more information on the features visible in the imagery, and aid in identification of the origin of the features. Information on ships in the area of the satellite image footprints was collected using an automatic information system. Weather conditions were recorded by a meteorological station and a National Oceanic and Atmospheric Administration weather radar station. Waves and currents in the observational area were recorded with acoustic Doppler current profilers and wave gauges. Sonar systems and conductivity, depth, and salinity profilers were used to identify stratification in the water column. Surfactant release experiments were also conducted to explore the affects of surface active materials. Results of the experiment show the manifestation of atmospheric effects, oceanic fronts and eddies, wind shadowing, natural and artificial slicks, and ships and ship wakes on the synthetic aperture radar imagery. Atmospheric conditions were found to play a significant role in the visibility of features on the sea surface, and sometimes masked the appearance of features on the ocean surface. Overall the most reliable feature capable of being imaged on the sea surface by the synthetic aperture radar satellites was the signatures of ships and their wakes.

Chapter 2 The sea surface microlayer is a biogenic thin layer, comprising less than one millimeter of the ocean surface. This surface layer has gained much attention due to its dampening effect on ocean capillary ripples. The chemistry of the air-sea interface has been studied for decades; however, the structure and function of the marine bacterial community within the sea surface microlayer are still understudied. Although various sea surface microlayer sampling techniques were developed over the past decades, aseptic bacterial sampling in the open ocean is a rather challenging task. In this study, a new approach is presented. It is designed for bacterial sampling of the sea surface microlayer, which intends to reduce sampling contamination from the vessel, subsurface water and the investigators. A 47mm polycarbonate membrane was utilized at each sampling site. In addition, the metagenomic approach using the new generation 454 high-throughput DNA sequencing system was employed to compensate for the small sample size. Two sample sets were collected in summer 2010 and fall 2011 from the sea surface microlayer and underlying water (20 cm deep). A contamination assessment was carried out to determine that contamination might have been caused during the use of the sampling techniques. A total of 14,120 bacterial 16S rRNA gene sequences with an average length of 437.8 bp were obtained. A total of 1,254 Operational Taxonomic Units (OTUs) were constructed and 268 genera were identified. The results indicated that the bacterial compositions of the sea surface microlayer samples were distinct from those of the underlying water samples. This experiment demonstrated that the new generation sequencing platform and microbial metagenomics analysis software together served as powerful tools to gain a deeper understanding of microbial communities within the sea surface microlayer. Furthermore, it is suggested that the newly employed sampling methods could be used to obtain a snapshot of bacterial community structure as well as environmental conditions. Chapter 3 Synthetic aperture radar (SAR) remote sensing captures various fine-scale features on the ocean surface such as coastal discharge, oil pollution, vessel traffic, algal blooms and sea slicks. Although numerous factors potentially affect the SAR imaging process, the influence of biogenic and anthropogenic surfactants has been suggested as one of the primary parameters, especially under relatively low wind conditions. Surfactants have a tendency to dampen the short gravity-capillary ocean waves causing the sea surface to smoothen, thus allowing the radar to detect areas of surfactants. Surfactants are found in sea slicks, which are the accumulation of organic material shaped as elongated bands on the ocean’s surface. Sea slicks are often observable with the naked eye due to their glassy appearance and can also be seen on SAR images as dark scars. While the sources of surfactants can vary, some are known to be associated with marine bacteria. Countless numbers of marine bacteria are present in the oceanic environment, and their biogeochemical contributions cannot be overlooked. Not only do marine bacteria produce surfactants, but they also play an important role in the transformation of surfactants. In this study, we profiled the surfactant-associated bacteria composition within the biogenic thin layer of the ocean surface more commonly referred as the sea surface microlayer (SML). Bacterial samples were collected from the SML for comparative analysis from both within and outside of sea slick areas as well as the respective underlying subsurface water. The bacterial microlayer sampling coincided with SAR satellite, RADARSAT-2, overpasses to demonstrate the simultaneous in-situ measurements during a satellite image capture. The SML sampling method was designed to enable aseptic bacterial sampling. A 47 mm polycarbonate membrane was utilized at each sampling site to obtain a snapshot of the bacterial community structure at a specific space and time. Also, a new generation high-throughput sequencing method was employed to compensate for the small sample size acquired. A total of 27,006 nucleotide sequences (16S rRNA genes) with an average 437.8 bp in length were analyzed. The results revealed the presence of industrially important surfactant-producing marine bacteria, Acinetobacter, Bacillus, Corynebacterium and surfactant-degrading marine bacteria, Escherichia. In addition, Pseudomonas was detected which can be either a producer, decomposer or both. Recognizing that there is still a large number of marine bacterial species that have not been taxonomically classified nor recognized as surfactant-associated species, the effects on SAR imaging due to a high number of surfactant-associated marine bacteria is expected. This study has provided the basis for the biological importance for fine-scale synthetic aperture satellite imaging. Moreover, this new approach is expected to have applications in monitoring biological and chemical properties of the sea surface across the globe.

Le bassin profond du Bas-Congo est une marge passive affectée par une forte déformation salifère. Elle présente des sorties naturelles d'hydrocarbures lourds qui ont des impacts majeurs sociétaux, biologique, écologique et économique. L'objectif de ce travail est de comprendre les modes de fonctionnement de ces systèmes, depuis la mobilisation des hydrocarbures dans les sédiments, jusqu'à l'exutoire en fond de mer, puis en surface mer. Cette étude combine des analyses de données issues de l’imagerie spatiale, de l’océanographie, de la géochimie et de la géophysique marine afin d'obtenir à une vision intégrée multi-échelle des zones d’expulsion naturelles d'hydrocarbures dans le bassin profond du Bas-Congo. L'analyse de données issues de l'imagerie spatiale montre qu'une centaine de sites sont actifs dans la zone d'étude. Un volume de 4400 m3 d’hydrocarbures est naturellement émis par an. L'intégration des données de courantométrie permet de relier les nappes d'hydrocarbures visibles à la surface de la mer avec les structures fond de mer. Celles-ci correspondent à des chapelets de dépressions localisés sur le pourtour des diapirs de sel et à des regroupements de monticules composés d'asphaltes en périphérie. Dans les sédiments, les anomalies géophysiques caractéristiques correspondent à des cheminées verticales, délimitées par le bas par le réflecteur sismique associé à la base de stabilité des hydrates de gaz. La correspondance spatiale de ces différents critères a permis d'inventorier les sites potentiellement actifs sur les données géophysiques. Seulement 40% de ces sites sont associés à des nappes d'hydrocarbures récurrentes à la surface de la mer. / The Lower Congo Basin is a passive margin, affected by strong salt tectonics. Natural escapes of heavy hydrocarbons observed in the area have major impacts on the society, ecology, biology, and the economy. The aim of this work is to understand the mechanisms of these systems, from the mobilisation in the sediments towards the seafloor outlets and subsequently towards the sea surface. This study combines data analysis from spatial imagery, oceanography, geochemistry and marine geophysics in order to get a multi-scale integrated vision of the natural seepage situation in the Lower Congo Basin. The analysis of spatial imagery data shows that in the study area, the hundred active seeps sites expel a hydrocarbons volume of 4400 m3 per year, following an intermittent mechanism with miscellaneous frequencies from one site to another. We connected visible hydrocarbon slicks at the sea surface with seabed structures by integrating current measurements across the water column. Seafloor structures correspond to clustered pockmarks of high seismic amplitude located at the rim of salt diapirs and to clusters of mounds composed of highly degraded oil outwards. In sediments, geophysical anomalies form vertical chimneys, delimited by the seismic reflector associated with the base of gas hydrates stability downwards. The spatial correspondence of geophysical criteria enabled to inventory the potentially active sites on the geophysical data. Only 40% of these sites are associated with recurring oil slicks at the sea surface.

The sea-surface microlayer (SML) is the boundary layer at the air-sea interface where many biogeochemical processes occur. Many organisms (e.g., bacteria) produce surface active agents (surfactants) for life processes, which accumulate in the SML and dampen short gravity-capillary waves, resulting in sea surface slicks. Synthetic aperture radar (SAR) is capable of remotely sensing these features on the sea surface by measuring reflected backscatter from the ocean surface in microwaves. This study coordinates SAR overpasses with in situ SML and subsurface (SSW) microbial sample collection to guide subsequent analysis after 16s rRNA sequencing on the Illumina MiSeq. In April 2017, 138 SML and SSW samples were collected near a targeted oil-seep where the Taylor Platform was knocked down in the Gulf of Mexico, both in and out of visually-observed oil slicks. In July and August 2018, 220 SML and SSW samples were collected near the Looe Key coral reef and a coastal seagrass area. Analysis of microbial abundance and diversity between the two experiments shows that within oil slicks, surfactant- and oil-associated bacteria prefer to reside within the SSW rather than in the SML. In natural slicks in the coastal seagrass area, these bacteria are more abundant in the SML. Outside of these slicks, surfactant-associated bacteria are more abundant within the SML than the SSW. This suggests that the presence of oil reduces the habitability of the SML, whereas natural slicks created by foam and other surfactants creates a more habitable environment in the SML. With lower wind speed, abundance of these bacteria are greater, as increased wind speed results in a harsher environment. The diurnal cycle had an effect on the relative abundance of surfactant-associated bacteria in the SML and SSW. Our results demonstrate the usefulness of synthetic aperture radar to remotely sense sea surface slicks in coordination with in situ surfactant-associated bacteria data collection of the sea surface slicks.