Global ETD Search

1	Intracellular pH, the Proximate Signal for Cell Volume Changes that are Mediated by the Actin Cytoskeleton Pasley, William 01 January 2005 (has links) The relationship between initial intracellular pH (pHi) and associated cell volume change was investigated by simultaneous measurement of pHi and cell volume with fluorescence imaging in polarized fungiform taste receptor cells (TRCs) loaded with BCECF in vitro. Ammonium pulses caused a brief, reversible alkalinization in pHi and induced cell swelling. Sodium-acetate pulses reversible decreased TRC pHi and induced cell shrinkage. Removal weak acids and return to Control Ringer's solution (CR) causedTRC pHi and volume to overshoot baseline levels before fully recovering. Replacing CR with zero-sodium solution resulted in irreversible acidification of TRC pHi and induced cell swelling. Addition of sodium allowed reversal of TRC pHi and volume and return to baseline levels. Treating TRCs with cytoskeleton inhibitors, phalloidin and cytochalasin, before acidic stimulation did not affect TRC pHi, but did result in an altered TRC volume change. I conclude that a decrease in TRC pHi induces cell shrinkage via the actin cytoskeleton. Cell shrinkage as a result of a change in pHi activates NHE1 to restore TRC pHi and volume. gustation taste receptors actin intracellular cytoskeleton taste papillia pHi TRC taste bud sodium phalloidin cytochalsin Psychology Social and Behavioral Sciences
2	Mosaic Analysis with Double Markers (MADM) as a Method to Map Cell Fates in Adult Mouse Taste Buds. Moore, Preston D. 18 December 2010 (has links) (PDF) Taste buds are chemosensory endorgans embedded in the oral epithelium composed of cells that undergo continuous replacement. Mature taste cells live on average 10-14 days and are replaced by new cells when they die. However, the mechanism by which taste cells are produced and integrated into the taste bud as mature taste cells remains unknown. Previous studies approached this issue from either cell cycle gene expression properties or lineage tracing of precursor cells. In our study, we apply a new fate mapping technique that combines these two ideas. This technique, Mosaic Analysis with Double Markers, allows for simultaneous gene knockout and subsequent tracking of single cells. This allows us to study the potency of precursor cells supplying the taste bud while analyzing how gene function regulates the maturation pathway these taste cells take. The following experiments illustrate the initial phase of this investigation. taste cell p27 taste bud Mosaic Analysis with Double Markers fate mapping MADM taste lineage tracing Cell and Developmental Biology Developmental Biology Life Sciences
3	The Physiological Role of Serotonergic Transmission in Adult Rat Taste Buds Jaber, Fadi Luc 21 May 2013 (has links) No description available. Neurosciences Serotonin 5-HT 5-HT1A 5-HT3 taste bud gustation chorda tympani immunocytochemistry single cell RT-PCR ATP P2X P2Y purinergic receptors T2R T1R

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