The competition between two molecules of similar polarity for adsorption sites on the stationary phase is discussed in light of rapid kinetics of adsorption and desorption, and of the effects of temperature, acetonitrile, surfactant (cyclohexylaminopropane sulfonic acid, CAPS) and salt concentrations on the retention of the thyroid hormones (3,5-diiodothyronine, T2; 3,3',5-triiodothyronine, T3 and thyroxine, T4). A three parameter equation relates the surfactant concentration and ionic strength to the retention of the hormones and is analyzed in terms of the Stern-Gouy-Chapman theory. A second order polynomial describes the temperature dependency and permits the evaluation of the enthalpy, entropy and heat capacity, demonstrating a reduction in the molecular motion of the analyte with increasing surfactant and acetonitrile concentrations. The equation parameters for linear or non-linear equations, using data sets with or without homogeneous variances, are evaluated using a Simplex optimization procedure that uses one of two proposed optimization criteria. The construction and operation of a computer based gradient programmer for HPLC is described. A surfactant mediated gradient elution with electrochemical detection is examined for the analysis of serum thyroid hormones.

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国立情報学研究所で電子化したコンテンツを使用している。

xv, 158 leaves : ill. ; 30 cm. / Title page, contents and abstract only. The complete thesis in print form is available from the University Library. / Thesis (Ph.D.)--University of Adelaide, Dept. of Animal Physiology, 1975

xv, 158 leaves : ill. ; 30 cm. / Title page, contents and abstract only. The complete thesis in print form is available from the University Library. / Thesis (Ph.D.)--University of Adelaide, Dept. of Animal Physiology, 1975

"Publication authorized September 2, 1943." / Includes bibliographical references (p. 69-75).

Thesis (Ph. D.)--Ohio State University, 2003. / Title from first page of PDF file. Document formatted into pages; contains xi, 123 p.; also includes graphics (some col.) Includes bibliographical references (p. 109-123). Available online via OhioLINK's ETD Center

Thesis (Ph. D.)--Ohio state University, 1926. / Autobiography. The first two articles are reprinted from Endocrinology, vol. ix, no. 5, and vol. x, no. 1, and the third from the American journal of physiology, v. 78, no. 2. Bibliography at end of each article.

Part of Thesis (Ph. D.)--University of Chicago, 1932. / "Private edition, distributed by the University of Chicago Libraries, Chicago, Illinois." Bibliography: p. 11-12.