Charles University in Prague Faculty of Pharmacy in Hradec Králové Department of Biochemical Sciences Candidate: Bc. Jana Stehnová Supervisor: Prof. MUDr. Jaroslav Dršata, CSc. Consultant: MUDr. Mgr. Sylvie Špitálníková, Ph.D. Title of diploma thesis: Laboratory investigation of thyreoidea in pregnancy Thyroid diseases are very common in the Czech Republic. It is reported that up to 46% of the population suffer with thyroid diseases. Women are afflicted with the desease 4 to 8 times more often than men, and particularly in pregnancy, hormones of the thyroid gland play an important role in developing, maintaining and progress of pregnancy. In this work, we focused on creating the reference intervals for TSH, fT4 and antiTPO in the first trimester of pregnancy, which are recommended as the main markers of thyroid assessment of thyroid function during pregnancy. Reference intervals created by us were compared with the recommended reference intervals of the supplied Roche diagnostics. Our reference file was compiled from a group of 81 pregnant women and divided into individual weeks of the first trimester (weeks 11, 12 and 13). Reference intervals were calculated according to the recommendation, such as 2.5th and 97.5th percentile of our reference group for each week and then the resulting average...

We present the case of a 7-month-old baby with Cushing’s disease due to an adrenocorticotropic hormone (ACTH)-secreting pituitary adenoma combined with cells producing thyreotropin-secreting hormone (TSH). In MRI scans, a contrast-enhancing lesion was seen inside the pituitary fossa, and it extended into the suprasellar region. On the assumption of a pituitary adenoma, surgery was performed. Corresponding with biochemical findings, histopathological evaluation revealed an ACTH- and TSH-producing tumor. Genetic analysis did not demonstrate an alteration at codon 201 (Arg) and 227 (Glu). To our knowledge, this is the first case described in a child of this age. / Dieser Beitrag ist mit Zustimmung des Rechteinhabers aufgrund einer (DFG-geförderten) Allianz- bzw. Nationallizenz frei zugänglich.

We present the case of a 7-month-old baby with Cushing’s disease due to an adrenocorticotropic hormone (ACTH)-secreting pituitary adenoma combined with cells producing thyreotropin-secreting hormone (TSH). In MRI scans, a contrast-enhancing lesion was seen inside the pituitary fossa, and it extended into the suprasellar region. On the assumption of a pituitary adenoma, surgery was performed. Corresponding with biochemical findings, histopathological evaluation revealed an ACTH- and TSH-producing tumor. Genetic analysis did not demonstrate an alteration at codon 201 (Arg) and 227 (Glu). To our knowledge, this is the first case described in a child of this age. / Dieser Beitrag ist mit Zustimmung des Rechteinhabers aufgrund einer (DFG-geförderten) Allianz- bzw. Nationallizenz frei zugänglich.