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Seasonal comparison on the effectiveness of control methods for Microstegium vimineum in the North Carolina PiedmontBeam, Casey 09 December 2022 (has links)
Microstegium vimineum, or Japanese stilt grass, is an invasive species that readily outcompetes native vegetation and is of poor forage quality for wildlife. This species is widespread throughout the southeastern United States, including North Carolina. Much of this region is privately owned and there is a gap in the literature providing succinct information about the best methods of treatment, timing of treatment, and effects of treatment on native herbaceous plants in this region. In two parts, this study seeks to address this gap by employing treatments that are easily accessible to landowners at different times during the growing season and assessing the effects on the herbaceous plant community post-treatment. Results of this study found that application of glyphosate was the most effective treatment at M. vimineum removal, application of household vinegar was the most effective at increasing post-treatment species richness and diversity, and that seasonality did not play a role in the effectiveness of treatment options. This study will increase landowner knowledge on treating this invasive successfully and choosing a treatment that fosters an understory that meets their management goals.
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Fluxes of Atmospheric Methane Using Novel Instruments, Field Measurements, and Inverse ModelingSantoni, Gregory Winn 25 September 2013 (has links)
The atmospheric concentration of methane \((CH_4)\) - the most significant non-\(CO_2\) anthropogenic long-lived greenhouse gas - stabilized between 1999 and 2006 and then began to rise again. Explanations for this behavior differ but studies agree that more measurements and better modeling are needed to reliably explain the model-data discrepancies and predict future change. This dissertation focuses on measurements of \(CH_4\) and inverse modeling of atmospheric \(CH_4\) fluxes using field measurements at a variety of spatial scales. We first present a new fast-response instrument to measure the isotopic composition of \(CH_4\) in ambient air. The instrument was used to characterize mass fluxes and isofluxes (a isotopically-weighted mass flux) from a well-studied research fen in New Hampshire. Eddy-covariance and automatic chamber techniques produced consistent estimates of both the \(CH_4\) fluxes and their isotopic composition at sub-hourly resolution. We then characterize fluxes of \(CH_4\) from aircraft engines using measurements made with the same instrument during the Alternative Aviation Fuel Experiment (AAFEX), a study that aimed to determine the atmospheric impacts of alternative fuel use in the growing aviation industry. Emissions of \(CO_2\), \(CH_4\), and \(N_2O\) from different synthetic fuels were statistically indistinguishable from those of the widely used JP-8 jet fuel. We then present airborne observations of the long-lived greenhouse gas suite – \(CO_2\), \(CH_4\), \(N_2O\), and CO – during two aircraft campaigns, HIPPO and CalNex, made using a similar instrument built specifically for the NCAR HIAPER GV aircraft. These measurements are compared to data from other onboard sensors and show excellent agreement. We discuss the details of the end-to-end calibration procedures and the data quality-assurance and quality-control (QA/QC). Lastly, we quantify a top-down estimate of California’s \(CH_4\) emission inventory using the CalNex \(CH_4\) observations. Observed \(CH_4\) enhancements above background concentrations are simulated using a lagrangian transport model driven by validated meteorology. A priori source-specific emission inventories are optimized in a Bayesian inversion framework to show that California’s \(CH_4\) budget is 1.6 ± 0.34 times larger than the current estimate of California’s Air Resources Board (CARB), the body charged with enforcing the California Global Solutions Act and tracking emission changes over time. Findings highlight large underestimates of emissions from cattle and natural gas infrastructure. / Earth and Planetary Sciences
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The Foods and Feeding Habits of Botulism Intoxicated and Healthy Waterbirds on the Bear River Refuge, Utah, with Emphasis on the American Avocet and Blacknecked StiltWilson, George W. 01 May 1973 (has links)
Primary objectives of this study were: (1) to compare the food habits of healthy and botulism intoxicated American avocets and blacknecked stilts for qualitative and quantitative differences, (2) to determine .which invertebrates found in the tracts of intoxicated and healthy birds contained type C toxin, and (3) to explain apparent susceptibilities among different sexes, ages and species of birds.
Based on percent utilization and occurrence of food items in the esophagus , the diets of healthy and intoxicated birds were similar. However, intoxicated avocets consumed higher proportions of Gastropoda and intoxicated stilts more Hydrophilidae larvae than did healthy birds. Because of similarities in diets of healthy and intoxicated birds, birds probably became intoxicated from eating randomly toxic foods and not because of differences in diet or food preference.
Examinations of esophageal contents of avocets and stilts better described recently consumed food items than did examinations of gizzard contents. Soft-bodied invertebrates were rendered unidentifiable in the gizzard. The esophageal contents of juvenile avocets and stilts contained greater proportions of animal matter than did those of adults.
Only three of 86 food samples analyzed were definitely positive for type C botulism toxin. These were: one sample of Tendipedidae larvae and two of pooled gizzard contents which included fragments of Corixidae, Hydrophilidae adults, seeds and vegetative fibers.
Only three of 86 food samples analyzed were definitely positive for type C botulism toxin. These were: one sample of Tendipedidae larvae and two of pooled gizzard contents which included fragments of Corixidae, Hydrophilidae adults, seeds and vegetative fibers.
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Modeling flood-induced processes causing Russell lupin mortality in the braided Ahuriri River, New ZealandJavernick, Luke Anthony January 2013 (has links)
The braided rivers and floodplains in the Upper Waitaki Basin (UWB) of the South Island of New Zealand are critical habitats for endangered and threatened fauna such as the black stilt. However, this habitat has degraded due to introduced predators, hydropower operations, and invasive weeds including Russell lupins. While conservation efforts have been made to restore these habitats, flood events may provide a natural mechanism for removal of invasive vegetation and re-creation of natural floodplain habitats. However, little is understood about the hydraulic effects of floods on vegetation and potential mortality in these dynamic systems. Therefore, this thesis analyzed the flood-induced processes that cause lupin mortality in a reach of the Ahuriri River in the UWB, and simulated various sized flood events to assess how and where these processes occurred.
To determine the processes that cause lupin mortality, post-flood observations were utilized to develop the hypothesis that flood-induced drag, erosion, sediment deposition, inundation, and trauma were responsible. Field and laboratory experiments were conducted to evaluate and quantify these individual processes, and results showed that drag, erosion, sediment deposition and inundation could cause lupin mortality. Utilizing these mortality processes, mortality thresholds of velocity, water depth, inundation duration, and morphologic changes were estimated through data analysis and evaluation of various empirical relationships.
Delft3D was the numerical model used to simulate 2-dimensional flood hydraulics in the study-reach and was calibrated in three stages for hydraulics, vegetation, and morphology. Hydraulic calibration was achieved using the study-reach topography captured by Structure-from-Motion (SfM) and various hydraulic data (depth, velocity, and water extent from aerial photographs). Vegetation inclusion in Delft3D was possible utilizing a function called ‘trachytopes’, which represented vegetation roughness and flow resistance and was calibrated utilizing data from a lupin-altered flow conveyance experiment. Morphologic calibration was achieved by simulating an observed near-mean annual flood event (209 m3 s-1) and adjusting the model parameters until the simulated morphologic changes best represented the observed morphologic changes captured by pre- and post-flood SfM digital elevation models. Calibration results showed that hydraulics were well represented, vegetation inclusion often improved the simulated water inundation extent accuracy at high flows, but that local erosion and sediment deposition were difficult to replicate. Simulation of morphological change was expected to be limited due to simplistic bank erosion prediction methods. Nevertheless, the model was considered adequate since simulated total bank erosion was comparable to that observed and realistic river characteristics (riffles, pools, and channel width) were produced.
Flood events ranging from the 2- to 500-year flood were simulated with the calibrated model, and lupin mortality was estimated using simulation results with the lupin mortality thresholds. Results showed that various degrees of lupin mortality occurred for the different flood events, but that the dominant mortality processes fluctuated between erosion, drag, and inundation. Sediment deposition-induced mortality was minimal, but was likely under-represented in the modeling due to poor model sediment deposition replication and possibly over-restrictive deposition mortality thresholds. The research presented in this thesis provided greater understanding of how natural flood events restore and preserve the floodplain habitats of the UWB and can be used to aid current and future braided river conservation and restoration efforts.
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Anthelmintic treatment and digestive organ morphology of captive-reared kaki (Himantopus novaezelandiae) released to the wild : a thesis presented in partial fulfilment of the requirements for the degree of Master of Science in Conservation Biology at Massey University, Palmerston North, New ZealandRobertson, Louise Mary January 2009 (has links)
The continued existence of New Zealand’s critically endangered and endemic black stilt or kakï (Himantopus novaezelandiae) relies on an intensive captive management programme. While this is successful at rearing large numbers of birds for release to the wild, poor survivability of these birds is limiting significant increases in the wild population. Predation and starvation are suspected to be the most common causes of death in released birds, but underlying contributing factors to these mortalities have not been fully evaluated. This research investigates the possible contribution of gastrointestinal (GI) helminth burdens and suboptimal digestive organ development to the high mortality rates of released kakï. Emphasis is placed on evaluating the methods used to assess the importance of these factors and to make informed recommendations for future management. The efficacy of the anthelmintic regime used for kakï was assessed by dosing half of the 80 captive birds with praziquantel (PZQ) prior to release in 2007. Faecal samples were collected before and after anthelmintic treatment, and before and after release to the wild. Post mortem worm counts were conducted on 11 birds that died following release and historical worm count records dating back to 1997 were accessed. The main findings were: PZQ had high efficacy against trematodes; treatment did not prevent re-infection; birds were exposed to helminths at release site; and there was no advantage of treatment for survival. Overall, the results suggest that anthelmintic treatment is an unnecessary cost. Consequently, recommendations were made to cease anthelmintic treatment or reduce its intensity, continue health screening, and incorporate annual efficacy testing to monitor the emergence of anthelmintic resistance. The reliability of faecal screening for GI helminths was evaluated. Faecal egg counts (FECs) were found to be poor indicators of worm burden. The two modified sedimentation methods used to detect trematodes provided relatively low egg recovery rates. Trematode egg shedding varied between days but not by hour of the day or temperature. The collection and analysis of pooled faecal samples proved to be more cost and time-effective than samples from individual birds and the larger masses collected resulted in greater sensitivity. In conclusion, faecal analysis of pooled samples is a useful qualitative indicator of helminth presence or absence but is quantitatively unreliable. In order to assess the importance of digestive organ development to captive-reared and released kak?, the digestive organs of healthy and emaciated captive, released and wild Himantopus sp. were compared. Captive and released kak? had generally smaller digestive organs than wild birds, released birds did not increase digestive organ size to match the high fibre wild diet, and emaciated birds did not have atrophied organs. However, the greatest mortality rates occur soon after release, while the birds were still being supplementary fed. These results suggest that reduced digestive efficiency is probably not contributing significantly to mortality rates and the direct impacts of the translocation are probably greater risk factors. The continued provision of supplementary food to released birds and an increased focus on habitat enhancement and predator control at release sites were recommended. The reliability of comparing fresh and formalin fixed Himantopus sp. gut specimens was evaluated. Significant differences in fresh and formalin fixed organ dry masses and variation in preserved organ lengths indicate that this variation should be considered in future studies. In conclusion, current management practices appear to be successful in ensuring that GI helminths and reduced digestive efficiency do not significantly lower the survivorship of captive-reared and released kakï. There is a need for further research into developing a more direct physiological assessment of the impacts of GI helminths and gut morphology as well as considering the role that starvation may have on poor survivability.
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Anthelmintic treatment and digestive organ morphology of captive-reared kaki (Himantopus novaezelandiae) released to the wild : a thesis presented in partial fulfilment of the requirements for the degree of Master of Science in Conservation Biology at Massey University, Palmerston North, New ZealandRobertson, Louise Mary January 2009 (has links)
The continued existence of New Zealand’s critically endangered and endemic black stilt or kakï (Himantopus novaezelandiae) relies on an intensive captive management programme. While this is successful at rearing large numbers of birds for release to the wild, poor survivability of these birds is limiting significant increases in the wild population. Predation and starvation are suspected to be the most common causes of death in released birds, but underlying contributing factors to these mortalities have not been fully evaluated. This research investigates the possible contribution of gastrointestinal (GI) helminth burdens and suboptimal digestive organ development to the high mortality rates of released kakï. Emphasis is placed on evaluating the methods used to assess the importance of these factors and to make informed recommendations for future management. The efficacy of the anthelmintic regime used for kakï was assessed by dosing half of the 80 captive birds with praziquantel (PZQ) prior to release in 2007. Faecal samples were collected before and after anthelmintic treatment, and before and after release to the wild. Post mortem worm counts were conducted on 11 birds that died following release and historical worm count records dating back to 1997 were accessed. The main findings were: PZQ had high efficacy against trematodes; treatment did not prevent re-infection; birds were exposed to helminths at release site; and there was no advantage of treatment for survival. Overall, the results suggest that anthelmintic treatment is an unnecessary cost. Consequently, recommendations were made to cease anthelmintic treatment or reduce its intensity, continue health screening, and incorporate annual efficacy testing to monitor the emergence of anthelmintic resistance. The reliability of faecal screening for GI helminths was evaluated. Faecal egg counts (FECs) were found to be poor indicators of worm burden. The two modified sedimentation methods used to detect trematodes provided relatively low egg recovery rates. Trematode egg shedding varied between days but not by hour of the day or temperature. The collection and analysis of pooled faecal samples proved to be more cost and time-effective than samples from individual birds and the larger masses collected resulted in greater sensitivity. In conclusion, faecal analysis of pooled samples is a useful qualitative indicator of helminth presence or absence but is quantitatively unreliable. In order to assess the importance of digestive organ development to captive-reared and released kak?, the digestive organs of healthy and emaciated captive, released and wild Himantopus sp. were compared. Captive and released kak? had generally smaller digestive organs than wild birds, released birds did not increase digestive organ size to match the high fibre wild diet, and emaciated birds did not have atrophied organs. However, the greatest mortality rates occur soon after release, while the birds were still being supplementary fed. These results suggest that reduced digestive efficiency is probably not contributing significantly to mortality rates and the direct impacts of the translocation are probably greater risk factors. The continued provision of supplementary food to released birds and an increased focus on habitat enhancement and predator control at release sites were recommended. The reliability of comparing fresh and formalin fixed Himantopus sp. gut specimens was evaluated. Significant differences in fresh and formalin fixed organ dry masses and variation in preserved organ lengths indicate that this variation should be considered in future studies. In conclusion, current management practices appear to be successful in ensuring that GI helminths and reduced digestive efficiency do not significantly lower the survivorship of captive-reared and released kakï. There is a need for further research into developing a more direct physiological assessment of the impacts of GI helminths and gut morphology as well as considering the role that starvation may have on poor survivability.
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Anthelmintic treatment and digestive organ morphology of captive-reared kaki (Himantopus novaezelandiae) released to the wild : a thesis presented in partial fulfilment of the requirements for the degree of Master of Science in Conservation Biology at Massey University, Palmerston North, New ZealandRobertson, Louise Mary January 2009 (has links)
The continued existence of New Zealand’s critically endangered and endemic black stilt or kakï (Himantopus novaezelandiae) relies on an intensive captive management programme. While this is successful at rearing large numbers of birds for release to the wild, poor survivability of these birds is limiting significant increases in the wild population. Predation and starvation are suspected to be the most common causes of death in released birds, but underlying contributing factors to these mortalities have not been fully evaluated. This research investigates the possible contribution of gastrointestinal (GI) helminth burdens and suboptimal digestive organ development to the high mortality rates of released kakï. Emphasis is placed on evaluating the methods used to assess the importance of these factors and to make informed recommendations for future management. The efficacy of the anthelmintic regime used for kakï was assessed by dosing half of the 80 captive birds with praziquantel (PZQ) prior to release in 2007. Faecal samples were collected before and after anthelmintic treatment, and before and after release to the wild. Post mortem worm counts were conducted on 11 birds that died following release and historical worm count records dating back to 1997 were accessed. The main findings were: PZQ had high efficacy against trematodes; treatment did not prevent re-infection; birds were exposed to helminths at release site; and there was no advantage of treatment for survival. Overall, the results suggest that anthelmintic treatment is an unnecessary cost. Consequently, recommendations were made to cease anthelmintic treatment or reduce its intensity, continue health screening, and incorporate annual efficacy testing to monitor the emergence of anthelmintic resistance. The reliability of faecal screening for GI helminths was evaluated. Faecal egg counts (FECs) were found to be poor indicators of worm burden. The two modified sedimentation methods used to detect trematodes provided relatively low egg recovery rates. Trematode egg shedding varied between days but not by hour of the day or temperature. The collection and analysis of pooled faecal samples proved to be more cost and time-effective than samples from individual birds and the larger masses collected resulted in greater sensitivity. In conclusion, faecal analysis of pooled samples is a useful qualitative indicator of helminth presence or absence but is quantitatively unreliable. In order to assess the importance of digestive organ development to captive-reared and released kak?, the digestive organs of healthy and emaciated captive, released and wild Himantopus sp. were compared. Captive and released kak? had generally smaller digestive organs than wild birds, released birds did not increase digestive organ size to match the high fibre wild diet, and emaciated birds did not have atrophied organs. However, the greatest mortality rates occur soon after release, while the birds were still being supplementary fed. These results suggest that reduced digestive efficiency is probably not contributing significantly to mortality rates and the direct impacts of the translocation are probably greater risk factors. The continued provision of supplementary food to released birds and an increased focus on habitat enhancement and predator control at release sites were recommended. The reliability of comparing fresh and formalin fixed Himantopus sp. gut specimens was evaluated. Significant differences in fresh and formalin fixed organ dry masses and variation in preserved organ lengths indicate that this variation should be considered in future studies. In conclusion, current management practices appear to be successful in ensuring that GI helminths and reduced digestive efficiency do not significantly lower the survivorship of captive-reared and released kakï. There is a need for further research into developing a more direct physiological assessment of the impacts of GI helminths and gut morphology as well as considering the role that starvation may have on poor survivability.
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Anthelmintic treatment and digestive organ morphology of captive-reared kaki (Himantopus novaezelandiae) released to the wild : a thesis presented in partial fulfilment of the requirements for the degree of Master of Science in Conservation Biology at Massey University, Palmerston North, New ZealandRobertson, Louise Mary January 2009 (has links)
The continued existence of New Zealand’s critically endangered and endemic black stilt or kakï (Himantopus novaezelandiae) relies on an intensive captive management programme. While this is successful at rearing large numbers of birds for release to the wild, poor survivability of these birds is limiting significant increases in the wild population. Predation and starvation are suspected to be the most common causes of death in released birds, but underlying contributing factors to these mortalities have not been fully evaluated. This research investigates the possible contribution of gastrointestinal (GI) helminth burdens and suboptimal digestive organ development to the high mortality rates of released kakï. Emphasis is placed on evaluating the methods used to assess the importance of these factors and to make informed recommendations for future management. The efficacy of the anthelmintic regime used for kakï was assessed by dosing half of the 80 captive birds with praziquantel (PZQ) prior to release in 2007. Faecal samples were collected before and after anthelmintic treatment, and before and after release to the wild. Post mortem worm counts were conducted on 11 birds that died following release and historical worm count records dating back to 1997 were accessed. The main findings were: PZQ had high efficacy against trematodes; treatment did not prevent re-infection; birds were exposed to helminths at release site; and there was no advantage of treatment for survival. Overall, the results suggest that anthelmintic treatment is an unnecessary cost. Consequently, recommendations were made to cease anthelmintic treatment or reduce its intensity, continue health screening, and incorporate annual efficacy testing to monitor the emergence of anthelmintic resistance. The reliability of faecal screening for GI helminths was evaluated. Faecal egg counts (FECs) were found to be poor indicators of worm burden. The two modified sedimentation methods used to detect trematodes provided relatively low egg recovery rates. Trematode egg shedding varied between days but not by hour of the day or temperature. The collection and analysis of pooled faecal samples proved to be more cost and time-effective than samples from individual birds and the larger masses collected resulted in greater sensitivity. In conclusion, faecal analysis of pooled samples is a useful qualitative indicator of helminth presence or absence but is quantitatively unreliable. In order to assess the importance of digestive organ development to captive-reared and released kak?, the digestive organs of healthy and emaciated captive, released and wild Himantopus sp. were compared. Captive and released kak? had generally smaller digestive organs than wild birds, released birds did not increase digestive organ size to match the high fibre wild diet, and emaciated birds did not have atrophied organs. However, the greatest mortality rates occur soon after release, while the birds were still being supplementary fed. These results suggest that reduced digestive efficiency is probably not contributing significantly to mortality rates and the direct impacts of the translocation are probably greater risk factors. The continued provision of supplementary food to released birds and an increased focus on habitat enhancement and predator control at release sites were recommended. The reliability of comparing fresh and formalin fixed Himantopus sp. gut specimens was evaluated. Significant differences in fresh and formalin fixed organ dry masses and variation in preserved organ lengths indicate that this variation should be considered in future studies. In conclusion, current management practices appear to be successful in ensuring that GI helminths and reduced digestive efficiency do not significantly lower the survivorship of captive-reared and released kakï. There is a need for further research into developing a more direct physiological assessment of the impacts of GI helminths and gut morphology as well as considering the role that starvation may have on poor survivability.
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Anthelmintic treatment and digestive organ morphology of captive-reared kaki (Himantopus novaezelandiae) released to the wild : a thesis presented in partial fulfilment of the requirements for the degree of Master of Science in Conservation Biology at Massey University, Palmerston North, New ZealandRobertson, Louise Mary January 2009 (has links)
The continued existence of New Zealand’s critically endangered and endemic black stilt or kakï (Himantopus novaezelandiae) relies on an intensive captive management programme. While this is successful at rearing large numbers of birds for release to the wild, poor survivability of these birds is limiting significant increases in the wild population. Predation and starvation are suspected to be the most common causes of death in released birds, but underlying contributing factors to these mortalities have not been fully evaluated. This research investigates the possible contribution of gastrointestinal (GI) helminth burdens and suboptimal digestive organ development to the high mortality rates of released kakï. Emphasis is placed on evaluating the methods used to assess the importance of these factors and to make informed recommendations for future management. The efficacy of the anthelmintic regime used for kakï was assessed by dosing half of the 80 captive birds with praziquantel (PZQ) prior to release in 2007. Faecal samples were collected before and after anthelmintic treatment, and before and after release to the wild. Post mortem worm counts were conducted on 11 birds that died following release and historical worm count records dating back to 1997 were accessed. The main findings were: PZQ had high efficacy against trematodes; treatment did not prevent re-infection; birds were exposed to helminths at release site; and there was no advantage of treatment for survival. Overall, the results suggest that anthelmintic treatment is an unnecessary cost. Consequently, recommendations were made to cease anthelmintic treatment or reduce its intensity, continue health screening, and incorporate annual efficacy testing to monitor the emergence of anthelmintic resistance. The reliability of faecal screening for GI helminths was evaluated. Faecal egg counts (FECs) were found to be poor indicators of worm burden. The two modified sedimentation methods used to detect trematodes provided relatively low egg recovery rates. Trematode egg shedding varied between days but not by hour of the day or temperature. The collection and analysis of pooled faecal samples proved to be more cost and time-effective than samples from individual birds and the larger masses collected resulted in greater sensitivity. In conclusion, faecal analysis of pooled samples is a useful qualitative indicator of helminth presence or absence but is quantitatively unreliable. In order to assess the importance of digestive organ development to captive-reared and released kak?, the digestive organs of healthy and emaciated captive, released and wild Himantopus sp. were compared. Captive and released kak? had generally smaller digestive organs than wild birds, released birds did not increase digestive organ size to match the high fibre wild diet, and emaciated birds did not have atrophied organs. However, the greatest mortality rates occur soon after release, while the birds were still being supplementary fed. These results suggest that reduced digestive efficiency is probably not contributing significantly to mortality rates and the direct impacts of the translocation are probably greater risk factors. The continued provision of supplementary food to released birds and an increased focus on habitat enhancement and predator control at release sites were recommended. The reliability of comparing fresh and formalin fixed Himantopus sp. gut specimens was evaluated. Significant differences in fresh and formalin fixed organ dry masses and variation in preserved organ lengths indicate that this variation should be considered in future studies. In conclusion, current management practices appear to be successful in ensuring that GI helminths and reduced digestive efficiency do not significantly lower the survivorship of captive-reared and released kakï. There is a need for further research into developing a more direct physiological assessment of the impacts of GI helminths and gut morphology as well as considering the role that starvation may have on poor survivability.
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Anthelmintic treatment and digestive organ morphology of captive-reared kaki (Himantopus novaezelandiae) released to the wild : a thesis presented in partial fulfilment of the requirements for the degree of Master of Science in Conservation Biology at Massey University, Palmerston North, New ZealandRobertson, Louise Mary January 2009 (has links)
The continued existence of New Zealand’s critically endangered and endemic black stilt or kakï (Himantopus novaezelandiae) relies on an intensive captive management programme. While this is successful at rearing large numbers of birds for release to the wild, poor survivability of these birds is limiting significant increases in the wild population. Predation and starvation are suspected to be the most common causes of death in released birds, but underlying contributing factors to these mortalities have not been fully evaluated. This research investigates the possible contribution of gastrointestinal (GI) helminth burdens and suboptimal digestive organ development to the high mortality rates of released kakï. Emphasis is placed on evaluating the methods used to assess the importance of these factors and to make informed recommendations for future management. The efficacy of the anthelmintic regime used for kakï was assessed by dosing half of the 80 captive birds with praziquantel (PZQ) prior to release in 2007. Faecal samples were collected before and after anthelmintic treatment, and before and after release to the wild. Post mortem worm counts were conducted on 11 birds that died following release and historical worm count records dating back to 1997 were accessed. The main findings were: PZQ had high efficacy against trematodes; treatment did not prevent re-infection; birds were exposed to helminths at release site; and there was no advantage of treatment for survival. Overall, the results suggest that anthelmintic treatment is an unnecessary cost. Consequently, recommendations were made to cease anthelmintic treatment or reduce its intensity, continue health screening, and incorporate annual efficacy testing to monitor the emergence of anthelmintic resistance. The reliability of faecal screening for GI helminths was evaluated. Faecal egg counts (FECs) were found to be poor indicators of worm burden. The two modified sedimentation methods used to detect trematodes provided relatively low egg recovery rates. Trematode egg shedding varied between days but not by hour of the day or temperature. The collection and analysis of pooled faecal samples proved to be more cost and time-effective than samples from individual birds and the larger masses collected resulted in greater sensitivity. In conclusion, faecal analysis of pooled samples is a useful qualitative indicator of helminth presence or absence but is quantitatively unreliable. In order to assess the importance of digestive organ development to captive-reared and released kak?, the digestive organs of healthy and emaciated captive, released and wild Himantopus sp. were compared. Captive and released kak? had generally smaller digestive organs than wild birds, released birds did not increase digestive organ size to match the high fibre wild diet, and emaciated birds did not have atrophied organs. However, the greatest mortality rates occur soon after release, while the birds were still being supplementary fed. These results suggest that reduced digestive efficiency is probably not contributing significantly to mortality rates and the direct impacts of the translocation are probably greater risk factors. The continued provision of supplementary food to released birds and an increased focus on habitat enhancement and predator control at release sites were recommended. The reliability of comparing fresh and formalin fixed Himantopus sp. gut specimens was evaluated. Significant differences in fresh and formalin fixed organ dry masses and variation in preserved organ lengths indicate that this variation should be considered in future studies. In conclusion, current management practices appear to be successful in ensuring that GI helminths and reduced digestive efficiency do not significantly lower the survivorship of captive-reared and released kakï. There is a need for further research into developing a more direct physiological assessment of the impacts of GI helminths and gut morphology as well as considering the role that starvation may have on poor survivability.
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