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Assessment of SPAG-1. a sporozoite surface antigen of Theileria annulata, as a vaccine candidateBoulter, Nicola Rosalind January 1996 (has links)
No description available.
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Methods for the design and evaluation of East Coast fever (ECF) control strategiesKimera, Sharadhuli I. January 1998 (has links)
No description available.
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A Theileria annulata sporozoite surface antigen as a potential vaccine for tropical theileriosisWilliamson, Susanna Margaret January 1989 (has links)
No description available.
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Molecular and biochemical characterisation of non-attenuated and attenuated isolates of Theileria annulataSomerville, Robert Peter Townshend January 1997 (has links)
No description available.
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The relationship between theileria parva parva and t.p. lawrencei as shown by sporozoite antigen and ribosomal RNA gene sequencesCollins, Nicola, Elaine January 1997 (has links)
A thesis submitted to the Faculty of Science,
University of the Witwatersrand, Johannesburg,
in fulfillment of the requirements for the degree
of Doctor of Philosophy. / The aim of this thesis was to develop DNA probes to distinguish between the
protozoan parasites Theileria parva parva and T. p. lawrencei which cause East
Coast fever (ECF) and Corridor disease respectively. ECF was eradicated from
South Arrlca in 1954, and today Corridor disease has become the most important
form of theileriosis. Although ECF has been eradicated, the vector ticks are still
prevalent in South Africa and the cattle population would be highly susceptible to a
recurrence of the disease, At present there is no reliable means of distinguishing
between T.p. parva and T. p. lawrencei.
Sequence differences between T. parva and other Theileria species have previously
been found in the small subunit ribosomal RNA (rRNA) gene; probes designed to
detect these sequence differences Can be used to distinguish between Theileria
species. We therefore decided to search for differences in the rRNA genes of
T. p. parva and T.p. lawrencei. To this end, the entire "RNA transcription unit was
amplified from a cloned T. p, lawrence; parasite; the unit comprises the small subunit
rRNA (SSUrRNA) gene, the internal transcribed spacer (ITS) and the large subunit
rRNA (LSUrRNA) gene. The amplification products were cloned and sequenced,
and the T.p, lawrencei rRNA sequence was compared to that of T. p, parva, While
there was little variation in their SSUrRNA and LSUrRNA gene sequences, there was
major sequence variation in the ITS The ITSs from twelve T. parva isolates were
amplified, cloned and sequenced, and eleven characterisation oligonucleotide probes
were identified. The T. p, parva isolates screened in this study hybridised with a
limited subset of the probes, While the T. p. lawrencei isolates, hybridised with many
more of the probes, indicating that the T. parva population in cattle is more
homogenous than that in buffalo. There thus appears to have been a selection in
cattle of a relatively homogenous subpopuiation of T. parva from a much larger,
more diverse gene pool in buffalo. Although most T.p. parva isolates (93.5%) were
detected by probe TPPI, and most T.p, lawrencei isolates (81.8%) were detected by / AC2017
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Aspects of tick immunobiologyOtim, C. P. January 1997 (has links)
No description available.
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Molecular detection and characterization of tick-borne pathogens of dogsMatjila, Paul Tshepo. January 2008 (has links)
Thesis (PhD. (Veterinary Science))--University of Pretoria, 2008. / Includes bibliographical references. Also available in print format.
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Occurrence of Theileria parva infection in cattle on a farm in KwaZulu-Natal, South AfricaThompson, Bronwen Eleanor 01 November 2007 (has links)
Theileriosis is a tick-transmitted protozoal disease caused by several Theileria species. The most virulent species affecting cattle are T. parva and T. annulata. Theileria parva is responsible for causing East Coast Fever (ECF), Corridor Disease and January Disease. Theileria parva is endemic in certain areas of South Africa, Zambia, Zimbabwe and elsewhere. In South Africa these areas are controlled, include mostly game reserves containing infected African buffaloes and are in declared Corridor disease infected districts. African buffaloes are natural reservoirs of the T. parva parasite and if suitable tick species (Rhipicephalus appendiculatus and R. zambeziensis) are present, cattle may become infected. Currently, T. parva is diagnosed by conducting an array of tests including Giemsa-stained blood and lymph node smears, indirect immunofluorescent antibody (IFA) test, standard PCR and PCR/DNA-probe test. Recent progress in the diagnosis of T. parva has involved the development of a Reverse-Line Blot (RLB) technique and a real-time PCR for detecting T. parva. Over the last decade, several outbreaks of T. parva have occurred in cattle in the province of KwaZulu- Natal. Between 2002 and 2004, Mr A. Green, a Red Brangus stud farmer in the Ladysmith district, lost a total of 42 cattle to T. parva infection. An investigation into the epidemiology of T. parva in KwaZulu-Natal was structured in three parts: (i) a survey of Red Brangus on Mr Green‟s farm in order to gain insight into the current prevalence of theileriosis and other tick-borne diseases in the herd; (ii) transmission experiments from T. parva-infected cattle on Mr Green‟s farm to susceptible cattle to demonstrate vector transmission as well as replicate the disease process and pathology; (iii) tick transmission experiments from persistently T. parva positive-testing buffalo and persistently T. parva negative-testing buffalo, to susceptible bovines to demonstrate suitability of tick transmission methods and compare pathology and clinical signs seen on the farm and in the tick transmission experiments. The survey demonstrated that several factors may interact in determining the number of infected animals, the period that they remain infective to ticks as well as the susceptibility of the rest of the herd to T. parva. Sampled bovines did not show clinical signs of T. parva-associated disease and with test results indicated that the number of subclinical carriers may be high. Attempts to transmit T. parva from infected cattle on Mr Green‟s farm to susceptible cattle were not successful. Transmission from an infected buffalo to a susceptible bovine was successful and transmission of T. parva from a negative-testing buffalo to a susceptible bovine could not be demonstrated. These experiments give some confidence that the tick-transmission methods and diagnostic tests used were reliable. It remains important to consider African buffalo as a possible source of T. parva infection in a cattle herd even when a direct link cannot be established. / Dissertation (MSc (Veterinary Science))--University of Pretoria, 2007. / Veterinary Tropical Diseases / MSc / unrestricted
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The occurrence of piroplasms in various South African black rhinoceros (Diceros bicornis) populationsZimmerman, David Edwin. January 2010 (has links)
Thesis (MSc (Veterinary Tropical Diseases)--University of Pretoria, 2009. / Includes bibliographical references. Also available in print format.
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Occurrence of Theileria parva infection in cattle on a farm in KwaZulu-Natal, South AfricaThompson, Bronwen Eleanor. January 2007 (has links)
Thesis (MSc (Veterinary Science)--University of Pretoria, 2007. / Includes bibliographical references.
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