In my thesis, I decided to describe how all the means that actor?s body and soul comprise can by synthesized into something that is able to tell a story, communicate and be passed on. I myself am still in the middle of the process of discovering all the separate particles of those means, as well as of how they function, how they can be used and how they can be worked with. During my studies, and moreover during my lifelong encounter with theatre and art, this topic has intrigued me and I have always come across it to some extent. That is why in my thesis I focus not only on the already discovered andexamined kind of synthetic stage appeal, but also on the undiscovered one. Because a man himself represents his one and only primordial universe and his own utterly first teacher, through whom every perception has to pass. And it goes differently with every single person. Whether the person is an actor, a ballet girl, or a singer. In the first part of my thesis, I mainly focus on the historical overview of theatre?s origin and function and I follow the evolution of the so-called synthetic acting. Furthermore, I present several individual personalities, theatrical groups and their artistic movements, who later explored the concept of synthetic acting intentionally. To sum up, I try to reflect my own experience with synthetic acting and, in the end of my thesis, I concentrate on my search for my very own form of synthetic acting.

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Ligninolytic systems found their application in the food industry, but they are also used for the degradation of several xenobiotic compounds and dyes. This characteristic is making them be a useful tools for bioremediation purposes, that is why the interest in ligninolytic enzymes is increasing. The main aim of practical part was preparation of immobilized enzyme lignin peroxidase by method CLEA from fungus Piptoporus betulinus, Trametes gibbosa and their cocultivation. Activities of free and immobilized enzymes from these fungus were compared and measured by UV/VIS spectrometer. Conditions of precipitation and immobilization were specified by method CLEA. pH optimum for proteins precipitation were for Piptoporus betulinus pH 7,0, for Trametes gibbosa pH 5,0 and for cocultivation pH 6,0. The best concentration of glutaraldehyd, as a crosslinking agent, was for Piptoporus betulinus and cocultivation 50mM and for Trametes gibbosa 10mM. By immobilization, the activity of all enzymes were successfully increased up by 20 %. After optimalization of CLEA enzymes preparation, other parameters such as pH optimum, stability and temperature optimum and stability were tested. All enzymes had their pH optimum in acidic to slightly acidic environment and temperature optimum was in range from 30 °C to 40 °C. Immobilized CLEA enzyme from cocultivation was the most stable for all the enzymes. Free and CLEA enzymes were tested on synthetic food dyes, while their biodegradable ability was examined. Decrease of dyes was measured on HPLC with DAD detector. The sorption of fungi play an important role in this experiment, because fungi created mycelium and partially sorbed the dye. From this point of view, higher decolorization of dye in free enzymes in comparison with CLEA was observed. The best ability of decolorization was shown by a free enzyme from a Trametes gibossa, which after 14days of cultivation decolored more than 90 % dye. In total, significant decolorization (more than 80 % during 14 days) was achieved for azorubin. Degradation of dyes by CLEA was not so effective, which could be possibly caused by cultivation of enzymes without their substrate (veratrylalcohol). Cocultivation technique is very promising method how to increase LiP activity and concurrently create enzyme with improved properties.

This master’s thesis is focused on evaluation of synthetic musk fragrances using ecotoxicity tests. Four musk fragrances were tested – Galaxolide, Tonalide, Musk ketone and Musk xylene. Four alternative ecotoxicity tests with living organisms, one standard ecotoxicity test and three fytotests were used for evaluation of ecotoxicity. Alternative tests were performed on crustaceans Daphnia magna and Thamnocephalus platyurus, brine shrimp Artemia salina and rotifer Brachionus calicyflorus. Root growth inhibition test of Sinapis alba, root growth inhibition test of Lactuca sativa, root growth inhibition test of Allium cepa and growth inhibition test of Lemna minor were performed in the terms of fytotests. On the basis of the results the values of EC50, IC50 and LC50 were determined and the ecotoxicity of musk compounds was evaluated.

The aim of the thesis was to develop an algorithm to generate synthetic XML documents to cover the resolution of an XPath query with test cases, with a special emphasis on the ease of configuration. We have also implemented a prototype, optimized its performance and demonstrated its properties by running it on sample data. Powered by TCPDF (www.tcpdf.org)

There is a rise of new phenomenon in drug use in the last few years. There has been increase in the incidence of new synthetic drugs, or the so-called "legal highs" and their use in the general population became more frequent. Legal highs are substances that mimic the effects of illegal drugs, but they are not controlled by the United Nations Single Convention on Narcotic Drugs of 1961 or the UN Convention on Psychotropic Substances of 1971. These substances can produce comparable risks and harms simmilar to the illegal drugs (EMCDDA, 2009c). The aim of research conducted for the purpose of this thesis is to reveal how the risks associated with the use of these products are percieved by their users, whether their legal status has any influence on the perception of their safety and how do the users minimize risks that may be associated with the use of these substances Qualitative research carried out for the needs of graduate work used data obtained by two methods. The first method was a content analysis of online discussion forums of new synthetic drugs users. 16 online discussion forums with a total number of 832 posts were analyzed. The second method were semi-structured interviews with users of new synthetic drugs. They were conducted with 9 respondents. Regarding the perception of risk, some...

Products of daily use, which are mainly cosmetics and personal care products could significantly affect the environment. Recently, in terms of environmental protection, organic compounds responsible for the pleasant smell of these products - synthetic fragrances or musk compounds - are often mentioned. This diploma thesis aims to assess the ecotoxicity of several new substances from the group linear (alicyclic) musk compounds. From this group following fragrance ingrediens were tested: lilial, linalool, arocete and aroflorone. Since the majority of their residues enter the aquatic ecosystem, aquatic freshwater crustacean Daphnia magna and Thamnocephalus platyurus and aquatic plant Lemna minor were selected as test organisms. Individual ecotoxicity of above mentioned substances was evaluated as well as toxicity of binary mixture of arofloron and linalool on organisms Daphnia magna and Thamnocephalus platyurus. Based on the test results the ecotoxicological values LC50, EC50 and IC50 for the tested substances were evaluated and their ecotoxicity was compared. Lilial showed the highest ecotoxicity. Value of 24h EC50 for organism Daphnia magna was 2.13 mg/l and value of 24h LC50 for the organism Thamnocephalus platyurus was 10.86 mg/l.

PALICH, K. The use of fixed-base simulators in pilot training. Brno: Brno university of technology, Faculty of mechanical engineering, 2013. 80 s. Diploma thesis head: Ing. Jiří Chlebek, Ph.D. This diploma thesis deals with aircraft simulators and syntethic training devices which are used for pilot training. One of the chapters consist of division of syntethic training devices, which is defined by ordinance. In the next chapter there is general description of the basic function of those devices, history of simulators and their development from the very beggining to the present. A significant part of this diploma thesis is about flight simulator Jeppesen Flite Pro, which is at Institute of Aerospace at VUT. In the following chapters there is information about usage of this simulator in the education at this institute and there are picked specific subjects, where this simulator can be used.

Unfolded protein response (UPR) is a complex cellular mechanism induced upon ER stress caused by various environmental factors. Single spanning signal transducers of UPR were reported to recognise also lipid-induced ER stress. Studies of these transducers, namely PERK and IRE1 uncovered that they can sense change in membrane properties and activate themselves by clustering. Moreover, signal transducer IRE1 retained ability to sense changes in the membrane properties with TMD exchanged for a polyLeu α-helix. It was thus unclear what mechanism drives lipid-induced UPR via IRE1. We employed model membrane system in form of LUVs, where properties of membranes can be readily altered by specific lipid composition. As a simplified model of the UPR signal transducers in the ER, synthetic transmembrane peptides with polyLeu core were used. Dynamic light scattering (DLS) has been used for qualitative and semi-quantitative analysis of LUVs. Clustering of synthetic peptides was determined by time resolved anisotropy of fluorescence. DLS results demonstrate successful formation of vesicles with a desired size in all planned composition. On the contrary to the studies in living cells, the presence of cholesterol or palmitic acid in model membranes did not induce the aggregation of transmembrane peptides....

The dissertation thesis about Czech and Polish legislation on drug offenses deals with driving under the influence of addictive substances, responsibility for acts committed under the influence of addictive substances, drug possession, cannabis cultivation, production and distribution of drugs, handling of articles for drug production and spreading drug addiction. The aim of this work is to bring a critical view of the current Czech legislation on drug offenses and related issues and then to present specific legislative proposals based on comparison with Polish law. The thesis analyzes the Polish and Czech legal regulations of drug offenses and brings a comparison of the facts of the aforementioned crimes and specific case studies. The work is based on legal regulations and case law of both countries, practical knowledge and also available statistical data. The thesis deals with the legalization of drugs comprehensively, including the arguments for and against legalization, wich are used in the Czech Republic and Poland. It addresses the legalization of cannabis on the example of Holland, the USA and Uruguay, as well as the availability of hard drugs in substitution programs in the Czech Republic and Poland. In conclusion the thesis gets to the explanation how the Czech legislator should proceed...