by Chan Chi Wai, Michael. / "July 2002." / Thesis (Ph.D.)--Chinese University of Hong Kong, 2002. / Includes bibliographical references (p. 238-272). / Electronic reproduction. Hong Kong : Chinese University of Hong Kong, [2012] System requirements: Adobe Acrobat Reader. Available via World Wide Web. / Electronic reproduction. Ann Arbor, MI : ProQuest Information and Learning Company, [200-] System requirements: Adobe Acrobat Reader. Available via World Wide Web. / Mode of access: World Wide Web. / Abstracts in English and Chinese.

Lee Tin Lap. / "January 2001." / Thesis (Ph.D.)--Chinese University of Hong Kong, 2001. / Includes bibliographical references (p. 161-185). / Electronic reproduction. Hong Kong : Chinese University of Hong Kong, [2012] System requirements: Adobe Acrobat Reader. Available via World Wide Web. / Mode of access: World Wide Web. / Abstracts in English and Chinese.

Orientador: Marcia Rita Fernandes Machado / Banca: Fabrício Singaretti de Oliveira / Banca: Paola Castro Moraes / Resumo: Neste estudo, implantou-se biomembrana de látex natural em defeito (1,5 x 1,5 cm) induzido na parede de estômago de coelhos, com o intuito de se avaliar o processo de reparação tecidual no que se refere à biocompatibilidade, capacidade de reparação e possíveis complicações. Utilizaram-se 24 coelhos, albinos, raça Nova Zelândia Branco, adultos, machos inteiros, divididos em grupos experimentais: Grupo Biomembrana Fundo (GBF), Grupo Biomembrana Corpo (GBC), Grupo Controle Fundo (GCF) e Grupo Controle Corpo (GCC). Aos quinze, 30 e 60 dias de pós-operatório, os animais foram sacrificados, para as avaliações. Macroscopicamente, aos quinze dias de pós-operatório observou-se em 100% dos animais presença de aderências na face serosa e a presença da biomembrana na face luminal. Aos 30 dias de pós-operatório 37,5% dos animais avaliados apresentaram aderências na face serosa e em 62,5% dos animais não ocorreram aderências em nenhuma estrutura. Neste mesmo período, todos os animais ainda apresentavam a biomembrana na face mucosa. Aos 60 dias de pósoperatório, 50% dos animais estudados não apresentaram aderências em nenhuma estrutura e em 50% dos animais, foi observada aderências na face serosa. Neste período, em todos os animais avaliados a biomembrana não foi encontrada. Sob microscopia de luz, no grupo Biomembrana aos quinze e 30 dias verificaram-se descontinuidade das camadas muscular e mucosa, presença de infiltrado inflamatório polimorfonuclear. Foi visualizado vasos sanguíneos e fibras musculares. Aos 60 dias as camadas mucosa, muscular estavam completamente recontituídas. No grupo GC, aos quinze e 30 dias de pós-operatório verificou-se descontinuidade da camada muscular e mucosa neoformada e presença de infiltrado inflamatório mononuclear em submucosa. Já aos 60 dias, a mucosa mostrou-se reconstituída... (Resumo completo, clicar acesso eletrônico abaixo) / Abstract: In this study, biomembrane of natural latex was utilized to replace a section of face of the stomach of rabbits, with purpose of evaluation of the tissue repair process in regard of its biocompatility, scar formation ability and possible complications. Twentyfour New Zealand rabbits, adult and non-castrated males were divided in experimental groups: Biomembrane Fund Group (GBF), Biomembrane Body Group (GBC), Control Fund Group (GCF) and Control Body Group (GCC). The animals were euthanized at fifteen, 30 and 60 days of post operative, followed by analysis. Macroscopically, in fifteen days of post operative were observed in 100% of the animals presence in the serosal face, the adherences and in the luminal face the biomembrane. In 30 days of post operative 37,5% of the animals, presence in the serosal face, the adherences and 62,5% were observed in of the animals absence of the adherences. In this time, all of the animals were observed in the luminal face the biomembrane. In 60 days of post operative 50% of the animals were observed absence of the adherences, and in 50% of the animals, presence in the serosal face, the adherences. In this time the biomembrana is not in the stomach. Under light microscopy, in the Biomembrane group at fifteen and 30 days, were observed discontinuity of muscle layer and mucosa layer, presence of polimorfonuclear population of inflammatory cells. New vessels and muscle fibers were observed. In 60 days the mucosa, muscle layers were complete reconstituted. In the GP group, at 15 and 30 days of post operative were observed discontinuity of muscle layer and mucosa formed and presence of mononuclear population of inflammatory cells in submucosa. In 60 days, hypoglandular and complete layer reconstituted mucosa and muscle layers were complete reconstituted. The stereologic... (Complete abstract click electronic access below) / Mestre

Li Chung Yi. / Thesis (M.Phil.)--Chinese University of Hong Kong, 2001. / Includes bibliographical references (leaves 110-123). / Abstracts in English and Chinese. / ABSTRACT --- p.i / ACKNOWLEDGEMENTS --- p.iv / TABLE OF CONTENTS --- p.v / LIST OF FIGURES --- p.ix / LIST OF TABLES --- p.xi / Chapter I. --- INTRODUCTION --- p.1 / Chapter I.1 --- Gastric Carcinoma --- p.1 / Chapter I.2 --- Etiology of Gastric Carcinoma --- p.2 / Chapter I.2.1 --- Environmental Factors: --- p.2 / Chapter I.2.2 --- Helicobacter Pylori Infection: --- p.2 / Chapter I.2.3 --- Genetic Factors: --- p.3 / Chapter I.2.4 --- Other Factors: --- p.4 / Chapter I.3 --- The Lauren Classification of Gastric carcinoma --- p.5 / Chapter I.3.1 --- Histolopathology of Intestinal Type of Gastric Carcinoma --- p.5 / Chapter I.3.2 --- Histopathology of Diffuse Type of Gastric Carcinoma --- p.8 / Chapter I.4 --- Cytogenetics Studies in Gastric Carcinoma --- p.10 / Chapter I.4.1 --- Cytogenetic Studies of Gastric carcinoma --- p.10 / Chapter I.5 --- Molecular Studies of Gastric Carcinoma --- p.14 / Chapter I.5.1 --- Genetic Instability --- p.14 / Chapter I.5.2 --- Amplification/ Mutation of Oncogenes --- p.15 / Chapter I.5.3 --- Alterations of Tumor Suppressor Genes --- p.20 / Chapter I.5.4 --- Cell Adhesion Molecules --- p.23 / Chapter I.5.5 --- Molecular Studies on Intestinal Metaplasia --- p.27 / Chapter II. --- LONG ARM OF CHROMOSOME 6 --- p.28 / Chapter III. --- BRIEF REVIEWS OF THE TECHNIQUES USED IN THIS STUDY --- p.34 / Chapter III.1 --- Comparative Genomic Hybridization (CGH) --- p.34 / Chapter III.2 --- Loss of Heterozygosity (LOH) --- p.37 / Chapter III.3 --- Methylation-Specific PCR (MSP) --- p.38 / Chapter IV. --- OBJECTIVES OF STUDY --- p.40 / Chapter V. --- MATERIALS AND METHODS --- p.41 / Chapter V.l --- Sample Collections --- p.41 / Chapter V.1.1 --- Patients Information for CGH Studies --- p.42 / Chapter V. 1.2 --- Patients Information for LOH Studies --- p.42 / Chapter V.2 --- Extraction of Genomic DNA for Tumor and Normal Tissues --- p.47 / Chapter V.2.1 --- Extraction of Genomic DNA from Frozen Tissues or Paraffin Embedded Sections --- p.47 / Chapter V.2.2 --- Extraction of Genomic DNA from Blood --- p.48 / Chapter V.3 --- Comparative Genomic Hybridization (CGH) of Gastric Carcinoma --- p.49 / Chapter V.3.1 --- Preparation of Normal Metaphase Slides --- p.49 / Chapter V.3.2 --- Metaphase Slide Denaturation --- p.49 / Chapter V.3.3 --- Nick Translation for DNA Labeling --- p.50 / Chapter V.3.4 --- Dot Blot Assay for Biotin and Digoxigenin-Labeled DNA --- p.51 / Chapter V.3.5 --- "Probe Preparation, Denaturation and Hybridization" --- p.51 / Chapter V.3.6 --- Post hybridization Washing and Detection --- p.52 / Chapter V.3.7 --- Image Acquisition and Analysis of CGH Images --- p.53 / Chapter V.4 --- Loss of Heterozygosity (LOH) Analysis on Chromosome 6q --- p.55 / Chapter V.4.1 --- Microsatellite Markers --- p.55 / Chapter V.4.2 --- Polymerase Chain Reaction (PCR) --- p.57 / Chapter V.4.3 --- Denaturing Polyacrylamide Gel Electrophoresis --- p.58 / Chapter V.4.4 --- SYBR Gold Nucleic Acid Gel Staining and Image Viewing --- p.58 / Chapter V.4.5 --- Assessment of Loss of Heterozygosity (LOH) --- p.59 / Chapter V.4.6 --- Statistical Analysis --- p.61 / Chapter V.5 --- Methylation Specific Polymerase Chain Reaction (MSP) --- p.62 / Chapter V.5.1 --- Bisulfite Modification of DNA --- p.62 / Chapter V.5.2 --- Mehtylation Specific PCR --- p.63 / Chapter VI. --- RESULTS --- p.66 / Chapter VI.1 --- Results of CGH --- p.66 / Chapter VI. 1.1 --- Chromosomal Copy Number Aberrations in Gastric Carcinoma --- p.66 / Chapter VI. 1.2 --- Comparison of CGH Results with Intestinal and Diffuse Type of Gastric Carcinoma --- p.67 / Chapter VI.2 --- LOH Analysis of Chromosome 6q --- p.73 / Chapter VII. --- DISCUSSIONS --- p.83 / Chapter VII.l --- Discussions on CGH --- p.83 / Chapter VII.2 --- Discussions on LOH Study --- p.89 / Chapter VII.2.1 --- Two Distinct Deletion Regions --- p.89 / Chapter VII.2.2 --- Possible Candidate Suppressor Genes in Two Deletion Regions --- p.93 / Chapter VII.2.3 --- Infrequent Loss of IGF2R Gene --- p.95 / Chapter VII.3 --- Relationship Between Intestinal Metaplasia and Gastric Carcinoma --- p.99 / Chapter VII.4 --- Microsatellite Instability --- p.100 / Chapter VII.5 --- Correlations --- p.103 / Chapter VII.6 --- Comparison Between CGH and LOH Results on Chromosome 6 --- p.104 / Chapter VII.7 --- Conclusions --- p.106 / Chapter VII.8 --- Limitations of the Study --- p.107 / Chapter VII.8.1 --- Limitation of CGH --- p.107 / Chapter VII.8.2 --- Limited Information Supply by LOH Analysis --- p.107 / Chapter VII.8.3 --- Small Sample Size --- p.107 / Chapter VII.9 --- Future Studies --- p.108 / Chapter VIII. --- REFERENCES --- p.110

This thesis explores the variety of ways in which scientific views of genetic testing are portrayed in the realm of popular culture. As a case study, I have used the identification of the gene for hereditary stomach cancer which occurred in New Zealand in 1998, and was the result of a partnership between the affected whanau and scientists from the University of Otago. Both the empirical and theoretical findings of this project have shown how such accounts are not neutral or transparent. Rather, they are positioned to represent certain values and ideas, and this is even more evident when those affected are Maori. However, considering textual representations of the gene and cancer has revealed the importance of taking into account the fact that these 'things' are also physical and material. I consider the implications of this and consider the ways in which the whanau health workers negotiate the fetishism apparent in biomedicine. Despite its misgivings, biomedicine has immense benefits, some of which the whanau have manipulated and appropriated for their own good, although they do so on their own terms. Despite the many complexities involved in this case study, this is a positive and hopeful story where those involved in the stomach cancer gene project have emerged with improved solutions.

Thesis (Ph. D.)--University of Hong Kong, 2010. / Includes bibliographical references (leaves 181-207). Also available in print.

Thesis (M. Med. Sc.)--University of Hong Kong, 2001. / Includes bibliographical references (leaves 27-31).

Thesis (M. Phil.)--University of Hong Kong, 2001. / Includes bibliographical references (leaves 105-136).

published_or_final_version / Medical Sciences / Master / Master of Medical Sciences

published_or_final_version / abstract / toc / Pharmacology / Doctoral / Doctor of Philosophy