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Tannin/protein interactions : effects on digestibility coefficients and endogenous losses in broilersMansoori, Behzad January 1999 (has links)
Tannic acid was shown to increase weight loss, and total excretion of endogenous dry matter, nitrogen, uric acid, amino acids, energy and minerals (calcium, magnesium and phosphorus) in broilers. There was a positive linear correlation between the amount of tannic acid dosed, and the weight and endogenous losses. A variation in the excretion of individual amino acids and minerals in response to dosed tannic acid was also noted. The mechanisms of increased endogenous losses of the birds as influenced by tannic acid were investigated and discussed. Feeding gelatin and casein significantly reduced the adverse effects of tannic acid on weight and endogenous losses. There were also significant improvements in digestibility coefficients and metabolisable energy values of protein in tannic acid dosed birds when the amount of the protein increased. Although in birds not dosed with tannic acid, casein had a higher digestibility and metabolisable energy compared to gelatin, tannic acid dosed birds showed greater improvements in digestibility coefficients when receiving gelatin. The above contrast was possible because of the higher affinity of gelatin for tannic acid compared to the casein. Polyethylene glycol (PEG) was shown to alleviate the weight and endogenous losses of tannic acid dosed birds not receiving protein. Polyethlene glycol also reduce the endogenous losses as well as improved the digestibility coefficients and metabolisable energy values of tannic acid dosed birds when receiving the lower amounts of gelatin (6g) or casein (6 and 12g). However, the effect of PEG was less apparent in tannic acid dosed birds receiving the higher amounts of gelatin (12 and 18g) and casein (18g). In conclusion, the presence of tannins in poultry diets are likely to increase the endogenous losses of organic materials as well as minerals, but this is likely to depend on the type and amount of diet ingredients such as proteins and additives (eg. PEG).
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The development of a continuous solid-state fermentation process for the production of fungal tannaseVan de Lagemaat, Juergen January 2001 (has links)
No description available.
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Influence of Fresh and Processed Tannic Acid, and Fresh Tannic Acid, and Fresh Tannic Acid Plus Phosphate on Catfish Fillet Color, Microbial Shelf Life and OxidationCury, Guilherme Filizzola 11 August 2012 (has links)
Fresh tannic acid (5%) (FTA), heated to 121C for 15 min (PTA), and the combination of FTA and phosphates (FPH) were vacuum tumbled with catfish fillets and compared to water tumbled fillets (CTL). Fillets treated with FTA or PTA had higher whiteness whereas all treated fillets had higher chroma values than CTL, resulting in a deeper yellow color fillet. Microbial shelf life (CTL, log CFU/g, APC) of FTA and PTA treated fillets was extended by two days, to11.8 d at 4C, but FPH fillets did not reach over 5 log CFU/g for 15 d of storage. Fat from FTA and PTA treated fillets had induction points (IP) of 4.0 and 3.4 h, respectively, whereas FPH and CTL samples had IP of 1.4 and 2.4 h. Thus, FPH seems to be the best antimicrobial treatment and FTA/PTA, the best antioxidant treatment.
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Inhibitory effect of tannic acid and it¡¦s related compounds on DNA damage in human lymphocytes exposed to H2O2 and food mutagensChu, Cheng-Chang 28 July 2003 (has links)
Abstract
The effect of tannic acid (TA), gallic acid (GA), propyl gallate (PA) and ellagic acid (EA) on DNA damage in human lymphocytes induced by food mutagens, 3-amino-1-methyl-5 H-pyrido (4,3-b)indole (Trp-P-2) and 2-amino-1-methyl-6-phenylimadazo (4,5-b)pyridine (PhIP), and/or H2O2 was evaluated using single-cell electrophoresis (comet assay). The toxicity of these tested compounds on lymphocytes was not found. These compounds did not cause DNA damage at lower concentration of 0.1-10 µg/ml. At a concentration of 100 µg/ml, TA and GA exhibited slight DNA damage, whereas PA and EA showed no DNA damage. TA and its related compounds decreased the DNA damage induced by Trp-P-2 or PhIP at a concentration of 0.1-10 µg/ml. Moreover, the inhibition of H2O2-induced DNA damage increased with increasing concentrations up to 10 µg/ml. DNA repair enzymes, endonuclease III (Endo III) and formamidopyrimidine-DNA glycoslase (FPG) were used to examine the levels of oxidised pyrimidines and purines in DNA damage induced by H2O2, respectively. All the compounds at 10 µg/ml can reduce the level of FPG sensitive sites. However, only EA inhibited the formation of EndoIII sensitive sites. The results indicate that these compounds can enhance lymphocyte resistance towards DNA damage induced by food mutagens or H2O2.
Keywords: Tannic acid; Human lymphocyte; Comet assay; Hydrogen peroxide
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Polymers in microfluidicsBarrett, Louise M. January 2004 (has links)
There is great interest in miniaturized analytical systems for life science research, the clinical environment, drug discovery, biotechnology, quality control, and environmental monitoring and numerous articles have been written which predict the success of microfluidic based systems. It was demonstrated in this work that a microfluidic flow system could be quickly and easily manufactured in a research lab environment without the need for clean room facilities. The microfluidic device was created using polymethylmethacrylate, a CO2 laser and a standard oven. The device was designed, manufactured and ready for use within three hours. This work also investigated a chemiluminescent system which was intended for use in protease assays in the microfluidic device. This work also focused on the use of photoinitiated polymer monoliths, with immobilized tannic acid, as protein preconcentrators. The function of the monolithic devices was demonstrated by pumping low concentration solutions of BSA BODIPY® FL through the monolith. Both loading and elution were done using pressure. It was shown that BSA could be concentrated on and successfully eluted from the monolith. The elution volume for a 125 nl monolith was found to be 4 μl. Therefore an injection of a 60 μl sample of 1 x 10⁻⁹M BSA BODIPY ® FL gave rise to a concentration factor of 15. The pH optimum for the binding of BSA BODIPY ® FL was found to be pH 8.0 and the loading capacity of the tannic acid monolith was found to be 0.6 mg.ml⁻¹.
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Otimização da produção de mudas clonais de eucalipto com o uso de bioestimulantes / The optimization to producing eucalyptus clones with biostimulantsLeone, Gabriela Ferraz 15 March 2019 (has links)
Devido à grande importância que o gênero Eucalyptus apresenta, cada vez mais estudos relacionados à otimização da propagação de espécies do gênero vêm sendo conduzidos. Os principais entraves estão relacionados à sua clonagem e a recalcitrância que algumas espécies apresentam ao enraizamento e, por essa razão, o presente trabalho teve como objetivo avaliar o uso de bioestimulantes na mini e microestaquia de Eucalyptus spp. O presente trabalho se preocupou com o desenvolvimento de protocolos que não alteram excessivamente a prática empregada pelas empresas silviculturais. Para tanto, o trabalho foi dividido em 4 partes, sendo a primeira composta por uma revisão sobre enraizamento (item 2), visando elucidar todos os principais fatores que podem influenciar neste evento morfogênico. A partir desta revisão, se iniciaram estudos práticos com espécies e híbridos do gênero. O primeiro estudo (item 3) baseou-se na avaliação da morfofisiologia de miniestacas de três espécies de eucalipto (E. urograndis, E. benthamii e E. urophylla) em contato com AIB em pó e subsequente aplicação de tratamentos com bioestimulantes (ácido tânico e Algaren BZn®) utilizados de forma isolada e/ou em associação diretamente na base das miniestacas. O segundo estudo (item 4) avaliou a atuação destes mesmos tratamentos com bioestimulantes, em microcepas de Eucalyptus urograndis em fase de alongamento in vitro das brotações e sua posterior aclimatização. Por fim, o terceiro estudo (item 5) foi baseado na utilização dos tratamentos com bioestimulantes em microestacas de E. urophylla em fase de aclimatização em miniestufas, sendo os bioestimulantes aplicados na base das microestacas e na base das miniestufas (diretamente na bandeja). Em todos os experimentos foram coletados dados referentes aos parâmetros morfofisiológicos e, observou-se no primeiro e segundo experimento, que o ácido tânico na concentração de 250mg/L, foi o melhor tratamento empregado. Já, para o terceiro experimento, o Algaren BZn® na concentração de 1ml/L, apresentou resultados mais favoráveis. De acordo com as análises histológicas da rizogênese para todos os experimentos, verificou-se que as raízes adventícias desenvolvidas apresentaram origem cambial e conexão direta com o sistema vascular da parte aérea, indicando serem funcionais. O que permitiu constatar que, de forma geral, a adição de bioestimulantes otimizou a produção de mudas clonais de Eucalyptus. / According to the great importance that Eucalyptus genus presents, more studies are related to genus species propagation optimization have been conducted. Being the rooting recalcitrance that some species presents the main obstacle related to its cloning, this work had the aim to evaluate the biostimulants use in mini and microcuttings rooting process of Eucalyptus spp. For this, the present work was concerned with the protocols development that does not excessively alter the practice employed by silvicultural business. For this, the work was divided into 4 parts, the first have a rooting review (item 2), in order to elucidate all the main factors that may influence this morphogenic event. From this review, practical studies with species and hybrids of the genus were started. The first study (item 3) was based on the minicuttings morphology evaluation of the three eucalyptus species (E. urograndis, E. benthamii and E. urophylla) in contact with IBA in talc and the subsequent application of biostimulant treatments (tannic acid and Algaren BZn®) used in isolation and/or in combination directly on minicuttings base. The second study (item 4) evaluated the performance of these same treatments with biostimulants in E. urograndis microcuttings on the shoots elongation stage in vitro and their subsequent acclimatization. Finally, the third study (item 5) was based on the biostimulant treatments use in E. urophylla microcuttings in acclimatization phase in ministuff. The biostimulants were applied at microcuttings base and at ministuff base (directly on the trays). In all experiments, data regarding morphophysiological parameters were collected and it was observed in the first and second experiments that tannic acid at 250mg/L concentration was the best treatment used. For the third experiment, the Algaren BZn® in the concentration of 1 ml/L, presented more favorable results. According to the rhizogenesis histological analyzes for all the experiments, it was verified that the adventitious roots developed presented cambial origin and had a direct vascular connection with the aerial part, indicating its functionality. This showed that, in general, the biostimulants addition optimized the production of clonal Eucalyptus seedlings.
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Avaliação do potencial do ácido tânico e do líquido extraído da castanha do caju (Anacardium occidentale L) como antioxidante para biodieselMendes, Danylo Bezerra 04 April 2013 (has links)
A estabilidade oxidativa é uma das mais relevantes propriedades que atestam a qualidade de um biodiesel. Esse fato pode comprometer a armazenagem e a sua utilização como combustível e para prevenir esta oxidação, faz-se necessário o uso de compostos que demonstrem eficiência frente ao processo oxidativo em biodiesel. Assim sendo, o presente trabalho teve como objetivo geral avaliar o efeito do Ácido Tânico (AT) e do Líquido extraído da Castanha do Caju (Anacardium occidentale L.) (LCC) como antioxidante para biodiesel, analisando as características gerais da oxidação. Para tanto, foi utilizada uma metodologia onde o estudo da estabilidade do biodiesel foi realizado por meio do Rancimat, termogravimetria, espectrofotometria e análises de acidez. Por meio da análise no Rancimat, constatou-se que todos os biodieseis aditivados apresentaram um aumento do PI (período de indução), ou seja, um aumento da estabilidade oxidativa, de forma progressiva com o aumento da adição do antioxidante. A avaliação termogravimétrica (TGA) empregada demonstrou que o LCC técnico apresentou uma maior temperatura inicial e menor temperatura final de decomposição quando comparado com os demais antioxidantes testados. Os resultados dos testes de acidez e espectrofotometria na armazenagem em estufa e prolongado, com todos os antioxidantes testados, não mostraram aumento significativo da oxidação devido ao tipo de teste empregado, que buscou reproduzir as condições reais de armazenagem. Vale ressaltar que os resultados encontrados nesta pesquisa atendem aos parâmetros de qualidade exigidos pelo órgão regulador dos biocombustíveis, a ANP. / Oxidative stability is one of the most important properties that attest to the quality of a biodiesel. This fact can affect the storage and their use as fuel and to prevent this oxidation, it is necessary the use of compounds that show efficacy against oxidative process into biodiesel. Thus, the present work had as general objective to evaluate the effect of Tannic Acid (TA) and the liquid extracted from the Cashew Nut (Anacardium occidentale L.) (LCC) as antioxidant for biodiesel, analyzing the General characteristics of oxidation. To this end, we used a methodology where the study of the stability of biodiesel was performed by means of Rancimat, Thermogravimetry, spectrophotometry and the analysis of acidity. By means of Rancimat analysis, it was found that all additives biodieseis showed an increase of PI (induction period), an increase of oxidative stability, progressively increasing the addition of antioxidant. The thermogravimetric maid evaluation showed that the LCC a higher initial temperature presented technical and minor final temperature of decomposition when compared with other antioxidants tested. The results of the tests of acidity and spectrophotometry in the kiln and prolonged storage, with all the antioxidants tested, showed no significant increase in oxidation due to the type of test used, which sought to reproduce the actual conditions of storage. It is worth noting that the results found in this research meet the quality parameters required by the regulator of biofuels, the ANP.
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Production Of Tannase By Aspergillus NigerOzturk, Alev Deniz 01 July 2006 (has links) (PDF)
ABSTRACT
PRODUCTION OF TANNASE BY ASPERGILLUS NIGER
Ö / ztü / rk, Alev Deniz
M.S., Department of Chemical Engineering
Supervisor: Prof. Dr. Ufuk Bakir
Co-Supervisor: Prof. Dr. B. Zü / mrü / t Ö / gel
August 2006, 90 pages
In this study, a filamentous fungus, Aspergillus niger was evaluated in terms of extracellular tannase production. The effect of tannic acid, glucose and nitrogen sources on tannase and biomass productions was investigated and their concentrations were optimized. The highest enzyme activity was recorded as 316 U/ml in the optimized medium containing 8% Tannic acid, 1% Glucose, 0.4% (NH4)2HPO4, 0.1% K2HPO4, 0.1% MgSO4.7H2O, 0.01% ZnSO4.7H2O, 0.0005% NaCl in a shake-flask bioreactor at 35oC and 175 rpm.
The bioreaction profile including tannic acid, gallic acid, pyrogallol, glucose concentrations, pH, biomass and extracellular tannase production were determined under the optimized conditions. The maximum extracellular tannase activity (316 U/ml) was observed on the 4th day of cultivation. However, biomass continued to increase up to the 9th day of fermentation. Increase in biomass concentration during the first two days and after the 7th day was high. The microorganism used tannic acid and glucose during the first two days by considering the sharp decrease in tannic acid and glucose concentrations. The increase in biomass concentration after the 7th day was directly proportional to the decrease in pyrogallol concentration in this period of time. The pH of the cultivation medium decreased from 5.5 to 2.3 owing to the assimilation of glucose and the production of gallic acid.
Keywords: Tannase, Aspergillus niger, Enzyme production, Cultivation profile, Tannic acid.
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Μελέτη της επαγωγής σουπεροξειδικών ανιόντων και οξειδίων του αζώτου σε αιμοκύτταρα του μυδού Mytilus galloprovincialis (Lmk.), μετά από έκθεση σε μικρομοριακές συγκεντρώσεις βαρέων μετάλλων, παρουσία φαινολικών ενώσεωνΜπούκη, Ευδοκία 22 May 2013 (has links)
Οι πολυφαινόλες είναι μια κατηγορία οργανικών χημικών ουσιών, που
χαρακτηρίζονται από την παρουσία ενός ή περισσότερων υδροξυλίων, συνδεδεμένων
με έναν ή περισσότερους αρωματικούς ή ετεροκυκλικούς δακτυλίους φαινόλης. Το
ταννικό οξύ (ΤΑ), μια ειδική εμπορική μορφή της τανίνης, χρησιμοποιείται ευρέως
στη βιομηχανία και αποτελεί μία από τις κυριότερες ουσίες των βιομηχανικών
λυμάτων που διοχετεύονται στα υδάτινα οικοσυστήματα και στο έδαφος. Παρόλο το
γεγονός ότι οι πολυφαινόλες δρουν αντιοξειδωτικά στα κύτταρα, η ισορροπία μεταξύ
της αντιοξειδωτικής και της οξειδωτικής δράσης τους είναι υπό διερεύνηση. Η
παρούσα μελέτη διερευνά τις αντιοξειδωτικές και οξειδωτικές επιπτώσεις του
ταννικού οξέος σε αιμοκύτταρα του μυδιού Mytilus galloprovincialis, παρουσία
τοξικών συγκεντρώσεων καδμίου. Συγκεκριμένα, αιμοκύτταρα που εκτέθηκαν σε
διαφορετικές συγκεντρώσεις ΤΑ (1, 10, 20, 40 και 60 μΜ) για 1 h, έδειξαν σημαντική
μείωση της βιωσιμότητάς τους, μόνο σε συγκεντρώσεις ΤΑ μεγαλύτερες από 40 μΜ.
Παράλληλα, αιμοκύτταρα που εκτέθηκαν σε μικρομοριακές συγκεντρώσεις του
μετάλλου (50 και 100 μΜ) έδειξαν σημαντική μείωση της βιωσιμότητάς τους.
Προκειμένου να προσδιορίσουμε την αντιοξειδωτική ή οξειδωτική ικανότητα του ΤΑ,
αιμοκύτταρα που είχαν προηγουμένως προ-επωαστεί σε διαφορετικές συγκεντρώσεις
ΤΑ για 15 min, εκτέθηκαν σε μικρομοριακές συγκεντρώσεις του μετάλλου. Σύμφωνα
με τα αποτελέσματά μας, κύτταρα που είχαν προ-επωαστεί σε συγκεντρώσεις ΤΑ 1-
40 μΜ, έδειξαν σημαντική αναστολή των οξειδωτικών επιπτώσεων του μετάλλου
(παραγωγή σουπεροξειδικών ανιόντων/∙O2
−, οξειδίων του αζώτου/ ΝΟ, και
προϊόντων λιπιδικής υπεροξείδωσης/ επίπεδα μηλονικής διαλδεϋδης), όσο και της
ικανότητάς του να μειώνει τη βιωσιμότητα των κυττάρων, συγκριτικά με τις τιμές που
μετρήθηκαν σε κύτταρα που εκτέθηκαν μόνο στο μέταλλο. Αντίθετα, σε κύτταρα που
προ-επωάστηκαν σε ΤΑ 60 μΜ, πριν την έκθεσή τους στο μέταλλο, το ΤΑ
εμφανίστηκε να δρα συνεργατικά με το μέταλλο. Τα αποτελέσματα της παρούσας
μελέτης οδηγούν στο συμπέρασμα ότι το ΤΑ σε μικρομοριακές συγκεντρώσεις 1-40
μΜ μπορεί να δράσει ως ένας ισχυρός αντιοξειδωτικός παράγοντας, ενώ σε
υψηλότερες συγκεντρώσεις μπορεί να προκαλέσει οξειδωτικές επιπτώσεις, ανάλογες
με αυτές που προκύπτουν από ισχυρούς οξειδωτικούς παράγοντες, όπως τα κάδμιο. / Polyphenols are well-known organic substances, mainly characterized by the presence
of one or more hydroxyl groups on one or more aromatic or heterocyclic phenol rings.
Tannic acid (TA), a specific commercial form of tannin is a natural polyphenol,
widely used in food, pharmaceutical, leather and chemical industry. It is one of the
main organic compounds of industrial effluents discharged into aquatic ecosystems
and soil, causing environmental pollution. Despite the fact that a lot of studies
reported that polyphenols could act as antioxidants in different cells, the balance
between their antioxidant and pro-oxidant properties remains still unclear. According
to the later, the present study investigates the antioxidant and pro-oxidant potency of
TA in haemocytes of mussel Mytilus galloprovincialis in the presence or the absence
of micromolar concentrations of cadmium (Cd). Specifically, haemocytes exposed to
different concentrations of TA (1, 10, 20, 40 and 60 μΜ) for 1 h, showed a significant
reduction of their viability, only in concentrations higher than 40 μΜ. Furthermore,
cells exposed to micromolar concentrations of Cd (50 and 100 μΜ), showed
significantly increased levels of cell death, compared to those observed in control
cells. In order to investigate the antioxidant or pro-oxidant ability of TA, haemocytes
pre-treated for 15 min with different concentrations of TA were exposed to
micromolar concentrations of the metal. According to the results, cells pre-treated
with TA 1-40 μΜ, showed a significant attenuation of Cd induced effects, such as the
production of superoxide (∙O2
−) and nitric oxides (NO), MDA content as well as cell
death, compared to those occurred in the presence of the metal alone. On the contrary,
in cells pre-treated with TA 60 μΜ before their exposure to the metal, TA seemed to
act synergistically with the metal. The results of the present study could lead to the
suggestion that TA in concentrations ranged within 1 and 40 μΜ could act as a major
antioxidant factor, whereas in higher concentrations TA could cause oxidant effects,
similar with those caused by well-known pro-oxidants, such as cadmium.
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Tilapia skin Gelatin: Alternative for obtaining films with antimicrobial property by nanosilver incorporation / Gelatina de pele de tilÃpia: Alternativa para obtenÃÃo de filmes com propriedade antimicrobiana por incorporaÃÃo de nanoparticulas de prataMaria do Livramento Linhares Rodrigues 10 February 2015 (has links)
CoordenaÃÃo de AperfeiÃoamento de NÃvel Superior / An alternative for the recovery of industrial fish waste is the production of films, which can be applied in food packaging. However, a major challenge is to obtain films with antimicrobial properties aiding in increased food shelf life as well as films with good mechanical properties and barriers. Therefore, in order to improve these limitations, silver nanoparticles were used due to its potential antimicrobial in food, and tannic acid (TA) as crosslinker in order to improve the mechanical and barrier properties. This study focuses in the extraction of gelatin from the Nile tilapia skin (Oreochromisniloticus) in order to produce gelatin films with embedded nanosilver (AgNPs) generated by green process and evaluate the addition of tannic acid (TA) in synthesis of AgNPs and mechanical properties of the films and barrier. A pre-treatment was conducted to obtain gelatin followed by a collagen denaturing. The characterization was done by gel strength, isoelectric point, electrophoretic analysis, fourier transform infrared (FTIR) and thermogravimetric analysis (TGA). The incorporation was performed by in situ method, the films were obtained with and without the presence of TA and AgNO3 concentrations were 0, 45, 90 and 180 mmol.L-1.The films were characterized by UV-Vis, FTIR, TGA, Mechanical properties (tensile strength and elongation at break), water vapor permeability (WVP), scanning electron microscopy (SEM) and antimicrobial analysis. It was possible to use gelatin as a reducing agent and stabilizer in the reduction of silver nanoparticles. Films produced with the addition of AT showed more suitable properties for the usage as packaging material for food, showing better WVP, water solubility and were more resistant to applied loads. In addition, they presented better antimicrobial activity. Therefore, it was possible to obtain the green AgNPs method through the incorporationof thegelatin matrix and to obtain films with excellent properties after the TA addition. / Uma alternativa de valorizaÃÃo de resÃduo de pescado produzidos por indÃstrias à a produÃÃo de filmes, que pode ser aplicados em embalagens para alimentos. PorÃm, um dos grandes desafios à obter filmes com propriedades antimicrobianas, auxiliando no aumento da vida de prateleira dos alimentos, bem como obter filmes com boas propriedades mecÃnicas e de barreiras. Para isso, a fim de melhorar essas limitaÃÃes, nanopartÃculas de prata foram utilizadas devido a sua potencial aÃÃo antimicrobiana em alimentos, e Ãcido tÃnico (AT) como agente reticulante a fim de melhorar as propriedades mecÃnicas e de barreiras. Nesse estudo objetivou-se fazer a extraÃÃo da gelatina a partir de pele de tilÃpia do Nilo (Oreochromis niloticus), visando a produÃÃo de filmes de gelatina incorporados com nanopartÃculas de prata (NPAg) geradas por processo verde e avaliar a adiÃÃo de Ãcido tÃnico (AT) na sÃntese das NPAg e nas propriedades mecÃnicas e de barreira dos filmes. Para a obtenÃÃo da gelatina foram feitos prÃ-tratamentos e em seguida a desnaturaÃÃo do colÃgeno. A caracterizaÃÃo foi feita por ForÃa de Gel, Ponto IsoelÃtrico, DistribuiÃÃo de Massa Molar, Espectroscopia no Infravermelho pro Transformada de Fourrier (FTIR) e AnÃlise TermogravimÃtrica (TGA). A incorporaÃÃo foi feita pelo mÃtodo in situ, Os filmes foram obtidos sem e com a presenÃa de AT e as concentraÃÃes de AgNO3 foram 0, 45, 90 e 180 mmol.L-1. Os filmes foram caracterizados por: UV-Vis, FTIR, TGA, Ensaios MecÃnicos, Permeabilidade ao Vapor de Ãgua (PVA), Microscopia EletrÃnica de Varredura (MEV) e AnÃlise Antimicrobiana. Foi possÃvel utilizar a gelatina como agente redutor e estabilizante na reduÃÃo de nanopartÃculas de prata. Os filmes produzidos com a adiÃÃo de AT apresentaram propriedades mais adequadas para uso como embalagem para alimentos, apresentando melhores PVA, solubilidade em Ãgua e mostraram-se mais resistentes a esforÃos aplicados. AlÃm de apresentarem melhor atividade antimicrobiana. Logo, foi possÃvel, obter pelo mÃtodo verde a incorporaÃÃo de NPAg na matriz de gelatina, alÃm, de se obter filmes com excelentes propriedades apÃs a adiÃÃo de AT.
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