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Both global and local chronic environmental stressors associated with climate change and anthropogenic sources are increasing in severity, and can compromise the resilience of key marine ecosystems such as foundational coral reefs. Despite the impacts, however, there are major knowledge gaps in our understanding of direct interactive and compounding effects of multiple chronic environmental stressors on coral animals. Further, chronic stress may have sublethal, downstream impacts; for example, inhibiting the recognition and response to sudden acute stressors in the marine environment. The goal of this study was to determine the direct impacts of multiple chronic environmental stressors - elevated temperature (global), microplastic pollution, light availability, and food availability (local) - on survival and physiological performance of the emerging temperature coral model Astrangia poculata, and to determine how exposure to different chronic stressors affects their ability to deal with sudden acute stress. To achieve this, we exposed individual coral polyps to different combinations of stressors, and quantified the response of the coral host (growth) and symbiont (photosynthetic efficiency, chlorophyll a density). Coral polyps were then challenged with an acute stress near the onset, midpoint, and end of the experiment to quantify the impact of chronic environmental stress on the ability to mount a response to an acute stress. We found that the local stress of microplastic exposure had no impact on lethal or sublethal measures of the coral holobiont, while light was beneficial in maintaining coral mass. In contrast, elevated temperatures (representing global stress) reduced survival, diminished host and symbiont performance, and repressed the coral metabolic response under acute stress challenge. Feeding, however, was beneficial in preserving symbiont function, but has consequences for fitness and coral growth when presented with thermal stress, lending support to the growing hypothesis that this established mutualism shifts towards parasitism in stressful environmental conditions. Despite the magnitude of these combined stressors, over 80% of coral polyps survived, highlighting the overall resilience of A. poculata to diverse environmental challenges. These findings underline the complexity with which anthropogenic stressors interact to affect coral survivorship and resilience to future global change.

Interactions are important drivers of selection and community structure, which makes the study of multi-species interactions critical for understanding the ecology and evolution of organisms. This dissertation includes four data chapters that examine the biotic and abiotic mechanisms that shape multi-species interactions in both tropical and temperate ecosystems. The first three data chapters (Chapters 2–4) were completed within a Neotropical rainforest in Costa Rica and focus on one plant genus, Piper (Piperaceae). The final data chapter (Chapter 5) was conducted within a working landscape of soybean (Glycine max) fields in eastern Maryland, USA. In Chapter 2, I explore intra- and inter-specific dietary niche partitioning of Piper fruits among three frugivorous bats, illustrating the importance of fine-scale mechanisms that facilitate species coexistence and influence plant–animal interactions. In Chapter 3, I demonstrate how the chemical ecology of a Neotropical shrub, Piper sancti-felicis, shapes fruit interactions with antagonists (fruit fungi) and mutualists (frugivorous bats and birds), developing a foundation for understanding evolutionary ecology of plant chemical traits based on phytochemical investment patterns. In Chapter 4, I describe the direct and indirect impacts of elevated temperature and CO2 concentration on the plant traits and interactions in Piper generalense, improving our understanding of the effects of climate change on a Neotropical plant–herbivore system. In Chapter 5, I explore the biotic (herbivore-induced plant volatiles) and abiotic (fine-scale weather conditions) drivers affecting insectivorous bat foraging in soybean fields in eastern Maryland, providing a pathway to further investigate new strategies for integrated pest management. As a collective work, this dissertation disentangles the nuances of multi-species interactions, exploring foundational mechanisms underlying biodiversity maintenance as well as answering applied questions to address a changing climate and aid sustainable agriculture. / Doctor of Philosophy / Everything in nature is connected, so studying ecological interactions requires us to view them from many different angles. As with most relationships, ecological interactions are multi-faceted and context-dependent. In this dissertation, I describe both tropical and temperate systems, collecting a variety of measurements from plants, microbes, and animals to explore the complicated relationships that exist between them. In Chapter 2, I explore how three species of fruit-eating bats may divide the use of a shared food resource (tropical pepper fruits in the genus Piper) to maintain separate populations and how those foraging differences may affect Piper plant populations. In Chapter 3, I characterize a chemical compound found in the fruits of a Piper plant species and test the effect of that compound on fruit fungi and fruit-eating bats and birds, leading to a better understanding of the selective pressures affecting fruit chemistry. In Chapter 4, I describe the direct and indirect effects of climate change on a Piper plant in the first study to measure the responses of tropical understory plants to treatments that mimic climate change using active warming and CO2 supplementation. In Chapter 5, I explore the fine-scale drivers of bat activity in soybean fields, including how weather conditions and the specific compounds emitted by insect-damaged plants may affect bat activity. As a collective work, this dissertation describes the complex relationships among plants and their many interactors, exploring questions from biodiversity maintenance to integrated pest management strategies.

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Biogenic volatile organic compounds (BVOC) are a wide-ranging group of trace gas components in the atmosphere which are emitted naturally from Earth’s surface. It is now recognised that biogenically sourced VOCs are far more significant on a global scale than those from anthropogenic sources, with up to 10 times greater emissions. Very few field-based studies of fluxes from plant canopies have been undertaken, particularly for non-terpenoid compounds. This thesis presents mixing ratio and flux measurements of BVOC from a range of temperate plant canopies: Douglas fir, short-rotation coppice willow, Miscanthus and mixed peatland vegetation. The virtual disjunct eddy covariance technique (vDEC) using a proton transfer reaction mass spectrometer (PTR-MS) as a fast VOC sensor was used for all measurements except for peatlands, where grab samples were collected on adsorbent sampling tubes for later chromatographic analysis. The PTR-MS was also utilised for measuring the rate of degradation of VOCs during laboratory chamber experiments. Mixing ratios and fluxes of VOCs measured within and above a Douglas fir forest were the first canopy-scale measurements for this species. Fluxes of monoterpenes were comparable to previous studies while isoprene was also detected (standard emissions factors up to 1.15 μg gdw -1 h-1 and 0.18 μg gdw -1 h-1, respectively). Emissions of oxygenated VOCs were also found to be significant, highlighting the importance of quantifying a wider variety of VOCs from biogenic sources, other than isoprene and monoterpenes. Results for bioenergy crops Miscanthus and willow showed that willow was a high isoprene emitter (20 μg gdw -1 h-1), but no measureable VOCs were detected from Miscanthus. This indicates that future expansion of bioenergy crops, and hence species selection, should take resultant air quality and human health impacts – due to changing VOC emissions – into account. Fluxes of BVOC from a Scottish peatland are the first reported measurements for this ecosystem in a temperate climate. Additionally, to assess the impact of nitrogen deposition on VOC fluxes, BVOC measurements were taken from sample plots in a pre-existing, long-term field manipulation study to assess impacts of wet nitrate or ammonium deposition on peatland. The peatland was found to be a significant source of isoprene and monoterpenes (590 and 1.5 μg m-2 h-1 respectively) and there was evidence that emissions were affected by wet nitrogen treatment. Isoprene emissions were reduced by both nitrate and ammonium treatment, while nitrate increased β- pinene fluxes. Increasing atmospheric nitrogen concentrations are therefore predicted to have an impact on VOC emission. Chamber studies showed that the rate of loss of α-pinene from the gas-phase during oxidation – and hence potential formation of secondary organic aerosol (SOA) – decreased with increasing isoprene mixing ratio. This was not observed for limonene. These results show that as isoprene mixing ratios increase with increasing global temperatures, negative feedback on radiative forcing from SOA particles may be suppressed. Results from this thesis provide valuable experimental data for a range of temperate plant canopies, which will help constrain modelled predictions of future VOC emissions. Additionally, the importance of understanding the effects of land use and environmental change on VOC emissions was demonstrated.

Light spectra distributions were measured in two different montane forests: temperate and tropical. Spectral light measurements were made in different sized canopy gaps in the conifer forest at H. J. Andrews Experimental Forest in Oregon, USA. Researchers at Oregon State University created these gaps of 20 m, 30 m, and 50 m in diameter. In the tropical cloud forest, spectral light measurements were made in two plots that were permanently established at La Mucuy Parque Nacional in Venezuela, in collaboration with researchers at Universidad de Los Andes. In both studies, spectra and distributions of physiologically active light were analyzed: red, far-red, R/FR ratio, and blue light.

Early function of a temperature-sensitive mutant of staphylophage 44A HJD was examined during the twenty-five-minute period following infection. Host cell and phage DNA were labeled with C and3H respectively. DNA was separated into linear and covalently closed circular (CCC) forms by density-gradient centrifugation. The host, S. aureus, shows no CCC DNA, and apparently carries no plasmid. Following infection with wild type phage, CCC DNA forms occur in tritiated and 1 C DNA fractions 10 to 15 min after infection. Infection with mutant at permissive temperature also demonstrates CCC DNA with both labels. Infection with mutant at nonpermissive temperature produced no CCC DNA during the first 25 min after infection. The impaired function in this mutant may be a linker protein.

Woody plant expansion has been documented for decades in many different ecosystems, often due to anthropogenic disturbances to the environment and yielding vast changes in ecosystem functioning. While causes and consequences of woody expansion have been well documented, few studies have investigated functional traits of woody species that promote rapid expansion in range. My objective was to determine if hydraulic efficiency confers enhanced photosynthetic efficiency so that functional traits representing light and water use may be possible mechanisms facilitating woody encroachment into grasslands and forest understories. I quantified leaf-level light environment, photosynthetic activity, and hydraulic characteristics of three sympatric broadleaf evergreens of varying leaf life span (Ilex opaca, Kalmia latifolia, and Myrica cerifera) in a deciduous forest understory to understand seasonal intra- and interspecific ranges of broadleaf evergreen physiology. Additionally, I investigated the effects of age on physiological efficiency of M. cerifera across a chronosequence (i.e., space for time substitution) of shrub thicket development in order to understand possible age-related physiological mechanisms facilitating shrub expansion. Lastly, I determined functional traits and resulting physiology that contribute to rapid expansion and thicket formation of an invasive, deciduous, N-fixing shrub, Elaeagnus umbellata, and a native, evergreen, N-fixing shrub M. cerifera. When compared to co-occurring evergreen species, electron transport rate (ETR) of M. cerifera was nearly double that of I. opaca or K. latifolia in summer. Photosynthetic capacity was positively related to hydraulic capacity among understory evergreens. Furthermore, photosynthetic and hydraulic efficiency of M. cerifera remained consistent despite considerable differences in thicket age and development. Both similar and contrasting functional traits of E. umbellata and M. cerifera allowed for enhanced light capture and water movement, and reductions in subcanopy light penetration. Enhanced hydraulic and photosynthetic efficiency relative to co-occurring species contributes to rapid range expansion and thicket formation by promoting enhanced productivity and limiting successful colonization of other species. My results indicate that there may be suites of functional traits linked to expansive success and thicket-formation, yet differences in functional traits between native and invasive species represent alternative strategies leading to rapid growth and thicketization.

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