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Thermus thermophilus Argonaute Functions in the Completion of DNA ReplicationJolly, Samson M. 20 May 2020 (has links)
Argonautes (AGOs) are present in all domains of life. Like their eukaryotic counterparts, archaeal and eubacterial AGOs adopt a similar global architecture and bind small nucleic acids. In many eukaryotes, AGOs, guided by short RNA sequences, defend cells against transposons and viruses. In the eubacterium Thermus thermophilus, the DNA-guided Argonaute TtAgo defends against transformation by DNA plasmids. We find that TtAgo also participates in DNA replication. In vivo, TtAgo binds 15–18 nt DNA guides derived from the chromosomal region where replication terminates, and TtAgo complexed to short DNA guides enhances target finding and prefers to bind targets with full complementarity. Additionally, TtAgo associates with proteins known to act in DNA replication. When gyrase, the sole T. thermophilus type II topoisomerase, is inhibited, TtAgo allows the bacterium to finish replicating its circular genome. In contrast, loss of both gyrase and TtAgo activity slows growth and produces long, segmented filaments in which the individual bacteria are linked by DNA. Furthermore, wild-type T. thermophilus outcompetes an otherwise isogenic strain lacking TtAgo. Finally, at physiologic temperature in vitro, we find TtAgo possesses highest affinity for fully complementary targets. We propose that terminus-derived guides binding in such a fashion localize TtAgo, and that the primary role of TtAgo is to help T. thermophilus disentangle the catenated circular chromosomes generated by DNA replication.
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Spatial Patterns of Molecular Traits in Bacterial Genomes / Bacterial Molecular Properties and Genomic PositionLato, Daniella Fiora January 2021 (has links)
The placement of genetic information within bacterial genomes is intentionally organized, creates predictable gradients of molecular properties along the origin-terminus of replication axis. Previous studies have reported that genes located near the origin of replication generally have a higher expression level, increased dosage, and are more conserved than genes located near the terminus of replication. Additionally, substitution rates usually increases with increasing distance from the origin of replication. However, the constant reorganization of genetic information is often overlooked when considering spatial molecular trends.
Here, we explore the interplay of genomic reorganization along the origin and terminus of replication axis of gene expression and substitutions in Escherichia coli, Bacillus subtilis, Streptomyces, and Sinorhizobium meliloti. Using ancestral reconstruction to account for genome reorganization, we demonstrated that the correlation between the number of substitutions and distance from the origin of replication is significant but small and inconsistent in direction. In another study, we looked at the overall expression levels of all genes from the same bacteria, and
confirmed that gene expression tends to decrease when moving away from the origin of replication.
We looked specifically at how inversions - one type of genomic reorganization - impact gene expression between closely related strains of E. coli. Some inversions cause significant differences in gene expression compared to non-inverted regions, however, the variation in expression does not significantly differ between inverted and non-inverted regions. This change in gene expression may be due to the expression regulation properties of two nucleoid proteins, Histone-like Nucleoid-Structuring (H-NS) and Factor for inversion stimulation (Fis), who’s binding sites had a significant positive correlation with inverted regions.
In conclusion, we highlight the impact that genomic rearrangements and location have on molecular trends in bacteria, illustrating the importance of considering spatial trends in molecular evolutionary analysis, and to ensure accurate generalization of previously determined trends. Assuming that molecular trends are exclusively in one direction can be problematic. / Dissertation / Doctor of Philosophy (PhD)
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