Although China has a huge cyclist population, the cycling condition in large cities is undesirable. The problem is mainly caused by the mixed-flow of cyclists and motorist on the road. Separation of cyclists and motorist is the key to solve the problem. Based on the research of the successful examples in Europe and a PEBOSCA inventory analysis of the cycling traffic in Nanjing, a set of suggestion is proposed on how to plan the bicycle network. A bicycle network separated from motor traffic and the planning on a cycling district division is introduced. The proposal also involves the suggestion on combination between cycling and public transport, and the experience route of historical culture and the natural beauty of the landscape.