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The molecular battle between virulence weapons of Pseudomonas syringae and integrated defense responses of Arabidopsis thalianaKim, Min Gab, January 2006 (has links)
Thesis (Ph. D.)--Ohio State University, 2006. / Title from first page of PDF file. Includes bibliographical references (p. 103-124).
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Investigating the Evolution and Functional Diversification of Pseudomonas syringae type III effector HopZ1Yea, Carmen 04 January 2012 (has links)
The pathogenicity of plant pathogen Pseudomonas syringae depends on the type III secretion system which translocates effector proteins into host cells. In response, plants have evolved resistance proteins to detect presence of specific effectors and activate defense responses. The constant host surveillance imposes a strong selective pressure on effector proteins to evolve rapidly in order to evade detection. The P. syringae HopZ1 effector has evolved into three allelic forms as a result of diversifying selection. In this thesis, I aimed to investigate how sequence divergence contributes to the distinct allelic specificities of HopZ1. Mutational analysis of HopZ1a identified three amino acid residues that were potentially involved in dampening host defense responses, and two HopZ1a mutants partially lost the ability to trigger defense responses yet did not lose their virulence functions. These results suggested that distinct host targets could be involved in the defense-eliciting activity and virulence function of HopZ1a.
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Investigating the Evolution and Functional Diversification of Pseudomonas syringae type III effector HopZ1Yea, Carmen 04 January 2012 (has links)
The pathogenicity of plant pathogen Pseudomonas syringae depends on the type III secretion system which translocates effector proteins into host cells. In response, plants have evolved resistance proteins to detect presence of specific effectors and activate defense responses. The constant host surveillance imposes a strong selective pressure on effector proteins to evolve rapidly in order to evade detection. The P. syringae HopZ1 effector has evolved into three allelic forms as a result of diversifying selection. In this thesis, I aimed to investigate how sequence divergence contributes to the distinct allelic specificities of HopZ1. Mutational analysis of HopZ1a identified three amino acid residues that were potentially involved in dampening host defense responses, and two HopZ1a mutants partially lost the ability to trigger defense responses yet did not lose their virulence functions. These results suggested that distinct host targets could be involved in the defense-eliciting activity and virulence function of HopZ1a.
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Characterization of a type vi secretion system and related proteins of pseudomonas syringaeRecords, Angela Renee 15 May 2009 (has links)
Pseudomonas syringae is a pathogen of numerous plant species, including
several economically important crops. P. syringae pv. syringae B728a is a resident on
leaves of common bean, where it utilizes several well-studied virulence factors,
including secreted effectors and toxins, to develop a pathogenic interaction with its host.
The B728a genome was recently sequenced, revealing the presence of 1,297 genes with
unknown function. This dissertation demonstrates that a 29.9-kb cluster of genes in the
B728a genome encodes a novel secretion pathway, the type VI secretion system (T6SS),
that functions to deliver at least one protein outside of the bacterial cell. Western blot
analyses show that this secretion is dependent on clpV, a gene that likely encodes an
AAA+ ATPase, and is repressed by retS, which apparently encodes a hybrid sensor
kinase. RetS and a similar protein called LadS are shown to collectively modulate
several virulence-related activities in addition to the T6SS. Plate assays demonstrate that
RetS negatively controls mucoidy, while LadS negatively regulates swarming motility. A mutation in retS affects B728a population levels on the surface of bean leaves. A
model for the LadS and RetS control of B728a virulence activities is proposed, and
possible roles for the B728a T6SS are addressed.
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Genetic Dissection of Virulence and Immune-eliciting Functions and Characterization of the Immune Response of the Pseudomonas syringae HopZ1 Type III Effector FamilyRizzolo Roustayan, Kamran Daniel 17 July 2013 (has links)
Successful pathogens like Pseudomonas syringae translocate type III effector proteins (T3SE) into host cells. Plant hosts react by specifically recognizing these effectors via R proteins that trigger defense responses. The T3SE family HopZ1 has evolved into three allelic forms as a result of diversifying selection. In this thesis, I investigated how virulence and immune-eliciting functions are determined in HopZ1a and HopZ1b in Arabidopsis. Mutational analysis of HopZ1a identified ten residues important for immune elicitation and at least three are involved in virulence functions. These results suggest that distinct key amino acid residues in HopZ1a mediate the two activities. The closely related HopZ1b T3SE elicits an inconsistent immune response in Arabidopsis. We found that HopZ1b-triggered immune response involves a TIR-type R protein and plastid-derived SA. Together, these results highlight an uncharacterized ETI response to the HopZ1 family of T3SEs.
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Pseudomonas on peas : ice nucleation, identification and pathogenicity/ by Mitra Mazarei.Mazarei, Mitra January 1991 (has links)
Copies of author's previously published articles inserted. / Bibliography :leaves 65-80 / x, 80, [64] leaves, [24] leaves of plates : ill. (some col.) ; 30 cm. / Title page, contents and abstract only. The complete thesis in print form is available from the University Library. / Ice nucleation active (INA) bacteria were detected in a pea field in South Australia. They were identified as strains of Pseudomonas syringae and Pseudomonas flourescens biotype 1. Some chemical agents were tested on the two ice nucleating species, as cryoprotectants. / Thesis (Ph.D.)--University of Adelaide, Dept. of Crop Protection, 1991
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Pseudomonas on peas : ice nucleation, identification and pathogenicity /Mazarei, Mitra. January 1991 (has links) (PDF)
Thesis (Ph. D.)--University of Adelaide, Dept. of Crop Protection, 1991. / Copies of author's previously published articles inserted. Includes bibliographical references (leaves 65-80).
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Overwintering biology of the tarnished plant bug, Lygus lineolaris (Palisot de Beauvois) (Hemiptera: miridae), in Nova Scotia, and the potential use of ice nucleating active bacteria for insect pest control /Sheffield, Cory Silas. January 1900 (has links) (PDF)
Thesis (M.Sc.)--Acadia University, 1998. / Includes bibliographical references (leaves 65-80). Also available on the Internet via the World Wide Web.
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Overwintering biology of the tarnished plant bug, Lygus lineolaris (Palisot de Beauvois) (Hemiptera: miridae), in Nova Scotia, and the potential use of ice nucleating active bacteria for insect pest controlSheffield, Cory Silas. January 1900 (has links)
Thesis (M.Sc.)--Acadia University, 1998. / Includes bibliographical references (leaves 65-80). Also available on the Internet via the World Wide Web.
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DNA-Array-Technologie Entwicklung von DNA-Arrays mit 13.000 cDNA-Klonen des Modellorganismus Arabidopsis thaliana und Anwendung in der Genexpressionsanalyse pflanzlicher Pathogenabwehr /Scheideler, Marcel. Unknown Date (has links) (PDF)
Universiẗat, Diss., 2001--Mainz. / Auch als gedr. Diss.
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