This research evaluates the effectiveness of using carbon dioxide (CO2) pressurization to extend strawberry fruit prep shelf life for the eventual use in yogurt applications. In this experiment, CO2 treatments of 5, 15, and 25 pounds per square inch were used as a processing step to inactivate microorganisms, which in turn could aid in the preservation and maintenance of product quality during storage thus improving consumer acceptance of the yogurt. Microbial levels of the fruit prep treatments were monitored over a six-week period by enumerating aerobic plate counts and yeast and mold levels. The color, pH, and texture of the treatments were also evaluated throughout the duration of the study. Sensory attributes of the product were evaluated by formal sensory panel at the beginning of the study to gather consumer feedback on potential changes introduced by the treatment to the finished product. For sensory analysis, the different CO2 treatments of fruit prep were mixed with plain yogurt and given to panelists. The different treatments were taken from one homogenous mixture of fruit prep and then were randomly divided into five different treatment groups: a control group, a thermally processed group, and the three different pressure levels of CO2. Results from the experiment showed that carbonation does not negatively impact product overall acceptability. Shelf life results showed that CO2 treatments are not effective in maintaining or extending the shelf life of strawberry fruit prep when compared to a thermal treatment.