The global food supply is being stressed by climate change, a growing population, and harmful diseases. One risk to vital cereal crops such as wheat and barley is Fusarium head blight (FHB), caused by the fungal plant pathogen Fusarium graminearum. Ascospores of the fungus are released from perithecia on the residues of corn and small grains and can be transported long distances (>500 m) through the atmosphere. The overall objective of this work was to assess the influence of meteorological conditions and perithecial maturity on ascospore release. The research focuses on F. graminearum because of its damaging impact to staple crops and the global ubiquity of FHB. The first specific objective was to apply state-of-the-science techniques to identify causal meteorological variables of ascospore release. We analyzed field measurements of airborne ascospores against meteorological conditions at Virginia Tech's Kentland Farm, Blacksburg, Virginia, USA and used convergent cross mapping and multivariate state space reconstruction to identify significant causal agents within this complicated natural and dynamic system. We identified relative humidity, solar radiation, wind speed, and air temperature as predictors of ascospore release. Our second research objective was to understand the impact of varying meteorological conditions on ascospore release under controlled environmental conditions. We assessed ascospore release in a chamber with controlled temperature (15°C and 25°C) and relative humidity (60%, 75%, and 95%). Ascospores released from ascospore-producing structures (perithecia) were captured on microscope slides placed inside of 3D-printed ascospore discharge devices. Results showed the sensitivity of ascospore release to relative humidity and temperature, with cool temperature and high relative humidity resulting in greater quantities of ascospores released. Our third research objective was to determine the relationship between the maturity, the number of ascospores, and the hardness of perithecia. A mechanical compression testing instrument was used to investigate the hardness of perithecia at various stages of maturity, producing a mean perithecium compression constant quantifying the uniaxial compression force required to rupture a perithecium. Results indicated that old perithecia contain the greatest amount of ascospores and exhibit increased resiliency, requiring greater forces to rupture, compared to young perithecia. This research has illustrated the complexities of F. graminearum ascospore release by describing the impact of several meteorological conditions and perithecial maturity on the timing and quantity of released ascospores. Collectively, our results may inform wheat growers on the nature and timing of ascospore release, which could help inform FHB management decisions in the future. / Ph. D.

Dispersal, especially long-distance dispersal, is an important component in many disciplines within biology. Many species are passively dispersed by wind, not least spore-dispersed organisms. In this thesis I investigated the dispersal capacity of bryophytes by studying the colonization patterns from local scales (100 m) to landscape scales (20 km). The dispersal distances were measured from a known source (up to 600 m away) or inferred from a connectivity measure (1–20 km). I introduced acidic clay to measure the colonization rates over one season of a pioneer moss, Discelium nudum (I–III). I also investigated which vascular plants and bryophytes that had colonized limed mires approximately 20–30 years after the first disturbance (IV). Discelium effectively colonized new disturbed substrates over one season. Most spores were deposited up to 50 meters from a source but the relationship between local colonization rates and connectivity increased with distance up to 20 km (I–III). Also calcicolous wetland bryophyte species were good colonizers over similar distances, while vascular plants in the same environment colonized less frequently. Common bryophytes that produce spores frequently were more effective colonizers, while no effect of spore size was detected (IV). A mechanistic model that take into account meteorological parameters to simulate the trajectories for spores of Discelium nudum fitted rather well to the observed colonization pattern, especially if spore release thresholds in wind variation and humidity were accounted for (III). This thesis conclude that bryophytes in open habitats can disperse effectively across landscapes given that the regional spore source is large enough (i.e. are common in the region and produce spores abundantly). For spore-dispersed organisms in open landscapes I suggest that it is often the colonization phase and not the transport that is the main bottle-neck for maintaining populations across landscapes. / <p>At the time of the doctoral defence the following papesr were unpublished and had  a status as follows: Paper 2: Epubl ahead of print; Paper 3: Manuscript; Paper 4: Manuscript</p>

Concern regarding the health effects of indoor air quality has grown in recent years, due to the increased prevalence of many diseases, as well as the fact that many people now spend most of their time indoors. While numerous studies have reported on the dynamics of aerosols indoors, the dynamics of bioaerosols in indoor environments are still poorly understood and very few studies have focused on fungal spore dynamics in indoor environments. Consequently, this work investigated the dynamics of fungal spores in indoor air, including fungal spore release and deposition, as well as investigating the mechanisms involved in the fungal spore fragmentation process. In relation to the investigation of fungal spore dynamics, it was found that the deposition rates of the bioaerosols (fungal propagules) were in the same range as the deposition rates of nonbiological particles and that they were a function of their aerodynamic diameters. It was also found that fungal particle deposition rates increased with increasing ventilation rates. These results (which are reported for the first time) are important for developing an understanding of the dynamics of fungal spores in the air. In relation to the process of fungal spore fragmentation, important information was generated concerning the airborne dynamics of the spores, as well as the part/s of the fungi which undergo fragmentation. The results obtained from these investigations into the dynamics of fungal propagules in indoor air significantly advance knowledge about the fate of fungal propagules in indoor air, as well as their deposition in the respiratory tract. The need to develop an advanced, real-time method for monitoring bioaerosols has become increasingly important in recent years, particularly as a result of the increased threat from biological weapons and bioterrorism. However, to date, the Ultraviolet Aerodynamic Particle Sizer (UVAPS, Model 3312, TSI, St Paul, MN) is the only commercially available instrument capable of monitoring and measuring viable airborne micro-organisms in real-time. Therefore (for the first time), this work also investigated the ability of the UVAPS to measure and characterise fungal spores in indoor air. The UVAPS was found to be sufficiently sensitive for detecting and measuring fungal propagules. Based on fungal spore size distributions, together with fluorescent percentages and intensities, it was also found to be capable of discriminating between two fungal spore species, under controlled laboratory conditions. In the field, however, it would not be possible to use the UVAPS to differentiate between different fungal spore species because the different micro-organisms present in the air may not only vary in age, but may have also been subjected to different environmental conditions. In addition, while the real-time UVAPS was found to be a good tool for the investigation of fungal particles under controlled conditions, it was not found to be selective for bioaerosols only (as per design specifications). In conclusion, the UVAPS is not recommended for use in the direct measurement of airborne viable bioaerosols in the field, including fungal particles, and further investigations into the nature of the micro-organisms, the UVAPS itself and/or its use in conjunction with other conventional biosamplers, are necessary in order to obtain more realistic results. Overall, the results obtained from this work on airborne fungal particle dynamics will contribute towards improving the detection capabilities of the UVAPS, so that it is capable of selectively monitoring and measuring bioaerosols, for which it was originally designed. This work will assist in finding and/or improving other technologies capable of the real-time monitoring of bioaerosols. The knowledge obtained from this work will also be of benefit in various other bioaerosol applications, such as understanding the transport of bioaerosols indoors.