Naps have been shown to benefit declarative memories in early childhood. This benefit has been associated with sleep spindles during the nap. However, whether young children’s naps and their accompanying physiology benefit other forms of declarative learning is unknown. Using a novel storybook task, we found performance was better following a nap compared to performance following an equivalent interval spent awake. Moreover, performance was better the following day if a nap followed learning. Further, change in post-nap performance was positively associated to the amount of time spent in slow wave sleep. This suggests that slow wave sleep in naps may support episodic memory consolidation in early childhood. Taken in conjunction with prior work, these results suggest that multiple features of brain physiology during naps may contribute to declarative memory processing in early childhood.

Emotion is thought to modulate the long-term fate of memories. Experiences that elicit an emotional response tend to be better remembered than comparatively unemotional events, while the emotional charge associated with these memories diminishes over time. Sleep – in particular rapid-eye movement (REM) and slow-wave sleep (SWS) - has been implicated in both the selective strengthening and affective uncharging of emotional memories. According to the sleep to forget, sleep to remember (SFSR) hypothesis, both processes occur in parallel during REM sleep. Although evidence strongly supports a role of REM sleep in the selective consolidation of emotional memories, it is far less clear to what extent sleep is involved in the development of emotional charge. While some studies support a primary role of REM in habituation (i.e. the decrease of emotional charge), others suggest a more central role of SWS. Further, existing literature indicates that the physiological (bottom-up) and cognitive (top-down) components of emotional responses may be differentially processed across sleep. Chapter 2 proposes complementary functions of REM and SWS in emotional memory processes based on a combination of evidence from rodent and human research. The experiments presented in this thesis employed polysomnography (PSG), subjective arousal testing, pupillometry, targeted memory reactivation (TMR), and transcranial alternating current stimulation (tACs) to investigate the respective roles of REM and SWS in the overnight development of subjective and autonomic arousal in response to negative emotional and neutral stimuli. In Chapter 3 I assessed how subjective and autonomic responses to neutral and negative stimuli develop across 12 hours containing either nocturnal sleep or daytime wakefulness. I found that autonomic reactivity – indexed by pupil dilation – decreased across sleep but not wake, while subjective arousal did not change across either interval. In a further experiment, I investigated whether the placement of sleep within a 24 hour interval would affect habituation. Once again, autonomic arousal decreased significantly. Subjective arousal towards negative stimuli was found to decrease more if sleep followed rather than preceded daytime wakefulness within the 24 hour interval. In Chapter 4 I explored the role of REM sleep in emotional habituation by applying 5 Hz tACs in an attempt to entrain endogenous cortical theta (4-7 Hz) activity, which has previously been associated with emotional memory consolidation in humans. Surprisingly, I found that stimulation was associated with a reduction in theta power and no change in subjective or autonomic habituation compared to the sham control night. In Chapters 5 and 6, I addressed the contribution of memory reactivations during SWS and REM sleep, respectively, in emotional habituation using TMR. In Chapter 5, I found that TMR was associated with a decrease and simultaneous increase in autonomic habituation towards negative and neutral stimuli, respectively, without affecting overnight changes in subjective arousal. In contrast, in Chapter 6, TMR during REM sleep was associated with an increase in subjective habituation towards both neutral and negative stimuli without affecting autonomic responses. In conclusion, my results provide new insights to the role of sleep in emotional habituation. I have provided evidence that targeted memory reactivation during REM sleep can modulate the development of cognitive evaluations of emotion, while TMR during SWS may interfere with autonomic habituation. This suggests distinct emotional processing during REM and SWS, as well as a dissociation between subjective and autonomic habituation across sleep. These results are discussed in the light of previous research and the model of sleep-dependent emotional memory processing proposed in Chapter 2.

The method targeted memory reactivation (TMR) uses specific stimulation when subjects are completing tasks and during sleep. The TMR process is known to influence the consolidation of declarative memories. The aim of this thesis is to conduct a systematic review on the effects of TMR on declarative memory consolidation during slow-wave sleep (SWS). The research question is to answer what effect TMR during SWS has on the consolidation of declarative memory in healthy humans when presented with associated cues of the targeted learning experiences. Eighteen studies were included in this review. Four studies found a significant effect of TMR on declarative memory consolidation, and 10 found a non-significant effect. In four studies the effect of TMR depended on different inclusions, analyses, and factors, for example between slow oscillation up-and down-states and between participants that vary in pre-sleep performance in the examined task. In contrast to previous findings, this review does not provide evidence for the effect of TMR on declarative memories during SWS. More research analysing different factors, such as different cues, age of participants, duration of SWS, and specific experimental tasks, needs to be done in the fields of TMR and auditory cues.

Made available in DSpace on 2014-12-17T14:56:17Z (GMT). No. of bitstreams: 1 EdsonAMRB_DISSERT.pdf: 7502344 bytes, checksum: 78d70443ae2fd9033fe78b23c5cbd811 (MD5) Previous issue date: 2013-07-30 / Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior / Complex network analysis is a powerful tool into research of complex systems like brain networks. This work aims to describe the topological changes in neural functional connectivity networks of neocortex and hippocampus during slow-wave sleep (SWS) in animals submited to a novel experience exposure. Slow-wave sleep is an important sleep stage where occurs reverberations of electrical activities patterns of wakeness, playing a fundamental role in memory consolidation. Although its importance there s a lack of studies that characterize the topological dynamical of functional connectivity networks during that sleep stage. There s no studies that describe the topological modifications that novel exposure leads to this networks. We have observed that several topological properties have been modified after novel exposure and this modification remains for a long time. Major part of this changes in topological properties by novel exposure are related to fault tolerance / A an?lise da topologia de redes ? uma poderosa ferramenta no estudo de sistemas complexos tal como as redes cerebrais. Este trabalho procura descrever as mudan?as na topologia de redes de conex?o funcional em neur?nios do c?rtex sensorial e do hipocampo durante o sono de ondas lentas (SWS) em animais expostos ? novidade. O sono de ondas lentas ? um importante estado do sono onde h? reverbera??o de padr?es de atividade el?trica ocorridos na vig?lia, tendo com isso papel fundamental na consolida??o de mem?ria. Apesar de sua import?ncia ainda n?o h? estudos que caracterizam a din?mica da topologia de redes de conex?o funcional durante este estado. Tampouco h? estudos que descrevem as modifica??es topol?gicas que a exposi??o ? novidade traz a essas redes. Observamos que v?rias propriedades topol?gicas s?o modificadas ap?s a exposi??o ? novidade e que tais modifica??es se mant?m por um longo per?odo de tempo. A maior parte das propriedades modificadas pela exposi??o ? novidade est? relacionada ? toler?ncia ? falha

The functions of sleep remain enigmatic. One of the dominant, yet more contentious hypotheses is that sleep is involved in memory consolidation. A large body of evidence supports the role of rapid eye movement (REM) sleep in memory consolidation, especially in rodents. In humans, the role of REM sleep in memory consolidation has also been investigated, however it is unclear if it supports only one type of memory, or consolidation for several memory systems. Recent evidence suggests that non-REM is also involved in memory consolidation. The role of theta activity during REM and sleep spindles during non-REM may provide electrophysiological signatures reflecting memory consolidation processes. The studies presented here attempt to further investigate the electrophysiological characteristics of the learning-dependent changes in REM and slow wave sleep (SWS) in rats. A 2-stage model of memory consolidation is outlined here, and both steps of the model were investigated. Consistent with previous studies, REM increases were observed following avoidance training. During this period, theta power during REM sleep was increased compared to non-learning rats. Increased sleep spindle density during SWS was observed following REM increases. When REM sleep was suppressed by infusing the GABAB agonist baclofen into the pedunculopontine nucleus, avoidance performance acquisition was impaired. Baseline sleep spindles predicted whether rats were able to learn to make avoidance responses. Results suggest that both REM and SWS may be sequentially involved in memory consolidation processes. Discrete periods (windows) exist for REM and SWS when memory consolidation processes appear to take place. Theta activity during REM sleep from 17- 20 h on the first post-training day and sleep spindles during SWS from 21-24 h on the first post- training day are increased in learning rats and are related to memory performance. / Thesis (Ph.D, Neuroscience Studies) -- Queen's University, 2009-10-26 12:07:47.515

Through continued innovation and growth, traveling wave tube amplifiers (TWTAs) remains the microwave power amplifiers of choice in a wide range of high power microwave and millimeter-wave applications specifically for aerospace applications with the volume, weight, bandwidth and power constraints. These advances can be credited to device innovation, improved modeling and design and development of advanced materials and construction techniques. This thesis aims at advancing the present technology of TWTs with coupled resonator slow-wave structures (SWSs) by a combination of device innovation, development of enhanced analytical and field analysis codes and understanding gained through improved modeling, simulation and experimentation. In a TWT, the SWS that slows the RF wave velocity down to near the electron beam velocity for interaction with the electron beam primarily determines the microwave performances of the tube. As compared to helix SWS, the coupled resonator SWS is capable of handling high peak and average powers with higher efficiency and TWTs based on these SWS are well suited for air-borne or space-borne radar systems and the major focus of this thesis is on the analysis and design of coupled resonator SWSs. As a part of this thesis, improved analytical codes based on quasi-TEM analysis and equivalent circuit analysis have been developed. The technical formulation is explained and the improvements made for enhanced accuracy and for incorporation of different types of coupled resonator SWSs detailed. Using these models new variants of coupled resonator SWSs have been investigated. The SWSs proposed are the ladder-core inverted slot mode SWS and the inductively loaded inter digital SWS (ILID-SWS). The possibility of achieving both coalesced mode design that gives wide bandwidth and multi beam design that improves the peak power and gain using rectangular ILID-SWS is presented. The properties of these proposed SWSs have been compared with the existing SWSs and found to give superior performance. Also an improved modeling and simulation technique using 3-D electromagnetic codes has been proposed and the conventional cold test measurement procedure has been modified for more accurate results. Numerous illustrative examples are presented throughout the thesis highlighting the analytical model and simulation code validation with experimental results. The experimentations have been carried out on the real SWS model that have been fabricated and assembled. Further, the contribution of the thesis is towards the development of a field analysis model for analysis of a corrugated waveguide SWS, based on the coupled integral equation technique (CIET), which is a combination of mode matching technique (MMT) and method of moments. The technical formulation and computational methodology employed in the model are explained and some of the most important aspects of implementation like the handling of singularities and choice of parameters controlling the accuracy is discussed. The accuracy and speed of the code is demonstrated by comparing CIET with MMT and 3-D electro magnetic simulators based on finite difference time domain (FDTD) method and finite element method (FEM). The CIET code developed is quite faster than the existing numerical methods and helps in solving the convergence problem associated with the MMT.

Vacuum microwave tubes, such as klystron, traveling-wave tube, gyrotron are high efficiency devices, where the RF interaction structure facilitates efficient energy transfer from the kinetic energy of the high energy electron beam to the electromagnetic wave. Traveling-wave Tube is the most versatile microwave power amplifier widely used for terrestrial communication, radar and aerospace applications. The waveguide based slow-wave structures like Millman, Karp, inter digital, grated waveguide, ring-plane, ring-bar, millitron and folded-waveguide structure gathered importance for application in millimeter-wave traveling-wave tubes. Among these millimeter-wave interaction structures, the folded-waveguide slow-wave structure became the most popular due to its robust structure, high power capability, low RF loss, simpler coupling, reasonably wide bandwidth and ease of fabrication for millimeter-wave to terahertz frequencies. Hence this thesis aims to analyse the folded-waveguide slow-wave structure for broad-banding and efficiency enhancement. The existing approaches for the analysis of cold circuit parameters (dispersion and interaction impedance characteristics) of folded-waveguide slow-wave structure are reinvestigated and found that these have limitation, as the effects of E-plane bend and beam-hole discontinuities are ignored in the parametric analysis. A cascaded matrix equivalent circuit model includes the effect of E-plane and beam-hole discontinuities for the analysis, but reported only for the serpentine folded-waveguide slow-wave structure. The cold test measurement technique was reported only for the dispersion characteristics. Hence the measurement technique has to be extended for the measurement of interaction impedance. The author proposes to orient the present doctoral work to (i) extend the proposed cascaded transmission matrix equivalent model for the analysis of rectangular folded-waveguide slow-wave structure, (ii) develop a non-resonant perturbation technique for the measurement of interaction impedance characteristics of the folded-waveguide slow-wave structure and also to (iii) establish new analysis models for the folded-waveguide slow-wave structure. The effect of E-plane bend and beam-hole discontinuities on the RF characteristics have been considered and simple, yet accurate closed form expressions for the computation of dispersion and interaction impedance characteristics have been established by three different approaches namely: transmission line equivalent circuit model, conformal mapping equivalent circuit model and quasi-TEM approach. The analysis results are benchmarked against 3-D electromagnetic modeling. The non-resonant perturbation theory is developed for the interaction impedance measurement. Typical Ka-band structures are fabricated by wire-EDM process and cold test measurements are carried out to benchmark the analysis approaches. The equivalent circuit models based on lumped circuit model are simpler than the cascaded matrix equivalent circuit model and can give closed form expressions for the prediction of dispersion and interaction impedance characteristics. The quasi-TEM approach can be extended for the complicated structure like ridge-loaded FWG-SWS. Broad-banding of the conventional folded-waveguide slow-wave structure is attempted by ridge-loading on the broad wall of the structure. The ridge-loaded folded-waveguide slow-wave structure is analyzed by parametric approach, cascaded transmission matrix equivalent circuit model and quasi-TEM approach and validated against numerical simulation. The analysis is extended for exploring the efficacy of the ridge-loading on broad-banding of the traveling-wave tube. Finally efficiency enhancement of the folded-waveguide slow-wave structure is attempted by introducing grating on the broad wall of the structure. The analysis is carried out by numerical simulation for exploring the efficacy of the grating on efficiency enhancement of the traveling-wave tube.