This project was specifically aimed at using increased acceleration separation, as a method to optimize the recovery of gold in an ore body mainly consisting of hematite. The specific gravity of gold is much higher in comparison to the carrying material, making it possible to separate gold from other materials such as silica, base metals and their oxides, usually associated with gravitation–gold–recovery processes. The ore body investigated in this project originated from a mined gold reef containing a large proportion of gold locked inside the gold pyrite complexes. In the mine's processing plant a gold pyrite concentrate was produced by means of a flotation process. The roasting process that followed, oxidized the pyrite to iron oxide (hematite) and sulphur dioxide. The gold particles which were locked up inside the pyrite gold complex were exposed or liberated, allowing the chemicals to penetrate the complex and dissolve the gold. After the cyanide gold extraction process, the material was pumped on to a mine reserve dump, referred to as tailings or tailings reserve dump. The tailings usually contain iron oxides, free gold, gold associated with iron oxides and gold associated with silica, and free silica, commonly referred to as calcine. The gold content on the calcine dump was significantly lower than the flotation concentrate before the extraction of the gold and it was no longer viable for the mine to process the tailings further. As the volume of the mine reserve dump increased over the years, it became viable to recover the gold in a high volume low grade plant. Several attempts were made to recover the gold in this dump, but due to the high cost of processing and milling the material, it was not done. The norm in the mining industry is that it is impossible to concentrate the gold by means of gravity separation techniques where the average particle sizes are smaller than 50 um in diameter and upgrading with inexpensive gravity separation techniques was ruled out by the mine, because the average particle sizes were too small. The dump investigated in this project differed from other reserve dumps in that the main phase of material in this dump was hematite and not silica. A suspension of this material would have different fall–out properties than other mine reserve dumps, because of the hematite's high specific gravity compared to silica. This property of the material birthed the idea that the material will respond positively to high acceleration separation, although the particle sizes were too small for effective upgrading according to the norm in the mining industry. Using acceleration concentration as a first stage in the gold recovery process the production cost per gram of gold produced could be reduced significantly. Firstly, the volume of concentrated material to be treated in the chemical extraction process was reduced ninety percent and secondly, the gold concentration was increased significantly. If the gold could be concentrated to more than twenty grams of gold per ton, it could be extracted economically with an aggressive chemical processes. This was not possible with low grade material contained in the dump. The theoretical principle, on which this project was based, was to make use of high acceleration separation to establish separation between the particles associated with the gold, and the particles not associated with gold. Applying a high gravitational force would have an influence on the velocity by which the particles would fall–out in a suspension. As the acceleration force increased the fall–out velocity would also be increased and the particles with higher specific gravity would be affected more. A factor that was equally important was the particle size and weight distribution. A large hematite particle would compete with a small gold particle due to the similarity in weight. This could cause loss in small gold particles or retention of hematite particles with no gold content. Very little scientific information was available on the material investigated and in order to assemble a concentration plant setup, the head grade and particle size distribution for both the dump and bulk sample were determined accurately. Thereafter, chemical analyses and mineralogical examination were done on a representative sample of the bulk sample, determining the chemical composition of the material. The results obtained thereof were evaluated and used to configure a pilot plant. A large bulk sample was processed in the pilot plant and from the analytical results the efficiency could be evaluated. The results at optimum acceleration forces applied, resulted in a recovery of 5% of the mass, with a gold concentrate of 90 g/t Au, which represented 58% recovery of the gold. The hematite with high specific gravity as main phase positively influenced the high acceleration separation process. It proved that if the specific gravity of particles in a suspension were increased, high acceleration separation could be applied effectively to separate much smaller particle sizes. / Thesis (M.Sc. Engineering Sciences (Chemical and Minerals Engineering))--North-West University, Potchefstroom Campus, 2012.

This project was specifically aimed at using increased acceleration separation, as a method to optimize the recovery of gold in an ore body mainly consisting of hematite. The specific gravity of gold is much higher in comparison to the carrying material, making it possible to separate gold from other materials such as silica, base metals and their oxides, usually associated with gravitation–gold–recovery processes. The ore body investigated in this project originated from a mined gold reef containing a large proportion of gold locked inside the gold pyrite complexes. In the mine's processing plant a gold pyrite concentrate was produced by means of a flotation process. The roasting process that followed, oxidized the pyrite to iron oxide (hematite) and sulphur dioxide. The gold particles which were locked up inside the pyrite gold complex were exposed or liberated, allowing the chemicals to penetrate the complex and dissolve the gold. After the cyanide gold extraction process, the material was pumped on to a mine reserve dump, referred to as tailings or tailings reserve dump. The tailings usually contain iron oxides, free gold, gold associated with iron oxides and gold associated with silica, and free silica, commonly referred to as calcine. The gold content on the calcine dump was significantly lower than the flotation concentrate before the extraction of the gold and it was no longer viable for the mine to process the tailings further. As the volume of the mine reserve dump increased over the years, it became viable to recover the gold in a high volume low grade plant. Several attempts were made to recover the gold in this dump, but due to the high cost of processing and milling the material, it was not done. The norm in the mining industry is that it is impossible to concentrate the gold by means of gravity separation techniques where the average particle sizes are smaller than 50 um in diameter and upgrading with inexpensive gravity separation techniques was ruled out by the mine, because the average particle sizes were too small. The dump investigated in this project differed from other reserve dumps in that the main phase of material in this dump was hematite and not silica. A suspension of this material would have different fall–out properties than other mine reserve dumps, because of the hematite's high specific gravity compared to silica. This property of the material birthed the idea that the material will respond positively to high acceleration separation, although the particle sizes were too small for effective upgrading according to the norm in the mining industry. Using acceleration concentration as a first stage in the gold recovery process the production cost per gram of gold produced could be reduced significantly. Firstly, the volume of concentrated material to be treated in the chemical extraction process was reduced ninety percent and secondly, the gold concentration was increased significantly. If the gold could be concentrated to more than twenty grams of gold per ton, it could be extracted economically with an aggressive chemical processes. This was not possible with low grade material contained in the dump. The theoretical principle, on which this project was based, was to make use of high acceleration separation to establish separation between the particles associated with the gold, and the particles not associated with gold. Applying a high gravitational force would have an influence on the velocity by which the particles would fall–out in a suspension. As the acceleration force increased the fall–out velocity would also be increased and the particles with higher specific gravity would be affected more. A factor that was equally important was the particle size and weight distribution. A large hematite particle would compete with a small gold particle due to the similarity in weight. This could cause loss in small gold particles or retention of hematite particles with no gold content. Very little scientific information was available on the material investigated and in order to assemble a concentration plant setup, the head grade and particle size distribution for both the dump and bulk sample were determined accurately. Thereafter, chemical analyses and mineralogical examination were done on a representative sample of the bulk sample, determining the chemical composition of the material. The results obtained thereof were evaluated and used to configure a pilot plant. A large bulk sample was processed in the pilot plant and from the analytical results the efficiency could be evaluated. The results at optimum acceleration forces applied, resulted in a recovery of 5% of the mass, with a gold concentrate of 90 g/t Au, which represented 58% recovery of the gold. The hematite with high specific gravity as main phase positively influenced the high acceleration separation process. It proved that if the specific gravity of particles in a suspension were increased, high acceleration separation could be applied effectively to separate much smaller particle sizes. / Thesis (M.Sc. Engineering Sciences (Chemical and Minerals Engineering))--North-West University, Potchefstroom Campus, 2012.

When scattering intensity is plotted against the dimensionless quantity qR, where q is the magnitude of the scattering wave vector and R is the radius of the particle, in log-log scale the scattering curve shows a power-law structure which defines characteristic crossovers. This work reveals some new relationships between the power-law structure and the particle properties. In this work, computer simulation results from T-matrix, Mie theory, and discrete dipole approximation methods are used to study the far field intensity and the internal field of the particles. Scattering by both weakly and strongly refractive particles are studied. For weakly refractive randomly oriented spheroidal particles, how the phasor cancellation-based tip volume method can be applied to predict the scattering envelope is demonstrated. The relationship between backscattering enhancement and the curvature of the weakly refractive particles is explained. In strongly-refractive particles when the phase shift parameter is high, regions with higher field amplitudes start to appear. These regions are recognized as the hot spot regions. In this work, a proper definition is given to the hot spot region. The relationships between the hot spot region and the power-law structure, between the hot spot region and the particle morphology, and between the power-law structure and the particle morphology are extensively studied for scattering by spherical particles. A new semi-quantitative phasor analysis method is introduced, and the new method is used with color-coded phasor plots to identify how different regions of the particle contribute to the scattering pattern to get an insight into the physics behind the scattering. How different regions of the particle contribute to the second crossover (SC) and the backscattering enhancement is presented. Relationships between the SC, particle size, and relative refractive index of the particle are derived. It was identified that the scattering angle at the SC depends only on the relative refractive index of the particle. How the findings of this work can be applied to solve the inverse electromagnetic scattering problem for a single non-absorbing spherical particle is also discussed.

The innate immune protein surfactant protein (SP-) D is a carbohydrate binding protein that was originally isolated from mucosal lung tissues. Recently, studies show that SP-D binds to antibodies, including immunoglobulin M (IgM), which interacts with late apoptotic cells. Here we focus on the interaction between SP-D and IgM as they pertain to late apoptotic cell clearance. We hypothesized that the three-way interaction between IgM, SP-D and late apoptotic cells is functionally applicable to clearing late apoptotic cells from the lungs, thereby reducing lung inflammation. We show that SP-D binds to IgM and that IgM binds to the late apoptotic subclass of dying cells. We demonstrate that IgM and SP-D can both bind to late apoptotic cells in mutually distinct regions while also displaying some regional overlap. We show evidence that during LPS-induced lung inflammation both IgM and SP-D levels are elevated and this corresponds to an augmentation of apoptotic cell clearance. We illustrate that the protein interaction of IgM and SP-D is functionally relevant to apoptotic cell clearance in the lungs by showing that late apoptotic cells coated in IgM and/or SP-D are cleared more efficiently than control cells, by alveolar macrophages in vivo. Our ex vivo studies further show that these cells internalize apoptotic cells by engulfing very small particles released from the dying cells. We then showed that IgM preferentially directs the engulfment of small particles (~1 μm) by macrophages, in an apparent size-specific antibody-dependent particle clearance function. Our data reveals a novel relationship amongst IgM, SP-D, apoptotic cells, and alveolar macrophages that contributes to our understanding of apoptotic cell clearance, which may be used in the future to generate strategies addressing apoptotic cell accumulation or clearance deficiency in disease.

The innate immune protein surfactant protein (SP-) D is a carbohydrate binding protein that was originally isolated from mucosal lung tissues. Recently, studies show that SP-D binds to antibodies, including immunoglobulin M (IgM), which interacts with late apoptotic cells. Here we focus on the interaction between SP-D and IgM as they pertain to late apoptotic cell clearance. We hypothesized that the three-way interaction between IgM, SP-D and late apoptotic cells is functionally applicable to clearing late apoptotic cells from the lungs, thereby reducing lung inflammation. We show that SP-D binds to IgM and that IgM binds to the late apoptotic subclass of dying cells. We demonstrate that IgM and SP-D can both bind to late apoptotic cells in mutually distinct regions while also displaying some regional overlap. We show evidence that during LPS-induced lung inflammation both IgM and SP-D levels are elevated and this corresponds to an augmentation of apoptotic cell clearance. We illustrate that the protein interaction of IgM and SP-D is functionally relevant to apoptotic cell clearance in the lungs by showing that late apoptotic cells coated in IgM and/or SP-D are cleared more efficiently than control cells, by alveolar macrophages in vivo. Our ex vivo studies further show that these cells internalize apoptotic cells by engulfing very small particles released from the dying cells. We then showed that IgM preferentially directs the engulfment of small particles (~1 μm) by macrophages, in an apparent size-specific antibody-dependent particle clearance function. Our data reveals a novel relationship amongst IgM, SP-D, apoptotic cells, and alveolar macrophages that contributes to our understanding of apoptotic cell clearance, which may be used in the future to generate strategies addressing apoptotic cell accumulation or clearance deficiency in disease.