No description available.

Thesis (Ph.D.)--Boston University / The subject of this investigation was the staphylococcal substance which we now call enterotoxin and which we know to be responsible for staphylococcal food poisoning in man. The immunological response of animals to this substance, as well as the exceptional heat resistance of the enterotoxin, indicates that it is indeed quite different from other known bacterial exotoxins. It was hoped that this study of some of the physical and chemical properties of the enterotoxin would help to explain those differences and also yield information about the chemical nature of the toxin itself. [TRUNCATED]

No description available.

No description available.

Thesis (Ph. D.)--University of Wisconsin, 1965. / Typescript. Vita. eContent provider-neutral record in process. Description based on print version record. Includes bibliography.

Thesis (Ph. D.)--University of Chicago, 1940. / Reproduced from type-written copy. "Private edition, distributed by the University of Chicago libraries, Chicago, Illinois." Bibliography: p. 25-26.

No description available.

The interaction between protein A and immunoglobulin G (IgG) was studied at a variety of pH values using a surface plasmon resonance (SPR) device, which provides real time kinetic data without labelling or molecular alteration. This study was carried out due to the large scale use of Protein A affinity chromatography for the purification of IgG for pharmaceutical purposes, and is one of the most costly steps in the purification process. The results produced were largely in line with those produced in previous literature with binding remaining strong between pH 7.4 and 5.0, although the association rate decreased as pH decreased. Below pH 5.0, the rate of IgG elution markedly increased, with pH 3.5 showing near full elution seconds after the association phase of the SPR interaction finished. Problems were encountered with non-specific binding between the SPR sensor chip and IgG occurring under a variety of conditions, requiring various remedies. However, no complete interactions were successfully carried out under pH 5.0, so the results obtained below this value were obtained by binding at pH 7.4 and then elution at the desired pH. The data showed binding behaviour that was most successfully explained by a three-site model, each with a binding ratio of 1:1. The binding ratio is questionable given that Protein A and IgG typically bind at a ratio of 1:2 but may be explained by the sites being independent of one another and thus no secondary attachment is observed. A variety of models were fitted to the data but only two- and three-site models fitted the experimental data, with the three-site model being a more accurate and robust fit across pH changes. A multiple site model seems intuitively correct given the six different binding sites that Protein A has for interaction with IgG. The models produced have potential applications in a larger model of Protein A affinity chromatography, although a number of additional factors would need to be taken into account, such as mass transfer effects and the IgG concentration gradient.

Staphylococcal tenosynovitis and osteomyelitis are world-wide problems of broilers and broiler breeders caused by staphylococci. Pathogenesis of the disease is ill defined. Avian heterophils are analogous to mammalian neutrophils but the granules appear to be different. The first chemotactic study was done on heterophils from chickens having natural staphylococcal infection brought from a commercial broiler flock and on the heterophils obtained from healthy 6-8 weeks old chickens brought from a local hatchery as one day old chicks. In the second study, a chemotactic study was done with three different staphylococci on heterophils obtained from healthy 6-8 weeks old chickens brought from a local hatchery as one day old chicks. Results for the first study showed a decreased chemotactic response in the heterophils of chickens naturally infected with staphylococcus compared to healthy chicken heterophils in response to minimum essential medium, pooled normal chicken serum and E. coli endotoxin with normal chicken serum used as chemoattractants. Second study results showed that pathogenic capsule type 5 and type 8 Staphylococcus aureus isolates both induced chemotaxis in heterophils from healthy chickens to a significantly greater degree than did a non-pathogenic Staphylococcus xylosus. The Staphylococcus aureus isolate with capsule type 5 induced heterophil chemotaxis more than the capsule type 8 isolate. / Graduation date: 1993

Thesis (Ph. D.)--University of Sydney, 2000. / Title from title screen (viewed Apr. 21, 2008). Submitted in fulfilment of the requirements for the degree of Doctor of Philosophy to the Faculty of Dentistry. Degree awarded 2000; thesis submitted 1999. Includes bibliographical references. Also available in print form.